Animals

15 pages, 2794 KiB

Open AccessArticle

A Study on the 3D Reconstruction Strategy of a Sheep Body Based on a Kinect v2 Depth Camera Array

by Jinxin Liang, Zhiyu Yuan, Xinhui Luo, Geng Chen and Chunxin Wang

Animals 2024, 14(17), 2457; https://doi.org/10.3390/ani14172457 (registering DOI) - 23 Aug 2024

Non-contact measurement based on the 3D reconstruction of sheep bodies can alleviate the stress response in sheep during manual measurement of body dimensions. However, data collection is easily affected by environmental factors and noise, which is not conducive to practical production needs. To [...] Read more.

Non-contact measurement based on the 3D reconstruction of sheep bodies can alleviate the stress response in sheep during manual measurement of body dimensions. However, data collection is easily affected by environmental factors and noise, which is not conducive to practical production needs. To address this issue, this study proposes a non-contact data acquisition system and a 3D point cloud reconstruction method for sheep bodies. The collected sheep body data can provide reference data for sheep breeding and fattening. The acquisition system consists of a Kinect v2 depth camera group, a sheep passage, and a restraining pen, synchronously collecting data from three perspectives. The 3D point cloud reconstruction method for sheep bodies is implemented based on C++ language and the Point Cloud Library (PCL). It processes noise through pass-through filtering, statistical filtering, and random sample consensus (RANSAC). A conditional voxel filtering box is proposed to downsample and simplify the point cloud data. Combined with the RANSAC and Iterative Closest Point (ICP) algorithms, coarse and fine registration are performed to improve registration accuracy and robustness, achieving 3D reconstruction of sheep bodies. In the base, 135 sets of point cloud data were collected from 20 sheep. After 3D reconstruction, the reconstruction error of body length compared to the actual values was 0.79%, indicating that this method can provide reliable reference data for 3D point cloud reconstruction research of sheep bodies. Full article

(This article belongs to the Section Small Ruminants)

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11 pages, 598 KiB

Open AccessArticle

Detection Dogs Working in Hot Climates: The Influence on Thermoregulation and Fecal Consistency

by Leopold Slotta-Bachmayr, Antony Oyugi, Noreen Mutoro, Mary Burak and Mary Wykstra

Animals 2024, 14(17), 2456; https://doi.org/10.3390/ani14172456 (registering DOI) - 23 Aug 2024

Abstract

Body temperature is an important physiological parameter that influences the performance of working dogs. The main cooling mechanism in dogs is panting to support evaporative cooling, which reduces the dog’s ability to detect scents. In this study, we investigated the general body condition [...] Read more.

Body temperature is an important physiological parameter that influences the performance of working dogs. The main cooling mechanism in dogs is panting to support evaporative cooling, which reduces the dog’s ability to detect scents. In this study, we investigated the general body condition of four detection dogs searching for cheetah scats in a hot environment in northern Kenya. We evaluated the effect on the dog’s body temperature post-work in the short term (within hours) and long term (12–24 h). The fecal consistency and mean body temperature of the investigated dogs differed significantly between individuals but not between locations (moderate Nairobi and hot Samburu). On the morning after fieldwork, the dogs showed a significantly increased body temperature (37.9 ± 0.8 °C) compared to resting days (37.5 ± 2.2 °C). In the short term, on the first day of fieldwork, the dog’s body temperature (n = 2) decreased after 10 min of rest. On the second consecutive day of fieldwork, the 10-min recovery period was too short, and the body temperature did not decrease significantly. Our data showed that the use of detection dogs in hot conditions is possible and useful but requires increased attention to prevent heat-related illness. Full article

(This article belongs to the Section Animal Welfare)

17 pages, 1711 KiB

Open AccessArticle

Determining the Optimal Harvesting Moment of Green Forage from Guizotia abyssinica Cultivated as a Catch Crop on Silage and Its Quality Form, Fresh or Wilted Green Material, in the Two Following Years

by Anna Szuba-Trznadel, Tomasz Hikawczuk, Anna Jama-Rodzeńska, Joanna Kamińska, Zlatko Svecnjak, Zygmunt Król and Bogusław Fuchs

Animals 2024, 14(17), 2455; https://doi.org/10.3390/ani14172455 (registering DOI) - 23 Aug 2024

Abstract

Guizotia abyssinica is currently being used for soil improvement; however, owing to its rapid growth and high productivity, it may have value as feed for ruminants, although this has not been well studied. Thus, this research aimed to evaluate the silage quality of [...] Read more.

