Association between IGF-1 and IGFBPs in Blood and Follicular Fluid in Dairy Cows Under Field Conditions
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Study Design and Sample Collection
2.3. Laboratory Analysis
2.3.1. Blood Beta Hydroxybutyrate (BHB)
2.3.2. IGF-1
2.3.3. IGFBP
2.3.4. Gene Expression
Gene | Accession Number | Primer Sequence | Literature | |
---|---|---|---|---|
GAPDH | NM_ 001034034.1 | Forward | CAA CAT CAA GTG GGG TGA TG | [45] |
Reverse | GGC ATT GCT GAC AAT CTT GA | |||
18S rRNA | NR_ 036642.1 | Forward | ACC CAT TCG AAC GTC TGC CCT ATT | [39] |
Reverse | TCC TGG GAT GTG GTA GCC GTT TCT | |||
IGFBP-2 | AF074854 | Forward | GAC GGG AAC GTG AAC TTG ATG | [41] |
Reverse | TCC TTC ATG CCG GAC TTG A | |||
IGFBP-4 | NM_ 174557.3 | Forward | CCC AAG TCT GTG GGA GAA GA | [45] |
Reverse | AAG GAC CTG GGG AGG AGT AA | |||
PAPP-A | XM_ 613511.6 | Forward | TGG AGA ACG CTT CGC TCA ACT G | [46] |
Reverse | ACG CTG GGT CCT GTC TGG CTT T | |||
IGF1R | XM_ 606794.3 | Forward | CCA AAA CCG AAG CTG AGA AG | [45] |
Reverse | TCC GGG TCT GTG ATG TTG TA |
2.4. Statistical Analysis
3. Results
3.1. IGF-1
3.2. IGFBP
3.3. Gene Expression
4. Discussion
4.1. IGF-1 in Plasma and Follicular Fluid
4.2. IGFBP in Plasma and Follicular Fluid
4.3. Association between Metabolism and IGF-1/IGFBP
4.4. Gene Expression in the Follicle
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Molar Mass | Concentration of Stock Solution | Final Concentration | Amount Used | |
---|---|---|---|---|
Ammonium chloride (NH4Cl) | 53.49 g/Mol | 0.15 mM/L | 8.023 g | |
Sodium hydrogen carbonate (NaHCO3) | 84.01 g/Mol | 10 mM/L | 0.840 g | |
Disodium EDTA (Na2EDTA) | 250 mM/L | 0.1 mM/L | 0.4 mL | |
Distilled, Autoclaved, and filtered H2O a | add 1 L |
Follicle Size (mm) | RNA (ng/µL) |
---|---|
18 | 3.5 |
18 | 5.4 |
12 a | 41.9 a |
9 a | 52.6 a |
18 | 78.1 |
20 b | 16.1 b |
9 b | 116.6 b |
12 | 207.7 |
Concentration Used Solution | Used Volume (µL) | Final Concentration | |
---|---|---|---|
RNA | 20 ng/µL | 10 | 200 ng |
First Strand Buffer | 5× | 4 | 1× |
dNTP | 10 mM | 1.5 | 187.5 nmol |
Random Primer | 25 ng/µL | 1.5 | 37.5 ng |
H2O | 3 | ||
Total | 20 |
Concentration Used Solution | Used Volume (µL) | Final Concentration | |
---|---|---|---|
First Strand Buffer | 5× | 1 | 1× |
M-MLV Reverse transcriptase | 200 U/µL | 1 | 200 U |
H2O | 3 | ||
Total | 5 |
Parameter | Study Part | Time Point (Days Postpartum) | n | p-Value |
---|---|---|---|---|
BHB | ||||
in serum | I | 30 ± 2 | 78 | 0.183 |
II | 32 ± 3 | 25 | ||
I | 50 ± 3 | 77 | 0.5542 | |
II | 53 ± 3 | 25 | ||
I | 65 ± 16 | 17 | 0.0004 | |
II | 32 ± 3 | 25 | ||
IGF-1 | I | 30 ± 2 | 78 | 0.8186 |
in plasma | II | 32 ± 3 | 25 | |
IGF-1 | I | 65 ± 16 | 17 | <0.0001 |
in FF | II | 32 ± 3 | 25 |
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Sample Type | Parameter | Samples | |||
