Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals, Study Design, Housing and Diets
2.2. Data Collection
2.2.1. Blood Sampling
2.2.2. Herd Monitoring
2.3. Serum Analysis
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
Appendix A
Hyperketonemia | Subclinical Ketosis | |||||||
---|---|---|---|---|---|---|---|---|
BHB | NEFA | tBIL | AST | BHB | NEFA | tBIL | AST | |
15–9 days a.p. | - | - | p = 0.02 | - | - | - | p = 0.04 | - |
9–3 days a.p. | p = 0.02 | - | - | - | p = 0.04 | - | - | - |
2–8 days p.p. | p < 0.001 | - | p < 0.01 | - | p < 0.001 | p = 0.02 | p = 0.001 | - |
8–14 days p.p. | p < 0.001 | - | p < 0.001 | - | p < 0.001 | p < 0.01 | p < 0.001 | - |
14–20 days p.p. | p < 0.001 | p < 0.01 | p < 0.001 | p = 0.04 | p < 0.001 | - | p = 0.001 | - |
20–26 days p.p. | p < 0.001 | - | p < 0.01 | - | p < 0.001 | p < 0.001 | p < 0.01 | - |
26–32 days p.p. | p < 0.001 | - | p = 0.01 | - | p < 0.001 | p = 0.01 | p = 0.01 | - |
32–38 days p.p. | p < 0.001 | - | - | - | p < 0.001 | p < 0.001 | p < 0.01 | - |
38–44 days p.p. | p < 0.001 | - | p = 0.02 | - | p = 0.001 | p < 0.01 | p = 0.02 | - |
44–50 days p.p. | p < 0.001 | - | - | - | p < 0.001 | p < 0.001 | p < 0.01 | - |
50–56 days p.p. | p < 0.001 | - | - | - | p < 0.01 | p < 0.001 | p < 0.01 | - |
56–62 days p.p. | p < 0.001 | - | - | - | p < 0.001 | - | p = 0.04 | - |
t0 (day of partum) | p < 0.001 | - | - | - | p < 0.001 | p = 0.04 | p < 0.05 | - |
t10 (10 days p.p.) | p < 0.001 | p < 0.01 | p < 0.001 | - | p < 0.001 | p < 0.01 | p < 0.001 | - |
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Hyperketonemia | SCK | |
---|---|---|
Calculated cut-off value of BHB | 0.54 mmol/L | 0.73 mmol/L |
Se (95% CI) | 0.92 (0.81–0.98) | 0.85 (0.62–0.97) |
Sp (95% CI) | 0.73 (0.58–0.85) | 0.82 (0.71–0.90) |
PPV (95% CI) | 0.80 (0.66–0.94) | 0.55 (0.42–0.87) |
NPV (95% CI) | 0.89 (0.75–0.95) | 0.95 (0.86–0.98) |
FP | 12 | 14 |
FN | 4 | 3 |
TP | 47 | 17 |
TN | 36 | 65 |
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Couperus, A.M.; Schroeder, F.; Hettegger, P.; Huber, J.; Wittek, T.; Peham, J.R. Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers. Animals 2021, 11, 1353. https://doi.org/10.3390/ani11051353
Couperus AM, Schroeder F, Hettegger P, Huber J, Wittek T, Peham JR. Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers. Animals. 2021; 11(5):1353. https://doi.org/10.3390/ani11051353
Chicago/Turabian StyleCouperus, Anna Mareike, Fabian Schroeder, Peter Hettegger, Johann Huber, Thomas Wittek, and Johannes R. Peham. 2021. "Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers" Animals 11, no. 5: 1353. https://doi.org/10.3390/ani11051353