Guizotia abyssinica is currently being used for soil improvement; however, owing to its rapid growth and high productivity, it may have value as feed for ruminants, although this has not been well studied. Thus, this research aimed to evaluate the silage quality of Guizotia abyssinica grown during the short season (July–October) as a catch crop in northern Europe when harvested 58, 68, and 90 days after sowing (DAS) over two production years. Ensiled material was analyzed to compare silage quality for the three different DAS. Two factors were analyzed factorially in the experiment: the silage preparation year (2018 or 2019) and the form of the ensiled material (fresh or wilted). We used 36 replications, 18 for each variant of the experimental factor. Harvesting at 58 DAS resulted in unsatisfactory forage fermentability, even after wilting. At 68 DAS, silage quality was satisfactory, but the dry matter content before ensiling was below 20% for both fresh and wilted forage, indicating limitations for silage use without additional wilting for that DAS harvest time. Dry matter content and water-soluble carbohydrates consistently increased as harvest was delayed. Thus, the highest silage quality was obtained from forage harvested 90 DAS regardless of differences in dry matter content. Therefore, it is possible to prepare silage at lower temperatures when the wilting process is limited by environmental conditions. Full article

(This article belongs to the Collection Sustainable Animal Nutrition and Feeding)

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13 pages, 969 KiB

Open AccessFeature PaperArticle

Impact of Interactions with a Puppy and Handler Versus a Handler Alone on Stress and Vitality in a University Setting: A Crossover Study

by Tiffani J. Howell, Dac L. Mai, Pam Draganovic, John-Tyler Binfet and Pauleen C. Bennett

Animals 2024, 14(17), 2454; https://doi.org/10.3390/ani14172454 (registering DOI) - 23 Aug 2024

Abstract

Brief interactions with a well-behaved dog can enhance wellbeing, but most campus-based dog visitation programs employ adult, trained dogs. There is little research about the impact of puppies on wellbeing or stress in this context. The aim of this study was to examine [...] Read more.

Brief interactions with a well-behaved dog can enhance wellbeing, but most campus-based dog visitation programs employ adult, trained dogs. There is little research about the impact of puppies on wellbeing or stress in this context. The aim of this study was to examine changes in perceived stress and vitality after interacting with a puppy. Staff and students (N = 32) at an Australian university attended the campus on two occasions, one week apart, as part of a crossover design. Participants were pseudo-randomly allocated to a group whereby they spent 20 min interacting with a handler alone at Visit 1 and interacted with a puppy and handler at Visit 2, or another group which reversed the interaction order. Perceived stress and subjective vitality were measured before and after each interaction. The increase in vitality was greater in the group experiencing the puppy and handler interaction first (significant main effect, F(1,49) = 646.89, p = 0.024, η²_p = 1.00), regardless of the visit, possibly due to a social lubricating effect by the puppy, which carried over to the ‘handler alone’ second visit. Reductions in perceived stress were greater after the interaction with the puppy, for both groups (significant interaction effect, F(1,49) = 5.13, p = 0.029, η²_p = 0.11), indicating that the puppy’s presence can reduce stress more than the handler alone. This extends the evidence for university-based dog-facilitated wellbeing programs, by showing that interactions with puppies can also be effective. This is important as it may mean that puppies already on campus as part of a socialization/training program can be incorporated into wellbeing programs for staff and students. Full article

(This article belongs to the Section Animal Welfare)

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24 pages, 9717 KiB

Open AccessArticle

Automated Measurement of Cattle Dimensions Using Improved Keypoint Detection Combined with Unilateral Depth Imaging

by Cheng Peng, Shanshan Cao, Shujing Li, Tao Bai, Zengyuan Zhao and Wei Sun

Animals 2024, 14(17), 2453; https://doi.org/10.3390/ani14172453 (registering DOI) - 23 Aug 2024

Abstract

Traditional measurement methods often rely on manual operations, which are not only inefficient but also cause stress to cattle, affecting animal welfare. Currently, non-contact cattle dimension measurement usually involves the use of multi-view images combined with point cloud or 3D reconstruction technologies, which [...] Read more.

