Animals

10 pages, 548 KiB

Open AccessArticle

Evaluation of the Effect of a New Skin Fixation Technique to Avoid Shrinkage of Skin Samples Obtained from Canine Cadavers

by Ligita Zorgevica-Pockevica, Nataliia Kuzhel, Sigita Kerziene and Simona Vincenti

Animals 2024, 14(19), 2791; https://doi.org/10.3390/ani14192791 (registering DOI) - 26 Sep 2024

Abstract

Skin shrinkage begins immediately after surgical incision and is an artefact associated with the excision and fixation of a specimen. Skin shrinkage results in important changes in histologic tissue dimensions and can affect the correct quantification of the histologic tumour-free margin (HTFM). Bilateral [...] Read more.

Skin shrinkage begins immediately after surgical incision and is an artefact associated with the excision and fixation of a specimen. Skin shrinkage results in important changes in histologic tissue dimensions and can affect the correct quantification of the histologic tumour-free margin (HTFM). Bilateral and symmetrical circular skin samples with a diameter of 60 mm were taken from the lateral thoracic, flank and femoral regions of dog cadavers, with the samples from one side belonging to the study group and the samples from the same animal from the other side belonging to the control group. The radius and diameter of the specimen were measured immediately after the excision and 10 min later for each sample. The measurements of the study group were taken again after manual re-extension and fixation on a cork plate before formalin fixation and 48 h after formalin fixation. A total of 66 (33 study and 33 control group) samples were collected from 11 canine cadavers. The mean diameter shrinkage after formalin fixation was 18.24% for the control group and 0.64% for the study group. A statistically significant difference between the study and the control group was found (p < 0.001). This method of specimen fixation in the study group avoided skin shrinkage and deformation of the specimen in formalin, which we believe improves the diagnostic accuracy of surgical margins and, thus, reduces the number of false-positive or false-negative HTFM. Full article

(This article belongs to the Section Veterinary Clinical Studies)

12 pages, 2039 KiB

Open AccessArticle

Corvisyringophilus, a New Genus in the Family Syringophilidae (Acariformes: Prostigmata) and Its Phylogenetic Position among Primitive Genera

by Maciej Skoracki, Ólafur K. Nielsen and Bozena Sikora

Animals 2024, 14(19), 2790; https://doi.org/10.3390/ani14192790 (registering DOI) - 26 Sep 2024

Abstract

Syringophilidae is one of the most species-rich families in the superfamily Cheyletoidea, comprising approximately 420 species across 62 genera and two subfamilies. In this paper, we propose a new genus, Corvisyringophilus, and a new species, C. krummi gen. n. et sp. n., [...] Read more.

Syringophilidae is one of the most species-rich families in the superfamily Cheyletoidea, comprising approximately 420 species across 62 genera and two subfamilies. In this paper, we propose a new genus, Corvisyringophilus, and a new species, C. krummi gen. n. et sp. n., found in the wing covert quills of the Common Raven, Corvus corax Linnaeus, in Iceland. Corvisyringophilus is placed among the primitive genera of syringophilid mites, which possess the full complement of idiosomal and leg setae. Phylogenetic analysis based on morphological characters suggests that this genus forms a sister clade to Blaszakia Skoracki & Sikora, 2008, and Charadriphilus Bochkov & Mironov, 1998, which inhabit birds of the orders Musophagiformes and Charadriiformes, respectively. The study proposes that the current distribution patterns of quill mites, based on their morphological characteristics, may result from multiple host switching followed by co-speciation events, highlighting the complex evolutionary dynamics within this family. Full article

(This article belongs to the Section Ecology and Conservation)

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15 pages, 415 KiB

Open AccessArticle

Comparing the Performance of Automatic Milking Systems through Dynamic Testing Also Helps to Identify Potential Risk Factors for Mastitis

by Stefano Milanesi, Dario Donina, Viviana Chierici Guido, Francesca Zaghen, Valerio M. Sora and Alfonso Zecconi

Animals 2024, 14(19), 2789; https://doi.org/10.3390/ani14192789 - 26 Sep 2024

Abstract

Automatic milking systems (AMSs) are revolutionizing the dairy industry by boosting herd efficiency, primarily through an increased milk yield per cow and reduced labor costs. The performance of milking machines, whether traditional or automated, can be evaluated using advanced vacuum meters through dynamic [...] Read more.

Automatic milking systems (AMSs) are revolutionizing the dairy industry by boosting herd efficiency, primarily through an increased milk yield per cow and reduced labor costs. The performance of milking machines, whether traditional or automated, can be evaluated using advanced vacuum meters through dynamic testing. This process involves scrutinizing the system and milking routine to identify critical points, utilizing the VaDia™ logger (BioControl AS, Rakkestad, Norway). Vacuum recordings were downloaded and analyzed using the VaDia Suite™ software under the guidance of a milking specialist. Access to data from AMSs across various manufacturers and herds facilitated a retrospective study aimed at describing and comparing key milk emission parameters for different AMS brands while identifying potential mastitis risk factors. Using the proper statistical procedures of SPSS 29.1 (IBM Corp., Armonk, NY, USA), researchers analyzed data from 4878 individual quarter milkings from cows in 48 dairy herds. Results indicated a significant variability in milking parameters associated with quarter milk yield and AMS brand. Notably, despite AMSs standardizing teat preparation and stimulation, this study revealed a surprisingly high frequency of two major mastitis risk factors—bimodality and irregular vacuum fluctuations—occurring more frequently than in conventional milking systems. This study, one of the few comparing different AMS brands and their performance, highlights the crucial role of dynamic testing in evaluating AMS performance under real-world conditions. Full article

(This article belongs to the Special Issue Factors Affecting Milk Productivity, Milk Quality, and Dairy Cow and Calves Health—2nd Edition)

16 pages, 844 KiB

Open AccessArticle

Genomic and Pathological Characterization of Acute Hepatopancreatic Necrosis Disease (AHPND)-Associated Natural Mutant Vibrio parahaemolyticus Isolated from Penaeus vannamei Cultured in Korea

by Ye Bin Kim, Seon Young Park, Hye Jin Jeon, Bumkeun Kim, Mun-Gyeong Kwon, Su-Mi Kim, Jee Eun Han and Ji Hyung Kim

Animals 2024, 14(19), 2788; https://doi.org/10.3390/ani14192788 (registering DOI) - 26 Sep 2024

Abstract

Acute hepatopancreatic necrosis disease (AHPND) is one of the most important diseases in the global shrimp industry. The emergence of mutant AHPND-associated V. parahaemolyticus (Vp_AHPND) strains has raised concerns regarding potential misdiagnosis and unforeseen pathogenicity. In this study, we [...] Read more.

Acute hepatopancreatic necrosis disease (AHPND) is one of the most important diseases in the global shrimp industry. The emergence of mutant AHPND-associated V. parahaemolyticus (Vp_AHPND) strains has raised concerns regarding potential misdiagnosis and unforeseen pathogenicity. In this study, we report the first emergence of a type II (pirA⁻, pirB⁺) natural mutant, Vp_AHPND (strain 20-082A3), isolated from cultured Penaeus vannamei in Korea. Phenotypic and genetic analyses revealed a close relationship between the mutant strain 20-082A3 and the virulent Korean Vp_AHPND strain 19-021-D1, which caused an outbreak in 2019. Detailed sequence analysis of AHPND-associated plasmids showed that plasmid pVp_20-082A3B in strain 20-082A3 was almost identical (>99.9%) to that of strain 19-021-D1. Moreover, strains 20-082A3 and 19-021-D1 exhibited the same multilocus sequence type (ST 413) and serotype (O1:Un-typeable K-serogroup), suggesting that the mutant strain is closely related to and may have originated from the virulent strain 19-021-D1. Similar to previous reports on the natural mutant Vp_AHPND, strain 20-082A3 did not induce AHPND-related symptoms or cause mortality in the shrimp bioassay. The emergence of a mutant strain which is almost identical to the virulent Vp_AHPND highlights the need for surveillance of the pathogen prevalent in Korea. Further investigations to elucidate the potential relationship between ST 413 and recent Korean Vp_AHPND isolates are needed. Full article

(This article belongs to the Special Issue Bacterial and Viral Diseases in Aquatic Animals)

15 pages, 2221 KiB

Open AccessArticle

Comparison between a Flash Glucose Monitoring System and a Portable Blood Glucose Meter for Monitoring of Cats with Diabetic Ketosis or Ketoacidosis

by Jennifer S. Eiermann, Katarina Hazuchova, Beatriz Vidondo, Miguel Campos, Simone Schuller and Yi Cui

Animals 2024, 14(19), 2787; https://doi.org/10.3390/ani14192787 - 26 Sep 2024

Abstract

Cats with diabetic ketosis or ketoacidosis DK(A) require intensive glucose monitoring. The aim of this study was to assess the agreement between a portable blood glucose meter (PBGM) and a flash glucose monitoring system (FGMS; FreeStyle Libre 2.0 Abbott^®) measuring interstitial [...] Read more.

