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Review

A Review of Toxoplasma gondii in Animals in Greece: A FoodBorne Pathogen of Public Health Importance

by
Isaia Symeonidou
1,†,
Georgios Sioutas
1,†,
Thomai Lazou
2,
Athanasios I. Gelasakis
3 and
Elias Papadopoulos
1,*
1
Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
Laboratory of Hygiene of Foods of Animal Origin—Veterinary Public Health, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
3
Laboratory of Anatomy and Physiology of Farm Animals, Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, 11855 Athens, Greece
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Animals 2023, 13(15), 2530; https://doi.org/10.3390/ani13152530
Submission received: 12 July 2023 / Revised: 31 July 2023 / Accepted: 3 August 2023 / Published: 5 August 2023
Versions Notes

Abstract

:

Simple Summary

Toxoplasma gondii is a parasite that can infect humans and animals, mainly through meat consumption. It is also the second most important pathogen transmitted with food in Europe. However, detecting the presence of T. gondii in animal meat differs on a country basis since there are no mandatory controls along the food chain in the European Union. Underreporting of cases is still a problem in many countries like Greece. The current review examines the prevalence of T. gondii in animals in Greece and identifies the risks associated with meat transmission. Certain animals like sows, wild boars, hares, equines, and cats had lower levels of infection, while sheep and goats generally had higher levels compared to other European countries and to the global averages. The level of infection in chickens was similar between Greece and Europe, while there was high variation in cattle studies, with no data regarding dairy products. Until now, Greece has not implemented a comprehensive system to ensure meat safety, particularly regarding T. gondii. This review highlights the preventive measures that the state should implement to ensure food safety and protect public health, as well as the various control measures that should be adopted by consumers to reduce the infection risk.

Abstract

Toxoplasma gondii is a zoonotic protozoon with a complex life cycle and the second most important foodborne pathogen in Europe. Surveillance of toxoplasmosis is based on national considerations since there are no mandatory controls along the food chain in the European Union, and underreporting of meat is still a problem in many countries like Greece. The current review provides an overview of T. gondii prevalence, associated risk factors, and surveillance in animals in Greece, focusing on the transmission role of meat and highlighting the control measures that should be adopted by consumers. Sows, wild boars, hares, equines, and cats had lower, while sheep and goats generally had higher seroprevalence than their respective pooled European and global values. Seroprevalence in chickens was similar between Greece and Europe, while there was high variation in cattle studies, with no data regarding dairy products. Though a comprehensive meat safety assurance system is the most effective approach to control the principal biological hazards associated with meat, such as T. gondii, the prerequisite risk categorisation of farms and abattoirs based on EFSA’s proposed harmonised epidemiological indicators has not materialised as yet in Greece. Therefore, comprehensive control strategies are still required to ensure food safety and safeguard public health.

1. Introduction

Among foodborne pathogens, parasites have been neglected primarily due to their complex life cycles, prolonged incubation period, several transmission routes, and chronic impact on hosts [1]. However, several zoonotic foodborne parasites (FBP) are today considered emerging threats and have been increasingly recognised as being responsible for considerable disease burdens worldwide [2] based on multicriteria decision analyses and estimations [3]. In this context, the second-highest-ranked FBP in Europe is the zoonotic protozoon Toxoplasma gondii (T. gondii) [4].
This obligate intracellular coccidian parasite has an indirect life cycle, with the sexual reproduction occurring only in the small intestine of Felidae (definitive hosts) and asexual multiplication taking place extra-intestinally into the tissues (tissue cysts) of all warm-blooded animals, including humans (intermediate hosts). There are three different infectious stages: sporozoites in oocysts, tachyzoites, usually in secretions, and bradyzoites in tissue cysts [5]. When hosts get infected, tachyzoites quickly proliferate inside different cells [6]. Consequently, tachyzoites form cysts in different tissues and develop into bradyzoites. These tissue cysts survive throughout the host’s lifetime and can infect any human or animal that consumes them. Thus, the consumption of undercooked or raw meat from infected meat-producing animals may pose a risk to public health [6,7]. Oocysts are shed with the faeces of infected felids, particularly kittens, sporulate and may contaminate food, fresh produce, shellfish, and water leading to human infection following consumption [7,8,9]. In addition, humans acquire T. gondii by ingesting undercooked meat containing viable tissue cysts or unpasteurised milk and dairy products containing tachyzoites [10,11]. Toxoplasmosis is also an occupational disease for hunters, butchers, and slaughterhouse workers who may become infected during evisceration [12,13,14,15,16].
Foodborne transmission is considered the primary mode of human infection with T. gondii. A European multicenter case-control study depicted that 30–63% of T. gondii infections in humans could be attributed to meat consumption, including cured meat and wildlife meat, i.e., deer [12]. In the same frame, available data indicated that foodborne transmission accounts for 40–60% of human toxoplasmosis, and the most commonly implicated food sources are the meat of ruminants and pork, as well as vegetables [11,17]. It should be noted that consumers prefer “ready-to-eat” products and favour meat from animals raised in organic farms, i.e., with access to outdoor grazing. On top of that, the tendency to consume rare or raw meat not previously frozen may also increase the risk of ingesting infective T. gondii tissue cysts [7].
Regarding clinical symptoms, T. gondii is considered the most prevalent parasitic zoonotic infection globally [7,8], leading to various diseases in humans and animals. Approximately one-third of the global human population is estimated to be chronically infected with T. gondii [18]. Acquired human toxoplasmosis is typically subclinical, while mild and unspecific symptoms sometimes occur. Long-term consequences, such as ocular symptoms, may exhibit years later [11]. The neurological form of the disease in humans, cerebral toxoplasmosis, has also been associated with schizophrenia, psychiatric and bipolar disorders, among other conditions [18]. Furthermore, acquired toxoplasmosis can be fatal for immunosuppressed individuals and is ranked as the leading cause of death for this population group [19,20]. In particular, the World Health Organization (WHO) has ranked acquired toxoplasmosis and ascariosis as the parasitic diseases with the largest total number of symptomatic cases and, most interestingly, symptomatic cases that are attributed to contaminated food [21]. In the congenital form of the disease, infected children can develop blindness and mental retardation, and infection can even be fatal for the fetus during pregnancy. Studies have demonstrated that the global estimated incidence of congenital toxoplasmosis in humans is 190,100 new cases per year [19,22]. Both congenital and acquired toxoplasmosis have an elevated public health impact [11,17,21]. Toxoplasmosis is the fourth most common cause of hospitalisation and the third leading cause of death among foodborne diseases [6]. Conclusively, T. gondii is an important foodborne pathogen that ranked high in Europe based on the multicriteria decision analyses (MCDA) methods [3] and disability-adjusted life-years (DALY) estimates for disease [2].
The current review provides a detailed overview of T. gondii prevalence and surveillance in animals in Greece. Moreover, it highlights the central role of different types of meats in T. gondii transmission and the primary prevention strategies and measures to limit foodborne transmission.

