Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Boar Sperm Preparation and Capacitation
2.1.1. Sperm Density Gradient Selection (DGS)
2.1.2. Microfluidics Chip-Based Sperm Separation (MCS)
2.2. Boar Sperm Evaluation
2.2.1. Sperm Morphology
2.2.2. Total Motility
2.2.3. Sperm Concentration
2.2.4. Sperm Viability and Acrosomal Integrity
2.2.5. Sperm DNA Damage Testing
2.3. Porcine Oocyte Collection and in Vitro Maturation (IVM)
2.4. Porcine in Vitro Fertilisation and Embryo Culture
2.5. Evaluation of Pig Blastocysts
2.6. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Head Abn. Mean% ± SEM | Tail Abn. Mean% ± SEM | CD Mean% ± SEM |
---|---|---|---|
Control | 7.6 ± 0.7 | 10.5 ± 1.2 a | 11.5 ± 1.6 a |
MCS | 6.7 ± 0.5 | 4.9 ± 0.6 b | 3.6 ± 0.6 b |
DGS | 7.4 ± 0.5 | 8.6 ± 1.0 c | 8.8 ± 1.2 c |
p value * | 0.402 | <0.001 | <0.001 |
H * | 1.82 | 16.744 | 19.383 |
Group | DFI Mean% ± SEM | HDS Mean% ± SEM |
---|---|---|
Control | 2.4 ± 0.3 a | 2.3 ± 0.4 a |
MCS | 0.7 ± 0.1 b | 0.7 ± 0.1 b |
DGS | 1.1 ± 0.2 b | 1.7 ± 0.3 a |
p value * | <0.001 | <0.001 |
H * | 27.76 | 27.53 |
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Serrano-Albal, M.; Aquilina, M.C.; Kiazim, L.G.; Zak, L.J.; Griffin, D.K.; Ellis, P.J. Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes. Animals 2024, 14, 2544. https://doi.org/10.3390/ani14172544
Serrano-Albal M, Aquilina MC, Kiazim LG, Zak LJ, Griffin DK, Ellis PJ. Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes. Animals. 2024; 14(17):2544. https://doi.org/10.3390/ani14172544
Chicago/Turabian StyleSerrano-Albal, Maria, Marie Claire Aquilina, Lucas G. Kiazim, Louisa J. Zak, Darren K. Griffin, and Peter J. Ellis. 2024. "Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes" Animals 14, no. 17: 2544. https://doi.org/10.3390/ani14172544