Occurrence of Eimeria spp. and Intestinal Helminths in Free-Range Chickens from Northwest and Central Romania
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals, Samples, and Sample Analysis
2.2. PCR
2.3. Statistical Analysis
2.4. Ethical Statement
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Primer Sequence 5′ 3′ | Annealing Temperature (°C) | Amplicon Size (bp) |
---|---|---|---|
E. acervulina | F 5′-GGGCTTGGATGATGTTTGCTG-3′ R 5′-GCAATGATGCTTGCACAGTCAGG-3′ | 65 | 145 |
E. brunetti | F 5′-CTGGGGCTGCAGCGACAGGG-3′ R 5′-ATCGATGGCCCCATCCCGCAT-3′ | 58 | 183 |
E. maxima | F 5′-GTGGGACTGTGGTGATGGGG-3′ R 5′-ACCAGCATGCGCTCACAACCC-3′ | 65 | 205 |
E. mitis | F 5′-GTTTATTTCCTGTCGTCGTCTCGC-3′ R 5′-GTATGCAAGAGAGAATCGGGATTCC-3′ | 65 | 330 |
E. necatrix | F 5′-AGTATGGGCGTGAGCATGGAG-3′ R 5′-GATCAGTCTCATCATAATTCTCGCG-3′ | 58 | 160 |
E. praecox | F 5′-CATCGGAATGGCTTTTTGAAAGCG-3′ R 5′-GCATGCGCTAACAACTCCCCTT-3′ | 65 | 215 |
E. tenella | F 5′-AATTTAGTCCATCGCAACCCTTG-3′ R 5′-CGAGCGCTCTGCATACGACA-3′ | 65 | 278 |
OTUx | Xf2 5′-GGGTAGAGCCAGGGGTAGAG-3′ Xr2 5′-CGTAGTCCCAAGTGCCAACT-3′ | 58 | 1018 |
OTUy | Yf1 5′-CAAGAAGTACACTACCACAGCATG-3′ Yr1 5′-ACTGATTTCAGGTCTAAAACGAAT-3′ | 56 | 346 |
OTUz | Zf1 5′-TATAGTTTCTTTTGCGCGTTGC-3′ Zr1 5′-CATATCTCTTTCATGAACGAAAGG-3′ | 58 | 147 |
Species | Frequency (n) | Prevalence (%) | 95% CI | p-Value |
---|---|---|---|---|
Eimeria spp. | 36 | 24.8 | 18.5–32.5 | 0.002 |
A. galli/H. gallinarum | 37 | 25.5 | 19.1–33.2 | |
Capillaria spp. | 34 | 23.5 | 17.3–31.0 | |
Strongyle egg | 12 | 8.3 | 4.8–13.9 | |
Single infection | 43 | 29.7 | 22.8–37.5 | 0.251 |
Mixed infection | 33 | 22.8 | 16.7–30.2 | |
Total | 77 | 53.1 | 45.0–61.0 |
Species | 12–23 Months (n = 69) | 24–36 Months (n = 76) | p-Value |
---|---|---|---|
Eimeria spp. | 14 (9.7; 5.8–15.6) | 22 (15.2; 10.2–21.9) | 0.182 |
A. galli/H. gallinarum | 18 (12.4; 8.0–18.8) | 19 (13.1; 8.6–19.6) | 0.869 |
Capillaria spp. | 17 (11.7; 7.5–18.0) | 17 (11.7; 7.5–18.0) | 1 |
Strongyle eggs | 5 (3.5; 1.5–7.8) | 7 (4.8; 2.4–9.6) | 0.563 |
Single infection | 16 (11.0; 6.9–17.2) | 28 (19.3; 13.7–26.5) | 0.07 |
Mixed infection | 16 (11.0; 6.9–17.2) | 17 (11.7; 7.5–18.0) | 0.861 |
Total | 32 (22.1; 16.1–29.5) | 45 (31.0; 24.1–39.1) | 0.138 |
1–10 Chickens (n = 40) | 11–20 Chickens (n = 58) | 21–30 Chickens (n = 30) | 31–45 Chickens (n = 17) | p-Value | |
---|---|---|---|---|---|
Eimeria spp. | 6 (4.1; 1.9–8.7) | 10 (6.9; 3.8–12.2) | 10 (6.9; 3.8–12.2) | 10 (6.9; 3.8–12.2) | 0.957 |
A. galli/H. gallinarum | 6 (4.1; 1.9–8.7) | 19 (13.1; 8.6–19.6) | 5 (3.5; 1.5–7.8) | 7 (4.8; 2.4–9.6) | 0.003 |
Capillaria spp. | 7 (4.8; 2.4–9.6) | 12 (8.3; 4.8–13.9) | 10 (6.9; 3.8–12.2) | 5 (3.5; 1.5–7.8) | 0.332 |
Strongyle egg | 2 (1.4; 0.4–4.9) | 5 (3.5; 1.5–7.8) | 4 (2.8; 1.1–6.9) | 1 (0.7; 0.1–3.8) | 0.343 |
