Serological and Molecular Evidence of Bartonella henselae in Stray Cats from Southern Italy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Specimens
2.2. Serological Test
2.3. DNA Extraction and Real-Time Polymerase Chain Reaction (PCR)
2.4. Statistical Analysis
2.5. Ethical Statement
3. Results
3.1. Animal Demographics
3.2. Serological Tests
3.3. Risk Factors Analysis
3.4. Molecular Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | Sequences 5′-3′ | Gene | Amplicons | Reference |
---|---|---|---|---|
B_hen F | TGTCATCAGAAAGGGCTATT | 16S-23S | 183 bp | [26] |
B_hen R | CAAAACAAAGTGCAAAACAA |
Factors | Titer 1:50 | Titer 1:100 |
---|---|---|
Age | ||
<12 months | 16 | 73 |
>12 months | 8 | 106 |
Sex | ||
Female | 14 | 108 |
Male | 10 | 71 |
Coat color | ||
Mixed color | 16 | 131 |
Uniform color | 8 | 48 |
Factors | % pos | N° pos/All | OR (95% CI) |
---|---|---|---|
Age | |||
<12 months | 41.4 | 89/215 | Ref. |
>12 months | 53.3 | 114/214 | 1.593 (1.046–2.426) |
Sex | |||
Female | 46.4 | 122/263 | Ref. |
Male | 48.8 | 81/166 | 1.045 (0.679–1.608) |
Coat color | |||
Mixed color | 49.4 | 147/297 | Ref. |
Uniform color | 42.4 | 56/132 | 0.736 (0.466–1.161) |
Status of Cat | Real-Time PCR Results | ||
---|---|---|---|
Positive (%) | Negative (%) | Total (%) | |
Seropositive | 86 (20) | 117 (27.3) | 203 (47.3) |
Seronegative | 62 (14.5) | 164 (38.2) | 226 (52.7) |
Total | 148 (34.5) | 281 (65.5) | 429 |
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Grippi, F.; Galluzzo, P.; Guercio, A.; Blanda, V.; Santangelo, F.; Sciortino, S.; Vicari, D.; Arcuri, F.; Di Bella, S.; Torina, A. Serological and Molecular Evidence of Bartonella henselae in Stray Cats from Southern Italy. Microorganisms 2021, 9, 979. https://doi.org/10.3390/microorganisms9050979
Grippi F, Galluzzo P, Guercio A, Blanda V, Santangelo F, Sciortino S, Vicari D, Arcuri F, Di Bella S, Torina A. Serological and Molecular Evidence of Bartonella henselae in Stray Cats from Southern Italy. Microorganisms. 2021; 9(5):979. https://doi.org/10.3390/microorganisms9050979
Chicago/Turabian StyleGrippi, Francesca, Paola Galluzzo, Annalisa Guercio, Valeria Blanda, Francesco Santangelo, Sonia Sciortino, Domenico Vicari, Francesca Arcuri, Santina Di Bella, and Alessandra Torina. 2021. "Serological and Molecular Evidence of Bartonella henselae in Stray Cats from Southern Italy" Microorganisms 9, no. 5: 979. https://doi.org/10.3390/microorganisms9050979