Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance of Campylobacter spp.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Steers and Treatment
2.2. Gastrointestinal (GI) Fluid Collection, Active Drug Concentration Determination, and Pharmacokinetic Analysis
2.3. Sample Collection and Processing for Campylobacter
2.4. Campylobacter Species Determination, Multilocus Sequence Typing (MLST), and Minimum Spanning Tree (MST)
2.5. Campylobacter Antimicrobial Susceptibility and MIC Determinations
2.6. Statistical Analysis
3. Results
3.1. Impacts of CCFA and CHCL on Prevalence of Campylobacter spp.
3.2. Incubation Temperature Impacted the Estimated Prevalence of C. jejuni and Other Campylobacter spp.
3.3. The Prevalence of Campylobacter-Positive Samples Reflected the Drug Concentration in the Colon, But C. jejuni Ceftiofur MIC Was Not Impacted by Either Treatment
3.4. Isolates Resistant to Both Tetracycline and Kanamycin Predominated Pre- and Post-Treatment in BOTH Treatment Groups
3.5. Multilocus Sequence Typing (MLST) Analysis Suggests that CCFA Treatment May Favor the Diversity of C. jejuni
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fan, S.; Foster, D.; Miller, W.G.; Osborne, J.; Kathariou, S. Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance of Campylobacter spp. Microorganisms 2021, 9, 318. https://doi.org/10.3390/microorganisms9020318
Fan S, Foster D, Miller WG, Osborne J, Kathariou S. Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance of Campylobacter spp. Microorganisms. 2021; 9(2):318. https://doi.org/10.3390/microorganisms9020318
Chicago/Turabian StyleFan, Sicun, Derek Foster, William G. Miller, Jason Osborne, and Sophia Kathariou. 2021. "Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance of Campylobacter spp." Microorganisms 9, no. 2: 318. https://doi.org/10.3390/microorganisms9020318