Cats with diabetic ketosis or ketoacidosis DK(A) require intensive glucose monitoring. The aim of this study was to assess the agreement between a portable blood glucose meter (PBGM) and a flash glucose monitoring system (FGMS; FreeStyle Libre 2.0 Abbott^®) measuring interstitial glucose in cats with DK(A). Ten client-owned cats with naturally occurring DK(A) were prospectively enrolled. Glucose concentrations were assessed with both methods every 1–3 h until resolution of DK(A), and every 4–8 h thereafter. While the median FGMS measured glucose concentration (14.3 mmol/L) was significantly lower than the median PBGM measured glucose concentration (19 mmol/L) (p < 0.001), the overall correlation between the FGMS and PBGM was high (r = 0.88; p < 0.001). In the Parkes error grid analysis, 98.3% of measurements fell in zones A and B. Bland–Altman plot analysis demonstrated that in the low glycaemic range (BG < 5.5 mmol/L), 50% of FGMS measurements deviated more than ±0.83 mmol/L, and in the high glycaemic range (BG > 5.5 mmol/L), 81% of results deviated >15% from the PBGM measurements. There was significant inter-individual variation in the difference between glucose concentrations measured by the FGMS and PBGM (p < 0.001). In spite of being more easily tolerated and easier to use, currently this method cannot be recommended for routine monitoring of cats with DK(A). Full article

(This article belongs to the Section Veterinary Clinical Studies)

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19 pages, 5009 KiB

Open AccessArticle

Dietary Fermented Blueberry Pomace Supplementation Improves Small Intestinal Barrier Function and Modulates Cecal Microbiota in Aged Laying Hens

by Binghua Qin, Zhihua Li, Qian Zhu, Ting Chen, Wei Lan, Yadong Cui, Md. Abul Kalam Azad and Xiangfeng Kong

Animals 2024, 14(19), 2786; https://doi.org/10.3390/ani14192786 (registering DOI) - 26 Sep 2024

Abstract

This study aimed to investigate the effects of fermented blueberry pomace (FBP) on the intestinal barrier function and cecal microbiome of aged laying hens. A total of 320 Yukou Jingfen No. 8 laying hens (345-day-old) were randomly divided into a control group, 0.25% [...] Read more.

This study aimed to investigate the effects of fermented blueberry pomace (FBP) on the intestinal barrier function and cecal microbiome of aged laying hens. A total of 320 Yukou Jingfen No. 8 laying hens (345-day-old) were randomly divided into a control group, 0.25% FBP group, 0.5% FBP group, or 1.0% FBP group. The results showed that the villus height (VH) in the jejunum of the 0.25-0.5% FBP groups and villus surface area in the jejunum of the 0.25% FBP group were higher (p < 0.05), while 0.25% FBP supplementation displayed a higher (p = 0.070) VH in the ileum compared to the control group. Mucin-2 expression was upregulated (p < 0.05) in the jejunum of the 0.5% FBP group and the ileum of the 0.25-0.5% FBP groups. Compared to the control group, interleukin (IL)-4 and IL-13 expressions were upregulated (p < 0.05) in the 1.0% FBP group. Microbiota analysis revealed that Prevotella abundance in the cecum of the 0.5-1.0% FBP groups was higher (p < 0.05) than in the 0.25% FBP group. In addition, microbial function prediction analysis showed that cecal microbiota in the 0.25% FBP group were mainly enriched by alanine/aspartate/glutamate metabolism and methane metabolism. Moreover, Spearman’s correlation analysis revealed the potential correlations between the abundance of the cecal microbiota and intestinal-barrier-function-related gene expressions, as well as the short-chain fatty acid content, of laying hens. In summary, dietary FBP supplementation enhanced intestinal barrier function by improving intestinal morphology, upregulating gene expressions related to barrier function, and altering the cecal microbiota of aged laying hens. Full article

(This article belongs to the Collection Use of Agricultural By-Products in Animal Feeding)

12 pages, 334 KiB

Open AccessArticle

The Characteristics of Milk Fatty Acid Profile Predicted by Fourier-Transform Mid-Infrared Spectroscopy (FT-MIRS) in Chinese Holstein Cows

by Chunfang Li, Haitong Wang, Yikai Fan, Zengpo Zhou, Yuanbao Li, Shengchao Liang, Yabin Ma and Shujun Zhang

Animals 2024, 14(19), 2785; https://doi.org/10.3390/ani14192785 (registering DOI) - 26 Sep 2024

Abstract

Fatty acid is an important factor affecting the nutritional quality of milk. In this study, we collected and assessed 78,086 milk samples from 12,065 Chinese Holstein cows from 11 farms in Northern China from November 2019 to September 2022. The contents of eight [...] Read more.

Fatty acid is an important factor affecting the nutritional quality of milk. In this study, we collected and assessed 78,086 milk samples from 12,065 Chinese Holstein cows from 11 farms in Northern China from November 2019 to September 2022. The contents of eight fatty acid groups were predicted using FT-MIRS-based models. The contents of TFAs, SFAs, UFAs, MUFAs, PUFAs, and LCFAs in milk reached the highest at 96–125 DIM, and SCFA and MCFA contents reached the highest at 276–305 DIM. With the increase in somatic cell score, the contents of various fatty acid groups in milk gradually decreased, and the nutritional value of milk and flavor of dairy products gradually deteriorated. The contents of high-quality fatty acids in milk, particularly UFAs and MUFAs, were significantly higher in the non-pregnant state than in the pregnant state. However, SCFA and MCFA contents exhibited the opposite pattern. Our findings provided valuable information on the content and distribution range of fatty acid groups in milk from Chinese Holstein cows. Further analysis is warranted to explore the breeding of Chinese Holstein cows providing milk with abundant beneficial fatty acids. Full article

(This article belongs to the Section Cattle)

17 pages, 3166 KiB

Open AccessArticle

Genetic Diversity and Selection Signal Analysis of Hu Sheep Based on SNP50K BeadChip

by Keyan Ma, Juanjuan Song, Dengpan Li, Taotao Li and Youji Ma

Animals 2024, 14(19), 2784; https://doi.org/10.3390/ani14192784 (registering DOI) - 26 Sep 2024

Abstract

This research is designed to examine the genetic diversity and kinship among Hu sheep, as well as to discover genes associated with crucial economic traits. A selection of 50 unrelated adult male Hu sheep underwent genotyping with the SNP50K BeadChip. Seven indicators of [...] Read more.