Traditional measurement methods often rely on manual operations, which are not only inefficient but also cause stress to cattle, affecting animal welfare. Currently, non-contact cattle dimension measurement usually involves the use of multi-view images combined with point cloud or 3D reconstruction technologies, which are costly and less flexible in actual farming environments. To address this, this study proposes an automated cattle dimension measurement method based on an improved keypoint detection model combined with unilateral depth imaging. Firstly, YOLOv8-Pose is selected as the keypoint detection model and SimSPPF replaces the original SPPF to optimize spatial pyramid pooling, reducing computational complexity. The CARAFE architecture, which enhances upsampling content-aware capabilities, is introduced at the neck. The improved YOLOv8-pose achieves a mAP of 94.4%, a 2% increase over the baseline model. Then, cattle keypoints are captured on RGB images and mapped to depth images, where keypoints are optimized using conditional filtering on the depth image. Finally, cattle dimension parameters are calculated using the cattle keypoints combined with Euclidean distance, the Moving Least Squares (MLS) method, Radial Basis Functions (RBFs), and Cubic B-Spline Interpolation (CB-SI). The average relative errors for the body height, lumbar height, body length, and chest girth of the 23 measured beef cattle were 1.28%, 3.02%, 6.47%, and 4.43%, respectively. The results show that the method proposed in this study has high accuracy and can provide a new approach to non-contact beef cattle dimension measurement. Full article

(This article belongs to the Topic Precision Feeding and Management of Farm Animals, 2nd Edition)

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21 pages, 2288 KiB

Open AccessArticle

Analgesic Effect of Dexmedetomidine-Nalbuphine Combination vs. Dexmedetomidine Alone in Donkeys Undergoing Field Castration under Total Intravenous Anesthesia

by Ibrahim E. Helal, Hatim A. Al-Abbadi, Mohamed A. Hashem, Heba M. A. Abdelrazek, Mohammed H. Shekidef and Mahmoud F. Ahmed

Animals 2024, 14(17), 2452; https://doi.org/10.3390/ani14172452 (registering DOI) - 23 Aug 2024

Abstract

This study evaluated the antinociceptive effect of dexmedetomidine-nalbuphine vs. dexmedetomidine alone in jacks undergoing field castration under total intravenous anesthesia. Jacks were premedicated with intravenous (IV) dexmedetomidine (5 µg/kg), either alone (Group D, n = 6) or in combination with 0.3 mg/kg nalbuphine [...] Read more.

This study evaluated the antinociceptive effect of dexmedetomidine-nalbuphine vs. dexmedetomidine alone in jacks undergoing field castration under total intravenous anesthesia. Jacks were premedicated with intravenous (IV) dexmedetomidine (5 µg/kg), either alone (Group D, n = 6) or in combination with 0.3 mg/kg nalbuphine (Group DN, n = 6). IV ketamine (1.5 mg/kg) and propofol (0.5 mg/kg) were used to induce general anesthesia, which was maintained by a continuous propofol (0.2 mg/kg/min) IV infusion. The quality of anesthesia, analgesia, and recovery were evaluated. A simple descriptive scale (SDS) was used to measure pain from the recovery time to 6 h later. The DN group exhibited improvements in analgesic and recovery quality and SDS of pain at 1-, 2-, and 3-h post-recovery. There was an apparent improvement in cardiac status, as evidenced by the enhanced heart rate and electrocardiogram findings compared to group D during surgery and recovery time. The DN group had a lower level of inflammatory cytokines, both during the surgery and shortly after recovery. Therefore, the dexmedetomidine-nalbuphine combination prior to IV anesthesia of ketamine and propofol in jacks undergoing field castration resulted in a stable surgical plane of anesthesia, improved antinociception, less pain postoperatively, and better cardiac stability. Full article