2. Surveillance across Europe

In humans, congenital toxoplasmosis is notifiable in many European countries. Screening pregnant women is mandatory in some countries (Belgium, France, Slovakia, Croatia, Italy, Poland, Serbia, and Slovenia), while it remains voluntary in others (Bulgaria, Hungary, Czech Republic, and Germany) [23]. Population-based serosurveillance studies have been reported from Australia, Belgium, Germany, Spain, France, Iceland, the Netherlands, Norway, Portugal, and Sweden [24,25,26,27]. Data from these studies in the Netherlands have demonstrated that toxoplasmosis has one of the highest disease burdens among foodborne diseases [2]. In most European countries, passive surveillance of human clinical cases of both hospitalised and other patients exists, but whether these cases are systematically reported is unclear. Nonetheless, underreporting is still a problem in many countries and is attributed mainly to the lack of concise rules for recording. Therefore, more effort is required to improve the assessment of the disease burden and thus prioritise adequate control measures.
As regards livestock, surveillance of toxoplasmosis is voluntarily implemented on a national level since there are no mandatory controls and no regular official recordings regarding T. gondii along the food chain in the European Union (EU) [11]. Specifically, animal toxoplasmosis is notifiable in 14 European countries, such as Belgium, Czechia, Germany, Finland, France, Iceland, Ireland, Liechtenstein, Poland, Slovenia, Latvia, North Macedonia, Serbia, and the Netherlands [4]. However, the obtained data are considered of rather limited value due to various factors such as small sample sizes, non-harmonised sampling schemes, different diagnostic methods, and lack of animal-related information (e.g., age and rearing system) [4,15]. All of the above render impossible the accurate estimation of the prevalence of T. gondii infections in livestock at the EU level [28,29,30,31]. Passive surveillance, i.e., recording animals with compatible clinical signs, such as abortions in small ruminants, is applied in some countries. No obligatory serological monitoring of incoming animals for slaughter is in place in the EU, and optional active surveillance at the abattoir level is carried out inconsistently by serology and molecular methods. Therefore, it cannot substitute routine inspection and ensure meat safety. It should be noted that post-mortem macroscopic examination (visual inspection) of infected meat is unsuitable for T. gondii detection since tissue cysts are too small (100 μm) to identify without using microscopy [6]. In fact, T. gondii meat detection and identification is applied mainly during certain research projects and outbreak investigations of congenital toxoplasmosis. Therefore, surveillance in the EU is relatively inadequate for meat-producing animals. This gap reinforces the necessity of applying risk-based surveillance systems in livestock based on risk assessment surveys similar to the ones already available in the Netherlands and Italy [11] to prevent human meat-borne infections and reduce the disease burden.

3. Diagnostic Approaches

The European Food Safety Authority (EFSA) BIOHAZ Panel has highlighted a paucity of robust and validated diagnostic tools for T. gondii that can be utilised across different types of foodstuffs, including meat samples [11]. This paucity along the parasite’s heterogeneous life cycle constitutes a serious impediment to source attribution studies.
Many studies assess the seroprevalence of T. gondii [32]. One should keep in mind that each study presents a unique approach toward the seroprevalence estimation of the parasite in various animal species and cannot be directly compared with other studies since a vast number of factors such as different diagnostic methods employed, location, population size, animals’ age, breed, gender, weight, farming system, presence of cats and regional climatic conditions influence the outcome [33,34]. Moreover, serology can only be used to estimate the risk of human infection if a correlation exists between seroprevalence and tissue cysts in meat. This correlation has been studied for the main livestock species in an extensive review, and it was demonstrated that the likelihood of detecting parasites in seropositive animals was highest in pigs (58.8%), followed by chickens (53.4%), sheep and goats (39.4% and 35.0%, respectively), and was lowest in horses (8.8–13.8%) and cattle (3.6%). Therefore, the seroprevalence can be utilised to estimate the public health risk of meat-borne toxoplasmosis only as regards these livestock species, but it is not applicable in the case of cattle and horses, in which similar detection rates of the parasite have been reported between seropositive and seronegative animals [35]. Another disadvantage of serology is that there are serological non-responders, i.e., seronegative animals with tissue cysts. This phenomenon has been reported in pigs (4.9%), sheep and goats (1.8% and 2.0%, respectively), chickens (1.8%), cattle (2.4%), and horses (2.4–32.0%). As a result, a seronegative animal may produce T. gondii-infected meat, and serology cannot be employed for individual carcass control [35].
From a public health perspective, this lack of data on the prevalence of T. gondii tissue cysts in cattle and horses remains a crucial gap as beef is a major meat source in many countries in Europe and horse meat in some, such as France and Italy. In this frame, a quantitative risk assessment for meat-borne toxoplasmosis was performed in the Netherlands, and it revealed that beef (rather than pork or mutton) contributed to 67% of the predicted human cases [36]. Moreover, since beef and horse meat are more frequently consumed undercooked or raw than pork or poultry, usually eaten well-cooked [35], this information is essential to accurately reflect the public health risk involved. Several serological studies have been published in Europe (reviewed by Tenter et al.) [32], and seroprevalence ranked from 4% to 92% in sheep, 2% to 92% in cattle, 4% to 77% in goats, 0% to 64% in pigs, and 0 to 53% in horses depending on the husbandry system [37]. Relatively high seroprevalence rates have been observed in sheep and goats in Mediterranean countries, thus pinpointing mutton as an essential meat source of T. gondii infection for consumers [37].

