A Mouse Model for Studying Post-Acute Arthritis of Chikungunya
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Biosafety
2.3. Virus
2.4. Animal Studies
2.5. Viremia Measurement
2.6. Tarsal Joint Measurement
2.7. Histology
2.8. Histologic Inflammation Scoring System
3. Results
3.1. CHIKV SAH2123 Reliably Causes Infection in Adult C57BL/6J Mice at 48 h Post Infection
3.2. Viremia Resolves by 10–16 DPI
3.3. CHIKV SAH2123 Inoculation Results in Ipsilateral Tarsal Joint Swelling for 10 Days
3.4. Histologic Findings: CHIKV SAH2123 Infection Results in Histologic Evidence of Arthritis, Synovitis, Periostitis and Myositis That Persist to 21 DPI
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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7 DPI | 15 DPI | 21 DPI | 25 DPI | |||||
---|---|---|---|---|---|---|---|---|
Chikungunya inoculated Mice (n = 8) | 10 | 10 | 5 | 5 | 5 | 4 | 0 | 6 |
Non-inoculated Controls (n = 2) | 2 | 0 |
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Chang, A.Y.; Tritsch, S.R.; Porzucek, A.J.; Schwartz, A.M.; Seyler-Schmidt, M.; Glass, A.; Latham, P.S.; Reid, S.P.; Simon, G.L.; Mores, C.N. A Mouse Model for Studying Post-Acute Arthritis of Chikungunya. Microorganisms 2021, 9, 1998. https://doi.org/10.3390/microorganisms9091998
Chang AY, Tritsch SR, Porzucek AJ, Schwartz AM, Seyler-Schmidt M, Glass A, Latham PS, Reid SP, Simon GL, Mores CN. A Mouse Model for Studying Post-Acute Arthritis of Chikungunya. Microorganisms. 2021; 9(9):1998. https://doi.org/10.3390/microorganisms9091998
Chicago/Turabian StyleChang, Aileen Y., Sarah R. Tritsch, Abigail J. Porzucek, Arnold M. Schwartz, Margaux Seyler-Schmidt, Arielle Glass, Patricia S. Latham, St. Patrick Reid, Gary L. Simon, and Christopher N. Mores. 2021. "A Mouse Model for Studying Post-Acute Arthritis of Chikungunya" Microorganisms 9, no. 9: 1998. https://doi.org/10.3390/microorganisms9091998