L-phenylalanine Increased Gut Hormone Secretion through Calcium-Sensing Receptor in the Porcine Duodenum
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sampling and Perfusion of the Pig Duodenum
2.2. Total RNA Extraction, Protocol of cDNA Formation, and RT-qPCR
2.3. Determination of Gut Hormone Concentration
2.4. Western Blotting Analysis
2.5. Statistical Analysis
3. Results
3.1. CaSR mRNA Expression and Gut Hormone Secretion after Treatment with Different Phe Concentrations
3.2. Phe-Regulated Expression and Secretion of Gut Hormones Was Mediated Via a CaSR-Dependent Mechanism
3.3. [Ca2+]e Modulated Phe-Induced CaSR and Gut Hormone Gene Expression and Gut Hormone Secretion
3.4. CaSR and Its Downstream Signaling Proteins Are Involved in Gut Hormone Secretion
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Feng, J.; Kang, C.; Wang, C.; Ding, L.; Zhu, W.; Hang, S. L-phenylalanine Increased Gut Hormone Secretion through Calcium-Sensing Receptor in the Porcine Duodenum. Animals 2019, 9, 476. https://doi.org/10.3390/ani9080476
Feng J, Kang C, Wang C, Ding L, Zhu W, Hang S. L-phenylalanine Increased Gut Hormone Secretion through Calcium-Sensing Receptor in the Porcine Duodenum. Animals. 2019; 9(8):476. https://doi.org/10.3390/ani9080476
Chicago/Turabian StyleFeng, Jiangyin, Cuicui Kang, Chao Wang, Liren Ding, Weiyun Zhu, and Suqin Hang. 2019. "L-phenylalanine Increased Gut Hormone Secretion through Calcium-Sensing Receptor in the Porcine Duodenum" Animals 9, no. 8: 476. https://doi.org/10.3390/ani9080476