Enzymic Activity, Metabolites, and Hematological Responses in High-Risk Newly Received Calves for “Clinical Health” Reference Intervals
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Location Where the Study Was Performed
2.2. Ethics Statement
2.3. Inclusion Criteria
2.4. Cattle Processing
2.5. Feeding and Health Management
2.6. Assessment of Enzymic Activity, Metabolites, and Hematological Responses
2.7. Calculation of Reference Intervals
3. Results and Discussion
3.1. Enzymic Activity
3.2. Metabolites
3.2.1. Total Protein, Blood Urea Nitrogen, and Creatinine
3.2.2. Total Bilirubin, Total Cholesterol, and Triglycerides
3.2.3. Calcium
3.2.4. Glucose
3.2.5. Electrolytes
3.3. Hematological Responses
3.3.1. White Blood Cells
3.3.2. Platelets
3.3.3. Red Blood Cells
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Item 2 | Descriptive Statistics | 95% Reference Intervals (RIs) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Mean | SD 3 | CV 4 | Median | Min | Max | Lower Limit of RIs (90% CI) 5 | Upper Limit of RIs (90% CI) | |||
ALP, UI | 209.70 | 107.00 | 51.03 | 197.00 | 14.00 | 562.00 | 14.40 | 14.00–57.00 | 469.60 | 427.00–539.00 |
GGT, UI | 17.00 | 8.90 | 52.35 | 14.00 | 10.00 | 62.00 | 10.00 | 10.00–10.00 | 47.90 | 33.00–61.00 |
AST, UI | 68.80 | 21.90 | 31.83 | 65.00 | 32.00 | 168.00 | 38.90 | 37.30–40.60 | 124.20 | 115.50–133.00 |
ALT, UI | 26.50 | 7.30 | 27.55 | 25.00 | 16.00 | 65.00 | 16.60 | 16.20–17.20 | 44.40 | 42.00–47.20 |
TP, g/dL | 6.22 | 0.83 | 13.34 | 6.30 | 4.00 | 8.00 | 4.40 | 4.18–4.61 | 7.71 | 7.59–7.83 |
ALB, g/dL | 2.97 | 0.50 | 16.84 | 3.00 | 1.50 | 4.40 | 1.90 | 1.60–2.10 | 3.70 | 3.70–4.30 |
GLO, g/dL | 3.18 | 0.50 | 15.72 | 3.20 | 2.00 | 4.30 | 2.20 | 2.10–2.30 | 4.11 | 4.10–4.30 |
ALB/GLO ratio | 0.94 | 0.17 | 18.09 | 0.92 | 0.58 | 1.65 | 0.68 | 0.64–0.70 | 1.32 | 1.25–1.53 |
BUN, mg/dL | 11.08 | 2.31 | 20.85 | 10.80 | 6.30 | 17.40 | 6.91 | 6.50–7.20 | 16.10 | 15.60–17.10 |
CRE, mg/dL | 0.81 | 0.20 | 24.69 | 0.78 | 0.48 | 1.42 | 0.52 | 0.49–0.55 | 1.35 | 0.49–1.41 |
TBIL, mg/dL | 0.34 | 0.29 | 85.29 | 0.20 | 0.12 | 2.00 | 0.20 | 0.20–0.20 | 1.30 | 1.10–1.80 |
TCHO, mg/dL | 78.60 | 22.00 | 27.99 | 75.00 | 50.00 | 148.00 | 50.00 | 50.00–50.00 | 127.70 | 122.00–129.00 |
TG, mg/dL | 17.00 | 6.90 | 40.59 | 16.00 | 10.00 | 36.00 | 10.00 | 10.00–10.00 | 33.00 | 31.00–35.00 |
