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11 pages, 262 KiB

Open AccessArticle

Supplementation of Non-Starch Polysaccharide Enzymes Cocktail in a Corn-Miscellaneous Meal Diet Improves Nutrient Digestibility and Reduces Carbon Dioxide Emissions in Finishing Pigs

by Yuxia Chen, Dan Shen, Lilan Zhang, Ruqing Zhong, Zhengqun Liu, Lei Liu, Liang Chen and Hongfu Zhang

Animals 2020, 10(2), 232; https://doi.org/10.3390/ani10020232 - 1 Feb 2020

Cited by 16 | Viewed by 3719

Abstract

This study was carried out to evaluate the effect of the addition of the non-starch polysaccharide enzymes cocktail (NSPEC) on growth performance, nutrient digestibility and gas emissions in a corn-miscellaneous meal-based diet for finishing pigs. The NSPEC is a combination of cellulase, xylanase, [...] Read more.

This study was carried out to evaluate the effect of the addition of the non-starch polysaccharide enzymes cocktail (NSPEC) on growth performance, nutrient digestibility and gas emissions in a corn-miscellaneous meal-based diet for finishing pigs. The NSPEC is a combination of cellulase, xylanase, β-glucanase, β-mannanase, α-galactosidase and pectinase optimized by assessing the in vitro dry matter digestibility (IVDMD) of corn-miscellaneous meal diet using an in vitro method of simulating digestion in the stomach and intestine of growing pigs. Growth performance and apparent total tract digestibility (ATTD) of nutrients and energy were measured. The gas concentration of ammonia, carbon dioxide, nitrous oxide and methane in the environmental assessment chambers were determined. The gas detecting period was divided into three frequencies of manure removal of every 1d, 2d and 3d. The addition of NSPEC into the corn-miscellaneous meal diet decreased feed conversation rate (FCR) and increased the ATTD of dry matter, crude protein, gross energy, neutral detergent fiber and acid detergent fiber of pigs (p < 0.05). The digestible energy was also improved (p < 0.05) significantly by NSPEC supplementation in the diet. Furthermore, the supplementation of the NSPEC reduced (p < 0.05) carbon dioxide concentration in the chambers. The ammonia emissions were significantly increased according to average 1d, 2d and 3d manure removal procedures (p < 0.01). These results indicated that the inclusion of optimal NSPEC in a corn-miscellaneous meal diet improved growth performance, nutrient digestibility and reduced carbon dioxide emissions on finishing pigs. The accumulated manure could increase the release of ammonia in a pig house. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

12 pages, 233 KiB

Open AccessArticle

Effects of Collection Durations on the Determination of Energy Values and Nutrient Digestibility of High-Fiber Diets in Growing Pigs by Total Fecal Collection Method

by Zhengqun Liu, Ruqing Zhong, Liang Chen, Fei Xie, Kai Li, Lei Liu and Hongfu Zhang

Animals 2020, 10(2), 228; https://doi.org/10.3390/ani10020228 - 31 Jan 2020

Cited by 9 | Viewed by 11388

Abstract

This study was conducted to evaluate the effect of collection durations on the energy values and nutrient digestibility of high-fiber diets in growing pigs with a time-based total fecal collection method. A total of 24 barrows (body weight (BW): 31.1 ± 1.5 kg) [...] Read more.

This study was conducted to evaluate the effect of collection durations on the energy values and nutrient digestibility of high-fiber diets in growing pigs with a time-based total fecal collection method. A total of 24 barrows (body weight (BW): 31.1 ± 1.5 kg) were allotted to a completely randomized design with three diets. Diets included a corn–soybean meal (CSM) basal diet and two additional diets containing 20% sugar beet pulp (SBP) or defatted rice bran (DFRB) by replacing corn, soybean meal, and soybean oil in the CSM diet, respectively. Each diet was fed to eight barrows for a 7-day adaptation period followed by a 7-day total feces and urine collection period. The 7-day collection duration was divided into three collection phases, namely, phase 1 (days 8 to 11), phase 2 (days 11 to 13), and phase 3 (days 13 to 15). Then, similar portions of feces and urine from the different collection phases were composited into three additional samples (days 8 to 11, days 8 to 13, and days 8 to 15, respectively). The results showed that the digestible energy (DE), metabolizable energy (ME), and apparent total tract digestibility (ATTD) of gross energy (GE) and nutrient in experimental diets decreased linearly as the collection durations increased from a 3-day to a 7-day collection (p < 0.05). However, there were no differences in the energy values, GE, and nutrient digestibility of diets and of high-fiber ingredients between the 5-day and 7-day collection durations. In conclusion, this study suggests that a 5-day collection duration is adequate to determine the energy values and nutrient digestibility of high-fiber diets containing SBP or DFRB in growing pigs by the time-based total fecal collection method. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

12 pages, 2499 KiB

Open AccessArticle

Oregano Feed Supplementation Affects Glycoconjugates Production in Swine Gut

by Francesca Mercati, Cecilia Dall’Aglio, Gabriele Acuti, Valerio Faeti, Federico Maria Tardella, Carolina Pirino, Elena De Felice and Paola Scocco

Animals 2020, 10(1), 149; https://doi.org/10.3390/ani10010149 - 16 Jan 2020

Cited by 11 | Viewed by 4209

Abstract

This study evaluated the effects of adding oregano aqueous extract (OAE) to the diet of pig slaughtered at finisher stage. Study was performed to identify glycoconjugates and evaluate the oxidative stress levels in the duodenum and colon intestinal tracts. Glycohistochemistry was performed by [...] Read more.

This study evaluated the effects of adding oregano aqueous extract (OAE) to the diet of pig slaughtered at finisher stage. Study was performed to identify glycoconjugates and evaluate the oxidative stress levels in the duodenum and colon intestinal tracts. Glycohistochemistry was performed by staining with Periodic acid–Schiff (PAS), Alcian blue (AB) pH 2.5, AB-PAS, AB pH 1, AB pH 0.5, low iron diamine, and high iron diamine. Serial sections were pre-treated with sialidase V before staining with AB pH 2.5 (Sial-AB) preceded or not by saponification. To study oxidative stress, an immunohistochemical analysis was applied to investigate the presence of the oxidative stress target molecule Bcl-2 Associate X protein (BAX). Findings show that oregano aqueous extract supplementation improves the production of the secretion glycoconjugates involved in direct and indirect defense, thus enhancing the protection of the pig intestinal mucosa. Moreover, the reduced BAX protein immunostaining observed in both duodenum and colon of swine of the oregano-supplemented group respect to that observed in the control group suggests an enhanced antioxidant action by oregano adding. Findings could be useful for other studies aiming to reduce antibiotic use and prevent antimicrobial resistance. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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20 pages, 1509 KiB

Open AccessArticle

Impact of Dietary Supplementation of Lactic Acid Bacteria Fermented Rapeseed with or without Macroalgae on Performance and Health of Piglets Following Omission of Medicinal Zinc from Weaner Diets

by Gizaw D. Satessa, Paulina Tamez-Hidalgo, Yan Hui, Tomasz Cieplak, Lukasz Krych, Søren Kjærulff, Grete Brunsgaard, Dennis S. Nielsen and Mette O. Nielsen

Animals 2020, 10(1), 137; https://doi.org/10.3390/ani10010137 - 15 Jan 2020

Cited by 19 | Viewed by 6895

Abstract

The feeding of medicinal zinc oxide (ZnO) to weaner piglets will be phased out after 2022 in Europe, leaving pig producers without options to manage post-weaning disorders. This study assessed whether rapeseed meal, fermented alone (FRM) or co-fermented with a single (Ascophylum [...] Read more.