This research is designed to examine the genetic diversity and kinship among Hu sheep, as well as to discover genes associated with crucial economic traits. A selection of 50 unrelated adult male Hu sheep underwent genotyping with the SNP50K BeadChip. Seven indicators of genetic diversity were assessed based on high-quality SNP data: effective population size (Ne), polymorphic information content (PIC), polymorphic marker ratio (P_N), expected heterozygosity (He), observed heterozygosity (Ho), effective number of alleles, and minor allele frequency (MAF). Plink software was employed to compute the IBS genetic distance matrix and detect runs of homozygosity (ROHs), while the G matrix and principal component analysis were performed using GCTA software. Selective sweep analysis was carried out using ROH, Pi, and Tajima’s D methodologies. This study identified a total of 64,734 SNPs, of which 56,522 SNPs remained for downstream analysis after quality control. The population displayed relatively high genetic diversity. The 50 Hu sheep were ultimately grouped into 12 distinct families, with families 6, 8, and 10 having the highest numbers of individuals, each consisting of 6 sheep. Furthermore, a total of 294 ROHs were detected, with the majority having lengths between 1 and 5 Mb, and the inbreeding coefficient F_ROH was 0.01. In addition, 41, 440, and 994 candidate genes were identified by ROH, Pi, and Tajima’s D methods, respectively, with 3 genes overlapping (BMPR1B, KCNIP4, and FAM13A). These results offer valuable insights for future Hu sheep breeding, genetic assessment, and population management. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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17 pages, 1948 KiB

Open AccessArticle

Influence of Maternal Dietary Protein during Late Gestation on Performance of Black Bengal Does and Their Kids

by Md Sayaduzzaman Arafath, Mahadi Hasan, Jakia Sultana, Md Hasanur Alam, Asma Khatun and Mohammad Moniruzzaman

Animals 2024, 14(19), 2783; https://doi.org/10.3390/ani14192783 (registering DOI) - 26 Sep 2024

Abstract

The present study aimed to elucidate the effect of different levels of dietary protein during late pregnancy on the performance of Black Bengal does and their kids. Twelve does were divided into three groups, with four in each, and three diets, i.e., high [...] Read more.

The present study aimed to elucidate the effect of different levels of dietary protein during late pregnancy on the performance of Black Bengal does and their kids. Twelve does were divided into three groups, with four in each, and three diets, i.e., high protein (18% CP), medium protein (14% CP), and low protein (10% CP) were supplied for 50 days, commencing from 100 days post-coitum to parturition. During the first 100 days of pregnancy, uniform rations with similar ingredients were provided to fulfill the nutrient requirements depending on the live weight of does. All three diets were isocaloric (10.0 MJ/kg DM). Data were subjected to one-way ANOVA, and the significance of the difference among means was determined by Duncan’s Multiple Range Test (DMRT). The main effects of diet and sex, as well as their interaction, were analyzed by two-way ANOVA by using the GLM procedure. The relative expression values of qPCR were calculated by using the 2^−ΔΔCt analysis method. Live weight gain was significantly (p < 0.05) higher in high-protein-fed dams than other groups during the experimental period. The milk yield of does was significantly (p < 0.05) higher in high-protein-fed goats than in the low-protein group. The lactation length of does was significantly (p < 0.05) higher in the high- and medium-protein-fed does than in the low-protein-fed does. The duration of post-partum anestrus of does was significantly (p < 0.05) higher in the low-protein-fed dams than in the high-protein group. The birth weight of kids tended to be higher in the high-protein group but did not differ significantly among the treatment groups. In male kids, weaning weight, final weight, live weight gain, and average daily gain were significantly (p < 0.05) higher than in female kids. Weaning weight was higher (p < 0.05) in kids of the high-protein-fed does than the low-protein group. Final weight and live weight gain were significantly (p < 0.05) higher in kids of the high-protein-fed does than in the low-protein-fed group. On the other hand, average daily gain was significantly (p < 0.05) higher in kids of the high- and medium-protein-fed does than the low-protein group. The average body length and wither height of kids at the 32nd week was significantly (p < 0.05) higher in kids of high-protein-fed does than those of the low-protein-fed group. The average heart girth of kids at the 32nd week was significantly (p < 0.05) higher in kids of high-protein-fed does than the medium- and low-protein groups. The survival rate of kids was higher in the medium- and high-protein-fed does than in low-protein group. Hot carcass weight and ether extract content of meat were significantly (p < 0.05) higher in the high-protein group than in the other groups. The dressing percentage was significantly (p < 0.05) higher in the kids of high-protein-fed does than low-protein-fed goats. The expression of the H-FABP gene was significantly (p < 0.05) higher in kids of high-protein-fed does than those of the medium- and low-protein groups. In conclusion, maternal dietary protein levels positively influences the production performance of Black Bengal does and their kids. Full article

(This article belongs to the Special Issue Nutrient Utilization, Requirements and Nutrigenomics in Sheep and Goats)

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16 pages, 2203 KiB

Open AccessArticle

A Snapshot of the Global Trade of South African Native Vertebrate Species Not Listed on CITES

by Ndivhuwo Shivambu, Tinyiko Cavin Shivambu, Takalani Nelufule, Moleseng Claude Moshobane, Nimmi Seoraj-Pillai and Tshifhiwa Constance Nangammbi

Animals 2024, 14(19), 2782; https://doi.org/10.3390/ani14192782 (registering DOI) - 26 Sep 2024

Abstract

The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) aims to prevent the overexploitation of species by controlling their trade. However, there is currently no international regulatory framework to protect the trade of non-CITES species. We examined the [...] Read more.

The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) aims to prevent the overexploitation of species by controlling their trade. However, there is currently no international regulatory framework to protect the trade of non-CITES species. We examined the LEMIS database, online trade, and scientific literature with the aim of identifying and compiling a list of South African native species traded as pets and wildlife products. We found that there are 223 non-CITES species traded as wildlife products and 95 species traded as pets. Mammals and birds were the most traded taxa for wildlife products, while reptiles and amphibians were mostly traded as pets. At the least, species traded as wildlife products and pets are currently not facing extinction, as most are categorized as Least Concern. However, some endemic species have an unknown population size, with Sclerophrys pantherina and Neamblysomus gunningi being Endangered. The international pet trade involves 10 countries, with the USA, the Czech Republic, and the UK being the largest importers. The trade of species as wildlife products involves 20 countries, with the USA being the major importer. This study emphasizes the necessity of strict regulations and international cooperation to control the wildlife trade effectively. Full article

(This article belongs to the Special Issue Wildlife Trade: Threats, Challenges, and Strategies for Prevention of Illegal Trade)

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18 pages, 3118 KiB

Open AccessArticle

Location, Age, and Antibodies Predict Avian Influenza Virus Shedding in Ring-Billed and Franklin’s Gulls in Minnesota

by Matthew Michalska-Smith, Eva Clements, Elizabeth Rasmussen, Marie R. Culhane and Meggan E. Craft

Animals 2024, 14(19), 2781; https://doi.org/10.3390/ani14192781 - 26 Sep 2024

Abstract

Influenza A virus (IAV) is a multi-host pathogen maintained in water birds and capable of spillover into humans, wildlife, and livestock. Prior research has focused on dabbling ducks as a known IAV reservoir species, yet our understanding of influenza dynamics in other water [...] Read more.

Influenza A virus (IAV) is a multi-host pathogen maintained in water birds and capable of spillover into humans, wildlife, and livestock. Prior research has focused on dabbling ducks as a known IAV reservoir species, yet our understanding of influenza dynamics in other water birds, including gulls, is lacking. Here, we quantify morphological and environmental drivers of serological (antibody detection by ELISA) and virological (viral RNA detection by PCR) prevalence in two gull species: ring-billed (Larus delawarensis) and Franklin’s (Leucophaeus pipixcan) gulls. Across 12 months and 10 locations, we tested over 1500 gulls for influenza viral RNA, and additionally tested antibody levels in nearly 1000 of these. We find substantial virus prevalence and a large, nonoverlapping seroprevalence, with significant differences across age and species classifications. The body condition index had minimal explanatory power to predict (sero)positivity, and the effect of the surrounding environment was idiosyncratic. Our results hint at a nontrivial relationship between virus and seropositivity, highlighting serological surveillance as a valuable counterpoint to PCR. By providing indication of both past infections and susceptibility to future infections, serosurveillance can help inform the distribution of limited resources to maximize surveillance effectiveness for a disease of high human, wildlife, and livestock concern. Full article

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14 pages, 1280 KiB

Open AccessArticle

Insight into the Gut–Brain Axis and the Productive Performance and Egg Quality Response to Kudzu Leaf Flavonoid Supplementation in Late-Laying Hens

by Shi Tang, Yaodong Hu, Jiahui Luo, Meijun Hu, Maolin Chen, Dehan Ye, Jingsong Ye and Fuguang Xue

Animals 2024, 14(19), 2780; https://doi.org/10.3390/ani14192780 - 26 Sep 2024

Abstract

(1) Background: Improving feed efficiency and the vitality of the reproductive system in the late stage of the egg-laying period is of great significance for prolonging the egg-laying cycle and improving egg quality. In the present study, a new flavonoid, which was extracted [...] Read more.