4. Studies on T. gondii in Animals and Their Products in Greece

Different cross-sectional studies, mainly serological ones, and case reports are available on T. gondii in both domestic and wild animals in Greece, namely in domestic swine and wild boars [38,39,40], sheep [41,42,43,44,45,46,47,48,49,50], goats [41,42,43,45,46,47,48,51], cattle [52,53,54], birds [55,56,57], hares [58], equines [59], cats [60,61,62], wildcats [63], and in one camel [64] (Table 1). Some of these animal species, i.e., cats, chickens, or even hares, can be used as sentinels for human infection in specific regions, and their seropositivity can prove helpful in assessing the environmental contamination with oocysts [7,14,57,60]. The prevalence rates discovered in the examined research are compared with worldwide and EU prevalence rates obtained by systematic reviews and meta-analysis studies.

4.1. Pigs and Wild Boars

The occurrence of T. gondii in pigs has been addressed in two studies in Greece. Compared to the worldwide pooled seropositivity in sows (19%) as well as the average European seropositivity (13%) [33], sows in Greece exhibited lower seropositivity (4.3% and 4.4%) [38,39]. Generally, pork originating from organic farms is more frequently infected than conventional farms [65]. In Greece, sows in mountainous areas and farms with low biosecurity measures had a significantly higher risk of infection than those in lowland areas and farms with high biosecurity measures [39]. In addition, sows not vaccinated against porcine circovirus 2 had higher seropositivity rates. This association most likely resulted from inadequate practices in farms with unvaccinated sows, increasing the infection risk with T. gondii [38]. There is only one relevant study conducted on wild boars in Greece, which documented a seroprevalence of 5.2%, a rate also lower than the global pooled seropositivity (23%) and the respective European one (26%) in wild boars [7,13]. Possible risk factors for wild boars include dense populations of boars and confined geographic regions [40].
Nonetheless, swine seroprevalence is not always correlated to the existence of T. gondii bradyzoite tissue cysts in pork meat [6]. Similarly, detecting antibodies or cysts in slaughterhouse samples does not indicate human infection risk due to storing and processing procedures of pork meat after slaughter that can destroy T. gondii cysts [7]. As is the case in Greece, in many countries, the routine cooking of pork combined with the low seroprevalence has significantly reduced the risk of pig-to-human transmission of T. gondii [32]. In this context, a meta-analysis assessing the risk of different foods in human toxoplasmosis did not regard consuming undercooked or raw pork as a significant risk factor [66]. Despite this risk reduction, in the USA, pork meat is still considered a significant threat to human T. gondii infection [67]. Consumers should know that pigs’ brains, lungs, hearts, and tongues are the most commonly infected organs with T. gondii and have a higher parasitic burden than other organs [68,69,70].

4.2. Sheep and Meat Thereof

Toxoplasma gondii seroprevalence in Greek sheep has been extensively studied throughout the years [41,42,43,44,45,46,47,48,49,50]. Estimations ranged from 23% in Crete in 1995 [48] to 90% in Trikala in 2019 [44], with most studies finding seropositivity of around 50% and an upward trend throughout the years, as seen in Table 1. Compared to the global pooled seroprevalence in sheep at 33.86% and the pooled European seroprevalence at 41.01% [71], it becomes evident that Greece has higher seroprevalence and is endemic for ovine toxoplasmosis.
Furthermore, toxoplasmosis can be one of the leading abortion causes in sheep. The seropositivity of T. gondii in abortive sheep has been calculated at 52.1% [46], 60.9% [43], and 49.8% [47] in different studies in Greece. These seropositivity rates are similar to the global pooled seropositivity of T. gondii in abortive sheep calculated at 56% [34]. In one case report, 60% of pregnant ewes aborted, and T. gondii was diagnosed as the causative agent [50]. However, other studies demonstrated no association between T. gondii seroprevalence and abortions in ewes [41,43]. This discrepancy among studies is expected, considering that abortion rates are typically much higher in naïve ewes that acquire the infection for the first time during pregnancy. In contrast, in sheep exposed to T. gondii before pregnancy, immunocompetence establishes, and abortion rates are usually much lower [34,50].
Common risk factors for ovine toxoplasmosis include a herd larger than 300 sheep, most likely increasing infection risk due to overcrowding and increased exposure to infection sources [45]. Sheep co-grazing with other herds may also have an increased probability of infection because it is associated with poor biosecurity measures [45]. Sheep reared under intensive or semi-intensive management schemes, offered feed concentrate, and water from public systems might have a higher risk of infection [41]. The elevated infection risk in these groups may be attributed to a higher stocking density in intensive systems and more intermediate hosts, such as rodents in feed warehouses, compared to extensive systems, while the “water from public systems” might be a confounder [41]. In regards to climatic conditions, high temperatures, low rainfalls, and low altitudes were also associated with increased T. gondii seropositivity in one study, probably deriving from oocyst susceptibility to different weather conditions [45]. In contrast, another study found no difference in ovine seroprevalence between coastal and mountainous regions [41]. Higher seroprevalence has also been documented in sheep living in urban and agricultural/forest areas compared to savanna areas, possibly associated with the presence of infected client-owned or stray cats that excrete oocysts in those former areas [45]. Ewes had a significantly higher seroprevalence than male sheep in one study [43], most likely because male sheep have a more robust immune response against the parasite due to hormonal differences [72,73,74]. This finding agrees with a recent meta-analysis on sheep T. gondii seroprevalence and associated risk factors [71]. Regarding rams, sexual transmission is possible, and an experimental study demonstrated that T. gondii reduces the quality parameters of sperm (viability, motility, velocity), while sulphadimidine treatment did not revert the sperm cell morphological abnormalities [75]. Concerning age, sheep older than four years had higher seroprevalence than younger sheep, most likely due to increased exposure to T. gondii and not because old sheep are more susceptible [7,43,71,76]. It is worth noting that cats were present in all farms in one of these studies and had free access to the feedstuff [43]. As definitive hosts, cats can be a source of infection by expelling oocysts with their faeces on the sheep’s grazing pasture or feed [32,42,71]. However, the presence of cats on the farm level is not always a significant risk factor [41].
Sheep meat is one of the most commonly infected foods regarding human toxoplasmosis [32]. A meta-analysis investigating the prevalence of T. gondii in meat from different animals identified sheep meat as the most infected animal meat, with a global mean prevalence of 14.7%, even surpassing pork (12.3%) [65]. Another meta-analysis classified the consumption of sheep meat as the most crucial risk factor for human foodborne infection with T. gondii [66]. Given the high seroprevalence of T. gondii in sheep in Greece and its growing trend, awareness should be raised in farmers to employ strict biosecurity measures based on risk factor analysis to prevent their animals from infection. Towards this end, screening methods for T. gondii, both at the farm and slaughterhouse level, should be put in effect, and consumers should practice standard hygiene measures and cook mutton thoroughly to reduce infection risk, particularly in the case of immunocompromised individuals [77].