The feeding of medicinal zinc oxide (ZnO) to weaner piglets will be phased out after 2022 in Europe, leaving pig producers without options to manage post-weaning disorders. This study assessed whether rapeseed meal, fermented alone (FRM) or co-fermented with a single (Ascophylum nodosum; FRMA), or two (A. nodossum and Saccharina latissima; FRMAS) brown macroalagae species, could improve weaner piglet performance and stimulate intestinal development as well as maturation of gut microbiota in the absence of in-feed zinc. Weaned piglets (n = 1240) were fed, during 28–85 days of age, a basal diet with no additives (negative control; NC), 2500 ppm in-feed ZnO (positive control; PC), FRM, FRMA or FRMAS. Piglets fed FRM and FRMA had a similar or numerically improved, respectively, production performance compared to PC piglets. Jejunal villus development was stimulated over NC in PC, FRM and FRMAS (gender-specific). FRM enhanced colon mucosal development and reduced signs of intestinal inflammation. All fermented feeds and PC induced similar changes in the composition and diversity of colon microbiota compared to NC. In conclusion, piglet performance, intestinal development and health indicators were sustained or numerically improved when in-feed zinc was replaced by FRM. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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10 pages, 244 KiB

Open AccessArticle

Effect of Phytase Derived from the E. coli AppA Gene on Weaned Piglet Performance, Apparent Total Tract Digestibility and Bone Mineralization

by Zuzanna Wiśniewska, Lode Nollet, Anouk Lanckriet, Erik Vanderbeke, Spas Petkov, Nikolay Outchkourov, Małgorzata Kasprowicz-Potocka, Anita Zaworska-Zakrzewska and Sebastian A. Kaczmarek

Animals 2020, 10(1), 121; https://doi.org/10.3390/ani10010121 - 11 Jan 2020

Cited by 6 | Viewed by 2961

Abstract

The objective of this study was to assess the effect of novel appAT1 and appAT2 phytase inclusion at 250 phytase units (FTU)/kg on weaned piglet performance, the apparent total tract digestibility of P and Ca, and bone mineralization. Piglets (48 males) were randomly [...] Read more.

The objective of this study was to assess the effect of novel appAT1 and appAT2 phytase inclusion at 250 phytase units (FTU)/kg on weaned piglet performance, the apparent total tract digestibility of P and Ca, and bone mineralization. Piglets (48 males) were randomly divided into four treatment groups: a positive control (PC), with recommended levels of phosphorus (P) and calcium (Ca), a negative control (NC) deficient in P and Ca, and two experimental groups with NC diets supplemented with phytase derived from the appA gene of Escherichia coli. Diets fed in a mashed form were divided into prestarter (0–21 days) and starter (22–42 days) periods. During the whole period of the study, the experimental diets improved (p < 0.05) the body weight gain (BWG) and feed conversion ratio (FCR) compared to the NC diet. The apparent total tract digestibility (ATTD) of the dry matter and crude protein was not significantly different among the diets. Phytase-supplemented diets improved the ATTD of P (p < 0.05) and the ATTD of Ca (p < 0.05). Bone ash content in the third metacarpal and P and Ca content were improved among the phytase supplemented diets compared to the NC diet. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

9 pages, 267 KiB

Open AccessArticle

Effect of Increasing Dietary Aminoacid Concentration in Late Gestation on Body Condition and Reproductive Performance of Hyperprolific Sows

by Senén Seoane, Pasquale De Palo, José Manuel Lorenzo, Aristide Maggiolino, Pablo González, Leticia Pérez-Ciria and Maria Angeles Latorre

Animals 2020, 10(1), 99; https://doi.org/10.3390/ani10010099 - 8 Jan 2020

Cited by 7 | Viewed by 2740

Abstract

A total of 62 highly prolific Danbred sows was used to evaluate the implications of increasing dietary amino acid (AA) concentration during late gestation (from day 77 to 107 of pregnancy) on body condition and reproductive performances. Sows were assigned to one of [...] Read more.

A total of 62 highly prolific Danbred sows was used to evaluate the implications of increasing dietary amino acid (AA) concentration during late gestation (from day 77 to 107 of pregnancy) on body condition and reproductive performances. Sows were assigned to one of the two treatments (n = 31, with similar number of sows in the second-, third- and fourth-cycle); control diet (containing 6 g of standardized ileal digestible lysine -SID Lys-)/kg) and high AA level (containing 10 g SID Lys/kg and following the ideal protein concept for the remaining essential AA). On day 108 of pregnancy, animals were moved to the farrowing-lactating facilities where they spent until weaning receiving a common standard lactation diet. After farrowing, litters were standardized to 13 piglets each. At 107 d of gestation, backfat depth was thicker in sows fed high AA concentration than in those fed control diet (p < 0.0001) but these significant differences disappeared at weaning (p > 0.05). Additionally, at farrowing, the litter size (p = 0.043) and weight (p = 0.017) were higher in sows fed high AA level. It can be concluded that the increase in the AA content in the feed during the last month of gestation could improve the body condition of the sows and their performance results. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

11 pages, 250 KiB

Open AccessArticle

Meat Quality and Fatty Acid Profiles of Chinese Ningxiang Pigs Following Supplementation with N-Carbamylglutamate

by Yueteng Xing, Xin Wu, Chunyan Xie, Dingfu Xiao and Bin Zhang

Animals 2020, 10(1), 88; https://doi.org/10.3390/ani10010088 - 6 Jan 2020

Cited by 16 | Viewed by 3122

Abstract

The present study evaluated the effects of dietary N-carbamylglutamate (NCG) on carcass traits, meat quality, and fatty acid profiles in the longissimus dorsi muscle and adipose tissues of Chinese Ningxiang pigs. A total of 36 castrated female pigs with a similar initial [...] Read more.

The present study evaluated the effects of dietary N-carbamylglutamate (NCG) on carcass traits, meat quality, and fatty acid profiles in the longissimus dorsi muscle and adipose tissues of Chinese Ningxiang pigs. A total of 36 castrated female pigs with a similar initial weight (43.21 ± 0.57 kg) were randomly assigned to two treatments (with six pens per treatment and three pigs per pen) and fed either a basal diet or a basal diet supplemented with 0.08% NCG for 56 days. Results showed that dietary NCG reduced shear force (p = 0.004) and increased drip loss (p = 0.044) in longissimus dorsi muscle of Ningxiang pigs. Moreover, increased levels of oleic acid (C18:1n9c) (p = 0.009), paullinic acid (C20:1) (p = 0.004), and α-linolenic acid (C18:3n3) (p < 0.001), while significant reduction in the proportions of arachidonic acid (C20:4n6) (p < 0.001) and polyunsaturated fatty acid (PUFA) (p = 0.017) were observed in the longissimus dorsi muscle of pigs fed NCG when compared with those fed the control diet. As for adipose tissues, the C20:1 (p = 0.045) proportion in dorsal subcutaneous adipose (DSA), as well as the stearic acid (C18:0) (p = 0.018) level in perirenal adipose (PA) were decreased when pigs were fed the NCG diet compared with those of the control diet. In contrast, the margaric acid (C17:0) (p = 0.043) proportion in PA were increased. Moreover, the NCG diet produced PA with a greater proportion of total PUFAs (p = 0.001) (particularly linoleic acid (C18:2n6c) (p = 0.001)) compared with those produced by the control diet. These findings suggest that dietary NCG has beneficial effects by decreasing the shear force and improving the healthfulness of fatty acid profiles, providing a novel strategy for enhancing meat quality of pigs. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

12 pages, 242 KiB

Open AccessArticle

Endogenous Losses of Fat and Fatty Acids in Growing Pigs Are Not Affected by Vegetable Oil Sources but by the Method of Estimation

by Lu Wang, Li Wang, Zhiqian Lyu, Bingbing Huang, Qile Hu and Changhua Lai

Animals 2020, 10(1), 48; https://doi.org/10.3390/ani10010048 - 25 Dec 2019

Cited by 14 | Viewed by 2206

Abstract

An experiment was conducted to determine the effect of oil sources with differing degrees of fatty acid saturation on endogenous losses of fat (ELF) and fatty acids (ELFA) in growing pigs, in which endogenous losses were estimated by two methods. Sixty-eight growing barrows [...] Read more.