(1) Background: Improving feed efficiency and the vitality of the reproductive system in the late stage of the egg-laying period is of great significance for prolonging the egg-laying cycle and improving egg quality. In the present study, a new flavonoid, which was extracted from kudzu leaf, was chosen to investigate its effects on the productive performance and egg quality of late-laying hens. (2) Methods: A total of 360 500-day-old Hy-Line Brown layer hens were randomly divided into a control treatment group (no KLF supplementation), and groups that received 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% KLF supplement treatments. Each treatment contained 6 replicates, with 10 hens in each replicate. Productive performance metrics, including the daily egg production, egg weight, the number of deformed eggs, egg quality, egg density, egg shape index, eggshell strength, yolk color, and the Haugh unit, were meticulously recorded for each replicate. Furthermore, microbial communities and hypothalamus gene expressions were investigated based on the results of the productive performance and egg quality. (3) Results: KLF supplementation significantly decreased the deformity rate while significantly increasing the eggshell strength in the finishing phase afterward (p < 0.05). Specifically, hens supplemented with 0.6% KLF possessed the lowest deformed egg rate. KLF supplementation significantly increased the relative abundances of Bifidobacterium sp., Blautia sp., Lactococcus sp., and Lactobacillus sp., while significantly decreasing Parasutterella sp. and Escherichia-Shigella sp. (p < 0.05). Furthermore, the interactive analysis showed the hypothalamus gene expression mainly interacted with probiotics, such as Bifidobacterium sp. and Lactobacillus sp., through ribosome biogenesis, nucleocytoplasmic transport, and cAMP signaling pathways. (4) Conclusions: The findings of the present study indicate that KLF supplementation significantly proliferated probiotics, such as Bifidobacterium and Lactobacillus, which may have further interacted with hypothalamus genes, thus decreasing the deformity rate while increasing eggshell strength in the finishing phase. Full article

(This article belongs to the Special Issue Herbal Extracts and Herbal-Derived Products to Support Animal Health and Productivity)

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20 pages, 5605 KiB

Open AccessArticle

Medium- and Long-Term Immune Responses in the Small Intestine in Piglets from Oral Vaccination against Escherichia coli

by Aida Miralles, Guillermo Ramis, Francisco J. Pallarés, Ester Párraga-Ros and Juan Seva

Animals 2024, 14(19), 2779; https://doi.org/10.3390/ani14192779 - 26 Sep 2024

Abstract

Post-weaning stress, together with Escherichia coli, are two of the key factors in the occurrence of post-weaning diarrhea. There are different commercial vaccines that induce immunity at the local or systemic level, improving farm health and avoiding economic losses in the pork [...] Read more.

Post-weaning stress, together with Escherichia coli, are two of the key factors in the occurrence of post-weaning diarrhea. There are different commercial vaccines that induce immunity at the local or systemic level, improving farm health and avoiding economic losses in the pork industry. That is why the objective of this study was to evaluate the effect of an oral enterotoxigenic E. coli F4/F18 vaccine on immunity and intestinal integrity in the middle and long term after inoculation. The gene expression of the biomarkers indicative of cellular infiltration (calprotectin, CAL), tight junction proteins (occludin, OCL; zonulin, ZON; and claudin, CLA) and a panel of proinflammatory (interleukins, IL: IL1α, IL1β, IL6, IL8, IL12p35 and IL12p40; interferons, IFN: IFNα and IFNγ; and tumoral necrosis factor, TNF: TNFα) and anti-inflammatory mediator cytokines (TGFβ and IL10) were analyzed, as well as histomorphology in jejunum and ileum, the cell density of goblet cells, intraepithelial lymphocytes and IgA-producing cells. Differences were observed in ZON, CLA and CAL, with greater gene expression in observed in vaccinated piglets at 42 days post vaccination (dpv) in the ileum. Regarding the expression of cytokines, the vaccinated animals showed significant differences in IL1α, IL6, IL12p35, IL12p40, IFNα, IFNγ, TNFα and TGFβ at 42 dpv in the jejunum or ileum. The villi showed greater height in the vaccinated piglets and the ratio between villus height and crypt depth was significantly greater in the vaccinated group in the jejunum at 84 dpv. The count of IgA-producing cells shows higher values for the unvaccinated group in the ileum, while intraepithelial lymphocytes show a significant increase in both jejunum and ileum in vaccinated piglets. We can conclude that oral vaccination against E. coli produces an evident effect, which manifests itself even in the middle and long term after the challenge, including immune response, decrease in antimicrobials usage, better histological structure in intestine and the improvement of performance. Full article

(This article belongs to the Special Issue Gastrointestinal Tract Health in Pigs – 2nd Edition)

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24 pages, 484 KiB

Open AccessArticle

Nutrient Digestive Bypass: Determinants and Associations with Stool Quality in Cats and Dogs

by Matthew I. Jackson, Susan M. Wernimont, Kristen Carnagey and Dennis E. Jewell

Animals 2024, 14(19), 2778; https://doi.org/10.3390/ani14192778 - 26 Sep 2024

Abstract

The effect of digestive bypass macronutrients and age on stool quality (moisture and firmness) in dogs and cats is not well understood. Data were analyzed from digestibility tests (n = 2020, 361 dogs and 536 cats) including dry and wet product types. [...] Read more.

The effect of digestive bypass macronutrients and age on stool quality (moisture and firmness) in dogs and cats is not well understood. Data were analyzed from digestibility tests (n = 2020, 361 dogs and 536 cats) including dry and wet product types. Both food and feces were measured for moisture and nutrients according to standard protocols; stool firmness was graded. Linear mixed modeling was used to evaluate the associations between nutrient bypass, age and stool quality. Bypass protein increased stool moisture (dog, cat p < 0.0001) and decreased firmness (dog p = 0.01, cat p < 0.0001), while bypass fiber decreased stool moisture and increased firmness (dog, cat p < 0.0001 for both). Both species manifested a negative quadratic effect of advanced age on stool firmness (dog p < 0.0001 and cat p = 0.02). However, the association of advanced age (quadratic effect) with metabolizable energy required to maintain body weight was different between species; dogs had a positive association (p = 0.028), while it was negative for cats (p < 0.0001). Taken together, these data may aid in the development of food formulations for companion animals, which can better meet changing nutritional needs across life stages. Full article

(This article belongs to the Section Animal Nutrition)

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21 pages, 10375 KiB

Open AccessArticle

Sturnidae sensu lato Mitogenomics: Novel Insights into Codon Aversion, Selection, and Phylogeny

by Shiyun Han, Hengwu Ding, Hui Peng, Chenwei Dai, Sijia Zhang, Jianke Yang, Jinming Gao and Xianzhao Kan

Animals 2024, 14(19), 2777; https://doi.org/10.3390/ani14192777 - 26 Sep 2024

Abstract

The Sturnidae family comprises 123 recognized species in 35 genera. The taxa Mimidae and Buphagidae were formerly treated as subfamilies within Sturnidae. The phylogenetic relationships among the Sturnidae and related taxa (Sturnidae sensu lato) remain unresolved due to high rates of morphological [...] Read more.