4.3. Goats and Meat Thereof

The seropositivity of T. gondii in goats has been researched alongside sheep seroprevalence, displaying fluctuation through the years and estimated at 14% (1995) [48], 50.4% (2002) [46], 17.9% (2007) [43], 30.7% (2012) [41], 16.8% (2013) [45], and 61.3% (2013) [42]. Comparatively, the worldwide pooled seroprevalence of T. gondii in goats is in the middle at 31.7% and the European value at 38.8%, exhibiting high heterogenicity among the tested regions [71]. In Greece, three studies found that sheep had a significantly higher seroprevalence than goats [41,43,48], but one study reached the opposite conclusion [42]. At a global level, sheep have a higher seroprevalence than goats, but the difference is not statistically significant [71]. Sheep have a higher infection risk than goats due to feeding habits; goats are browsers and feed from plant leaves high from the ground, while sheep are grazers and feed from the vegetation on the ground that is more commonly contaminated with T. gondii oocysts [42]. Although there is a difference between the two animal species, in Greece, both sheep and goats are kept indoors and fed similar food during the colder months. This practice reduces discrepancies in their feeding habits and equalizes the infection risk [42]. However, it is important to note that further research or in-depth studies may be necessary to fully understand the situation in Greek small ruminants. Sheep might additionally have a genetic predisposition to toxoplasmosis compared to goats [41].
In goats, transplacental transmission leading to abortion can also occur if the mother has been infected before pregnancy when tissue cysts get re-activated, which is uncommon in sheep that typically have more robust immune protection [34]. Consequently, goats may abort in multiple breeding periods, which is relatively rare in sheep that usually only abort once [7]. In different studies, the seroprevalence of T. gondii in abortive goats was calculated at 47.9% (2002) [46], 14.3% (2007) [43], and 29.9% (2009) [47]. However, T. gondii seropositivity is not always significantly different between abortive and non-abortive goats [41,43]. Compared to Greece, the global pooled seroprevalence of T. gondii in abortive goats is higher, estimated at 50% [34], indicating that T. gondii is present as a cause of reproductive failure in Greek goats but not as common as in other countries. Nevertheless, this parasite can cause massive abortions in goats, and in a reported case of natural toxoplasmosis in three dairy goat herds, abortion rates reached as high as 78.5% without treatment [51].
Concerning risk factors, goats in intensive or semi-intensive farms and those in large herds (>300 animals) have a higher infection risk [41,45]. Goats are more crowded in intensive farms, and large herds are in closer contact with infection sources like young cats and their faeces [41,45,47]. In like manner, providing goats feed concentrate may increase infection risk because cats with free access to the feed can expel oocysts with their faeces and contaminate them [41]. Furthermore, seropositivity in goats increases with age, like in sheep, because goats have antibodies for many years after they come in contact with the parasite, and as they age, their chances of exposure to T. gondii increase [43]. Weather conditions such as high temperatures, low rainfalls, and farm altitude can also potentially increase infection risk due to increased oocyst survival in these climates [45].
In goat meat, T. gondii predilection sites include the lungs, brain, and dorsal muscles, which also have a higher parasitic burden than other tissues [78]. Regardless of the tissue location, cysts have an unequal disposition in goats’ meat [7], which may present a higher risk of transmitting the parasite to humans than other meats like pork [39].

4.4. Cattle and Meat Thereof

Cattle are generally poor hosts to T. gondii, resistant to disease, and have low seropositivity compared to small ruminants [79]. Laboratory studies have demonstrated that although cattle can acquire the parasite just as easily as small ruminants, T. gondii does not survive long in their tissues, and the number of cysts decreases close to zero within a few days [80]. Consequently, cattle become seronegative after a while, so seroprevalence studies cannot be used as an indication for cattle harbouring cysts with bradyzoites [81]. In Greece, three previous studies calculated cattle seropositivity at 39.7% (1992) [53], 20.0% (2005) [54], and 8.1% (2020) [52], indicating that the pathogen is present in cattle farms, irrespective of the farm management system [54]. However, none of the three studies examined possible cross-reactivity between Toxoplasma spp., Neospora spp., and possibly other cyst-forming coccidia (e.g., Besnoitia spp., Sarcocystis spp.) that are relevant for cattle [82]. Despite this, a decline in seroprevalence rate was observed through the years, just like in the rest of the world [83], although each study was conducted in a different region. Still, the seroprevalence of T. gondii in Greek cattle was higher in two studies than the global pooled seroprevalence in cattle at 16.9% [83]. Nonetheless, reproductive failures and calf mortality due to T. gondii infections are very rare in cattle [34,54], with Neospora caninum infections being much more common [34].
No specific risk factors for cattle toxoplasmosis in Greece have been investigated before, but in two studies, adult cattle had higher seropositivity than young ones, suggesting that the risk of infection increases as cattle get older [52,54]. Again, this increase can be attributed to increased exposure to T. gondii infection sources, like oocysts in the environment [52].
Consuming raw or undercooked beef has been established as a significant risk factor for human toxoplasmosis [34,66,84]. This finding contradicts the knowledge that cattle are typically considered unsuitable hosts for T. gondii, and beef is rarely infected with the protozoon [66]. Nonetheless, consumers’ habits of eating beef, typically raw or undercooked, compared with other types of meats that are preferred cooked (i.e., pork) have led to beef being a significant infection source for human toxoplasmosis [66]. The impact of beef on human toxoplasmosis is also enhanced by the large quantities of beef consumed each year compared to other types of meat [85], as well as by the fact that beef constitutes a major meat source in several European nations. In fact, as mentioned earlier, in one study in the Netherlands, beef caused more than 67% of T. gondii meat-borne infections [36].

