Quercetin Ameliorates Lipopolysaccharide-Induced Duodenal Inflammation through Modulating Autophagy, Programmed Cell Death and Intestinal Mucosal Barrier Function in Chicken Embryos
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Chinese Herbal Medicines with Antidiarrheal Effects and Key Active Ingredients
2.2. Analysis of Potential Targets and Signaling Pathways
2.3. Reagents, Chicken Embryos and Experimental Design
2.4. Histology
2.5. qPCR
2.6. Immunohistochemistry Investigation
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. Bioactive Ingredients and Targets of Chinese Herbal Medicines
3.2. Effects of Quercetin on Intestinal Mucosa after LPS Induction in Chicken Embryos
3.3. Quercetin Ameliorates Gut Inflammation through Modulating Autophagy, Programmed Cell Depth, Barrier Function in Chicken Embryos
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Botanic Name | English Common Name | Name in Chinese Pinyin | Abbreviation |
---|---|---|---|
Folium Artemisiae argyi | Moxa | Aiye | AIYE |
Paeoniae Radix Alba | White peony root | Baishao | BS |
Pulsatilliae Radix | Chinese Pulsatilla root | Baitouweng | BTW |
Platycladi cacumen | Platycladus orientalis leaves | Cebaiye | CBY |
Radix Bupleuri | Chinese thorowax root | Chaihu | CH |
Ailanthi Cortex | Bark of ailanthus | Chunpi | CP |
Radix Sanguisorbae officinalis L. | Garden burnet root | Diyu | DIYU |
Caryophylli flos, Syzygium aromaticum | Clove | Dingxiang | DX |
Glycyrrhizae radix et Rhizoma | Licorice | Gancao | GC |
Alpiniae officinarum Rhizome | Galangal | Gaoliangjiang | GLJ |
Zanthoxylum bungeanum Maxim. Pericarpium | Pepper pericarp | Huajiao | HJ |
Coptidis Rhizoma | Coptis root | Huanglian | HL |
Hedysarum Multijugum Maxim., Astragalus membranaceus | Astragali radix | Huangqi | HQ |
Scutellariae Radix | Baical skullcap root | Huangqin | HQIN |
Lonicerae Japonicae Flos | Honeysuckle flower | Jinyinhua | JYH |
Rosae Laevigatae Fructus | Cherokee rose fruit | Jinyingzi | JYZ |
Portulacae Herba | Purslane | Machixian | MCX |
Chaenomeles sinensis (Thouin) Koehne | Pawpaw | Mugua | MG |
Aucklandiae Radix | Costustoot | Muxiang | MX |
Equiseti Hiemalis Herba | Scouring rush herb | Muzei | MZ |
Pollen Typhae | Cattail pollen | Puhuang | PH |
Panax ginseng C. A. Mey. | Ginseng | Renshen | RS |
Herba Taxilli | Chinese taxillus twig | Sangjisheng | SJS |
Granati Pericarpium | Pomegranate bark | Shiliupi | SLP |
Crataegi folium | Hawthorn leaf | Shanzhaye | SZY |
Mume Fructus | Dark plum fruit | Wumei | WM |
Evodiae fructus | Evodia fruit | Wuzhuyu | WZY |
Leonuri Herba | Motherwort | Yimucao | YMC |
Genes Name | Primer Sequence (5′-3′) | Gene Bank ID |
---|---|---|
ATG5 | F:CCCATCCCTGGTCCGTAAC; R:CGGCGGCGTATACGAAGTA | NM_001006409 |
Bcl-2 | F: TGGCTGCTTTACTCTTGGGG; R:TATCTCGCGGTTGTCGTAGC | NM_205339 |
CASP1 | F:CACTTCCACTTCGGATGGCT; R:CCACGAGACAGTATCAGGCG | XM_015295935 |
CASP3 | F:ACCGAGATACCGGACTGTCA; R:GCCATGGCTTAGCAACACAC | NM_204725 |
CASP12 | F:AATAGTGGGCATCTGGGTCA; R:CGGTGTGATTTAGACCCGTAAGAC | [7] |
Claudin 1 | F:CTGGGTCTGGTTGGTGTGTT; R:CGAGCCACTCTGTTGCCATA | NM_001013611 |
