Integration of Gut Microbiota with Transcriptomic and Metabolomic Profiling Reveals Growth Differences in Male Giant River Prawns (Macrobrachium rosenbergii)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Bacterial Genomic DNA Extraction and Sequencing
2.3. Metabolite Extraction
2.4. LC-MS/MS Analysis
2.5. Correlation Analysis
3. Results
3.1. Gut Microbial Community Composition
3.2. Identification and Analysis of Plasma Metabolites in Male GFP with Different Growth Rates
3.3. Correlation between Gut Bacteria, Transcripts, Phenotypes, and Metabolism in Male GFPs
3.4. Integrative Multiomic Analysis of Transcripts, Microbiome, Phenotypes, and Metabolites in Male GFPs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Metabolite | Pathway | Average Abundance in Each Group | ||
---|---|---|---|---|
ML | MM | MS | ||
Picolinic acid | Tryptophan metabolism | 2,512,533,397 | 2,783,737,035 | 3,886,922,059 |
L-Tyrosine | Methane metabolism | 1,380,214,938 | 818,298,529.6 | 494,550,907.6 |
Succinic acid | Biosynthesis of terpenoids and steroids | 207,214,268.1 | 138,050,153 | 31,549,879.95 |
Aminocaproic acid | Caprolactam degradation | 162,473,612.3 | 143,181,951.9 | 220,255,718.7 |
Phosphorylcholine | Glycerophospholipid metabolism | 149,938,816 | 95,324,908.67 | 91,402,453.03 |
L-Methionine | Mineral absorption | 126,393,703 | 116,471,294.1 | 183,369,889 |
L-Histidine | Aminoacyl-tRNA biosynthesis | 65,508,706.11 | 63,207,424.53 | 68,971,672.67 |
L-Asparagine | Protein digestion and absorption | 54,059,698.19 | 47,438,258.65 | 79,577,739.17 |
O-Phosphoethanolamine | Sphingolipid pathway | 50,654,144.98 | 49,672,681.24 | 70,910,504.34 |
Acetylcholine | Taste transduction | 39,034,131.15 | 19,257,807.04 | 21,312,743.56 |
Sorbitol | ABC transporters | 24,872,146.19 | 17,401,326.46 | 19,175,258.42 |
L-Norvaline | \ | 20,880,672.75 | 40,609,717.36 | 110,409,043 |
Citrulline | Arginine biosynthesis | 20,244,481.76 | 95,438,311.48 | 124,961,442.1 |
Ribose 1,5-bisphosphate | Phosphonate and phosphinate metabolism | 11,435,523.25 | 9,671,995.33 | 8,929,543.9 |
2-Ketobutyric acid | Biosynthesis of amino acids | 10,922,998.81 | 8,515,497.23 | 4,952,491.33 |
L-Ribulose | Pentose and glucuronate interconversions | 3,659,200.9 | 3,401,882.38 | 7,809,899.52 |
Trehalose | Phosphotransferase system (PTS) | 1,774,509.08 | 1614,878.04 | 1,183,823 |
Punicic acid | \ | 1,144,145.89 | 1,470,456.6 | 605,040.97 |
Maleic acid | Butanoate metabolism | 1,045,720.79 | 5,604,645.41 | 16,113,880.62 |
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Gao, Q.; Huang, H.; Liu, P.; Zhao, X.; Tang, Q.; Xia, Z.; Cai, M.; Wang, R.; Huang, G.; Yi, S. Integration of Gut Microbiota with Transcriptomic and Metabolomic Profiling Reveals Growth Differences in Male Giant River Prawns (Macrobrachium rosenbergii). Animals 2024, 14, 2539. https://doi.org/10.3390/ani14172539
Gao Q, Huang H, Liu P, Zhao X, Tang Q, Xia Z, Cai M, Wang R, Huang G, Yi S. Integration of Gut Microbiota with Transcriptomic and Metabolomic Profiling Reveals Growth Differences in Male Giant River Prawns (Macrobrachium rosenbergii). Animals. 2024; 14(17):2539. https://doi.org/10.3390/ani14172539
Chicago/Turabian StyleGao, Quanxin, Hao Huang, Peimin Liu, Xiuxin Zhao, Qiongying Tang, Zhenglong Xia, Miuying Cai, Rui Wang, Guanghua Huang, and Shaokui Yi. 2024. "Integration of Gut Microbiota with Transcriptomic and Metabolomic Profiling Reveals Growth Differences in Male Giant River Prawns (Macrobrachium rosenbergii)" Animals 14, no. 17: 2539. https://doi.org/10.3390/ani14172539