Single infection | 13 (9.0; 5.3–14.7) | 13 (9.0; 5.3–14.7) | 9 (6.2; 3.3–11.4) | 9 (6.2; 3.3–11.4) | 0.692 |
Mixed infection | 3 (2.1; 0.7–5.9) | 16 (11.0; 6.9–17.2) | 8 (5.5; 2.8–10.5) | 6 (4.1; 1.9–8.7) | 0.010 |
Total | 16 (11.0; 6.9–17.2) | 29 (20.0; 14.3–27.3) | 17 (11.7; 7.5) | 15 (10.3; 6.4–16.4) | 0.08 |
Species | Winter (n = 66) | Spring (n = 79) | p-Value |
---|---|---|---|
Eimeria spp. | 16 (11.0; 6.9–17.2) | 20 (13.8; 9.1–20.4) | 0.504 |
A. galli/H. gallinarum | 13 (9.0; 5.3–14.7) | 24 (16.6; 11.4–23.5) | 0.07 |
Capillaria spp. | 9 (6.2; 3.3–11.4) | 25 (17.2; 12.0–24.2) | 0.006 |
Strongyle egg | 4 (2.8; 1.1–6.9) | 8 (5.5; 2.8–10.5) | 0.248 |
Single infection | 17 (11.7; 7.5–18.0) | 26 (17.9; 12.5–25.0) | 0.169 |
Mixed infection | 11 (7.6; 4.3–13.1) | 22 (15.2; 10.2–21.9) | 0.05 |
Total | 29 (20.0; 14.3–27.3) | 48 (33.1; 26.0–41.1.) | 0.03 |
Frequency (n) | Prevalence (%) | 95% CI | p-Value | |
---|---|---|---|---|
Single infection | ||||
Eimeria spp. | 16 | 11.0 | 6.9–17.2 | 0.121 |
Ascaridia/Heterakis | 11 | 7.6 | 4.3–13.1 | |
Capillaria spp. | 10 | 6.9 | 3.8–12.2 | |
Strongyle | 5 | 3.5 | 1.5–7.8 | |
Mixed infection | ||||
E + A/H | 8 | 5.5 | 2.8–10.5 | 0.03 |
E + C | 3 | 2.1 | 0.7–5.9 | |
A/H + C | 10 | 6.9 | 3.8–12.2 | |
A/H + S | 2 | 1.4 | 0.4–4.9 | |
E + A/H + C | 5 | 3.5 | 1.5–7.8 | |
E + C + S | 4 | 2.8 | 1.1–6.9 | |
A/H + C + S | 1 | 0.7 | 0.1–3.8 |
Species | Frequency (n) | Prevalence (%) | 95% CI | p-Value |
---|---|---|---|---|
E. acervulina | 15 | 41.7 | 27.1–57.8 | 0.002 |
E. tenella | 10 | 27.8 | 15.9–44.0 | |
E. praecox | 6 | 16.7 | 7.9–31.9 | |
E. brunetti | 6 | 16.7 | 7.9–31.9 | |
OTUy | 3 | 8.3 | 2.9–21.8 | |
OTUz | 3 | 8.3 | 2.9–21.8 | |
E. mitis | 2 | 5.6 | 1.5–18.1 | |
A + P | 1 | 2.8 | 0.5–14.2 | 0.699 |
A + T | 3 | 8.3 | 2.9–21.8 | |
A + B | 3 | 8.3 | 2.9–21.8 | |
M + P | 1 | 2.8 | 0.5–14.2 | |
M + T | 1 | 2.8 | 0.5–14.2 | |
P + T | 3 | 8.3 | 2.9–21.8 | |
A + P + T | 2 | 5.6 | 1.5–18.1 | 0.818 |
A + M + P + T | 1 | 2.8 | 0.5–14.2 | |
A + P + T + B | 2 | 5.6 | 1.5–18.1 |
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Coroian, M.; Fábián-Ravasz, T.-Z.; Dobrin, P.R.; Györke, A. Occurrence of Eimeria spp. and Intestinal Helminths in Free-Range Chickens from Northwest and Central Romania. Animals 2024, 14, 563. https://doi.org/10.3390/ani14040563
Coroian M, Fábián-Ravasz T-Z, Dobrin PR, Györke A. Occurrence of Eimeria spp. and Intestinal Helminths in Free-Range Chickens from Northwest and Central Romania. Animals. 2024; 14(4):563. https://doi.org/10.3390/ani14040563
Chicago/Turabian StyleCoroian, Mircea, Tünde-Zsuzsánna Fábián-Ravasz, Patricia Roxana Dobrin, and Adriana Györke. 2024. "Occurrence of Eimeria spp. and Intestinal Helminths in Free-Range Chickens from Northwest and Central Romania" Animals 14, no. 4: 563. https://doi.org/10.3390/ani14040563