4.5. Birds and Poultry Products

Birds have three prominent roles in the epidemiology of T. gondii. Firstly, they are common prey for cats in urban and rural environments; secondly, domestic (i.e., chickens) or wild birds (i.e., game meat) are consumed by humans, and lastly, birds can cover large distances transporting T. gondii to new previously uncontaminated locations [55].
As regards seropositivity in Greek birds, in the single study conducted in chickens, the overall seroprevalence of T. gondii was 9.4%, with backyard chickens exhibiting much higher seropositivity at 41.2%, layers at 2.8%, while all broilers were negative [57]. Only one broiler chicken was positive after a PCR examination [57]. These findings align with other studies on T. gondii in chickens that showed a high seroprevalence in backyard chickens, a low seroprevalence in layers/caged chickens, and an almost zero seroprevalence in broilers [7]. In another study on domestic and urban (wild) pigeons in Northern Greece, seropositivity using ELISA was 5.8% and 0%, respectively [56], although a small number of wild pigeons was examined (n = 50). When examining woodcock populations from two different areas of the country with PCR, 4.7% of woodcocks were positive for T. gondii, indicating that wild birds harbour the parasite in Greece, too [55].
Significant risk factors for infection included chickens with outdoor access, meaning they grazed freely, and those that fed without an automatic feeder [57]. An explanation could be that free-range chickens eat transport hosts (i.e., earthworms) that mechanically carry T. gondii oocysts [57]. This result is consistent with other studies revealing a lower seroprevalence of T. gondii in caged chickens [7]. Surprisingly, the presence of cats was not a significant risk factor, indicating that chickens may not acquire the infection directly through the faeces of infected cats but through other sources [57]. There were no significant differences in seroprevalence among the regions examined [57]. Seroprevalence studies in chickens could benefit the food industry since chickens remain seropositive for a long time, and also, there is a good association between the existence of antibodies and the presence of T. gondii DNA in chickens [57]. Additionally, a strong positive correlation exists between antibody titers and parasitic burden in chickens [7].
In a similar pattern, retail chicken meat can be PCR positive for T. gondii, with the prevalence reaching up to 10% in some countries [86,87], even surpassing beef and pork in one case [88]. However, in some countries, chicken meat is typically sold frozen in retail stores, a practice that kills T. gondii tissue cysts [7]. In regards to other poultry products and T. gondii, consuming raw eggs was not considered a significant risk factor for foodborne human toxoplasmosis in a meta-analysis, despite a few old studies describing the detection of T. gondii in eggs [7,66].

4.6. Hares and Meat Thereof

There is only one available study that detected a 5.7% seroprevalence of T. gondii in hares in Greece [58]. This seroprevalence was on the lower end of T. gondii seropositivity in hares from other studies and countries, ranging from 0–21% [14]. Interestingly, no liver samples were positive for the protozoon with PCR [58], like in some other studies investigating the presence of T. gondii in different hare tissues [14]. Despite these results, PCR has successfully detected the parasite in some infected hares; thus, T. gondii tissue cysts could be present in the meat of seropositive hares [14]. Regardless of the low seroprevalence in Greece, the demand for hare meat is on the rise, posing a risk, especially for hunters that consume raw/undercooked game meat [7,14]. Rainfalls and the type of land hares lived on (i.e., forests and grasslands) were assessed as significant risk factors for T. gondii infection due to increased oocyst survivability and dense populations of wildcats/transport hosts, respectively [58].

4.7. Equines and Meat Thereof

In one study conducted on 770 equines (753 horses, 13 mules, and 7 ponies) carried out by Kouam et al. in 2010, the seroprevalence of IgG against T. gondii was 1.8% [59]. This seropositivity rate was relatively low compared to the global pooled equine seroprevalence at 11.3% [89]. Equines used in farms and equines in Thessaly and Peloponnese had a significantly higher seroprevalence than those used for racing or recreation and equines living in Attica, respectively [59]. Horses in farming probably acquired the parasite by ingesting oocysts from the environment (contaminated water or feed) or through the accidental ingestion of infected meat or offal [59]. Despite the low seroprevalence, T. gondii has been detected in more than 50% of horse meat using mouse bioassays in two surveys in Egypt and Brazil [65]. Consumption of horse meat is extremely rare in Greece and, thus, is not regarded as a vital source of human infection [59]. However, monitoring equine infection is necessary due to possible adulteration cases of beef with horse meat, like in the 2013 European scandal [90].

4.8. Cats

Felines are the only definitive hosts of T. gondii and excrete oocysts with their faeces [60]. However, cats typically shed oocysts 6–10 days after primary infection [91] and 4 days after re-infection [92]. Therefore only 0.4% of domestic cats shed oocysts whenever faeces are collected [93], and the prevalence of 0% [61] and 0.4% [62] of T. gondii-like oocysts in the faeces of cats examined in two previous studies in Greece are unremarkable. Concerning wild felids, 2.4% of them expel oocysts at any given moment globally [93], and this is in concordance with the prevalence of T. gondii-like oocysts in the faeces of wildcats in Greece (1.6% and 4.3%) [63]. It should be kept in mind that when conducting faecal studies for T. gondii, oocysts need to be further identified with molecular methods (i.e., PCR) because the feline coccidia Hammondia hammondi, Besnoitia spp., and T. gondii all share the same morphology and cannot be differentiated with microscopy [60,63].
The countrywide seroprevalence of T. gondii in cats in Greece was recently estimated to be 21.8% [60], which is lower compared to both the global pooled seroprevalence at 37.5% and the European seroprevalence at 45.3% [94]. Cats in the geographical region of Peloponnese displayed the highest seropositivity at 42.8% and, interestingly, previous studies also detected high T. gondii seroprevalence in sheep [43] and equines [59] in this specific area, indicating a potentially high environmental oocyst burden in Peloponnese [60].
Risk factor analyses identified cats in rural areas, cats that hunted, and cats with outdoor access as having a significantly higher probability of infection [60]. Rodents, the protozoon’s natural secondary host, and other small animals, birds, and transport hosts can harbour T. gondii, and cats that hunt can get infected when eating them [60]. Similarly, cats with outdoor access can hunt more often, and cats in rural areas come in contact with more intermediate hosts and oocysts in the environment [60]. In the binary logistic regression model, hunting in urban areas remained the only significant risk factor, indicating that most cats in Greece acquire the infection by ingesting bradyzoites in tissue cysts, the natural transmission of T. gondii for cats [60,95].

