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Chlorogenic Acid Plays an Important Role in Improving the Growth and Antioxidant Status and Weakening the Inflammatory Response of Largemouth Bass (Micropterus salmoides)
by
, , ,
Zetian Xia
1,
Haifeng Mi
2,
Mingchun Ren
1,3,
Dongyu Huang
3,
Ahmed Mohamed Aboseif
4,
Hualiang Liang
1,3,* and 
Lu Zhang
2,* 1
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China
2
Tongwei Agricultural Development Co., Ltd., Key Laboratory of Nutrition and Healthy Culture of Aquatic, Livestock and Poultry, Ministry of Agriculture and Rural Affairs, Healthy Aquaculture Key Laboratory of Sichuan Province, Chengdu 610093, China
3
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
4
National Institute of Oceanography and Fisheries (NIOF), Academy of Scientific Research and Technology (ASRT), Cairo 11796, Egypt
*
Authors to whom correspondence should be addressed.
Animals 2024, 14(19), 2871; https://doi.org/10.3390/ani14192871 (registering DOI)
Submission received: 31 August 2024
/
Revised: 2 October 2024
/
Accepted: 3 October 2024
/
Published: 5 October 2024
(This article belongs to the Section Aquatic Animals)
Simple Summary
This study examined the effects of chlorogenic acid (CGA) on largemouth bass’s growth, immunity, and antioxidant status. Over eight weeks, fish were fed diets with varying CGA levels. In this study, CGA improved the growth performance and lowered the FCR. In addition, CGA enhanced the antioxidant capacity and regulated the expression of genes related to immune responses, thereby strengthening immunity. Overall, the addition of CGA positively impacted the health and growth of largemouth bass.
Abstract
This experiment evaluated the function of chlorogenic acid (CGA) in the growth, health status, and inflammation of largemouth bass (Micropterus salmoides). Over eight weeks, CGA supplementation was designed at five levels: 0, 60, 120, 180, and 240 mg/kg. The 180 and 240 mg/kg CGA-supplemented groups showed significant improvements in the FBW, SGR, and WGR compared to the control group (0 mg/kg) (p < 0.05). All the CGA-supplemented groups exhibited a significant reduction in the FCR (p < 0.05), with the 180 mg/kg CGA group showing the lowest FCR. Nonetheless, there were no appreciable differences in the plasma concentrations of TP, ALT, or AST among the treatments (p > 0.05). Compared to the control group, the 180 mg/kg CGA group exhibited significantly lower TC and TG levels (p < 0.05). The ALP levels showed no significant differences from the control group (p > 0.05). In terms of antioxidant parameters, CGA supplementation considerably reduced the MDA content (p < 0.05) and increased the GSH levels, while decreasing the CAT, SOD, and GPx activity levels Meanwhile, CGA supplementation resulted in reduced mRNA levels of SOD, CAT, Nrf2, Keap1, and NF-κB compared to the control group. In contrast, the mRNA levels of GPx, IL-8, TLR2, and RelA were elevated in the liver. Our findings indicated that CGA supplementation improved the growth performance and antioxidant status and weakened the inflammatory response of largemouth bass. These findings suggest that CGA could be a valuable dietary supplement for enhancing the health and growth of this species.
