Encystation of Entamoeba histolytica in Axenic Culture
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. E. histolytica Culture Maintenance
2.3. Encystation and Sarkosyl Treatment
2.4. Viability and Excystation
2.5. Staining and Fluorescence Microscopy
2.6. Flow Cytometry Analysis
3. Results
3.1. Growth Conditions for Encystation
3.2. Tetranucleation
3.3. Chitin Cell Wall
3.4. Small Size and Roundedness
3.5. Detergent Resistance
3.6. Viability and Excystation
3.7. Role of Cell Density in Encystation
3.8. Effect of Propionate on Encystation
3.9. Effect of Other SCFAs on Encystation
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Starting Cell Density (cells/mL) | TYI Basal | TYI Propionate | p-Value: Basal vs. Propionate |
---|---|---|---|
Total cysts produced (× 107) | |||
10K | 2.29 ± 0.30 | 8.48 ± 0.93 | p = 0.0004 |
25K | 2.63 ± 0.55 | 8.04 ± 0.84 | p = 0.0007 |
50K | 6.07 ± 0.39 | 9.57 ± 0.16 | p = 0.0001 |
100K | 9.13 ± 0.25 | 15.90 ± 1.77 | p = 0.0028 |
Encystation efficiency (% cysts/live cells) | |||
10K | 33.5 ± 4.3 | 81.5 ± 8.9 | p = 0.0011 |
25K | 81.7 ± 17.0 | 86.1 ± 9.0 | NS |
50K | 87.6 ± 5.6 | 83.9 ± 1.4 | NS |
100K | 93.8 ± 2.6 | 86.9 ± 9.7 | NS |
Encystation rate (total cysts/total starting cell population) | |||
10K | 44.0 ± 5.7 | 163.0 ± 17.8 | p = 0.0004 |
25K | 20.2 ± 4.2 | 61.8 ± 6.5 | p = 0.0007 |
50K | 23.3 ± 1.5 | 36.8 ± 0.6 | p = 0.0001 |
100K | 17.6 ± 0.5 | 30.6 ± 3.4 | p = 0.0028 |
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Wesel, J.; Shuman, J.; Bastuzel, I.; Dickerson, J.; Ingram-Smith, C. Encystation of Entamoeba histolytica in Axenic Culture. Microorganisms 2021, 9, 873. https://doi.org/10.3390/microorganisms9040873
Wesel J, Shuman J, Bastuzel I, Dickerson J, Ingram-Smith C. Encystation of Entamoeba histolytica in Axenic Culture. Microorganisms. 2021; 9(4):873. https://doi.org/10.3390/microorganisms9040873
Chicago/Turabian StyleWesel, Jordan, Jennifer Shuman, Irem Bastuzel, Julie Dickerson, and Cheryl Ingram-Smith. 2021. "Encystation of Entamoeba histolytica in Axenic Culture" Microorganisms 9, no. 4: 873. https://doi.org/10.3390/microorganisms9040873