An experiment was conducted to determine the effect of oil sources with differing degrees of fatty acid saturation on endogenous losses of fat (ELF) and fatty acids (ELFA) in growing pigs, in which endogenous losses were estimated by two methods. Sixty-eight growing barrows (initial body weight 31.13 ± 4.44 kg) were randomly allotted to a completely randomized design with 17 diets. Sixteen added-oil diets were formulated by adding four levels (2%, 4%, 6% and 8%) of palm oil (PO), soybean oil (SBO), flaxseed oil (FSO) and rapeseed oil (RSO) to a diet poor in fat, respectively. One fat-free diet was formulated from cornstarch, soy protein isolate and sucrose. All diets contained chromic dioxide (0.4%) as an indigestible marker. Results indicated that, according to the regression equations, the amounts of ELF in PO, SBO, FSO and RSO were 6.28, 5.30, 4.17 and 4.84 g/kg of dry matter intake (DMI), respectively. The true total tract digestibility of fat was greater (p < 0.05) for FSO and RSO than for PO, and the ELFA were different from 0 only for C16:0, C18:0 and C18:1 in FSO, and C16:0 and C18:0 in RSO (p < 0.05). The estimated values for ELF and ELFAs in pigs fed PO, SBO, FSO or RSO were not different. The amount of ELF determined by the fat-free diet method was 2.60 g/kg DMI, and the amounts of C16:0, C18:0, C18:1 and C18:2 in ELFAs were 0.28, 0.26, 0.03 and 0.02 g/kg DMI, respectively. The fat-free diet method had lower ELF and ELFA values compared with the regression method (p < 0.01). Collectively, dietary vegetable oil sources do not affect estimation of ELF and ELFA, but different evaluation methods lead to varying estimates of endogenous losses in pigs. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

14 pages, 2301 KiB

Open AccessArticle

Effects of Activated Charcoal-Herb Extractum Complex on Antioxidant Status, Lipid Metabolites and Safety of Excess Supplementation in Weaned Piglets

by Liqi Wang, Lin Zhu, Limin Gong, Xin Zhang, Yubo Wang, Jianling Liao, Linfu Ke and Bing Dong

Animals 2019, 9(12), 1151; https://doi.org/10.3390/ani9121151 - 15 Dec 2019

Cited by 5 | Viewed by 3167

Abstract

This study was aimed at evaluating the effects of activated charcoal-herb extractum complex (CHC) on antioxidant status, serum lipid metabolites and its safety supplement in weaning piglets. In experiment 1, a total of 216 piglets (Duroc × Landrace × Large White) weaned at [...] Read more.

This study was aimed at evaluating the effects of activated charcoal-herb extractum complex (CHC) on antioxidant status, serum lipid metabolites and its safety supplement in weaning piglets. In experiment 1, a total of 216 piglets (Duroc × Landrace × Large White) weaned at 28 days of age with initial body weight of 8.55 ± 1.18 kg were assigned randomly to six treatment groups. each treatment group had six pens, with six pigs per pen. Pigs were fed a corn-soybean meal-based diet supplemented with 500, 1000, 1500 or 2000 mg kg⁻¹ of CHC over two 14-d periods. Diets supplemented with 0 and 1000 mg kg⁻¹ of montmorillonite (MMT) were set as the negative and positive controls, respectively. In experiment 2, pigs (n = 108) weaned at 28 days of age with initial body weight of 8.58 ± 0.04 kg were randomly assigned to three treatment groups. Each treatment group had six pens, with six pigs per pen. Pigs were fed a corn-soybean meal-based diet supplemented with 0, 1000 or 10,000 mg kg⁻¹ of CHC over two 14-d periods. In experiment 1, on day 14, supplementation with CHC significantly decreased very low-density lipoprotein (VLDL) concentration while they decreased low-density lipoprotein (LDL) concentration on d 28, CHC at 500, 1000 or 1500 mg kg⁻¹ significantly increase high-density lipoprotein (HDL) concentration. Supplementation with 500 or 1000 mg kg⁻¹ CHC reduced serum malondialdehyde (MDA) concentration during the entire experimental period and increased the concentration of serum total superoxide dismutase (T-SOD) on d 14. CHC at 500 or 1000 mg kg⁻¹ significantly reduced the liver MDA concentration and increased liver T-SOD concentration. In experiment 2, increased ADG was obvious during the first 14 days and the whole period in 1000 mg kg⁻¹ supplemented pigs, similarly F: G was lowest in the first 14 days. There was no difference in growth performance, visceral index, haematological and serum biochemical parameters and visceral organs morphology between pigs fed 10,000 mg kg⁻¹ of CHC and control. Together, 500 to 1000 mg kg⁻¹ CHC was confirmed to improve antioxidant status, and serum lipid metabolites in this study and excess supplementation of CHC is safe in weaning piglets. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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10 pages, 260 KiB

Open AccessArticle

Effects of Different Protein Levels on the Nitrogen Balance, Performance and Slaughtering Traits of Cinta Senese Growing Pigs

by Chiara Aquilani, Francesco Sirtori, Oreste Franci, Anna Acciaioli, Riccardo Bozzi, Doria Benvenuti, Marjeta Čandek-Potokar and Carolina Pugliese

Animals 2019, 9(12), 1021; https://doi.org/10.3390/ani9121021 - 22 Nov 2019

Cited by 6 | Viewed by 2762

Abstract

Cinta Senese is characterized by slow growth rates, which implies different nutritional requirements compared to major pig breeds. Four different crude protein levels (120, 140, 160 and 180 g/kg on as-fed basis, denoted as CP12, CP14, CP16 and CP18) were tested to assess [...] Read more.

Cinta Senese is characterized by slow growth rates, which implies different nutritional requirements compared to major pig breeds. Four different crude protein levels (120, 140, 160 and 180 g/kg on as-fed basis, denoted as CP12, CP14, CP16 and CP18) were tested to assess the optimal protein requirements of Cinta Senese pigs during the growing phase. The in vivo performance, slaughtering traits and nitrogen balance were evaluated using individual pens and metabolic crates. Increasing the protein level in feed lowered the average daily gain (from 0.76 to 0.71 kg/d), final weight (63.0 kg for CP12 versus 60.7 kg for CP16) and reduced the protein conversion efficiency (from 0.37 to 0.58). Also, protein conversion in lean protein linearly increased from CP12 (4.82) to CP18 (7.43), which implies a worsening in the protein utilization efficiency. The nitrogen balance showed higher loss of N through urine (from 0.68 g/d/kg metabolic weight for CP12 to 1.14 g/d/kg metabolic weight for CP18) as the dietary CP levels increased, and a decrease in the biological value (51.78 for CP12 versus 36.54 for CP16). The results indicated that the CP12 diet was adequate for fulfilling the Cinta Senese protein requirements during the growing phase. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

12 pages, 631 KiB

Open AccessArticle

Effects of Dietary Supplementation of Alpha-Ketoglutarate in a Low-Protein Diet on Fatty Acid Composition and Lipid Metabolism Related Gene Expression in Muscles of Growing Pigs

by Jiashun Chen, Haihan Zhang, Hu Gao, Baoju Kang, Fengming Chen, Yinghui Li, Chenxing Fu and Kang Yao

Animals 2019, 9(10), 838; https://doi.org/10.3390/ani9100838 - 21 Oct 2019

Cited by 12 | Viewed by 4273

Abstract

The aim of the current study was to investigate whether dietary supplementation with alpha-ketoglutarate (AKG) in a reduced crude protein (CP) diet would affect fatty acid composition and lipid metabolism related gene expression in the muscles of growing pigs. A total of 27 [...] Read more.