Ca, mg/dL | 10.28 | 1.42 | 13.81 | 10.50 | 6.60 | 13.40 | 7.12 | 6.54–7.38 | 12.50 | 12.54–12.88 |
GLU, mg/dL | 89.00 | 22.50 | 25.28 | 92.00 | 20.00 | 146.00 | 26.10 | 20.00–39.00 | 126.00 | 121.00–140.00 |
Na+, mEq/L | 126.30 | 12.10 | 9.58 | 128.00 | 89.00 | 149.00 | 98.20 | 94.00–104.00 | 143.00 | 141.00–149.00 |
K+, mEq/L | 4.93 | 1.21 | 24.54 | 4.70 | 2.80 | 9.20 | 3.11 | 3.00–3.40 | 8.59 | 7.90–8.80 |
Cl−, mEq/L | 90.80 | 9.80 | 10.79 | 91.00 | 70.00 | 119.00 | 71.10 | 70.00–74.00 | 109.00 | 107.00–115.00 |
Item 1 | Current Research 2 | Means of Different Publications 3 | ||
---|---|---|---|---|
Rodríguez-Cordero et al. [21] | Crawford et al. [17] | Smock et al. [19] | ||
ALP, UI | 14.40–469.60 (209.70 ± 107.00) | 379.40 ± 37.33 *** | - | 111.36 ± 7.81 *** |
GGT, UI | 10.00–47.90 (17.00 ± 8.90) | 16.60 ± 2.06 | - | - |
AST, UI | 38.90–124.20 (68.80 ± 21.90) | 74.70 ± 7.61 *** | - | 69.38 ± 3.20 |
ALT, UI | 16.6–44.4 (26.50 ± 7.30) | - | 16.92 ± 1.39 *** | 19.55 ± 0.76 *** |
TP, g/dL | 4.40–7.71 (6.22 ± 0.83) | 5.90 ± 0.22 *** | - | 6.96 ± 0.09 *** |
ALB, g/dL | 1.90–3.70 (2.97 ± 0.50) | 3.90 ± 0.50 *** | - | 2.45 ± 0.05 *** |
GLO, g/dL | 2.20–4.11 (3.18 ± 0.50) | 3.10 ± 0.20 * | - | 4.50 ± 0.10 *** |
BUN, mg/dL | 6.91–16.10 (11.08 ± 2.31) | 11.30 ± 0.49 | 8.67 ± 0.54 *** | 8.22 ± 0.30 *** |
CRE, mg/dL | 0.52–1.35 (0.81 ± 0.20) | 0.75 ± 0.028 *** | 1.20 ± 0.07 *** | 1.19 ± 0.04 *** |
TBIL, mg/dL | 0.20–1.30 (0.34 ± 0.29) | 0.23 ± 0.018 *** | - | 0.29 ± 0.008 ** |
TCHO, mg/dL | 50.00–127.70 (78.60 ± 22.00) | 65.50 ± 4.02 | - | - |
TG, mg/dL | 10.00–33.00 (17.00 ± 6.90) | 35.10 ± 5.97 *** | - | - |
Ca, mg/dL | 7.12–12.50 (10.28 ± 1.42) | 10.10 ± 1.18 * | - | 10.27 ± 0.09 |
GLU, mg/dL | 26.10–126.00 (89.00 ± 22.50) | 106.40 ± 7.31 *** | 90.17 ± 3.38 | 94.74 ± 3.06 *** |
Na+, mEq/L | 98.20–143.00 (126.30 ± 12.10) | 121.20 ± 1.90 *** | 140.75 ± 0.72 *** | 135.82 ± 0.31 *** |
K+, mEq/L | 3.11–8.59 (4.93 ± 1.21) | 4.4 ± 0.10 *** | 5.70 ± 0.13 *** | 5.20 ± 0.07 *** |
Cl−, mEq/L | 71.10–109.00 (90.80 ± 9.80) | 85.7 ± 1.65 *** | 96.88 ± 0.69 *** | 94.81 ± 0.28 *** |
Item 2 | Descriptive Statistics | 95% Reference Intervals (RIs) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Mean | SD 3 | CV 4 | Median | Min | Max | Lower limit of RIs (90% CI) 5 | Upper limit of RIs (90% CI) | |||