(This article belongs to the Special Issue Recent Advances in Animals Anesthesia, Analgesia and Pain Management during Gonadectomy)

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10 pages, 4882 KiB

Open AccessCase Report

Left-to-Left Acquired Cardiac Shunt: Aorto-Left Atrial Fistula Due to Aortic Infective Endocarditis in a Dog

by Teodora Popa, Cosmin Petru Peștean, Irina Constantin, Alexandra Cofaru, Raluca Murariu, Flaviu-Alexandru Tăbăran and Iuliu Călin Scurtu

Animals 2024, 14(17), 2451; https://doi.org/10.3390/ani14172451 (registering DOI) - 23 Aug 2024

Abstract

Infective endocarditis is a severe but rarely diagnosed disease, characterized by the presence of bacterial infection at the level of the cardiac valves. Although the incidence of the disease is very low, the consequences are severe and the prognosis is very poor, outlining [...] Read more.

Infective endocarditis is a severe but rarely diagnosed disease, characterized by the presence of bacterial infection at the level of the cardiac valves. Although the incidence of the disease is very low, the consequences are severe and the prognosis is very poor, outlining a high mortality rate among cases. The present report highlights the case of a 7-year-old dog presented with abrupt changes in the respiratory pattern, obtunded and in lateral recumbency. The physical examination of the patient revealed fever and a IV/VI systolic heart murmur, with the point of maximal intensity on the left hemithorax. Echocardiography identified hyperechoic and cavitary changes beneath the aortic valves and a retrograde turbulent jet originating in the left ventricle outflow tract communicating with the left atrium through a rupture in the aortomitral intervalvular wall. Because of very unstable hemodynamic changes, the dog suddenly died despite the initiation of intensive care supportive treatment, and the postmortem evaluation of the heart confirms the suspicion of infective aortic endocarditis with the development of a paravalvular abscess and an aorto-left atrial fistula. Full article

(This article belongs to the Collection Clinical Pathology in Animals)

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16 pages, 5060 KiB

Open AccessArticle

Ningxiang Pig-Derived Microbiota Affects the Growth Performance, Gut Microbiota, and Serum Metabolome of Nursery Pigs

by Hongkun Li, Li Han, Feng Zhou, Zichen Wu, Longlin Zhang, Renjie Xie, Feng Jiang, Qiyu Tian and Xingguo Huang

Animals 2024, 14(17), 2450; https://doi.org/10.3390/ani14172450 (registering DOI) - 23 Aug 2024

Abstract

The gut microbiota is crucial for maintaining the host’s intestinal homeostasis and metabolism. This study investigated the effects of fecal microbiota transplantation (FMT) from Ningxiang pigs on the growth performance, fecal microbiota, and serum metabolites of the same-old DLY pigs. The results indicated [...] Read more.

The gut microbiota is crucial for maintaining the host’s intestinal homeostasis and metabolism. This study investigated the effects of fecal microbiota transplantation (FMT) from Ningxiang pigs on the growth performance, fecal microbiota, and serum metabolites of the same-old DLY pigs. The results indicated that the average daily gain of FMT pigs was significantly greater than that of the control (CON) group. Compared to the CON group, the FMT group significantly improved the apparent digestibility of crude fiber, crude ash, gross energy, and calcium of the pigs. The analysis of serum antioxidant status revealed that the activities of total superoxide dismutase and catalase in the serum of pigs in the FMT group were significantly elevated, whereas the level of malondialdehyde was significantly reduced. Furthermore, 16S rRNA sequencing analysis revealed that the Ningxiang pig-derived microbiota altered the fecal microbiota structure and modulated the diversity of the gut microbiota in the DLY pigs. Untargeted LC–MS metabolomics demonstrated that pigs in the FMT group exhibited distinct metabolomic profiles compared to those in the CON group. Significant changes were observed in key metabolites involved in amino acid, lipid, and carbohydrate metabolism. Additionally, a correlation analysis between serum differential metabolites and the gut microbiota revealed that the relative abundance of Lachnospiraceae_NK4A136_group and Corynebacterium was highly correlated with lipid compounds. In conclusion, Ningxiang pig-derived microbiota can alleviate oxidative stress and enhance growth performance in DLY pigs by modulating their gut microbiota and metabolic features. Full article