4.9. Dairy Products

Consumption of raw milk is also considered a risk factor and a possible foodborne transmission route for tachyzoites, which are the parasite stage likely to be shed in the milk of infected animals during lactation [11,96]. Although drinking milk is generally not a significant risk factor for T. gondii infection [66], explicitly drinking unpasteurised goat milk is [84]. Indeed, human toxoplasmosis cases have been attributed to the consumption of raw milk from infected goats [11,97]. Pasteurisation and low pH values are generally regarded as sufficient to inactivate tachyzoites. However, it has been evidenced that the latter survived for at least 60 minutes in gastric fluids mixed with various quantities of artificially spiked cow’s milk samples due to gastric pH value fluctuations [7,96]. In European countries, the prevalence of T. gondii in raw milk samples via PCR assays has been reported to range from 4% to 11% as regards sheep milk samples [98,99,100] and from 4% to 65% with reference to goat milk samples [99,101,102] whereas 16% of cow milk samples were found positive in one study [99]. However, data are scarce regarding the occurrence of T. gondii in raw milk from Greek farms. In an experimental study in Greece, T. gondii was detected in ovine and caprine milk samples until 28 days post-infection (p.i.) [103]. As in the case of raw milk, the consumption of related unpasteurised dairy products (e.g., whey, fresh cheese) represents another route for transmission to consumers, and T. gondii has been detected in cheese originating from unpasteurised goat milk [104]. In contrast, cheese from sheep milk is deemed safe for human consumption, probably due to the cheese-making process (pH, salt concentration) that deactivates any T. gondii in the milk [77].

5. Control Measures for Foodborne Toxoplasmosis

As stated before, T. gondii-relevant data in current food chain information are limited, and current meat inspection practices lack effectiveness in reducing human toxoplasmosis health risks attributed to meat consumption [29,30,31,105]. In addition, cross-contamination is a key aspect of HACCP and food safety management systems for controlling foodborne bacteria in the meat production chain but not an issue of concern in the case of intracellular parasites, such as T. gondii.
Given the above, consumers are responsible for employing appropriate food-handling practices [65]. Water deactivates tissue cysts, and thus, after preparing meat, hands should be properly cleansed with soapy water [7]. Kitchen utensils, knives, cutting boards, and countertops used to prepare meat should be washed with hot water and soap and cleaned frequently to avoid cross-contamination of food products [66]. Moreover, it is recommended that people only drink pasteurised milk (especially if it is from goats) and treated-filtered water. In particular, lake or river water should first be boiled [106]. It is worth mentioning that meat consumption per se or eating meat frequently is not associated with a higher risk of T. gondii infection [66]. Generally, consuming raw/undercooked [66] cured, smoked, locally produced, or age-dried meat [84] is unsafe and can significantly increase the risk of infection with T. gondii. Similarly, tasting the meat while it is being cooked should be avoided, unlike tasting seasonings, which are considered safe [66]. Mollusks and other sea animals should also be cooked before consumption [107].
Several options are available for killing tissue cysts containing bradyzoites in infected meat, and they are separated into three large categories: freezing, cooking, and alternative methods [108,109,110]. Freezing infected meat can kill tissue cysts if the temperature reaches −20 °C for 3 days minimum, while lower temperatures can inactivate cysts even quicker [6,109,110]. Heating is the most commonly used method for destroying T. gondii tissue cysts in meat, provided that the internal meat temperature is at least 67 °C for around a minute [6,110]. Consumers should be aware that the thickness and kind of the meat cut will affect the time required to kill tissue cysts [7]. Using a sheet of aluminium foil can aid in the even spread of heat across the meat [6]. Some alternative methods that have proven effective include meat irradiation at 75–100 krad and high-hydrostatic pressure processing at 340–400 MPa for at least 1 minute, but their high price and alteration of meat organoleptic characteristics (texture, colour) make them less preferable options [6,109,111,112].
On the other hand, microwaving is ineffective in destroying T. gondii tissue cysts in meat [6] due to asymmetrical heating [108]. In like manner, salting or smoking are unreliable methods for deactivating T. gondii tissue cysts in meat such as pork [32] since some tissue cysts may survive curing [6]. Furthermore, the time needed for bradyzoite destruction by curing meat is far longer than cooking or freezing, with some studies showing a curing period of at least 1 year, depending on the physicochemical parameters used [113]. Chilling meat also does not affect the viability of T. gondii tissue cysts [109]. In summary, freezing and cooking are the safest and most effective methods, while no standard concentrations and times are 100% effective in killing tissue cysts to advocate adopting and using these last methods [7].