---|---|---|---|---|---|
Study Part | |||||
I | II | ||||
n | Time Point | n | Time Point | ||
blood | |||||
BHB | 78 | 30 ± 2 | x | ||
77 | 50 ± 3 | 25 | 53 ± 3 | ||
17 | 65 ± 16 | 25 | 32 ± 3 | ||
IGF-1 | 78 | 30 ± 2 | x | ||
77 | 50 ± 3 | x | |||
17 | 65 ± 16 | 25 | 32 ± 3 | ||
IGFBP | 17 | 65 ± 16 | x | ||
follicular fluid a | |||||
IGF-1 | 27 | 65 ± 16 | 25 | 32 ± 3 | |
IGFBP | 27 | 65 ± 16 | x | ||
granulosa cells | |||||
Gene expression | 6 | 65 ± 16 | x |
IGFBP-Total (ng/mL) | IGFBP-2 (ng/mL) | IGFBP-3 (ng/mL) | IGFBP-4 (ng/mL) | IGFBP-5 (ng/mL) | IGFBP Fragment (ng/mL) | |
---|---|---|---|---|---|---|
Plasma (n = 17) | 5411 a ± 2467 | 1707 a,c,g ± 904.7 | 2312 a ± 895.7 | 175.5 b,d,f/1061/54.0 | 545.3 b,c /1667/233 | 280.9 d ± 175.7 |
FF (n = 27) | 4385 a ± 2356 | 362.7 b,e/2512/16.4 | 1956 a,g ± 797.8 | 191.8 d,f /2937/23.5 | 863.9 b,g ± 362.4 | 406.8 b,d ± 149.4 |
Follicle Size (in mm) | IGFBP-2 | IGFBP-4 | PAPP-A | IGF-1 Receptor | 18SrRNA and GAPDH | ||||
---|---|---|---|---|---|---|---|---|---|
CtIGFBP-2 | ΔCt * | CtIGFBP-4 | ΔCt * | CtPAPP-A | ΔCt * | CtIGF-1 Receptor | ΔCt * | CtRG | |
12 1 | 30.63 1 | 15.06 1 | 33.29 1 | 17.72 1 | 31.04 1 | 15.47 1 | 5.08 1 | 10.81 1 | 15.58 1 |
9 1 | 33.23 1 | 14.961 | 30.74 1 | 12.47 1 | 28.45 1 | 10.18 1 | 5.66 1 | 7.94 1 | 18.28 1 |
18 | 31.69 | 13.60 | 34.01 | 15.92 | 30.14 | 12.05 | 8.60 | 9.96 | 18.10 |
20 2 | 32.56 2 | 11.83 2 | 34.27 2 | 13.54 2 | 38.97 2 | 18.24 2 | 9.56 2 | 15.93 2 | 20.73 2 |
9 2 | 29.07 2 | 12.76 2 | 28.04 2 | 11.73 2 | 27.84 2 | 11.53 2 | 5.54 2 | 7.73 2 | 16.31 2 |
12 | 30.47 | 14.39 | 28.22 | 12.14 | 26.63 | 10.55 | 5.89 | 10.55 | 16.08 |
mean | 31.28 a | 13.76 b | 31.43 a | 13.92 b | 30.51 a | 13.00 b | 6.72 a | 10.48 b | 17.51 |
SD | 0.62 | 0.52 | 1.16 | 0.98 | 1.81 | 1.30 | 0.76 | 1.21 | 0.79 |
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Schiffers, C.; Serbetci, I.; Mense, K.; Kassens, A.; Grothmann, H.; Sommer, M.; Hoeflich, C.; Hoeflich, A.; Bollwein, H.; Schmicke, M. Association between IGF-1 and IGFBPs in Blood and Follicular Fluid in Dairy Cows Under Field Conditions. Animals 2024, 14, 2370. https://doi.org/10.3390/ani14162370
Schiffers C, Serbetci I, Mense K, Kassens A, Grothmann H, Sommer M, Hoeflich C, Hoeflich A, Bollwein H, Schmicke M. Association between IGF-1 and IGFBPs in Blood and Follicular Fluid in Dairy Cows Under Field Conditions. Animals. 2024; 14(16):2370. https://doi.org/10.3390/ani14162370
Chicago/Turabian StyleSchiffers, Christina, Idil Serbetci, Kirsten Mense, Ana Kassens, Hanna Grothmann, Matthias Sommer, Christine Hoeflich, Andreas Hoeflich, Heinrich Bollwein, and Marion Schmicke. 2024. "Association between IGF-1 and IGFBPs in Blood and Follicular Fluid in Dairy Cows Under Field Conditions" Animals 14, no. 16: 2370. https://doi.org/10.3390/ani14162370