The aim of the current study was to investigate whether dietary supplementation with alpha-ketoglutarate (AKG) in a reduced crude protein (CP) diet would affect fatty acid composition and lipid metabolism related gene expression in the muscles of growing pigs. A total of 27 Large White × Landrace growing pigs at 44 ± 1 d of age (11.96 ± 0.18 kg) were randomly allocated to three treatments (n = 9). Dietary treatments included: (1) normal protein diet with 20% crude protein (CP) (NP); (2) a low crude protein diet formulated to contain approximately 17% CP (LP); and (3) a low crude protein diet with 17% CP supplemented with 1% AKG at the expense of regular corn components (ALP). The experimental trial lasted 35 d. The results showed that compared with the NP and LP diets, supplementation with AKG in a low-protein diet increased the intramuscular fat (IMF), oleic acid (C18:1n-9), and monounsaturated fatty acid (MUFA) contents (p < 0.05), and tended to increase the percentage of palmitoleic acid (C16:1) and stearic acid (C18:0) (p < 0.10) in the biceps femoris and longissimus dorsi muscles of growing pigs. These effects may be associated with increased relative mRNA expression levels of fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), adipocyte determination and differentiation factor 1 (ADD1), fatty acid binding protein 4 (FABP4), and stearoyl-CoA desaturase (SCD) in skeletal muscle, indicating that AKG might be involved in the differential regulation of some key lipogenic genes in skeletal muscles of pigs. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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8 pages, 411 KiB

Open AccessArticle

Effects of the Feed: Water Mixing Proportion on Diet Digestibility of Growing Pigs

by Cinta Sol, Lorena Castillejos, Sergi López-Vergé, Ramon Muns and Josep Gasa

Animals 2019, 9(10), 791; https://doi.org/10.3390/ani9100791 - 12 Oct 2019

Cited by 2 | Viewed by 3688

Abstract

The effect of different water-to-feed ratios on apparent total tract digestibility of energy and nutrients were evaluated in growing–finishing pigs. In trial 1 (26 d), 16 female pigs (46.7 ± 1.98 kg of body weight, BW) were individually assigned to four treatments (n [...] Read more.

The effect of different water-to-feed ratios on apparent total tract digestibility of energy and nutrients were evaluated in growing–finishing pigs. In trial 1 (26 d), 16 female pigs (46.7 ± 1.98 kg of body weight, BW) were individually assigned to four treatments (n = 4). In trial 1, pigs were fed a control diet in dry form (CON) and in blend form with water-to-feed ratios of 0.6:1, 2.1:1 and 2.7:1. In trial 2 (26 d), rearranged animals (65.4 ± 3.14 kg of BW) were assigned a control diet in dry form (CON) and ratios of 1.35:1, 2.7:1 and 3.5:1. In trial 1, pigs fed on ratios of 2.1:1 and 2.7:1 had a higher organic matter digestibility (OMd) and gross energy digestibility (GEd) than CON. In trial 2, pigs fed on ratios of 1.35:1, 2.7:1, and 3.5:1 had a higher OMd and GEd than CON. Quadratic regressions showed the maximum dilution rate to reach higher digestibility coefficients at 1.83:1 and at 2.7:1 for trials 1 and 2, respectively. During trial 1, pigs on the 0.6:1 dilution rate had higher weight gain than those on CON. The water-to-feed ratio that optimized OM and GE digestibility may increase with the age of the pigs. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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12 pages, 657 KiB

Open AccessArticle

Effects of Forsythia Suspense Extract as an Antibiotics Substitute on Growth Performance, Nutrient Digestibility, Serum Antioxidant Capacity, Fecal Escherichia coli Concentration and Intestinal Morphology of Weaned Piglets

by Shenfei Long, Li Liu, Sujie Liu, Shad Mahfuz and Xiangshu Piao

Animals 2019, 9(10), 729; https://doi.org/10.3390/ani9100729 - 26 Sep 2019

Cited by 20 | Viewed by 3378

Abstract

The aim of this study is to determine the efficiency of Forsythia suspense extract (FSE) as an antibiotics substitute on performance, nutrient digestibility, serum antioxidant capacity, fecal Escherichia coli concentration and intestinal morphology of weaned piglets. A total of 108 Duroc × (Landrace [...] Read more.

The aim of this study is to determine the efficiency of Forsythia suspense extract (FSE) as an antibiotics substitute on performance, nutrient digestibility, serum antioxidant capacity, fecal Escherichia coli concentration and intestinal morphology of weaned piglets. A total of 108 Duroc × (Landrace × Yorkshire) weaned piglets (28 days (d) weaned, average body weight of 8.68 ± 1.36 kg) were randomly assigned into three dietary treatments, six pens per treatment, three barrows and three gilts per pen. The treatments contained a corn-soybean meal basal diet (CTR), an antibiotic diet (basal diet + 75 mg/kg chlortetracycline; CTC), and an FSE diet (basal diet + 200 mg/kg FSE; FSE). The experiment included phase 1 (d 1 to 14), phase 2 (d 15 to 28) and phase 3 (d 29 to 35). Compared with CTR, piglets fed FSE show improved (p < 0.05) average daily gain (ADG) and average daily feed intake in phase 2, as well as enhanced (p < 0.05) ADG from day 15 to 35 and day 1 to 28. Piglets supplemented with CTC and FSE showed a reduced (p < 0.05) diarrhea rate in phase 1, while piglets fed FSE showed enhanced (p < 0.05) apparent total tract digestibility (ATTD) of dry matter, organic matter, crude protein and gross energy, as well as lower (p < 0.05) nitrogen output in phase 2 compared with CTR and CTC. The content in the form of Colony-Forming Units (CFUs) of fecal E. coli on day 14 and 28 was lower (p < 0.05) in piglets fed FSE in comparison with CTR. The contents of total antioxidant capacity, superoxide dismutase and catalase in serum are enhanced (p < 0.05) compared with CTR and CTC, whereas the concentration of malondialdehyde in serum was decreased (p < 0.05) for piglets fed FSE on day 28 compared with CTC. The villus height to crypt depth ratio in ileum was numerically higher (p < 0.05) in piglets fed FSE in comparison with CTR. In conclusion, dietary FSE supplementation could substitute CTC in improving antioxidant capacity, nutrients digestibility and reducing fecal E. coli content, so as to reduce nitrogen output and diarrhea rate, and eventually improve performance in weaned piglets. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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12 pages, 413 KiB

Open AccessArticle

Responses of Pigs of Different Genotypes to a Variation in the Dietary Indispensable Amino Acid Content in Terms of Their Growth, and Carcass and Meat Quality Traits

by Stefano Schiavon, Mirco Dalla Bona, Giuseppe Carcò, Enrico Sturaro and Luigi Gallo

Animals 2019, 9(8), 508; https://doi.org/10.3390/ani9080508 - 31 Jul 2019

Cited by 6 | Viewed by 3291

Abstract

We studied the response of pigs from two crossbred genetic lines (GL) in the 60 to 145 kg body weight interval to a variation in the indispensable amino acid (AA) content of their feed. Ninety-six barrows of two paternal GLs (Hypor Maxter and [...] Read more.