Drp1 | F: GGCAGTCACAGCAGCTAACA; R:GCATCCATGAGATCCAGCTT | NM_001079722 |
Fas | F:GTCAGTGCTGCACGAAATGT; R:AACCTCCAAACCGAGTGCTT | NM_001199487 |
GAPDH | F: GAGAAACCAGCCAAGTATGATG; R: CACAGGAGACAACCTGGTCC | NM_204305 |
IFNγ | F:CTGACAAGTCAAAGCCGCAC; R:CTTCACGCCATCAGGAAGGT | NM_205149 |
IL-10 | F:TGCGAGAAGAGGAGCAAAGC; R:AACTCCCCCATGGCTTTGTAG | AJ621254 |
IL-1β | F:GCTCAACATTGCGCTGTACC; R:AGGCGGTAGAAGATGAAGCG | FJ537850 |
IL-6 | F:ACGAGGAGAAATGCCTGACG; R:CTTCAGATTGGCGAGGAGGG | NM_204628 |
IL-8 | F:TGCCAGTGCATTAGCACTCA; R:TTGGCGTCAGCTTCACATCT | HM179639 |
LC3-1 | F:GCATCCAAACAAAATCCCAGTC; R:AAGCCATCCTCATCCTTCTCCT | XM_040688401 |
LC3-2 | F:CTTCTTCCTCCTGGTGAACG; R:GCACTCCGAAAGTCTCCTGA | NM_001031461 |
LKB1 | F:AGCAGAGGCATTGCATCCAT; R:CCTGCGGACCAGATGTCTAC | NM_001045833 |
MMP9 | F:ACACAGACTCTATGCTGCCTG; R:GAGAGTAGGGCGGGGAAAAT | NM_204667 |
MMP3 | F:ATCAGGCTCTACAGTGGTG; R:ATGGGATACATCAAGGCAC | XM_025152201 |
Mucin 2 | F:TCAGATCAGATGGCAGTGTGTC; R:AATCTGCAGCTGAAGCCCAAA | JX284122 |
MYD88 | F:TTAGTCTTTCCCCAGGGGCT; R:GCCAGTCTTGTCCAGAACCA | NM_001030962 |
NFKB1 | F:TCAACGCAGGACCTAAAGACAT; R:GCAGATAGCCAAGTTCAGGATG | NM_001396396 |
Occludin | F:TACATCATGGGCGTCAACCC; R:CCAGATCTTACTGCGCGTCT | NM_205128 |
p38 | F:GGTCGGTGAGCTGGTAAAGG; R:CGCTTTCAGCTTCTGTCGGA | XM_015296032 |
RIPK1 | F:GATCCATTTGCGAAGCTGCC; R:CTTAGGCTAATGGCGCTGGT | NM_204402 |
TLR4 | F:GGCTCAACCTCACGTTGGTA; R:AGTCCGTTCTGAAATGCCGT | KP410249 |
ZO-1 | F:TATGAAGATCGTGCGCCTCC; R:GAGGTCTGCCATCGTAGCTC | XM_015278977 |
TNFα | F:CCCATCCCTGGTCCGTAAC; R:CGGCGGCGTATACGAAGTA | MF000729 |
Gene ID | Gene Name Abbreviation | Gene Name |
---|---|---|
5243 | ABCB1 | ATP Binding Cassette Subfamily B Member 1) |
135 | ADORA2A | Adenosine A2a Receptor |
196 | AHR | Aryl Hydrocarbon Receptor |
207 | AKT1 | AKT Serine/Threonine Kinase 1 |
240 | ALOX5 | Arachidonate 5-Lipoxygenase |
3577 | CXCR1 | C-X-C Motif Chemokine Receptor 1 |
1956 | EGFR | Epidermal Growth Factor Receptor |
2100 | ESR2 | Estrogen Receptor 2 |
4314 | MMP3 | Matrix Metallopeptidase 3 |
4318 | MMP9 | Matrix Metallopeptidase 9 |
4350 | MPG | N-Methylpurine DNA Glycosylase |
6850 | SYK | Spleen Associated Tyrosine Kinase |
7015 | TERT | Telomerase Reverse Transcriptase |
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Yu, J.; Hu, G.; Cao, H.; Guo, X. Quercetin Ameliorates Lipopolysaccharide-Induced Duodenal Inflammation through Modulating Autophagy, Programmed Cell Death and Intestinal Mucosal Barrier Function in Chicken Embryos. Animals 2022, 12, 3524. https://doi.org/10.3390/ani12243524
Yu J, Hu G, Cao H, Guo X. Quercetin Ameliorates Lipopolysaccharide-Induced Duodenal Inflammation through Modulating Autophagy, Programmed Cell Death and Intestinal Mucosal Barrier Function in Chicken Embryos. Animals. 2022; 12(24):3524. https://doi.org/10.3390/ani12243524
Chicago/Turabian StyleYu, Jinhai, Guoliang Hu, Huabin Cao, and Xiaoquan Guo. 2022. "Quercetin Ameliorates Lipopolysaccharide-Induced Duodenal Inflammation through Modulating Autophagy, Programmed Cell Death and Intestinal Mucosal Barrier Function in Chicken Embryos" Animals 12, no. 24: 3524. https://doi.org/10.3390/ani12243524