The Sturnidae family comprises 123 recognized species in 35 genera. The taxa Mimidae and Buphagidae were formerly treated as subfamilies within Sturnidae. The phylogenetic relationships among the Sturnidae and related taxa (Sturnidae sensu lato) remain unresolved due to high rates of morphological change and concomitant morphological homoplasy. This study presents five new mitogenomes of Sturnidae sensu lato and comprehensive mitogenomic analyses. The investigated mitogenomes exhibit an identical gene composition of 37 genes—including 13 protein-coding genes (PCGs), 2 rRNA genes, and 22 tRNA genes—and one control region (CR). The most important finding of this study is drawn from CAM analyses. The surprisingly unique motifs for each species provide a new direction for the molecular species identification of avian. Furthermore, the pervasiveness of the natural selection of PCGs is found in all examined species when analyzing their nucleotide composition and codon usage. We also determine the structures of mt-tRNA, mt-rRNA, and CR structures of Sturnidae sensu lato. Lastly, our phylogenetic analyses not only well support the monophyly of Sturnidae, Mimidae, and Buphagidae, but also define nine stable subclades. Taken together, our findings will enable the further elucidation of the evolutionary relationships within Sturnidae sensu lato. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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17 pages, 20655 KiB

Open AccessArticle

Dietary Isatidis Root Residue Improves Diarrhea and Intestinal Function in Weaned Piglets

by Zhong Chen, Zenghao Yan, Siting Xia, Kaijun Wang, Qi Han, Miao Zhou, Deqin Wang, Jie Yin and Yulong Yin

Animals 2024, 14(19), 2776; https://doi.org/10.3390/ani14192776 - 26 Sep 2024

Abstract

Weaning stress can trigger diarrhea, cause intestinal damage, and disrupt the intestinal flora of piglets, ultimately resulting in retarded growth or even the death of the animals. Traditional Chinese medicine residues encompass numerous bioactive compounds and essential nutrients; however, their efficient utilization remains [...] Read more.

Weaning stress can trigger diarrhea, cause intestinal damage, and disrupt the intestinal flora of piglets, ultimately resulting in retarded growth or even the death of the animals. Traditional Chinese medicine residues encompass numerous bioactive compounds and essential nutrients; however, their efficient utilization remains a challenge. Consequently, our study sought to explore the impact of traditional Chinese medicine residues, specifically Isatidis Root residue (IRR), on the growth performance, intestinal function, and occurrence of weaning diarrhea in newly weaned piglets. Forty healthy, castrated Duroc × Landrace × Yorkshire males, weaned at 21 days old and exhibiting similar body conditions, were randomly allocated into five groups, with eight piglets in each group. The results indicated that the dietary inclusion of IRR at concentrations ranging from 0.5% to 4.0% notably decreased the incidence of diarrhea in weaned piglets compared to the control group (p < 0.05). Serum LDL-C and globulin (GLB) contents were reduced in response to dietary IRR concentrations (0.5% to 4.0%), while serum albumin (ALB) and albumin/globulin (A/G) contents were enhanced (p < 0.05). Dietary 0.5%, 1.0%, and 2.0% IRR resulted in significant increases in villus height (VH) and villus height/crypt depth (V/C) ratios in the jejunum, V/C ratios in the ileum, and the number of villi goblet cells both in the jejunum and ileum. IRR also led to a significant decrease in the crypt depth (CD) of the jejunum and ileum (p < 0.05). Furthermore, the expression of IL-6 in the jejunum was significantly increased in IRR-fed piglets (0.5% to 4.0%) (p < 0.05). IRR demonstrated inhibitory effects on harmful bacteria in the gastrointestinal microbiome, including Campylobacter, Actinobacillus minor, and Ralstonia pickettii, indicating its broad-spectrum bacteriostatic properties. In conclusion, dietary IRR alleviated diarrhea in weaned piglets and improved gut function and microbial compositions. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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13 pages, 1172 KiB

Open AccessCase Report

Managing a Salmonella Bredeney Outbreak on an Italian Dairy Farm

by Camilla Torreggiani, Cosimo Paladini, Marcello Cannistrà, Benedetta Botti, Alice Prosperi, Chiara Chiapponi, Laura Soliani, Ada Mescoli and Andrea Luppi

Animals 2024, 14(19), 2775; https://doi.org/10.3390/ani14192775 - 26 Sep 2024

Abstract

Salmonellosis in dairy cattle represents an increasing problem for both animal and public health. Nevertheless, in Italy, there is no control plan in place on dairy farms. The aim of this study was to describe a Salmonella Bredeney outbreak that occurred on a [...] Read more.

Salmonellosis in dairy cattle represents an increasing problem for both animal and public health. Nevertheless, in Italy, there is no control plan in place on dairy farms. The aim of this study was to describe a Salmonella Bredeney outbreak that occurred on a dairy farm and the measures that were adopted to control the outbreak. Management consisted in identifying the spread of infection and assessing the environmental contamination of Salmonella spp. and the associated risk factors. After the farm visit, laboratory investigations showed that 48% of rectal swabs collected from calves and 33% of environmental samples were positive for S. Bredeney, and a poor biosecurity level was detected. The farmer and practitioner were provided with a health management plan to control the spread of Salmonella spp., followed by a monitoring period and a follow-up visit in which all samples resulted negative. The results demonstrated the efficacy of indirect prophylaxis measures in reducing the circulation of Salmonella spp., leading to the extinction of the outbreak. Collaboration with farmers, practitioners, and public health veterinarians and the introduction of measures reported in the health management plan constitute a possible model for the management of Salmonella spp. outbreaks in dairy herds, even in complex farm situations. Full article

(This article belongs to the Section Veterinary Clinical Studies)

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20 pages, 2107 KiB

Open AccessFeature PaperArticle

The Effects of Onychectomy (Declawing) on Forearm and Leg Myology in a Kinkajou (Potos flavus)

by Lara L. Martens, Reece A. Brown, Ana Carolina Lourenço Faillace, Arin Berger, Rachel L. J. Smith, Kathryn Bertok, Lauren Humphries, Angela Lassiter and Adam Hartstone-Rose

Animals 2024, 14(19), 2774; https://doi.org/10.3390/ani14192774 - 26 Sep 2024

Abstract

Recently, onychectomy, the “declaw” surgery in which all or part of the distal phalanges are removed, has been shown to have significant effects on the forearm muscles of felids. While this surgery should clearly affect the limb muscles (especially those that insert on [...] Read more.

Recently, onychectomy, the “declaw” surgery in which all or part of the distal phalanges are removed, has been shown to have significant effects on the forearm muscles of felids. While this surgery should clearly affect the limb muscles (especially those that insert on the removed or modified bone), these effects have not been studied beyond felids or in the hindlimb. To that end, we herein evaluated the muscle architecture of a kinkajou (Potos flavus) that was declawed on all four of its limbs and compared its anatomy to that of intact specimens and the felid findings. As expected, some of the declawed kinkajou’s muscles were substantially different from those of the intact specimens, and as was seen in felids, its digital muscles appear to have been weaker. However, unlike in the felids, the declawed kinkajou had relatively larger forearm muscles. Also, contrary to expectation, the leg muscles of the declawed kinkajou were not substantially different, perhaps reflecting important differences in limb use. Future analyses should examine this anatomy in other declawed kinkajou specimens and also look at the effects of this surgery in other taxa, for instance, non-arboreal relatives of the kinkajou as well as other arboreal taxa. Full article

(This article belongs to the Section Veterinary Clinical Studies)

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14 pages, 486 KiB

Open AccessFeature PaperArticle

How Attachment to Dogs and to Other Humans Relate to Mental Health

by Katherine Northrope, Matthew B. Ruby and Tiffani J. Howell

Animals 2024, 14(19), 2773; https://doi.org/10.3390/ani14192773 - 26 Sep 2024

Abstract

It is unclear how pet ownership is related to mental health, with some previous research suggesting pet owners have better mental health, while other research suggests they have worse mental health. Some researchers have suggested that it may be more useful to investigate [...] Read more.