We studied the response of pigs from two crossbred genetic lines (GL) in the 60 to 145 kg body weight interval to a variation in the indispensable amino acid (AA) content of their feed. Ninety-six barrows of two paternal GLs (Hypor Maxter and PIC 337) were housed in eight pens and fed quasi ad libitum on feeds differing in their standardized ileal digestible (SID) indispensable AA contents. Pigs in four pens received feeds containing 9.4 to 8.0 g/kg of SID Lys (HAA), considered non-limiting, while the others received feeds containing 8.5 to 6.5 g/kg of SID Lys (LAA). The two feeds had identical indispensable lysine, methionine, tryptophan and threonine contents per unit of crude protein (CP). Feed intake, growth, carcass weight, and the weights of the lean and fat cuts were recorded, and samples of longissimus lumborum were analyzed. Data were analyzed using a two-way factorial mixed model. The LAA feed lowered the estimated N excretion (p < 0.001) compared with HAA, without affecting growth, carcass or meat quality traits. Genetic line had trivial effects and the AA level × genotype interaction was never significant. The two pig genotypes did not differ sufficiently in growth potential and leanness to respond differently. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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9 pages, 548 KiB

Open AccessArticle

Dietary Inclusion of 1,3-Butanediol Increases Dam Circulating Ketones and Increases Progeny Birth Weight

by Udani Wijesiriwardana, John R. Pluske, Jessica R. Craig, Jeremy Cottrell and Frank R. Dunshea

Animals 2019, 9(8), 479; https://doi.org/10.3390/ani9080479 - 24 Jul 2019

Cited by 4 | Viewed by 2732

Abstract

1,3-Butanediol (BD) is a ketogenic substance that can improve piglet growth and survival and potentially increase performance in gilt progeny when provided as a dietary supplement during late gestation. Gilts (n = 77; parity 1) and sows (n = 74; parities 2 and [...] Read more.

1,3-Butanediol (BD) is a ketogenic substance that can improve piglet growth and survival and potentially increase performance in gilt progeny when provided as a dietary supplement during late gestation. Gilts (n = 77; parity 1) and sows (n = 74; parities 2 and 3) were fed either a standard commercial gestation diet or a diet supplemented with 4% BD from day 90 of gestation until farrowing. Dams fed with diets supplemented with BD had higher plasma beta-hydroxybutyrate (p = 0.01) and lower non-esterified fatty acid concentrations (p < 0.001). The percentage of progeny that were light-for-age (<1.1 kg) at birth was decreased by BD (18.2 vs. 13.5%, p < 0.006), particularly in gilts (24.0 vs. 18.3%, p < 0.034). Individual birth weights and litter weights birth weights tended to be increased by the BD diet (p = 0.085 and 0.078; respectively) although these effects were not maintained to weaning. Pre-weaning mortality was greater in gilt than in sow progeny and was not altered by dietary BD. Feeding BD in late gestation can improve birth weight, but further work is needed to see if these effects are carried through subsequent stages of growth, particularly in gilt progeny. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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13 pages, 2081 KiB

Open AccessArticle

L-phenylalanine Increased Gut Hormone Secretion through Calcium-Sensing Receptor in the Porcine Duodenum

by Jiangyin Feng, Cuicui Kang, Chao Wang, Liren Ding, Weiyun Zhu and Suqin Hang

Animals 2019, 9(8), 476; https://doi.org/10.3390/ani9080476 - 24 Jul 2019

Cited by 22 | Viewed by 3476

Abstract

Luminal amino acids have a pivotal role in gut hormone secretion, and thereby modulate food intake and energy metabolism. However, the mechanisms by which amino acids exert this effect remains unknown. The purpose of this research was to investigate the response of L-phenylalanine [...] Read more.

Luminal amino acids have a pivotal role in gut hormone secretion, and thereby modulate food intake and energy metabolism. However, the mechanisms by which amino acids exert this effect remains unknown. The purpose of this research was to investigate the response of L-phenylalanine (L-Phe) to gut hormone secretion and its underlying mechanisms by perfusing the pig duodenum. Eighty mM L-Phe and extracellular Ca²⁺ stimulated cholecystokinin (CCK) and glucose-dependent insulinotropic peptide (GIP) release, and upregulated the mRNA expression of the calcium-sensing receptor (CaSR), CCK, and GIP. Western blotting results showed that L-Phe also elevated the protein levels of CaSR, the inositol 1,4,5-triphosphate receptor (IP₃R), and protein kinase C (PKC). However, the CaSR inhibitor NPS 2143 reduced the mRNA expression of CaSR, CCK, and GIP, and the secretion of CCK and GIP, as well as the protein level of CaSR, IP₃R, and PKC. These results indicated that Phe stimulated gut secretion through a CaSR-mediated pathway and its downstream signaling molecules, PKC and IP₃R. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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17 pages, 346 KiB

Open AccessArticle

Effects of the Ratio of Insoluble Fiber to Soluble Fiber in Gestation Diets on Sow Performance and Offspring Intestinal Development

by Yang Li, Lijia Zhang, Haoyu Liu, Yi Yang, Jiaqi He, Meng Cao, Min Yang, Wei Zhong, Yan Lin, Yong Zhuo, Zhengfeng Fang, Lianqiang Che, Bin Feng, Shengyu Xu, Jian Li, Xilun Zhao, Xuemei Jiang and De Wu

Animals 2019, 9(7), 422; https://doi.org/10.3390/ani9070422 - 5 Jul 2019

Cited by 35 | Viewed by 5292

Abstract

To investigate the effects of the ratio of insoluble fiber to soluble fiber (ISF:SF) on sow performance and piglet intestinal development, we randomly assigned 64 gilts to four treatments comprising diets with the same level of dietary fiber, but different ISF:SF values of [...] Read more.

To investigate the effects of the ratio of insoluble fiber to soluble fiber (ISF:SF) on sow performance and piglet intestinal development, we randomly assigned 64 gilts to four treatments comprising diets with the same level of dietary fiber, but different ISF:SF values of 3.89 (T1), 5.59 (T2), 9.12 (T3), and 12.81 (T4). At birth and weaning, six piglets per treatment at each phase were slaughtered for sampling. As ISF:SF increased, the mean piglet body weight (BW) at weaning and piglet BW gain, which were all significantly higher in T1 and T2 compared with T3 and T4 (p < 0.05), showed a linear decrease (p < 0.05); the crypt depth of the jejunum in weaned piglets linearly increased, whereas the duodenal weight, jejunal villus height, and villus height/crypt depth in newborn piglets and enzymatic activity of lactase, sucrase, and maltase linearly decreased (p < 0.05). No differences were observed in the yield and composition of milk (p > 0.05). Moreover, when the ISF:SF was 3.89 in gestation diets, higher piglet BW at weaning occurred, possibly because the ISF:SF affected development and enzymatic activity in the small intestine—effects related to digestion and absorption of nutrients—and consequently enhanced piglet BW gain. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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14 pages, 269 KiB

Open AccessArticle

Dietary Inclusion of Monosodium Glutamate in Gestating and Lactating Sows Modifies the Preference Thresholds and Sensory-Motivated Intake for Umami and Sweet Solutions in Post-Weaned Pigs

by Sergio A. Guzmán-Pino, Cristian Lazcano, Valeria De Luca, Jaime Figueroa, Carolina Valenzuela and Eugeni Roura

Animals 2019, 9(6), 336; https://doi.org/10.3390/ani9060336 - 10 Jun 2019

Cited by 10 | Viewed by 4448

Abstract

Pigs show an innate preference for umami (monosodium glutamate, MSG) taste. Nevertheless, the influence of a pre and postnatal umami exposure remains unclear. An experiment was conducted to test the hypothesis that MSG inclusion into maternal diets would modify the feeding behavior of [...] Read more.

Pigs show an innate preference for umami (monosodium glutamate, MSG) taste. Nevertheless, the influence of a pre and postnatal umami exposure remains unclear. An experiment was conducted to test the hypothesis that MSG inclusion into maternal diets would modify the feeding behavior of post-weaning pigs. A total of 22 sows were selected on day 85 of gestation and randomly assigned to one of two gestating and lactating programs (standard commercial diets without or with 50 g/kg of MSG). Later, 208 pigs born from these sows were selected to evaluate their preference thresholds, sensory-motivated intake, total consumption, and consumption patterns for MSG and sucrose solutions. Pigs born from MSG-fed sows showed lower (p < 0.045) preference thresholds for MSG and sucrose than did animals born from control sows, and displayed an increased (p < 0.050) sensory-motivated intake for sucrose and decreased for MSG. Conversely, no differences (p > 0.05) were observed in the total consumption or consumption patterns of MSG or sucrose solutions among pigs born from control and MSG-fed sows. It is concluded that the feeding behavior of nursery pigs can be influenced by pre and postnatal inclusion of a taste active compound into maternal diets. It would appear that a compensatory mechanism to balance dietary nutrients might be in place. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

16 pages, 724 KiB

Open AccessArticle

Immune System Stimulation Reduces the Efficiency of Whole-Body Protein Deposition and Alters Muscle Fiber Characteristics in Growing Pigs

by Whitney D. McGilvray, Bradley Johnson, Hailey Wooten, Amanda R. Rakhshandeh and Anoosh Rakhshandeh

Animals 2019, 9(6), 323; https://doi.org/10.3390/ani9060323 - 6 Jun 2019

Cited by 7 | Viewed by 3443

Abstract

The effects of immune system stimulation (ISS), induced by repeated injection of Escherichia coli lipopolysaccharide, on the whole-body protein synthesis versus degradation rates, the efficiency of protein deposition (PD), and muscle fiber characteristics in pigs were evaluated. Twelve growing gilts were assigned to [...] Read more.