WBC, ×103 cells/μL | 9.65 | 2.62 | 27.15 | 9.70 | 3.50 | 15.70 | 4.60 | 4.20–5.20 | 15.20 | 14.20–15.60 |
LYM, ×103 cells/μL | 5.87 | 1.60 | 27.26 | 5.90 | 1.60 | 9.80 | 2.60 | 2.10–3.10 | 9.00 | 8.60–9.50 |
LYM, % | 59.20 | 9.48 | 16.01 | 59.90 | 13.20 | 84.50 | 33.60 | 27.30–41.70 | 74.61 | 72.90–84.50 |
MON, ×103 cells/μL | 0.83 | 0.26 | 31.33 | 0.80 | 0.20 | 1.40 | 0.30 | 0.20–0.40 | 1.40 | 1.30–1.40 |
MON, % | 8.28 | 1.50 | 18.12 | 8.10 | 3.50 | 13.50 | 5.54 | 5.40–6.40 | 12.00 | 11.10–13.10 |
GRA, ×103 cells/μL | 3.33 | 1.42 | 42.64 | 3.10 | 0.20 | 7.90 | 1.10 | 0.70–1.50 | 6.74 | 6.00–7.10 |
GRA, % | 35.30 | 14.82 | 41.98 | 32.10 | 10.10 | 86.80 | 17.51 | 14.70–20.30 | 85.57 | 80.30–86.30 |
PLT, ×103 cells/μL | 239.80 | 90.70 | 37.82 | 224.00 | 69.00 | 470.00 | 91.20 | 70.00–107.00 | 444.90 | 414.00–470.00 |
MPV, fL | 7.22 | 0.97 | 13.43 | 7.10 | 6.00 | 15.20 | 6.14 | 6.10–6.20 | 9.08 | 8.30–10.20 |
RBC, ×106 cells/μL | 9.77 | 1.07 | 10.95 | 9.75 | 4.97 | 12.42 | 7.88 | 7.37–8.08 | 11.90 | 11.56–12.20 |
RDW, % | 26.40 | 2.54 | 9.62 | 26.80 | 13.70 | 31.30 | 19.11 | 17.70–20.20 | 30.13 | 29.30–30.70 |
HGB, g/100 mL | 12.05 | 1.31 | 10.87 | 12.00 | 7.20 | 15.70 | 9.40 | 9.10–9.90 | 14.40 | 14.10–15.20 |
HCT, % | 34.27 | 3.92 | 11.44 | 34.30 | 17.50 | 49.90 | 26.65 | 25.80–28.70 | 41.93 | 40.90–43.40 |
MCV, fL | 35.12 | 3.10 | 8.83 | 34.80 | 27.50 | 44.90 | 29.10 | 27.50–29.90 | 41.80 | 40.30–42.80 |
MCH, pg | 12.36 | 1.03 | 8.33 | 12.40 | 10.50 | 15.70 | 10.60 | 10.60–10.80 | 14.40 | 14.10–15.00 |
MCHC, g/dL | 35.35 | 1.98 | 5.60 | 35.50 | 29.00 | 41.50 | 30.58 | 30.00–31.10 | 38.93 | 38.30–42.00 |
Item 1 | Current Research 2 | Means of Different Publications 3 | |||
---|---|---|---|---|---|
Motta et al. [20] | Rodríguez-Cordero et al. [21] | Scott et al. [18] | Smock et al. [19] | ||
WBC, ×103 cells/μL | 4.60–15.20 (9.65 ± 2.62) | - | 8.90 ± 0.54 *** | 7.32 ± 1.29 *** | 13.03 ± 0.72 *** |
LYM, ×103 cells/μL | 2.60–9.00 (5.87 ± 1.60) | - | 5.10 ± 0.31 *** | - | 6.79 ± 0.53 *** |
LYM, % | 33.60–74.61 (59.20 ± 9.48) | - | 54.00 ± 1.77 *** | 51.63 ± 11.92 *** | 53.17 ± 1.19 *** |
MON, ×103 cells/μL | 0.30–1.40 (0.83 ± 0.26) | - | 0.70 ± 0.06 *** | - | 1.70 ± 0.05 *** |
MON, % | 5.54–12.00 (8.28 ± 1.50) | - | 7.90 ± 0.34 | 8.36 ± 4.5 | 13.43 ± 1.15 *** |
