The Distribution of IgT mRNA+ Cells in the Gut of the Atlantic Salmon (Salmo salar L.)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Study
2.2. Sampling
2.3. In Situ Hybridization
2.4. Image Acquisition and mRNA+ Cell Counting
2.5. Statistical Analyses
2.6. Ethical Statement
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group 1 (avg. 153 g) | Group 2 (avg. 1717 g) | ||
---|---|---|---|
Unvaccinated | Vaccinated | Unvaccinated | Vaccinated |
(3 fish) | (3 fish) | (3 fish) | (3 fish) |
Probe | Accession No. | Target Region (bp) | Catalog No. | |
---|---|---|---|---|
Target | IgT | GQ907003 | 3–883 | 532171 |
pIgR | GQ892057.1 | 119–1145 | 845451 | |
Control | dapB (negative) | EF191515 | 414–862 | 310043 |
ppib (positive) | NM_001140870 | 20–934 | 494421 |
Probe | Section | Group 1 | Group 2 | ||
---|---|---|---|---|---|
Unvaccinated | Vaccinated | Unvaccinated | Vaccinated | ||
IgT | Pc | − | − | − | + |
FsMi | − | − | − | + | |
SsMi | −/+ | −/+ | −/+ | ++ | |
Ps | − | − | − | + | |
pIgR | SsMi | + | −/+ | + | −/+ |
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Castro, P.L.; Barac, F.; Hansen, T.J.; Fjelldal, P.G.; Hordvik, I.; Bjørgen, H.; Koppang, E.O. The Distribution of IgT mRNA+ Cells in the Gut of the Atlantic Salmon (Salmo salar L.). Animals 2023, 13, 3191. https://doi.org/10.3390/ani13203191
Castro PL, Barac F, Hansen TJ, Fjelldal PG, Hordvik I, Bjørgen H, Koppang EO. The Distribution of IgT mRNA+ Cells in the Gut of the Atlantic Salmon (Salmo salar L.). Animals. 2023; 13(20):3191. https://doi.org/10.3390/ani13203191
Chicago/Turabian StyleCastro, Pedro Luis, Fran Barac, Tom Johnny Hansen, Per Gunnar Fjelldal, Ivar Hordvik, Håvard Bjørgen, and Erling Olaf Koppang. 2023. "The Distribution of IgT mRNA+ Cells in the Gut of the Atlantic Salmon (Salmo salar L.)" Animals 13, no. 20: 3191. https://doi.org/10.3390/ani13203191