The effects of immune system stimulation (ISS), induced by repeated injection of Escherichia coli lipopolysaccharide, on the whole-body protein synthesis versus degradation rates, the efficiency of protein deposition (PD), and muscle fiber characteristics in pigs were evaluated. Twelve growing gilts were assigned to two levels of amino acid intake that was predicted based on the potential of each group’s health status for PD and feed intake. Isotope tracer, nitrogen balance, and immunohistochemical staining techniques were used to determine protein turnover, PD, and muscle fiber characteristics, respectively. Protein synthesis, degradation, and PD were lower in immune-challenged pigs than in control pigs (p < 0.05). Strong tendencies for a higher protein synthesis-to-PD ratio (p = 0.055) and a lower protein synthesis-to-degradation ratio (p = 0.065) were observed in immune-challenged pigs. A decrease in muscle cross-sectional area of fibers and a shift from myosin heavy chain (MHC)-II towards MHC-I fibers (p < 0.05) were observed in immune-challenged pigs. These results indicated that ISS reduces PD not only by suppressing the whole-body protein synthesis and degradation rates, but also by decreasing the efficiency of PD in growing pigs. In addition, ISS induces atrophy in skeletal muscles and favors a slow-twitch oxidative fiber type composition. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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11 pages, 280 KiB

Open AccessArticle

Effects of Protein Restriction on Performances and Meat Quality of Cinta Senese Pig Reared in an Organic System

by Chiara Aquilani, Francesco Sirtori, Oreste Franci, Anna Acciaioli, Riccardo Bozzi, Antonio Pezzati and Carolina Pugliese

Animals 2019, 9(6), 310; https://doi.org/10.3390/ani9060310 - 31 May 2019

Cited by 6 | Viewed by 3348

Abstract

In lean genotypes, protein restriction during growing increases intramuscular fat content without affecting the overall carcass fatness. The present study aims to assess the feasibility of applying this feeding management on an obese pig, the Cinta Senese, since obese genotypes are characterized by [...] Read more.

In lean genotypes, protein restriction during growing increases intramuscular fat content without affecting the overall carcass fatness. The present study aims to assess the feasibility of applying this feeding management on an obese pig, the Cinta Senese, since obese genotypes are characterized by great lipogenic potential often leading to excessively high backfat deposits. Twenty pigs of average weight 38 kg, were divided in two groups, the first group was fed a protein restricted diet (9% of crude protein), while the second one a normal diet (13.5% of crude protein). During finishing, both groups were fed the same diet (10% of crude protein). Average daily gain, protein conversion index, backfat thickness, carcass weight, and prime cuts were determined. A loin sample joint was dissected in intermuscular fat, bone, subcutaneous fat, longissimus lumborum, and psoas major. On longissimus lumborum, physical and chemical analysis was carried out. The fatty acid profile of longissimus lumborum and loin subcutaneous fat were determined. Data were analyzed by analysis of variance. Protein conversion index resulted lower in the restricted group, while backfat was slightly greater. Meat quality traits were not affected by feeding management. Slightly modifications in subcutaneous outer layer fatty acids profile were observed. The protein restriction during growing did not seem a suitable mode of feeding management for Cinta Senese pigs. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

11 pages, 275 KiB

Open AccessArticle

The Effects of Partially or Completely Substituted Dietary Zinc Sulfate by Lower Levels of Zinc Methionine on Growth Performance, Apparent Total Tract Digestibility, Immune Function, and Visceral Indices in Weaned Piglets

by Yuhuai Xie, Qing Zhang, Lixue Wang, Yuxi Wang, Zhenfeng Cheng, Zaibin Yang and Weiren Yang

Animals 2019, 9(5), 236; https://doi.org/10.3390/ani9050236 - 13 May 2019

Cited by 16 | Viewed by 4043

Abstract

The study aimed to evaluate the effects of replacing zinc sulfate (ZnSO₄) with a lower level of zinc methionine (ZnMet) on the growth performance, apparent total tract digestibility (ATTD) of nutrients, serum metabolites and immune functions of weaned piglets. Thirty-five weaned [...] Read more.

The study aimed to evaluate the effects of replacing zinc sulfate (ZnSO₄) with a lower level of zinc methionine (ZnMet) on the growth performance, apparent total tract digestibility (ATTD) of nutrients, serum metabolites and immune functions of weaned piglets. Thirty-five weaned Duroc × Landrace × Large White male piglets (10.69 ± 0.26 kg) were randomly allotted to five diets. The control diet was supplemented with 100 mg/kg of Zn from ZnSO₄, and experimental diets included 75 + 12.5, 50 + 25, 25 + 37.5, and 0 + 50 mg/kg of Zn from ZnSO₄ and ZnMet, respectively. The results showed that no differences were observed in growth performance, ATTD of nutrients and serum metabolites among treatments, while serum white blood cell count, lymphocyte count, IgM contents and spleen index were higher (p < 0.01) in piglets fed with 50 + 25 mg/kg of Zn. Zinc digestibility (p < 0.05), IgA content (p < 0.001) and thymus index (p < 0.05) were increased when at least 50% of ZnSO₄ was replaced by ZnMet. All the results indicated that using a lower level of ZnMet in weaned piglet’s diet instead of ZnSO₄ had no adverse impacts on ATTD of nutrients and serum metabolites; and a 50 + 25 mg/kg of Zn (from ZnSO₄ and ZnMet, respectively) diet showed the best advantages for parameters relating to immune functions. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

13 pages, 411 KiB

Open AccessArticle

Evaluation of Performance, Nitrogen Metabolism and Tissue Composition in Barrows Fed an n-3 PUFA-Rich Diet

by Mihaela Hăbeanu, Nicoleta Aurelia Lefter, Anca Gheorghe, Arabela Untea, Mariana Ropotă, Daniela-Mihaela Grigore, Iulia Varzaru and Smaranda Mariana Toma

Animals 2019, 9(5), 234; https://doi.org/10.3390/ani9050234 - 13 May 2019

Cited by 10 | Viewed by 3358

Abstract

We investigated changes in nitrogen metabolism and chemical, fatty acid (FA) and amino acid (AA) composition in tissues (longissimus dorsi (LD) and semitendinosus (ST) muscles, heart, spleen, liver and cecum) following the dietary addition of extruded linseed and walnut meal (50:50 mix). Plasma [...] Read more.

