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Open AccessArticle
Analyzing the Interaction between Tetrahymena pyriformis and Bacteria Under Different Physicochemical Conditions When Infecting Guppy Using the eDNA Method
by
Jialu Wang
Jialu Wang ,
Xiaosong Wang
Xiaosong Wang ,
Lihui Liu
Lihui Liu ,
Xiang Wang
Xiang Wang ,
Jiarui Wang
Jiarui Wang ,
Yue Zheng
Yue Zheng ,
Li Wang
Li Wang and
Xuming Pan
Xuming Pan *
Laboratory of Protozoology, Harbin Normal University, Harbin 150025, China
*
Author to whom correspondence should be addressed.
Animals 2024, 14(15), 2194; https://doi.org/10.3390/ani14152194 (registering DOI)
Submission received: 7 June 2024
/
Revised: 9 July 2024
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Accepted: 25 July 2024
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Published: 27 July 2024
Simple Summary
Simple Summary: In this study, environmental DNA technology, 16S rRNA gene sequencing, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were employed to investigate the relationships between Tetrahymena pyriformis, bacteria, and guppies under varying temperature and pH conditions. The abundance of T. pyriformis in water, the relative abundance of bacterial species, and histopathological observations were studied. Results of this study showed that several bacteria were related to T. pyriformis, and environmental factors affected the interaction between bacteria and T. pyriformis.
Abstract
In the aquaculture system of ornamental fish, the interaction between bacterial microbiota and ciliate protozoa can prevent or promote disease outbreaks, and different physicochemical conditions will affect the relationships between them. We investigated the interaction between bacterial microbiota and the parasite Tetrahymena pyriformis when infecting Poecilia reticulata (guppy) under different physicochemical conditions. The abundance of T. pyriformis in water, the relative abundance of bacterial species, and histopathological observation were studied or monitored using environmental DNA (eDNA) extraction technology, the qPCR method, and 16s rRNA sequencing, respectively. The morphological identification and phylogenetic analysis of T. pyriformis were carried out. The infected guppy tissue was also stained by the hematoxylin and eosin methods. The results showed: (1) the bacterial communities of water samples were mainly composed of species assigned to Proteobacteria and Bacteroidetes, and Tabrizicola and Puniceicoccaceae were positively correlated with fish mortality, T. pyriformis abundance, and temperature. (2) Arcicella and Methyloversatilis universalis with different correlations between ciliates appeared in different treatment groups, the result of which proved that environmental factors affected the interaction between bacteria and T. pyriformis. (3) Lower temperatures and a higher pH were more beneficial for preventing disease outbreaks.
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MDPI and ACS Style
Wang, J.; Wang, X.; Liu, L.; Wang, X.; Wang, J.; Zheng, Y.; Wang, L.; Pan, X.
Analyzing the Interaction between Tetrahymena pyriformis and Bacteria Under Different Physicochemical Conditions When Infecting Guppy Using the eDNA Method. Animals 2024, 14, 2194.
https://doi.org/10.3390/ani14152194
AMA Style
Wang J, Wang X, Liu L, Wang X, Wang J, Zheng Y, Wang L, Pan X.
Analyzing the Interaction between Tetrahymena pyriformis and Bacteria Under Different Physicochemical Conditions When Infecting Guppy Using the eDNA Method. Animals. 2024; 14(15):2194.
https://doi.org/10.3390/ani14152194
Chicago/Turabian Style
Wang, Jialu, Xiaosong Wang, Lihui Liu, Xiang Wang, Jiarui Wang, Yue Zheng, Li Wang, and Xuming Pan.
2024. "Analyzing the Interaction between Tetrahymena pyriformis and Bacteria Under Different Physicochemical Conditions When Infecting Guppy Using the eDNA Method" Animals 14, no. 15: 2194.
https://doi.org/10.3390/ani14152194
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