It is unclear how pet ownership is related to mental health, with some previous research suggesting pet owners have better mental health, while other research suggests they have worse mental health. Some researchers have suggested that it may be more useful to investigate the bond people feel with their pets and how this may impact mental health; however, this too has led to mixed results. This study examined how owners’ attachment to their dogs was associated with mental health and how this compared to their attachment relationships with other humans in a sample of 607 dog owners. Our findings indicate that both strong and insecure attachments to dogs are linked to poorer mental health outcomes, as was having an insecure attachment style in their human relationships. The adverse impact of strong attachment to dogs on mental health was mediated by these owners having an anxious attachment style toward other people, which in turn was associated with poorer mental health. The relationship between a strong attachment to dogs and poorer mental health was also moderated by gender, with this relationship being significant in women but not significant for men. Together, these results suggest a possible risk to mental health for owners who form a strong attachment to their dogs to compensate for anxious attachments in human relationships. Full article

(This article belongs to the Special Issue Second Edition: Research on the Human–Companion Animal Relationship)

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10 pages, 1525 KiB

Open AccessFeature PaperArticle

Characterisation of Ovine KRTAP19-3 and Its Impact on Wool Traits in Chinese Tan Sheep

by Lingrong Bai, Huitong Zhou, Jinzhong Tao and Jon G. H. Hickford

Animals 2024, 14(19), 2772; https://doi.org/10.3390/ani14192772 - 25 Sep 2024

Viewed by 187

Abstract

Wool, a natural fibre derived from sheep, can present a challenge to wool processing and manufacturing industries because of the variation in fibre traits. Genetic improvement offers one solution to this challenge, and having a better understanding of the genes that affect wool [...] Read more.

Wool, a natural fibre derived from sheep, can present a challenge to wool processing and manufacturing industries because of the variation in fibre traits. Genetic improvement offers one solution to this challenge, and having a better understanding of the genes that affect wool fibre traits is therefore important. Here, we describe ovine KRTAP19-3, a new member of the KAP19 gene family. Phylogenetic analysis revealed its relationship to other known KRTAP19 gene sequences, and an analysis of the nucleotide sequence variation in KRTAP19-3 from 288 sheep of a variety of breeds revealed six unique variant sequences. Among these variants, eleven single nucleotide polymorphisms (SNPs) were detected, with six located in the coding region. Three of these coding region SNPs were non-synonymous and would result in amino acid changes. Associations were observed between the presence of specific sequence variants in Chinese Tan sheep and wool trait variation, particularly an increase in fibre diameter variability in the heterotypic hair fibres. These findings enhance our understanding of the genes that encode sheep wool proteins. Full article

(This article belongs to the Special Issue Genetics and Breeding in Ruminants)

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18 pages, 6301 KiB

Open AccessArticle

Melatonin Regulates the Expression of VEGF and HOXA10 in Bovine Endometrial Epithelial Cells through the SIRT1/PI3K/AKT Pathway

by Qi Li, Ying Tang, Yanru Chen, Bo Li, Hongzhan Wang, Shicheng Liu, Samson O. Adeniran and Peng Zheng

Animals 2024, 14(19), 2771; https://doi.org/10.3390/ani14192771 - 25 Sep 2024

Viewed by 170

Abstract

Melatonin plays a critical role in regulating embryo attachment in ruminants. While numerous studies have investigated its effects on early embryo development in vitro, the precise mechanisms by which melatonin influences the receptivity of endometrial epithelial cells in dairy cows remain unclear. The [...] Read more.

Melatonin plays a critical role in regulating embryo attachment in ruminants. While numerous studies have investigated its effects on early embryo development in vitro, the precise mechanisms by which melatonin influences the receptivity of endometrial epithelial cells in dairy cows remain unclear. The prerequisite for embryo implantation is the specific physiological condition of the endometrium that allows the embryo to implant, also known as endometrial receptivity. In addition to this, endometrial cells undergo processes such as proliferation, differentiation, and renewal, which makes the embryo more easily implanted. In this study, bovine endometrial epithelial cells were cultured and treated with melatonin, Silent Information Regulator 1 (SIRT1) inhibitor (EX527), and protein kinase B (AKT) phosphorylation inhibitor (periposine). RT-qPCR, Western blot, and immunofluorescence analysis were performed to investigate the effects of melatonin on the expression of target gene (SIRT1); cell proliferative genes, phosphatidylinositol-4,5-bisphosphate 3-Kinase (PI3K), AKT, cyclinD1, cyclinE1; and receptive genes (Leukemia Inhibitory Factor (LIF), Vascular Endothelial Growth Factor (VEGF), Homeobox Structure Gene 10 (HOXA10)). Additionally, microRNA (miRNA) mimics and inhibitors were used to transfect the cells to study the regulatory relationship between miRNA and receptive genes. Results indicated that melatonin activates the PI3K/AKT signaling pathway, upregulates cyclinD1 and cyclinE1, and promotes the proliferation of bovine endometrial epithelial cells. Melatonin also upregulated the expression of VEGF and HOXA10 and downregulated the expression of bta-miR-497 and bta-miR-27a-3p through SIRT1/PI3K/AKT signaling pathway. Further, bta-miR-497 and bta-miR-27a-3p were found to negatively regulate VEGF and HOXA10, respectively. Therefore, melatonin regulates the expression of VEGF and HOXA10 through the SIRT1/PI3K/AKT pathway and promotes the establishment of receptivity in bovine endometrial epithelial cells. Full article

(This article belongs to the Section Animal Reproduction)

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22 pages, 7393 KiB

Open AccessFeature PaperArticle

Making Noah’s Ark Work for Fishing Cat Conservation: A Blueprint for Connecting Populations Across an Interactive Wild Ex Situ Spectrum

by Tiasa Adhya, Simran Singh, Himaja Varma Gottumukkala, Aditya Banerjee, Ishita Chongder, Sulata Maity and P. Anuradha Reddy

Animals 2024, 14(19), 2770; https://doi.org/10.3390/ani14192770 - 25 Sep 2024

Viewed by 399

Abstract

The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, [...] Read more.

The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, and emotional needs of captive individuals, thereby improving conservation outcomes. Here, we present an integrated framework for the conservation of a globally threatened wetland wild cat species, the fishing cat, by synthesizing optimal ex situ management practices and in situ conservation strategies. Further, we examined the genetic constitution of the founder population in a fishing cat captive breeding program that was recently initiated by the West Bengal Zoo Authority, India and conducted a population viability analysis to suggest how best to maintain the genetic diversity of the population. We found that the present genetic diversity of 56% and maximum carrying capacity of the captive population (30 individuals) can be maintained for more than 100 years with a combination of supplementation and harvesting. Keeping stochastic events in mind, the introduction of two adult males and females to the existing population each year will seamlessly allow the harvesting of two adult males and two adult females every alternate year to supplement wild populations. Further, we adopted the proposed integrated framework to delineate recommendations for the supplementation of wild populations in West Bengal. We used environmental criteria known to influence fishing cat occurrence to identify 21 potential reintroduction zones in the Sundarbans landscape and Terai region in northern West Bengal with habitable areas for the fishing cat that are larger than the maximum known species’ home range. Our study is timely and insightful because it provides a holistic blueprint for implementing the One Plan Approach in safeguarding a threatened species. Full article

(This article belongs to the Special Issue Zoo Animals: How Actual Zoological Institutions Assess, Ensure, and Promote Their Animals’ Welfare?)

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17 pages, 3042 KiB

Open AccessFeature PaperReview

Nuancing ‘Emotional’ Social Play: Does Play Behaviour Always Underlie a Positive Emotional State?

by Giada Cordoni and Ivan Norscia

Animals 2024, 14(19), 2769; https://doi.org/10.3390/ani14192769 - 25 Sep 2024

Viewed by 325

Abstract

This review focuses on social play, a complex behaviour that is often difficult to categorize. Although play has been typically associated with positive emotional states, a thorough examination of the literature indicates that it may relate to different emotional systems, from attachment to [...] Read more.