We investigated changes in nitrogen metabolism and chemical, fatty acid (FA) and amino acid (AA) composition in tissues (longissimus dorsi (LD) and semitendinosus (ST) muscles, heart, spleen, liver and cecum) following the dietary addition of extruded linseed and walnut meal (50:50 mix). Plasma creatinine and urea nitrogen were determined as well. Two trials were designed using barrows (five replicates) allotted in two groups [(control, (SM) and experimental, (LEW))] in metabolic cages. The experimental diet rich in n-3 FA led to a significant increase of retained nitrogen (>8.09% in the LEW group). The biological value of feed protein was 14.8% higher in the LEW group than in the SM group. LD muscles from the SM group and liver from the LEW group had greater nitrogen contents, whereas the heart and spleen had lower concentrations of nitrogen in the LEW group. Diet had a pronounced effect on n-3 FA, particularly on α-linolenic fatty acid (ALA) (p < 0.0001). The highest levels of ALA were recorded in the cecum (>6.06 times in LEW) and heart (>5.44 times in LEW). The highest level of lysine was noticed in the LD muscle (>2.1% in SM). An n-3-rich diet significantly reduced the amount of nitrogen excreted; greater than 40% nitrogen was retained, thus improving the meat composition. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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14 pages, 527 KiB

Open AccessArticle

Effects of Long-Term Dietary Protein Restriction on Intestinal Morphology, Digestive Enzymes, Gut Hormones, and Colonic Microbiota in Pigs

by Defu Yu, Weiyun Zhu and Suqin Hang

Animals 2019, 9(4), 180; https://doi.org/10.3390/ani9040180 - 20 Apr 2019

Cited by 35 | Viewed by 5239

Abstract

Using protein-restriction diets becomes a potential strategy to save the dietary protein resources. However, the mechanism of low-protein diets influencing pigs’ growth performance is still controversial. This study aimed to investigate the effect of protein-restriction diets on gastrointestinal physiology and gut microbiota in [...] Read more.

Using protein-restriction diets becomes a potential strategy to save the dietary protein resources. However, the mechanism of low-protein diets influencing pigs’ growth performance is still controversial. This study aimed to investigate the effect of protein-restriction diets on gastrointestinal physiology and gut microbiota in pigs. Eighteen weaned piglets were randomly allocated to three groups with different dietary protein levels. After a 16-week trial, the results showed that feeding a low-protein diet to pigs impaired the epithelial morphology of duodenum and jejunum (p < 0.05) and reduced the concentration of many plasma hormones (p < 0.05), such as ghrelin, somatostatin, glucose-dependent insulin-tropic polypeptide, leptin, and gastrin. The relative abundance of Streptococcus and Lactobacillus in colon and microbiota metabolites was also decreased by extreme protein-restriction diets (p < 0.05). These findings suggested that long-term ingestion of a protein-restricted diet could impair intestinal morphology, suppress gut hormone secretion, and change the microbial community and fermentation metabolites in pigs, while the moderately low-protein diet had a minimal effect on gut function and did not impair growth performance. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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17 pages, 1689 KiB

Open AccessArticle

Red Grape Pomace Rich in Polyphenols Diet Increases the Antioxidant Status in Key Organs—Kidneys, Liver, and Spleen of Piglets

by Veronica Sanda Chedea, Laurentiu Mihai Palade, Rodica Stefania Pelmus, Catalin Dragomir and Ionelia Taranu

Animals 2019, 9(4), 149; https://doi.org/10.3390/ani9040149 - 5 Apr 2019

Cited by 34 | Viewed by 5605

Abstract

The aim of this work was to evaluate the influence of a diet containing 5% dried GP on the antioxidant status (total antioxidant status (TAS), antioxidant enzyme activity (catalase-CAT, superoxide dismutase-SOD, and gluthatione peroxidase-GPx), and lipid peroxidation) on the key organs of the [...] Read more.

The aim of this work was to evaluate the influence of a diet containing 5% dried GP on the antioxidant status (total antioxidant status (TAS), antioxidant enzyme activity (catalase-CAT, superoxide dismutase-SOD, and gluthatione peroxidase-GPx), and lipid peroxidation) on the key organs of the liver, kidneys, and spleen in relation to health status as indicated by blood biochemical parameters and total polyphenol content in the blood, organs (liver, spleen, kidney, mesenteric lymph nodes, heart, and brain) and Longissimus dorsi muscle in piglets. The GP diet results in a significant increase of TAS in the liver, spleen, and kidneys, with increased CAT activity in the spleen and kidneys, increased SOD activity in the liver, kidneys, and spleen, and increased GPx activity in the kidneys, as well as a decrease in lipid peroxidation in the liver and kidneys. The GP included in the piglets’ feed contained polyphenols that showed antioxidant activity and were absorbed in the plasma, contributing to maintaining the good health of the animals. The inclusion of 5% GP inclusion in the diets of piglets is beneficial for overall normal blood constituent metabolism and helps to maintain piglet health by increasing the polyphenol content in blood plasma and antioxidant activity in the liver, spleen, and kidneys. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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11 pages, 252 KiB

Open AccessArticle

Effects of Fat Supplementation during Gestation on Reproductive Performance, Milk Composition of Sows and Intestinal Development of Their Offspring

by Xie Peng, Chuan Yan, Liang Hu, Yan Liu, Qin Xu, Ru Wang, Linlin Qin, Cheng Wu, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Yong Zhuo, Jian Li, De Wu and Lianqiang Che

Animals 2019, 9(4), 125; https://doi.org/10.3390/ani9040125 - 28 Mar 2019

Cited by 11 | Viewed by 4014

Abstract

Various fats are used in swine diets as sources of energy and essential fatty acids. Our aim was to evaluate the effects of fat supplementation during gestation on reproductive performance, milk composition of sows and intestinal development of their offspring. Fifty sows were [...] Read more.

Various fats are used in swine diets as sources of energy and essential fatty acids. Our aim was to evaluate the effects of fat supplementation during gestation on reproductive performance, milk composition of sows and intestinal development of their offspring. Fifty sows were randomly allocated into two groups receiving the control (CON) and high-fat diets (HF diet) during gestation. After farrowing, all sows received the same lactation diet and were fed ad libitum until weaning at day 20 of lactation. The results showed that being fed the HF diet did not markedly improve the performance of sows and their offspring. However, the HF diet increased (p < 0.05) the colostrum contents of protein and no-fat solids, and the plasma concentration of prolactin at farrowing. Moreover, piglets born of sows fed the HF diet had higher (p < 0.05) jejunal villous height, as well as deeper (p < 0.05) jejunal and colonic crypt depths compared with piglets born of sows fed the CON diet. In addition, piglets born of sows fed the HF diet had markedly increased (p < 0.05) mRNA abundances of innate immunity-related genes on toll-like receptor 4 (TLR-4), toll-like receptor 9 (TLR-9) and myeloid differentiation factor 88 (MyD88) in ileum compared with piglets born of sows fed the CON diet. These findings indicated that dietary fat supplementation during gestation did not markedly improve the performance of sows and their offspring, but improved colostrum quality and concentration of prolactin on the day of farrowing, associated with modifications of intestinal morphology and innate immunity of their offspring. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

15 pages, 2449 KiB

Open AccessArticle

Health Benefits of Supplementing Nursery Pig Diets with Microalgae or Fish Oil

by Alison V. Lee, Lan You, Se-Young Oh, Ziwei Li, Alexandra Code, Cuilan Zhu, Rebecca E. Fisher-Heffernan, Timothy R. H. Regnault, Cornelis F. M. De Lange, Lee-Anne Huber and Niel A. Karrow

Animals 2019, 9(3), 80; https://doi.org/10.3390/ani9030080 - 5 Mar 2019

Cited by 25 | Viewed by 5245

Abstract

Weaning stress can negatively impact a pig’s performance; dietary supplementation with omega-3 polyunsaturated fatty acids (n-3 PUFA) reduces inflammatory stress and promotes nursery pig’s health and growth. Fish oil (FO) is a major source of n-3 PUFA; however, microalgae (AL) may provide an [...] Read more.

