Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Strains, Media, and Growth Conditions
2.3. Identification of Aap Homologs
2.4. DNA and RNA Preparation
2.5. Disruption of ChAAP Genes
2.6. ChDDO Induction Experiment
2.7. DDO Assay
2.8. Quantitative Real-Time RT-PCR (qRT-PCR)
2.9. Sequence Analyses and Structural Modeling
3. Results
3.1. Identification of Acidic Aap Homologs of C. humicola Strain UJ1
3.2. Growth Characteristics of Chaap4 and Chaap5 Strains on Amino Acids
3.3. Effect of Medium pH on the Growth of Chaap4 and Chaap5 Strains on Amino Acids
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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N Source | Chaap4 | Chaap5 | |
---|---|---|---|
None | Below Detection | Below Detection | |
NH4Cl | 95% | 98% | |
Aliphatic | l-Ala | 106% | 115% |
Gly | 108% | 115% | |
l-Ile | 95% | 107% | |
l-Val | 113% | 104% | |
Aromatic | l-Phe | 60% * | 79% * |
Sulfur | l-Met | 102% | 95% |
Hydroxylated | l-Ser | 106% | 106% |
Acidic | l-Asp | 36% *** | 94% |
d-Asp | 67% ** | 102% | |
l-Glu | 84% ** | 103% | |
Amide | l-Asn | 96% | 107% |
l-Gln | 102% | 108% | |
Basic | l-Lys | 96% | 92% * |
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Imanishi, D.; Kera, Y.; Takahashi, S. Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola. Microorganisms 2021, 9, 192. https://doi.org/10.3390/microorganisms9010192
Imanishi D, Kera Y, Takahashi S. Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola. Microorganisms. 2021; 9(1):192. https://doi.org/10.3390/microorganisms9010192
Chicago/Turabian StyleImanishi, Daiki, Yoshio Kera, and Shouji Takahashi. 2021. "Identification of an Acidic Amino Acid Permease Involved in d-Aspartate Uptake in the Yeast Cryptococcus humicola" Microorganisms 9, no. 1: 192. https://doi.org/10.3390/microorganisms9010192