Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria
Abstract
:1. Introduction
1.1. The Milk Fat Globule Membrane
1.1.1. Structure and Origin
1.1.2. Composition
1.2. Lactic Acid Bacteria (LAB) and Bifidobacteria
2. Interactions of Bifidobacteria, LAB, and MFGM in Dairy Food Matrixes
3. Evidence of Improved Health with Combined Probiotic and MFGM Supplementation
3.1. Bacterial Survival and Adhesion
3.2. Nutrient Absorption, Mucosal Immunity, and Gut Barrier Function
3.3. Neurodevelopment and Cognitive Function
4. Current Applications and Future Perspectives
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Component | Concentration Range (%) |
---|---|
Lipids | 64–71.8 |
Protein | 22.3–28 |
Glycoconjugates | 10 |
RNA | Traces |
Lipid Class | Fraction Content In % | Subclass |
---|---|---|
Polar Lipids | ||
Phospholipids | 65 | Glycerophospholipids
|
Gangliosides or glycolipids | 5 |
|
Neutral Lipids | ||
Di-acylglycerols | 10 | |
Mono-acylglycerols | Traces | |
Free fatty acids | 10 | |
Sterols | ||
Cholesterol | 10 | |
Liposoluble molecules | ||
Vitamins A, D, E and K | 5 |
Protein | Content in g/100 g of Total Protein * | Molecular Mass (kDa) | Reported Effect on Human Health |
---|---|---|---|
Mucin 1 (MUC-1) | NA | 250–450 | Antiviral and antibacterial by preventing binding of pathogens to intestinal cells |
Mucin 15 (MUC-15) | NA | 94–100 | Antiviral action |
Xanthine dehydrogenase/oxidase (XDH/XO) | 0.58 | 150–155 | Bactericidal action by production of hydrogen peroxide and nitric oxide |
Cluster of differentiation 36 (CD36) | 0.18 | 77–78 | Receptor for collagen and thrombospondin. Scavenger receptor for apoptotic cells |
Butyrophilin (BTN) | 3.35 | 66 | Member of the immunoglobulin superfamily, adhesive protein, acts as a receptor and has a positive effect in the immune system. Co-inhibitor of T-cell activation |
Adipophilin (ADPH) | 0.007 | 52 | Facilitates transport of triglycerides and fatty acids during fat globule synthesis |
PAS 6/7 or Lactadherin | 0.93 | 48–54 | Adhesive properties with effect in the regulation of epithelial coagulation. Role in synaptic activity in the central nervous system (CNS) and protection against viral infection in the gut |
FABP family | 0.17 | 14–15 | Transport of fatty acids |
Ingredient | Microorganism(s) | Model | Experimental Design | Key Findings | Ref. |
---|---|---|---|---|---|
Bacterial Survival and Adhesion | |||||
Whey-derived MFGM (MFGM-10 Lacprodan®) | L. rhamnosus GG (LGG) | Male, 6-week-old BALB/c mice | Oral gavage of 0.1 mL MRSC media, MRSC with 5 g/L MFGM-10, MRSC with LGG (5 × 107 CFU/mL) or MRSC with 5 g/L MFGM-10 and LGG (5 × 107 CFU/mL) for 3 days |
| [87] |
MFGM-derived MPL concentrate | Lacticaseibacillus casei OSU-PECh-C; Lactobacillus acidophilus Musallam2; L. plantarum subsp. plantarum TW14-1; L. delbruekii OSU-PECh-3 | Gold (Au) Sensor; Caco-2/HT29-MTX | Examined the adhesion phenomena of 4 strains in the presence or absence of 0.5% (w/v) MPL to A) a gold sensor using a Quartz Crystal Micrograph with Dissipation (QCM-D); B) TEM; and C) intestinal cell culture |
| [88] |
MFGM-derived MPL concentrate | P. acidilactici OSU-PECh-L; P. acidilactici OSU-PECh-3A; L. plantarum OSU-PECh-BB, L. reuteri OSUPECh-48; L. casei OSU-PECh-C, L. paracasei OSU-PECh-BA; L. paracasei OSU-PECh-3B | Caco-2 | LAB strains grown with or without 0.5% (w/v) MPL were characterized by functional properties and their adhesive ability to fully differentiated Caco-2 cells |
| [86] |
MFGM extract from butter serum | L. rhamnosus GG (LGG) | Caco-2 TC7 | LGG was exposed to 5 mg/mL MFGM extract for 1 h and applied to intestinal cells (1 × 109 CFU/mL) |
| [76] |
MPL-rich milk protein concentrate (Lacprodan® PL-20) | B. longum subsp. infantis ATCC 15697 | HT-29 | Exposed bifidobacteria to MFGM ingredients for 1 h and measured adherence of bacteria to fully confluent cells after 2 h incubation using plate count method |
| [89] |
Whey-derived MFGM (MFGM-10 Lacprodan®) | |||||
Buttermilk fraction (BF) | |||||
Nutrient Absorption, Mucosal Immunity, and Gut Barrier Function | |||||
MFGM-derived MPL concentrate | L. delbruekii subsp. bulgaricus 2038; S. thermophilus 1131 | Male Sprague-Dawley rats | Orally supplemented rats with SM, MPLs alone or either of these in fermented milk |
| [90] |
Whey-derived MFGM (MFGM-10 Lacprodan®) | L. paracasei subsp. paracasei F19 (F19) | Infants (21-days−4-months old) | Double-blind RCT for an infant formula supplemented with MFGM (5 g/L prepared formula) or F19 (1 × 108 CFU/L) |
| [91] |
Unspecified MFGM fraction | B. animalis subsp. lactis BB-12 (BB-12) | HT-29Cl34 (NF-κB reporter cell line); 28-day-old mice | Used reporter cell line to measure NF-κB activation in response to BB-12 (1 × 106 or 1 × 107 CFU/mL) and/or MFGM (50 μg/mL or 100 μg/mL) and LPS challenge (100 ng/mL); For in vivo mouse study, administered BB-12 (1 × 108 CFU/day), MFGM (0.6 mg/g of body weight/day), or both BB-12 and MFGM orally for 1 or 4 weeks |
| [92] |
Neurodevelopment and Cognitive Function | |||||
MFGM components | B. infantis IM1; L. rhamnosus LCS-742 | 12-month-old infants | Double-blind RCT (COGNIS study) for a novel infant formula containing bioactive ingredients, including MFGM [10% of total protein content (w/w)] and probiotics |
| [93] |
MFGM components | B. infantis IM1; L. rhamnosus LCS-742 | 4-year-old infants | Double-blind RCT (COGNIS study) for a novel infant formula containing bioactive ingredients, including MFGM [10% of total protein content (w/w)] and probiotics |
| [94] |
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Kosmerl, E.; Rocha-Mendoza, D.; Ortega-Anaya, J.; Jiménez-Flores, R.; García-Cano, I. Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria. Microorganisms 2021, 9, 341. https://doi.org/10.3390/microorganisms9020341
Kosmerl E, Rocha-Mendoza D, Ortega-Anaya J, Jiménez-Flores R, García-Cano I. Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria. Microorganisms. 2021; 9(2):341. https://doi.org/10.3390/microorganisms9020341
Chicago/Turabian StyleKosmerl, Erica, Diana Rocha-Mendoza, Joana Ortega-Anaya, Rafael Jiménez-Flores, and Israel García-Cano. 2021. "Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria" Microorganisms 9, no. 2: 341. https://doi.org/10.3390/microorganisms9020341