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Open AccessArticle
Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community
by
Haitao Liu
Haitao Liu 1,2,3,
Fei Li
Fei Li 1,2,3,
Zhiyuan Ma
Zhiyuan Ma 1,2,3,*,
Miaomiao Ma
Miaomiao Ma 4,
Emilio Ungerfeld
Emilio Ungerfeld 5,
Zhian Zhang
Zhian Zhang 1,2,3,
Xiuxiu Weng
Xiuxiu Weng 1,2,3,
Baocang Liu
Baocang Liu 6,
Xiaoyu Deng
Xiaoyu Deng 6 and
Liqing Guo
Liqing Guo 7
1
State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China
2
College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
3
Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou 730020, China
4
Animal Husbandry Work Station of Ningxia, Yingchuan 750002, China
5
Centro Regional de Investigación Carillanca, Instituto de Investigaciones Agropecuarias INIA, Vilcún 4880000, Chile
6
Aksu Tycoon Feed Co., Ltd., Aksu 843000, China
7
Tecon Pharmaceutical Co., Ltd., Suzhou 215000, China
*
Author to whom correspondence should be addressed.
Submission received: 13 September 2024
/
Revised: 7 October 2024
/
Accepted: 8 October 2024
/
Published: 9 October 2024
Simple Summary
This study evaluated the influence of four active dry yeasts (ADYs) on nutrient digestibility and rumen fermentation in lambs through in vitro and in vivo experiments. Notably, Vistacell (Vis) and Procreatin7 (Pro) showed enhanced total gas production and pH levels in vitro. Vis improved in vivo propionate molar proportion, NDF digestibility, and total VFA concentration, suggesting it is the most suitable for lamb growth. However, the study highlighted discrepancies between in vitro and in vivo outcomes, cautioning against directly translating batch culture results to live animal effects.
Abstract
This study assessed the impact of active dry yeast(ADY) on nutrient digestibility and rumen fermentation, using both in vitro and in vivo experiments with lambs. In vitro, ADYs were incubated with rumen fluid and a substrate mixture to assess gas production, pH, volatile fatty acid(VFA) profiles, and lactate concentration. In vivo, Hu lambs were randomly assigned to five dietary treatments: a control group and four groups receiving one of two dosages of either Vistacell or Procreatin7. Growth performance, nutrient digestibility, rumen fermentation parameters, and bacterial community composition were measured. Pro enhanced the propionate molar proportion while it decreased the n-butyrate molar proportion. Vis reduced the lactate concentration in vitro. In the in vivo experiment, Vis increased the propionate molar proportion and the Succinivibrionaceae_UCG-001 abundance while it decreased the n-butyrate molar proportion and the Lachnospiraceae_ND3007 abundance. Additionally, Vis showed a greater impact on improving the NDF digestibility and total VFA concentration in vivo compared to Pro. Overall, the effects of ADYs on rumen fermentation were found to vary depending on the specific ADY used, with Vis being the most suitable for lamb growth. It was observed that Vis promoted propionate fermentation and Succinivibrionaceae_UCG-001 abundance at the expense of reduced n-butyrate fermentation and Lachnospiraceae_ND3007 abundance. Importantly, differences were noted between the outcomes of the in vitro and in vivo experiments concerning the effects of ADYs on rumen fermentation, highlighting the need for caution when generalizing batch culture results to the in vivo effects of ADYs.
Share and Cite
MDPI and ACS Style
Liu, H.; Li, F.; Ma, Z.; Ma, M.; Ungerfeld, E.; Zhang, Z.; Weng, X.; Liu, B.; Deng, X.; Guo, L.
Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community. Animals 2024, 14, 2916.
https://doi.org/10.3390/ani14192916
AMA Style
Liu H, Li F, Ma Z, Ma M, Ungerfeld E, Zhang Z, Weng X, Liu B, Deng X, Guo L.
Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community. Animals. 2024; 14(19):2916.
https://doi.org/10.3390/ani14192916
Chicago/Turabian Style
Liu, Haitao, Fei Li, Zhiyuan Ma, Miaomiao Ma, Emilio Ungerfeld, Zhian Zhang, Xiuxiu Weng, Baocang Liu, Xiaoyu Deng, and Liqing Guo.
2024. "Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community" Animals 14, no. 19: 2916.
https://doi.org/10.3390/ani14192916
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