GRA, ×103 cells/μL | 1.10–6.74 (3.33 ± 1.42) | - | 3.10 ± 0.27 | - | - |
GRA, % | 17.51–85.57 (35.30 ± 14.82) | - | 38.00 ± 1.70 ** | - | - |
PLT, ×103 cells/μL | 91.20–444.90 (239.80 ± 90.70) | 263.81 ± 120.09 *** | 243.00 ± 21.08 | - | 513.70 ± 33.84 *** |
MPV, fL | 6.14–9.08 (7.22 ± 0.97) | - | 7.2 ± 0.25 | - | - |
RBC, ×106 cells/μL | 7.88–11.90 (9.77 ± 1.07) | 9.14 ± 1.02 *** | 10.10 ± 0.25 *** | 9.03 ± 0.68 *** | 8.97 ± 0.35 *** |
RDW, % | 19.11–30.13 (26.40 ± 2.54) | - | 26.00 ± 0.48 | 27.56 ± 3.02 * | - |
HGB, g/100 ml | 9.40–14.40 (12.05 ± 1.31) | 11.26 ± 0.99 * | 11.60 ± 0.25 * | 12.49 ± 0.91 *** | 12.37 ± 0.36 *** |
HCT, % | 26.65–41.93 (34.27 ± 3.92) | 33.35 ± 2.92 * | 34.30 ± 0.70 | 35.0 ± 2.53 ** | 36.08 ± 0.95 *** |
MCV, fL | 29.10–41.80 (35.12 ± 3.10) | 36.68 ± 2.79 *** | 34.10 ± 0.78 *** | 38.84 ± 2.85 *** | 40.39 ± 0.64 *** |
MCH, pg | 10.60–14.40 (12.36 ± 1.03) | 12.37 ± 0.88 | 11.50 ± 0.26 *** | 13.85 ± 0.81 *** | 13.86 ± 0.62 *** |
MCHC, g/dL | 30.58–38.93 (35.35 ± 1.98) | 33.74 ± 0.89 *** | 34.00 ± 0.64 *** | 35.69 ± 0.98 * | 34.38 ± 1.27 *** |
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Carrillo-Muro, O.; Rodríguez-Cordero, D.; Hernández-Briano, P.; Correa-Aguado, P.I.; Medina-Flores, C.A.; Huerta-López, L.A.; Rodríguez-Valdez, F.J.; Rivera-Villegas, A.; Plascencia, A. Enzymic Activity, Metabolites, and Hematological Responses in High-Risk Newly Received Calves for “Clinical Health” Reference Intervals. Animals 2024, 14, 2342. https://doi.org/10.3390/ani14162342
Carrillo-Muro O, Rodríguez-Cordero D, Hernández-Briano P, Correa-Aguado PI, Medina-Flores CA, Huerta-López LA, Rodríguez-Valdez FJ, Rivera-Villegas A, Plascencia A. Enzymic Activity, Metabolites, and Hematological Responses in High-Risk Newly Received Calves for “Clinical Health” Reference Intervals. Animals. 2024; 14(16):2342. https://doi.org/10.3390/ani14162342
Chicago/Turabian StyleCarrillo-Muro, Octavio, Daniel Rodríguez-Cordero, Pedro Hernández-Briano, Paola Isaira Correa-Aguado, Carlos Aurelio Medina-Flores, Luis Arturo Huerta-López, Francisco Javier Rodríguez-Valdez, Alejandro Rivera-Villegas, and Alejandro Plascencia. 2024. "Enzymic Activity, Metabolites, and Hematological Responses in High-Risk Newly Received Calves for “Clinical Health” Reference Intervals" Animals 14, no. 16: 2342. https://doi.org/10.3390/ani14162342