(This article belongs to the Section Pigs)

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16 pages, 3057 KiB

Open AccessArticle

Genetic Structure and Genome-Wide Association Analysis of Growth and Reproductive Traits in Fengjing Pigs

by Lei Xing, Xuelin Lu, Wengang Zhang, Qishan Wang and Weijian Zhang

Animals 2024, 14(17), 2449; https://doi.org/10.3390/ani14172449 (registering DOI) - 23 Aug 2024

Abstract

The Fengjing pig is one of the local pig breed resources in China and has many excellent germplasm characteristics. However, research on its genome is lacking. To explore the degree of genetic diversity of the Fengjing pig and to deeply explore its excellent [...] Read more.

The Fengjing pig is one of the local pig breed resources in China and has many excellent germplasm characteristics. However, research on its genome is lacking. To explore the degree of genetic diversity of the Fengjing pig and to deeply explore its excellent traits, this study took Fengjing pigs as the research object and used the Beadchip Array Infinium iSelect-96|XT KPS_PorcineBreedingChipV2 for genotyping. We analyzed the genetic diversity, relatedness, inbreeding coefficient, and population structure within the Fengjing pig population. Our findings revealed that the proportion of polymorphic markers (P_N) was 0.469, and the effective population size was 6.8. The observed and expected heterozygosity were 0.301 and 0.287, respectively. The G-matrix results indicated moderate relatedness within the population, with certain individuals exhibiting closer genetic relationships. The NJ evolutionary tree classified Fengjing boars into five family lines. The average inbreeding coefficient based on ROH was 0.318, indicating a high level of inbreeding. GWAS identified twenty SNPs significantly associated with growth traits (WW, 2W, and 4W) and reproductive traits (TNB and AWB). Notably, WNT8B, RAD21, and HAO1 emerged as candidate genes influencing 2W, 4W, and TNB, respectively. Genes such as WNT8B were verified by querying the PigBiobank database. In conclusion, this study provides a foundational reference for the conservation and utilization of Fengjing pig germplasm resources and offers insights for future molecular breeding efforts in Fengjing pigs. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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14 pages, 12789 KiB

Open AccessArticle

The Regulatory Role of Myomaker in the Muscle Growth of the Chinese Perch (Siniperca chuatsi)

by Wei Zeng, Yangyang Meng, Lingtao Nie, Congyi Cheng, Zexia Gao, Lusha Liu, Xin Zhu and Wuying Chu

Animals 2024, 14(17), 2448; https://doi.org/10.3390/ani14172448 (registering DOI) - 23 Aug 2024

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Abstract

The fusion of myoblasts is a crucial stage in the growth and development of skeletal muscle. Myomaker is an important myoblast fusion factor that plays a crucial role in regulating myoblast fusion. However, the function of Myomaker in economic fish during posthatching has [...] Read more.

The fusion of myoblasts is a crucial stage in the growth and development of skeletal muscle. Myomaker is an important myoblast fusion factor that plays a crucial role in regulating myoblast fusion. However, the function of Myomaker in economic fish during posthatching has been poorly studied. In this study, we found that the expression of Myomaker in the fast muscle of Chinese perch (Siniperca chuatsi) was higher than that in other tissues. To determine the function of Myomaker in fast muscle, Myomaker-siRNA was used to knockdown Myomaker in Chinese perch and the effect on muscle growth was determined. The results showed that the growth of Chinese perch was significantly decreased in the Myomaker-siRNA group. Furthermore, both the diameter of muscle fibers and the number of nuclei in single muscle fibers were significantly reduced in the Myomaker-siRNA group, whereas there was no significant difference in the number of BrdU-positive cells (proliferating cells) between the control and the Myomaker-siRNA groups. Together, these findings indicate that Myomaker may regulate growth of fast muscle in Chinese perch juveniles by promoting myoblast fusion rather than proliferation. Full article

(This article belongs to the Section Aquatic Animals)

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