In Silico Insights into HIV-1 Vpu-Tetherin Interactions and Its Mutational Counterparts
Round 1
Reviewer 1 Report
The manuscript “In silico insights into HIV-1 Vpu-tetherin interactions and its mutational counterparts” by Patil Sneha, Urmi Shah and S. Balaji describes the differential interaction of host protein Tetherin with HIV-1 Vpu derived from blood and brain. Information provided is of interest to researchers in this filed. The manuscript is well written and easy to follow. Few issues may need to be addressed. They include:
The authors should correct all grammar and spelling errors
Table 9: Please change “Chaufasman” to “Chou-Fasman”
Author Response
All the grammatical and spelling errors have been rectified in the manuscript.
In Table 9, as well as throughout, 'chaufasman' was corrected to 'Chou-Fasman'.
Reviewer 2 Report
please see attached
Comments for author File: 
Comments.pdf
Author Response
The responses to reviewer 2 is attached separately as a word document
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
This manuscript describes a computational study of models of complexes between HIV-1 Vpu and human
tetherin (BST-2). This version of the manuscript is a revision of a resubmission of a previous version.
In the original version, the authors built models of complexes of the transmembrane domain (TMD) α-
helices of Vpu and tetherin aligned parallel with respect to sequence direction. This is unlikely in light
of experimental work. In the resubmission, the authors have generated complex models in which the two
TMD α-helices are anti-parallel. A large portion of the results reported were labelled as H-bond interactions
between mostly hydrophobic residues, which is clearly impossible for α-helices. In this revision, the authors
have admitted this mistake and renamed these as just hydrophobic interactions.
There are no more major mistakes in the modeling results, though their value is somewhat limited. There
are still many mistakes of English grammar in the manuscript that need correction after careful proofreading.
I do not need to see such a revision.