This review focuses on social play, a complex behaviour that is often difficult to categorize. Although play has been typically associated with positive emotional states, a thorough examination of the literature indicates that it may relate to different emotional systems, from attachment to conflict. Play oscillates between competition and cooperation, and includes a spectrum in between; thus, quantitatively identifying and demonstrating the emotional nature of play remains challenging. We considered examples from human and non-human animal studies and explored the emotional and neuro-hormonal systems involved in play. We assessed ethological data possibly indicating the emotional states underlying play, and we focused on the cooperative and competitive elements of play. We investigated the relationship between play and affiliative/aggressive behaviours, the communicative meaning of play signals (especially primate play faces), and the motor and possibly emotional contagion function of rapid motor mimicry during play. From all the literature on play, this review selects and combines studies in an innovative way to present the methods (e.g., play indices and social network analysis), tools (e.g., sequential analysis and facial coding software), and evidence indicative of the emotional states underlying play, which is much more complex than previously thought. Full article

(This article belongs to the Special Issue Emotional Contagion in Animals)

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16 pages, 1374 KiB

Open AccessFeature PaperArticle

Effect of Thawing Procedure and Thermo-Resistance Test on Sperm Motility and Kinematics Patterns in Two Bovine Breeds

by Juan M. Solís, Francisco Sevilla, Miguel A. Silvestre, Ignacio Araya-Zúñiga, Eduardo R. S. Roldan, Alejandro Saborío-Montero and Anthony Valverde

Animals 2024, 14(19), 2768; https://doi.org/10.3390/ani14192768 - 25 Sep 2024

Viewed by 189

Abstract

This investigation aimed to analyze the effect that thawing time and temperature in combination with a termo-resistance test had on straws from dairy bulls used for artificial insemination (AI) on semen motility and kinematic variables measured with CASA systems. Eight animals of Holstein [...] Read more.

This investigation aimed to analyze the effect that thawing time and temperature in combination with a termo-resistance test had on straws from dairy bulls used for artificial insemination (AI) on semen motility and kinematic variables measured with CASA systems. Eight animals of Holstein and Jersey breeds were used, and nine frozen-thawed semen doses per animal were analyzed for each breed. Three temperatures (35, 37, and 40 °C) and three thawing times (35, 40, and 45 s) were evaluated using a factorial design. Motility and kinematic patterns were analyzed using CASA-mot (Computer-Assisted Semen Analysis of motility) technology at different post-thawing times (0.5, 1, and 2 h). Sperm motility in Jersey bulls was higher (p < 0.05) than in Holstein ones (64.52 ± 1.45% and 53.10 ± 1.40%, respectively). The same effect was seen with progressive motility among the two breeds (Jersey: 45.29 ± 1.00%; Holstein: 36.30 ± 0.98%, p < 0.05). The Jersey breed presented higher values (p < 0.05) of curvilinear velocity (VCL), rectilinear velocity (VSL), average velocity (VAP), linearity on forward progression (LIN), and wobble (WOB). The Holstein breed showed a lower mean value (p < 0.05) of the beat-cross frequency (BCF) compared to the Jersey breed, thus suggesting an effect on VCL and VAP. During the post-thaw period, a gradual increase in VCL was observed at 2 h. VSL and VAP showed a decrease (p < 0.05) as the post-thaw period was prolonged. The study showed differences in sperm quality between Holstein and Jersey breeds, influenced by cryopreservation, thawing, and post-thawing incubation. Thawing at 37 °C for 30 s was considered optimal in relation to sperm motility. In addition, a decrease in sperm quality was observed as post-thawing time increased. Full article

(This article belongs to the Special Issue Technological Applications in Farm Animal Reproduction)

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16 pages, 6757 KiB

Open AccessArticle

Transcriptomic Study of Different Stages of Development in the Testis of Sheep

by Binpeng Xi, Shengguo Zhao, Rui Zhang, Zengkui Lu, Jianye Li, Xuejiao An and Yaojing Yue

Animals 2024, 14(19), 2767; https://doi.org/10.3390/ani14192767 - 25 Sep 2024

Viewed by 173

Abstract

Numerous genes govern male reproduction, modulating testicular development and spermatogenesis. Our study leveraged RNA-Seq to explore candidate genes and pivotal pathways influencing fecundity in an F1 hybrid of Southdown × Hu sheep testes across four developmental milestones: M0 (0 months old, newborn), M3 [...] Read more.

Numerous genes govern male reproduction, modulating testicular development and spermatogenesis. Our study leveraged RNA-Seq to explore candidate genes and pivotal pathways influencing fecundity in an F1 hybrid of Southdown × Hu sheep testes across four developmental milestones: M0 (0 months old, newborn), M3 (3 months old, sexually immature), M6 (6 months old, sexually mature), and Y1 (1 years old, adult). Histological examination using hematoxylins and eosin staining revealed that the cross-sectional area of the spermatid tubules and the number of supportive cells increased in the other groups, as compared to the M0 group. The cross-sectional area of the vasculature and the number of supporting cells were found to be significantly increased in all other groups in comparison to the M0 group. We conducted GO and KEGG analyses of the differentially expressed genes (DEGs) in the three comparison groups and identified key pathways, including cAMP, MAPK, ECM–receptor interactions, PI3K-Akt, and FOXO signaling, which are closely related to testicular development and spermatogenesis. Notably, alternative splicing (AS) events were markedly elevated in M6 and Y1 stages. Key genes like GATA4, GATA6, SMAD4, SOX9, YAP1, ITGB1 and MAPK1 emerged as significantly enriched in these pathways, potentially orchestrating the transition from immature to mature testes in sheep. These findings offer valuable insights into male reproductive potential and can inform strategies for optimizing animal breeding. Full article

(This article belongs to the Section Animal Reproduction)

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13 pages, 2240 KiB

Open AccessArticle

Association of Encephalitozoon cuniculi with Clinical Signs and Abnormal Hematologic/Biochemical Changes in Pet Rabbits in Thailand

by Taksaon Duangurai, Natruree Khamchomphu, Kanyanut Dusitkul, Chawaporn Tousee, Yosanun Sukmai, Teerapat Rungnirundorn, Ladawan Areevijittrakul, Siriluk Jala and Naris Thengchaisri

Animals 2024, 14(19), 2766; https://doi.org/10.3390/ani14192766 - 25 Sep 2024

Viewed by 248

Abstract

Encephalitozoon cuniculi can cause serious disease and subclinical infection in rabbits and requires active surveillance to control the infection. This study investigated the association between anti-Encephalitozoon cuniculi antibody status and various health parameters in pet rabbits. A total of 90 rabbits were [...] Read more.

Encephalitozoon cuniculi can cause serious disease and subclinical infection in rabbits and requires active surveillance to control the infection. This study investigated the association between anti-Encephalitozoon cuniculi antibody status and various health parameters in pet rabbits. A total of 90 rabbits were divided into healthy (N = 30), subclinical (N = 30), and clinical (N = 30) groups based on their anti-Encephalitozoon cuniculi antibody status and clinical presentations. The mean ages of the control (37 ± 40 months) and subclinical groups (38 ± 34 months) were notably lower compared to that of the clinical group (63 ± 38 months, p < 0.01). Serum titers for anti-Encephalitozoon cuniculi antibodies were significantly elevated in rabbits with subclinical and clinical infections compared to those of healthy rabbits (p < 0.05). Neurological signs were predominant in rabbits with active E. cuniculi infection (80.0%), with additional pathological features including urinary dysfunction (10.0%) and cataracts (10.0%). The source of rabbits was not associated with E. cuniculi infection (p = 0.159). Anemia was significantly linked to E. cuniculi infection (p = 0.026); however, no significant associations were found with leukocytosis, thrombocytopenia, or serum biochemistry parameters. Mature adult rabbits were more likely to be infected with E. cuniculi. Recognizing anemia and neurological signs facilitates in early diagnosis of E. cuniculi infection. Full article

(This article belongs to the Special Issue Exotic Mammal Care and Medicine)

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21 pages, 7684 KiB

Open AccessFeature PaperReview

A Meta-Analysis on Quantitative Calcium, Phosphorus and Magnesium Metabolism in Horses and Ponies

by Isabelle Maier and Ellen Kienzle

Animals 2024, 14(19), 2765; https://doi.org/10.3390/ani14192765 - 25 Sep 2024

Viewed by 294

Abstract

The aims of the present meta-analysis were (i) to re-evaluate the factorially calculated Ca, P and Mg requirements to replace endogenous faecal losses, taking new data into account, (ii) to identify potential differences between horses and ponies regarding requirements, apparent digestibility, serum levels [...] Read more.

