Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Tibetan Sheep
2.2. Immunohistochemistry Detection of HIF-1α, HIF-2α, HIF-3α and VEGF
2.3. Detection of HIF-1α, HIF-2α, HIF-3α and VEG by ELISA
2.4. RT-PCR
2.5. Measurement and Statistical Analysis
3. Results
3.1. Immunostaining for HIF-1α, HIF-2α, HIF-3α and VEGF in the Heart and Lung of Tibetan Sheep
3.2. HIF-1α, HIF-2α, HIF-3α and VEGF ELISA
3.3. RT-PCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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He, Y.; Munday, J.S.; Perrott, M.; Wang, G.; Liu, X. Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep. Animals 2019, 9, 673. https://doi.org/10.3390/ani9090673
He Y, Munday JS, Perrott M, Wang G, Liu X. Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep. Animals. 2019; 9(9):673. https://doi.org/10.3390/ani9090673
Chicago/Turabian StyleHe, Yanyu, John S Munday, Matthew Perrott, Guan Wang, and Xiu Liu. 2019. "Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep" Animals 9, no. 9: 673. https://doi.org/10.3390/ani9090673