Weaning stress can negatively impact a pig’s performance; dietary supplementation with omega-3 polyunsaturated fatty acids (n-3 PUFA) reduces inflammatory stress and promotes nursery pig’s health and growth. Fish oil (FO) is a major source of n-3 PUFA; however, microalgae (AL) may provide an alternative source of n-3 PUFA. The aim of this study was to assess the health benefits of supplementing a plant protein-based nursery diet with 3.12% AL or 1.25% FO providing equal total n-3 PUFA compared to a control (CON) diet. Seventy-two pigs were fed experimental diets for three weeks (phases 1 and 2), followed by a common standard diet for three weeks (phase 3). Following phase 2, 8 pigs per treatment underwent a lipopolysaccharide (LPS) immune stress challenge to assess the acute-phase response and 8 pigs per treatment were vaccinated with novel antigens to assess acquired immunity. No significant differences in piglets’ growth were observed, despite decreased feed intake in FO piglets compared to AL piglets in phase 3. AL supplementation tended to reduce, and FO supplementation significantly reduced the LPS-induced fever response. The AL pigs had significantly reduced cortisol responses, increased cytokine concentrations, and increased chromogranin A concentrations compared to FO and CON pigs following LPS challenge. Results suggest that AL or FO supplementation in nursery diets differentially modulate the acute-phase response, possibly due to different n-3 PUFA profiles between the two ingredients. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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Review

Jump to: Research

13 pages, 243 KiB

Open AccessReview

Development of Swine’s Digestive Tract Microbiota and Its Relation to Production Indices—A Review

by Damian Knecht, Paulina Cholewińska, Anna Jankowska-Mąkosa and Katarzyna Czyż

Animals 2020, 10(3), 527; https://doi.org/10.3390/ani10030527 - 21 Mar 2020

Cited by 49 | Viewed by 6228

Abstract

The development of research methods and tools related to microbiome investigation, as well as widened knowledge and awareness concerning the significance of microorganisms inhabiting mammalian organisms, has led to an increasing popularity of studies in this field. This review paper presents some issues [...] Read more.

The development of research methods and tools related to microbiome investigation, as well as widened knowledge and awareness concerning the significance of microorganisms inhabiting mammalian organisms, has led to an increasing popularity of studies in this field. This review paper presents some issues related to the swine microbiome, its development starting from an early age of life and its status in adult animals, as well as factors affecting the microbiome in pigs. Attention is paid to the role of probiotics and prebiotics as alternatives to antibiotics in the context of post-weaning diarrhea treatment, and to the role of microorganisms inhabiting the digestive tract of pigs in performance indices formation. In veterinary and pork production practice, understanding of the swine microbiome and its relationships with the host organism may be useful in the prevention of some diseases and also in improvement of performance results of animals. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

18 pages, 331 KiB

Open AccessReview

From Acidifiers to Intestinal Health Enhancers: How Organic Acids Can Improve Growth Efficiency of Pigs

by Benedetta Tugnoli, Giulia Giovagnoni, Andrea Piva and Ester Grilli

Animals 2020, 10(1), 134; https://doi.org/10.3390/ani10010134 - 14 Jan 2020

Cited by 88 | Viewed by 9919

Abstract

Organic acids have been used successfully in pig production as a cost-effective performance-enhancing option and they continue to be the number one alternative to antibiotic growth promoters. The aim of this review is to provide the biological rationale behind organic acids use in [...] Read more.

Organic acids have been used successfully in pig production as a cost-effective performance-enhancing option and they continue to be the number one alternative to antibiotic growth promoters. The aim of this review is to provide the biological rationale behind organic acids use in pig production, focusing on their different effects along the gastrointestinal tract of pigs. Organic acids are reviewed for their antimicrobial properties and for their classic use as acidifiers, with particular attention to pH modulation and microflora control. Additional beneficial effects on intestinal health and general metabolism are presented and we explain the advantage of microencapsulation as a tool to deliver organic acids along the intestine. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

26 pages, 424 KiB

Open AccessReview

Seaweeds in Pig Nutrition

by Carlo Corino, Silvia Clotilde Modina, Alessia Di Giancamillo, Sara Chiapparini and Raffaella Rossi

Animals 2019, 9(12), 1126; https://doi.org/10.3390/ani9121126 - 12 Dec 2019

Cited by 76 | Viewed by 6568

Abstract

Seaweeds are macroalgae, with different sizes, colors and composition. They consist of brown algae, red algae and green algae, which all have a different chemical composition and bioactive molecule content. The polysaccharides, laminarin and fucoidan are commonly present in brown seaweeds, ulvans are [...] Read more.

Seaweeds are macroalgae, with different sizes, colors and composition. They consist of brown algae, red algae and green algae, which all have a different chemical composition and bioactive molecule content. The polysaccharides, laminarin and fucoidan are commonly present in brown seaweeds, ulvans are found in green seaweeds and, red algae contain a large amount of carrageenans. These bioactive compounds may have several positive effects on health in livestock. In order to reduce the antimicrobials used in livestock, research has recently focused on finding natural and sustainable molecules that boost animal performance and health. The present study thus summarizes research on the dietary integration of seaweeds in swine. In particular the influence on growth performance, nutrients digestibility, prebiotic, antioxidant, anti-inflammatory, and immunomodulatory activities were considered. The review highlights that brown seaweeds seem to be a promising dietary intervention in pigs in order to boost the immune system, antioxidant status and gut health. Data on the use of green seaweeds as a dietary supplementation seems to be lacking at present and merit further investigation. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

15 pages, 1037 KiB

Open AccessReview

Nutritional Regulation of Gut Barrier Integrity in Weaning Piglets

by Silvia Clotilde Modina, Umberto Polito, Raffaella Rossi, Carlo Corino and Alessia Di Giancamillo

Animals 2019, 9(12), 1045; https://doi.org/10.3390/ani9121045 - 29 Nov 2019

Cited by 46 | Viewed by 7298

Abstract

Weaning is very stressful for piglets and leads to alterations in the intestinal barrier, a reduction in nutrient absorption and a higher susceptibility to intestinal diseases with heavy economic losses. This review describes the structures involved in the intestinal barrier: the epithelial barrier, [...] Read more.

Weaning is very stressful for piglets and leads to alterations in the intestinal barrier, a reduction in nutrient absorption and a higher susceptibility to intestinal diseases with heavy economic losses. This review describes the structures involved in the intestinal barrier: the epithelial barrier, immune barrier and the enteric nervous system. Here, new insights into the interactions between feed components and the physiology and morphology of the epithelial barrier are highlighted. Dietary strategies focused on improving gut health are also described including amino acids, phytochemicals and organic acids. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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11 pages, 225 KiB

Open AccessReview

Amino Acids Influencing Intestinal Development and Health of the Piglets

by Qi Mou, Huan-Sheng Yang, Yu-Long Yin and Peng-Fei Huang

Animals 2019, 9(6), 302; https://doi.org/10.3390/ani9060302 - 31 May 2019

Cited by 37 | Viewed by 5923

Abstract

The amino acids and other components of diet provide nourishment for piglet intestinal development and maturation. However, early-weaned piglets struggle with tremendous stress, impairing normal intestinal health and leading to intestinal dysfunction and even death. The high prevalence worldwide of post-weaning diarrhoea syndrome [...] Read more.

The amino acids and other components of diet provide nourishment for piglet intestinal development and maturation. However, early-weaned piglets struggle with tremendous stress, impairing normal intestinal health and leading to intestinal dysfunction and even death. The high prevalence worldwide of post-weaning diarrhoea syndrome (PWDS) in piglets has led to much interest in understanding the important role of nutrients in the establishment and maintenance of a functional intestinal tract. In particular, the impacts of amino acids on these functions must be considered. Amino acid levels greatly influence intestinal development in weaning piglets. The lack of amino acids can cause marked structural and functional changes in the intestine. Therefore, a comprehensive understanding of the functions of amino acids is necessary to optimize amino acid requirements of the developing intestinal tract to maximize piglet health and growth performance. This review summarizes the role of specific amino acids (arginine, glutamate, threonine, sulphur-containing amino acids (SCAAs), and branched-chain amino acids (BCAAs)) that have been proven to be beneficial for the intestinal health of weaned piglets. Full article

(This article belongs to the Special Issue Recent Advances in Pig Nutrition)

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