The aims of the present meta-analysis were (i) to re-evaluate the factorially calculated Ca, P and Mg requirements to replace endogenous faecal losses, taking new data into account, (ii) to identify potential differences between horses and ponies regarding requirements, apparent digestibility, serum levels and renal excretion of Ca, P and Mg and (iii) to investigate the influence of mineral sources, i.e., “inorganic” sources from added mineral salts and “organic” sources from feed plants. For P, the water solubility of “inorganic” sources was taken into consideration. Data on the aforementioned parameters from 42 studies were plotted against intake, similar to the Lucas test for true digestibility and faecal endogenous losses. Within specific intake ranges, data were compared using t-tests and an ANOVA, followed by Holm–Sidak post hoc tests. Ponies had lower endogenous faecal Mg losses than horses. Consequently, apparent Mg digestibility was higher in ponies. Factorial calculations of Mg requirements to replace faecal losses showed that ponies needed approximately half of the current recommended amount, while horses required 1.9 times the amount currently recommended by Kienzle and Burger. The overall mean matched previous recommendations. For Ca, there was no discernible difference between ponies and horses. True Ca digestibility calculated by the Lucas test was higher and endogenous losses were lower when “organic” Ca was fed as opposed to when “inorganic” sources were used. The resulting factorial calculations of the requirements to replace faecal losses were close to current recommendations for “organic” Ca. For “inorganic” sources, however, the new calculations were below the recommended level. For P, there were no discernible differences between horses and ponies. There were also no clear effects of “inorganic” or “organic” P sources. The water solubility of “inorganic” sources was the key factor determining P metabolism. Water-soluble P sources exhibited higher true and apparent digestibility. The intake of these P sources led to hyperphosphatemia and hyperphosphaturia, even at low intakes. In other species, this has been shown to pose a health risk. Therefore, it is recommended to avoid the use of highly water-soluble “inorganic” P sources in horses and ponies. Given the lower digestibility of insoluble P sources, the factorially calculated P requirements for such sources are higher than the current recommendations. Full article

(This article belongs to the Section Animal Nutrition)

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14 pages, 705 KiB

Open AccessArticle

Determination and Prediction of Amino Acid Digestibility in Rapeseed Cake for Growing-Finishing Pigs

by Hui Tang, Ganyi Feng, Jingfeng Zhao, Qing Ouyang, Xiaojie Liu, Xianji Jiang, Menglong Deng, Zhengjun Xie, Fengming Chen, Xihong Zhou, Rui Li and Yulong Yin

Animals 2024, 14(19), 2764; https://doi.org/10.3390/ani14192764 - 25 Sep 2024

Viewed by 236

Abstract

Objective: The experiment was conducted to determine the apparent or standardized ileal digestibility (AID or SID) of crude protein (CP) and amino acids (AA) in 10 rapeseed cake samples fed to pigs, and to construct predictive models for the SID of CP and [...] Read more.

Objective: The experiment was conducted to determine the apparent or standardized ileal digestibility (AID or SID) of crude protein (CP) and amino acids (AA) in 10 rapeseed cake samples fed to pigs, and to construct predictive models for the SID of CP and AA based on the chemical composition of rapeseed cakes. Methods: Twenty-two cannulated pigs (initial body weight: 39.8 ± 1.2 kg) were assigned to two 11 × 3 incomplete Latin square designs, including an N-free diet and 10 diets containing rapeseed cake. Each experimental period included 5 days of adaptation and 2 days of ileal digesta collection. Titanium dioxide (TiO₂) was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results: The coefficients of variation (CV) of the content of crude fat (EE), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), and total glucosinolates (TGS) in 10 samples of rapeseed cake were greater than 10%. The standardized ileal digestibility (SID) of crude protein (CP), lysine (Lys), methionine (Met), threonine (Thr), and tryptophan (Trp) in rapeseed cake was 73.34% (61.49 to 81.12%), 63.01% (41.41 to 73.10%), 69.47% (50.55 to 88.16%), 79.61% (74.41 to 87.58%), and 94.43% (91.34 to 97.20%), respectively. The best prediction equations for SID_CP, SID_Lys, and SID_Val were as follows: SID_CP = 90.124 − 0.54NDF (R² = 0.58), SID_Lys = 100.107 − 1.229NDF (R² = 0.94), and SID_Val = 151.012 − 2.990TGS (R² = 0.57). Conclusion: Overall, great variation exists among the 10 rapeseed cakes, and the NDF, TGS, and heating temperature can be used as the key predictors for the SID of CP and AA. Full article

(This article belongs to the Special Issue Exploration of Sustainable Feed Resources and Pig Dietary Strategies)

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17 pages, 303 KiB

Open AccessArticle

Amino Acid Content in the Muscles of the Red Deer (Cervus elaphus) from Three Types of Feeding Grounds

by Anna Kasprzyk

Animals 2024, 14(19), 2763; https://doi.org/10.3390/ani14192763 - 25 Sep 2024

Viewed by 275

Abstract

This study aimed to analyze the amino acid profile, with a particular focus on the nutritional value of the protein of the longissimus lumborum (LL) and the semimembranosus (SM) muscles of deer originating from three feeding grounds: forest (FFG); conventional (CFG) grounds; organic [...] Read more.

This study aimed to analyze the amino acid profile, with a particular focus on the nutritional value of the protein of the longissimus lumborum (LL) and the semimembranosus (SM) muscles of deer originating from three feeding grounds: forest (FFG); conventional (CFG) grounds; organic farm (OFG). This is the first time that deer from an organic farm feeding ground have been included in this study. The muscles were collected from 36 deer carcasses with equal proportions of sex and 31 months of age. This study demonstrated significantly higher essential amino acid (EAA) and non-essential amino acid (NEAA) contents in the muscles of deer from the FFG and CFG compared to the OFG. However, the EAA-to-NEAA ratio was significantly higher for the muscles of deer from the OFG. The muscles of the FFG and CFG deer were characterized by a higher concentration of lysine as well as acidic and tasty amino acids compared to the OFG deer, with the muscles of the latter exhibiting a higher percentage of branched-chain amino acids (BCAA). The results obtained can be used professionally by nutrition specialists in preventive and therapeutic diets and breeders to make decisions about farm location and deer feeding strategies. Full article

(This article belongs to the Special Issue Recent Advances in Milk and Meat Products—the Influence of Animal Breed, Nutrition and Growth Conditions)

12 pages, 2333 KiB

Open AccessCase Report

Rescue, Rehabilitation, and Feeding Schedule of a Franciscana Dolphin (Pontoporia blainvillei) Calf Stranded in Argentina

by Julio Daniel Loureiro, Juan Pablo Loureiro, Sergio Rodriguez Heredia, Lorenzo von Fersen, Karina Cecilia Alvarez, María Julieta Olocco Diz and Romina Nuñez Favre

Animals 2024, 14(19), 2762; https://doi.org/10.3390/ani14192762 - 24 Sep 2024

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Abstract

The franciscana dolphin (Pontoporia blainvillei) is a small cetacean endemic to the coastal waters of the southwestern Atlantic Ocean. Due to its restricted distribution, it is subject to high bycatch mortality in the gillnets used for commercial and artisanal fishing. The [...] Read more.

The franciscana dolphin (Pontoporia blainvillei) is a small cetacean endemic to the coastal waters of the southwestern Atlantic Ocean. Due to its restricted distribution, it is subject to high bycatch mortality in the gillnets used for commercial and artisanal fishing. The rehabilitation of the franciscana is still a major challenge, as most attempts to rehabilitate stranded animals have failed. This study aims to present the case of the rehabilitation of a franciscana dolphin calf, stranded in San Clemente del Tuyú, Argentina, at the beginning of the predation period. The feeding strategy and nutritional profile at different stages during the 88 days of rehabilitation are meticulously detailed. Its diet was prepared by hand based on studies of milk composition, the feeding ecology of franciscana in Argentinean waters, and previous records of the Fundación Mundo Marino Rehabilitation Center. The diets were designed to meet the nutritional needs of franciscana dolphins. In addition, the hematological, cytological, and fecal analyses recorded during the rehabilitation are presented. Although the animal could not be released, due to its death, this report provides baseline information that can improve the ability of veterinarians to care for debilitated, live-stranded dolphins. This information may also be useful in the implementation and development of healthcare protocols for this species. Full article

(This article belongs to the Section Aquatic Animals)

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Animals, Volume 14, Issue 19 (October-1 2024) – 38 articles

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