Enteropathogenic and Multidrug-Resistant blaCTX-M-Carrying E. coli Isolates from Dogs and Cats
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Processing and Bacteria Identification
2.2. Antimicrobial Susceptibility Testing and Phenotypic Characterization of Extended-Spectrum Beta-Lactamase
2.3. DNA Extraction and Bacterial Genotypic Analysis
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Function-Related Group | Target Gene | Primer Sequences | Amplicon Size (bp) | Reference |
---|---|---|---|---|
Antimicrobial resistance | blaCTX-M | 5′-CGCTTTGCGATGTGCAG-3′ | 550 | [18] |
5′-ACCCGCGATATCGTTGGT-3′ | ||||
Phylogroup | chuA | 5′-ATGGTACCGGACGAACCAAC-3′ | 288 | [14] |
5′-TGCCGCCAGTACCAAAGACA-3′ | ||||
yjaA | 5′-CAAACGTGAAGTGTCAGGAG-3′ | 211 | [14] | |
5′-AATGCGTTCCTCAACCTGTG-3′ | ||||
TspE4.C2 | 5′-CACTATTCGTAAGGTCATCC-3′ | 152 | [14] | |
5′-AGTTTATCGCTGCGGGTCGC-3′ | ||||
arpA | 5′-AACGCTATTCGCCAGCTTGC-3′ | 400 | [14] | |
5′-TCTCCCCATACCGTACGCTA-3′ | [14,19] | |||
arpA | 5′-GATTCCATCTTGTCAAAATATGCC-3′ | 301 | [20] | |
5′-GAAAAGAAAAAGAATTCCCAAGAG-3′ | ||||
trpA | 5′-AGTTTTATGCCCAGTGCGAG-3′ | 219 | [20] | |
5′-TCTGCGCCGGTCACGCCC-3′ | ||||
Virulence factors | stx1 | 5′-TTCGCTCTGCAATAGGTA-3′ | 555 | [15] |
5′-TTCCCCAGTTCAATGTAAGAT-3′ | ||||
stx2 | 5′-GTGCCTGTTACTGGGTTTTTCTTC-3′ | 118 | [15] | |
5′-AGGGGTCGATATCTCTGTCC-3′ | ||||
eae | 5′-ATATCCGTTTTAATGGCTATCT-3′ | 425 | [15] | |
5′-AATCTTCTGCGTACTGTGTTCA-3′ | ||||
aggR | 5′-CAGAATACATCAGTACACTG-3′ | 433 | [16] | |
5′-GAAGCTTACAGCCGATATA-3′ | ||||
cnf1 | 5′-GAACTTATTAAGGATAGT-3′ | 543 | [17] | |
5′-CATTATTTATAACGCTG-3′ | ||||
cnf2 | 5′-AATCTAATTAAAGAGAGAAC-3′ 5′-CATGCTTTGTATATCTA-3′ | 543 | [17] |
Phylogroup | Antimicrobial Resistance Profile% (N) | Virulence Profile% (N) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Total | ESBL | MDR | CFO | CIP | GEN | SUT | AMI | ERT | ENO | TET | EPEC (eae) | |
A | 29 | 13.8 (4) | 24.1 (7) | 0.0 | 17.24 (5) | 10.3 (3) | 31.0 (9) | 3.5 (1) | 0.0 (0) | 10.3 (3) | 20.7 (6) | 24.1 (7) |
B1 | 4 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
B2 | 26 | 0.0 | 7.7 (2) | 3.9 (1) | 0.0 | 7.7 (2) | 26.9 (7) | 7.7 (2) | 7.7 (2) | 0.0 | 34.6 (9) | 19.2 (5) |
C | 4 | 0.0 | 50.0 (2) | 0.0 | 50.0 (2) | 0.0 | 50.0 (2) | 0.0 | 0.0 | 50.0 (2) | 50.0 (2) | 25.0 (1) |
D | 13 | 0.0 | 7.7 (1) | 0.0 | 0.0 | 7.7 (1) | 46.2 (6) | 7.7 (1) | 7.7 (1) | 7.7 (1) | 61.5 (8) | 7.7 (1) |
E | 2 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 (2) |
F | 19 | 5.3 (1) | 5.3 (1) | 0.0 | 5.3 (1) | 5.3 (1) | 5.3 (1) | 0.0 | 0.0 | 5.3 (1) | 10.5 (2) | 21.0 (4) |
Total (97) | 5.2 (5) | 13.4 (13) | 1.0 (1) | 8.2 (8) | 7.2 (7) | 25.8 (25) | 4.1 (4) | 3.1 (3) | 7.2 (7) | 27.8 (27) | 20.6 (20) |
Antimicrobial Resistance Profile | E. coli Isolates | |
---|---|---|
AMI | 1.0% | (1/97) |
CIP | 1.0% | (1/97) |
ENO | 1.0% | (1/97) |
SUT | 5.2% | (5/97) |
TET | 9.3% | (9/97) |
CIP-SUT | 1.0% | (1/97) |
SUT-TET | 10.3% | (10/97) |
AMI-ERT-TET | 1.0% | (1/97) |
CIP-ENO-GEN-SUT | 2.1% | (2/97) |
AMI-CFO-ERT-SUT-TET | 1.0% | (1/97) |
AMI-ERT-GEN-SUT-TET | 1.0% | (1/97) |
CIP-ENO-GEN-SUT-TET | 4.1% | (4/97) |
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Feitosa, C.B.; dos Santos, G.S.; Gaeta, N.C.; Schiavi, G.d.S.; Vasconcelos, C.G.C.; Filho, J.M.; Heinemann, M.B.; Cortez, A. Enteropathogenic and Multidrug-Resistant blaCTX-M-Carrying E. coli Isolates from Dogs and Cats. Animals 2024, 14, 2463. https://doi.org/10.3390/ani14172463
Feitosa CB, dos Santos GS, Gaeta NC, Schiavi GdS, Vasconcelos CGC, Filho JM, Heinemann MB, Cortez A. Enteropathogenic and Multidrug-Resistant blaCTX-M-Carrying E. coli Isolates from Dogs and Cats. Animals. 2024; 14(17):2463. https://doi.org/10.3390/ani14172463
Chicago/Turabian StyleFeitosa, Catherine Biondo, Gabriel Siqueira dos Santos, Natalia Carrillo Gaeta, Gustavo da Silva Schiavi, Carla Gasparotto Chande Vasconcelos, Jonas Moraes Filho, Marcos Bryan Heinemann, and Adriana Cortez. 2024. "Enteropathogenic and Multidrug-Resistant blaCTX-M-Carrying E. coli Isolates from Dogs and Cats" Animals 14, no. 17: 2463. https://doi.org/10.3390/ani14172463