6. Risk-based Control of Meat-borne Toxoplasmosis

Over ten years ago, EFSA received a mandate from the European Commission to evaluate meat inspection in a public health context aiming at the science-based modernisation of the process. Thus, EFSA performed a risk ranking of the principal biological hazards associated with meat from all domestic meat-producing animals based on the incidence and severity of human meat-borne diseases and proposed a comprehensive meat safety assurance system (MSAS) as the most effective approach to control the main hazards in the context of meat inspection [28,29,30,31,105,114,115]. This risk-based MSAS (RB-MSAS) focuses on high-risk (high-priority) public health hazards for which a meat safety risk reduction is envisaged by combining a range of preventive measures and controls applied both on the farm (pre-harvest phase) and at the abattoir (harvest phase) in a longitudinally integrated way. To this end, EFSA proposed harmonised epidemiological indicators (HEIs) for the risk categorisation of farms (presence of priority hazards in animal batches intended for slaughter) and abattoirs (capacity of reducing the relevant risk and setting appropriate targets for final chilled carcasses). Within this context, an epidemiological indicator is defined as “the prevalence or incidence of the hazard at a certain stage of the food chain or an indirect measure of the hazards that correlates to human health risk caused by the hazard”. The T. gondii public health relevance and proposed HEIs in livestock and poultry in the EU, according to EFSA, are summarised in Table 2.
Regarding classification in terms of priority, congenital or acquired toxoplasmosis incidence and severity are quite different (the first is rare but severe in contrast to the latter). However, due to available data indicating a high attribution of toxoplasmosis to the meat of pigs, small ruminants, and farmed boar and deer, T. gondii has been classified as a priority hazard in these animal species [31,105,114]. On the contrary, it could not be classified in terms of priority (undetermined) in the case of cattle and solipeds [30,115] due to the absence of robust epidemiological associations between their meat and toxoplasmosis, which induces high uncertainty in the relevant risk assessment.
As recently reviewed by Blagojevic et al. [116], the RB-MSAS in the EU, as proposed by EFSA, has not been practically materialised in its entity due to various practical challenges that remain to be resolved. For example, HEIs have not been introduced in a formalised way in any of the 18 European countries, including Greece, that participated in a recent study investigating the risk categorisation of abattoirs [117]. Moreover, no farm as yet has acquired the status of ‘controlled housing conditions’ in Greece, irrespective of the livestock species bred, though such a status is a component of the T. gondii-specific HEIs (Table 2). However, the high public health relevance of T. gondii in sheep and goat meat is particularly important from a Greek perspective, given the large number of small ruminants reared in the country and the various culinary traditions related to the consumption of their meat (carcasses and offal).
Table 2. Toxoplasma gondii public health relevance and proposed harmonised epidemiological indicators (HEIs) in the EU [28,29,30,31,105,114,115,118,119,120,121].
Table 2. Toxoplasma gondii public health relevance and proposed harmonised epidemiological indicators (HEIs) in the EU [28,29,30,31,105,114,115,118,119,120,121].
Animal SpeciesPublic Health RelevanceIndicators (Animal/Food Category/Other)Food Chain StageAnalytical/Diagnostic MethodSpecimen
Sheep and GoatsHighHEI 1: Farms with controlled husbandry conditionsFarmAuditingNot applicable
HEI 2: Information on the age of the animalsAbattoirFood chain informationNot applicable
HEI 3: Detection of T. gondii infectionAbattoirSerologyBlood
HEI 4: Detection of T. gondii infection in older animals (more than one year) from farms with controlled husbandry conditionsAbattoirSerologyBlood
HEI 5: Absence of T. gondii infection in younger animals (less than one year) from farms without controlled husbandry conditionsAbattoirSerologyBlood
Farmed deer and farmed wild boarHighHEI 1: Detection of T. gondii antibodies in all farmed deer and wild boarAbattoirSerologyMeat juice
HEI 2: Detection of T. gondii antibodies in the older animals (over one year) of farmed deer and wild boarAbattoirSerologyMeat juice
SwineMediumHEI 1: Farms with officially recognised controlled housing conditions (including control of cats and boots)FarmAuditingNot applicable
HEI 2: T. gondii in breeding pigs from officially recognised controlled housing conditionsAbattoirSerologyBlood
HEI 3: T. gondii in all pigs from non-officially recognised controlled housing conditionsAbattoirSerologyBlood
Poultry (Broilers)LowNot applicableNot applicableNot applicableNot applicable
BovineUndeterminedNot applicableNot applicableNot applicableNot applicable

7. Conclusions

In Greece, sows, wild boars, hares, horses, and cats typically had lower seropositivity for T. gondii than the pooled European and worldwide values, but sheep and goats usually had greater seroprevalence compared to the European average values. No data were recorded on dairy products. There was substantial variation in studies of cattle and similar seroprevalence in chickens across Greece and Europe. Factors like feeding habits, housing conditions, and genetic predisposition influence infection risk among the different animal species, and control measures from consumers, such as freezing and thorough cooking of meat, are still necessary to reduce infection risk. Without any national mandatory screening of farm animals for antibodies and of carcasses for T. gondii tissue cysts, the infection risk for consumers is not under control. As regards meat-borne toxoplasmosis within the Greek context, the high public health relevance of T. gondii in sheep and goat meat (as per EFSA) is of particular interest. To this end, national initiatives towards the implementation of preventive measures and controls applied both on the small ruminant farms (pre-harvest phase) and at the abattoirs (harvest phase) in a longitudinally integrated way are necessary to effectively protect public health.

Author Contributions

Conceptualization, I.S., A.I.G. and E.P.; methodology, I.S., G.S., T.L., A.I.G. and E.P.; software, G.S. and A.I.G.; validation, I.S., G.S., T.L., A.I.G. and E.P.; formal analysis, I.S., G.S. and T.L.; investigation, I.S., G.S. and T.L.; resources, I.S., G.S. and E.P.; data curation, I.S., G.S., T.L., A.I.G. and E.P.; writing—original draft preparation, I.S., G.S. and T.L.; writing—review and editing, I.S., G.S., T.L., A.I.G. and E.P.; visualisation, I.S., G.S. and E.P.; supervision, E.P.; project administration, E.P.; funding acquisition, I.S., G.S. and E.P. All authors have read and agreed to the published version of the manuscript.

Funding

This review received no external funding.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

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Table 1. Studies on Toxoplasma gondii in animals in Greece.
Table 1. Studies on Toxoplasma gondii in animals in Greece.
A/AAnimal SpeciesNumber of Animals TestedYear of PublicationLocationType of StudyDiagnostic MethodResults and Remarks
1Pigs609 sows (65 farms)2016 [39]Mainland GreeceSeroprevalenceIFAT and ELISA4.3% (26/609).
Risk factors: Farms in mountainous areas and farms with low biosecurity measures
2Pigs364 sows2021 [38]Not specifiedSeroprevalenceIFAT4.4% (16/364).
Seropositive sows had higher AST and CK activity
Risk factors: sows not vaccinated against porcine circovirus
3Wild boars94 wild boars2015 [40]Different areas of GreeceSeroprevalenceIFAT5.2% (5/94)
4Sheep and Goats8700 sheep
2320 goats		1995 [48]CreteSeroprevalenceELISASheep: 23% (2001/8700)
Goats: 14% (325/2320)
Sheep had significantly higher seroprevalence than goats
5Sheep840 examined by IFAT
450 examined by ELISA		2001 [49]Mainland GreeceSeroprevalenceIFAT and ELISAIFAT: 53.4% (449/840)
ELISA: 58.5% (263/450)
All farms were conventional (non-organic)
6Sheep and Goats250 sheep (25 farms)
250 goats (26 farms)		2002 [46]Southern Greece, IslandsSeroprevalenceIFATSheep: 47.6% (119/250)
Seroprevalence in abortive sheep: 52.1% (86/165)
Goats: 50.4% (126/250)
Seroprevalence in abortive goats:47.9% (69/144)
7Sheep and Goats182 sheep (9 farms)
167 goats (6 farms)		2007 [43]Peloponnese, western Central Greece, and IoanninaSeroprevalenceELISASheep: 50.5% (92/182)
Seroprevalence in abortive sheep: 60.9% (14/23)
Goats: 17.9% (30/167)
Seroprevalence in abortive goats:
14.3% (7/49)
All farms were organic
Sheep had significantly higher seroprevalence than goats
Sheep risk factors: Female sex, increased age
Goat risk factors: Increased age
8Sheep and Goats289 sheep (37 farms)
174 goats (18 farms)		2009 [47]Southern GreeceSeroprevalenceIFATSeroprevalence in abortive sheep: 49.8% (144/289)
Seroprevalence in abortive goats:
29.9% (52/174)
Sheep farms were semi-extensiveGoat farms were extensive
9Sheep500 sheep (1 farm)2011 [50]Northern GreeceCase ReportELISA and histopathology60% (300/500) of the sheep had aborted due to T. gondii
10Sheep and Goats1501 sheep (60 farms)
541 goats (41 farms)		2012 [41]Northern Greece (Thessaloniki, Chalkidiki, Kastoria)SeroprevalenceELISASheep: 48.6% (729/1501)
Goats: 30.7% (166/541)
No regional differences were found; sheep had significantly higher seroprevalence than goats
Risk factors for both animal species: intensive or semi-intensive farming, feeding concentrate, water from public supply
11Sheep and Goats360 sheep (34 farms)
179 goats
(20 farms)		2013 [45]ThessalySeroprevalenceELISASheep: 28.3% (102/360)
Goats: 16.8% (30/179)
Risk factors for both animal species: herd size, anthelmintic treatment, class of anthelmintic, grazing with other flocks, farmer education, farm altitude, and generalised land cover
12Sheep and Goats458 sheep (50 farms)
375 goats (50 farms)		2013 [42]Different areas of GreeceSeroprevalenceELISASheep: 53.7% (246/458).
Goats: 61.3% (230/375)
Goats had significantly higher seroprevalence than sheep
13Goats920 goats (3 farms)2013 [51]Northern GreeceCase ReportPCR, histopathology, serologyThe abortion rate without treatment ranged from 11% to 78.5%
14Sheep80 sheep2019 [44]Trikala, Asimenio-Didimotiho, Xilokeriza-Corinthia, Velestino-Volos, Giannitsa, Sitihori-Didimotiho, Loutraki-Corinthia, Aliveri-EviaSeroprevalenceMAT56.25% (45/80). Risk factors: Geographic region, sheep from Trikala, Asimenio-Didimotiho, Xilokeriza-Corinthia Velestino-Volos, and Giannitsa, had significantly higher seroprevalence than sheep from Loutraki-Corinthia
15Cattle1890 cattle1992 [53]SerresSeroprevalenceComplement fixation test39.7% (751/1890)
16Cattle105 cattle2005 [54]ThessalonikiSeroprevalenceELISA20% (21/105)
well-managed intensive farms, Friesian cattle
17Cattle627 cattle(7 farms)2020 [52]ThessalySeroprevalenceELISA8.1% (51/627)
All farms had previous reproductive problems, and cats present
18Pigeons379 domestic pigeons
50 wild pigeons		2011 [56]Northern GreeceSeroprevalenceELISADomestic pigeons 5.8% (22/379)
Wild pigeons 0% (0/50)
19Woodcock86 woodcocks2017 [55]Macedonia,
Mesolonghi		Molecular prevalencePCR4.7% (4/86)
20Chickens934 chickens
(8 broiler farms, 14 backyard farms, 20 layer farms)		2022 [57]Epirus, Central Macedonia, Central Greece-AtticaSeroprevalenceELISA9.4% (88/934)
Risk factors: Farming system, nutrition type, and automatic feeding
21Hares105 hares2019 [58]Northern and Central GreeceSeroprevalenceIFAT5.7% (6/105)
No positive liver sample with PCR
Risk factors: Precipitation indices and land uses
22Equines753 horses
13 mules
7 ponies		2010 [59]Peloponnese, Attica, Thessaly, MacedoniaSeroprevalenceELISA1.8% (14/773)
Risk factors: Activity type, location
23Cats1150 cats2018 [61]CountrywideFaecal prevalenceSedimentation and Flotation technique0% (0/1150)
24Cats264 cats2017 [62]CreteFaecal prevalenceSedimentation and Flotation technique0.4% (1/264)
Oocysts were T. gondii-like, not confirmed with PCR
25Cats1554 cats2022 [60]CountrywideSeroprevalenceImmunochromatographic test21.8% (339/1554)
Risk factors: hunting, rural areas, outdoor access
26Wildcats23 wildcat carcasses
62 faecal samples		2021 [63]Different areas of GreeceFaecal prevalenceSedimentation and Flotation techniqueFaecal samples 1.6% (1/62)
Faeces of necropsied animals 4.3% (1/23)
Oocysts were T. gondii-like, not confirmed with PCR
27Camel1 Camel[64]TrikalaCase reportELISA, PCR, cytologyThe female camel was pregnant with a high antibody titer against T. gondii and aborted. The aborted foetus was positive for tissue cysts in brain smears and positive in PCR for T. gondii
Abbreviations: Aspartate aminotransferase (AST), creatine kinase (CK), enzyme-linked immunoassay (ELISA), indirect fluorescence antibody test (IFAT), modified agglutination test (MAT), polymerase chain reaction (PCR).
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MDPI and ACS Style

Symeonidou, I.; Sioutas, G.; Lazou, T.; Gelasakis, A.I.; Papadopoulos, E. A Review of Toxoplasma gondii in Animals in Greece: A FoodBorne Pathogen of Public Health Importance. Animals 2023, 13, 2530. https://doi.org/10.3390/ani13152530

AMA Style

Symeonidou I, Sioutas G, Lazou T, Gelasakis AI, Papadopoulos E. A Review of Toxoplasma gondii in Animals in Greece: A FoodBorne Pathogen of Public Health Importance. Animals. 2023; 13(15):2530. https://doi.org/10.3390/ani13152530

Chicago/Turabian Style

Symeonidou, Isaia, Georgios Sioutas, Thomai Lazou, Athanasios I. Gelasakis, and Elias Papadopoulos. 2023. "A Review of Toxoplasma gondii in Animals in Greece: A FoodBorne Pathogen of Public Health Importance" Animals 13, no. 15: 2530. https://doi.org/10.3390/ani13152530

APA Style

Symeonidou, I., Sioutas, G., Lazou, T., Gelasakis, A. I., & Papadopoulos, E. (2023). A Review of Toxoplasma gondii in Animals in Greece: A FoodBorne Pathogen of Public Health Importance. Animals, 13(15), 2530. https://doi.org/10.3390/ani13152530

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