This study investigates the impact of post-volcanic gas emissions on soil microbial communities in the Băile Lăzărești region (Romania). Nineteen soil samples across a CO
2 gradient ranging from background levels to ≈46,221 ppm. Methane and hydrogen sulfide showed localized peaks (CH
4
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This study investigates the impact of post-volcanic gas emissions on soil microbial communities in the Băile Lăzărești region (Romania). Nineteen soil samples across a CO
2 gradient ranging from background levels to ≈46,221 ppm. Methane and hydrogen sulfide showed localized peaks (CH
4 up to 8271 ppm; H
2S up to ~10.12 ppm), with CH
4 contributing to outlier community patterns. eDNA metabarcoding identified 3064 OTUs, (2463 bacterial and 601 fungal). Bacteria were dominated by Proteobacteria, fungi by Ascomycota, with Thelebolales nearly ubiquitous. Alpha diversity (Chao1, Fisher) declined significantly in high-CO
2 soils (>3000 ppm), while intermediate concentrations (1000–3000 ppm) showed heterogeneous responses. Beta-diversity analyses (PCoA, clustering) revealed distinct grouping of high-CO
2 soils, with sample P16 (CH
4-rich) forming an outlier. A PCA including CO
2, CH
4, and H
2S confirmed CO
2 as the main driver of variance (>65%), with CH
4 accounting for local effects. At the genus level,
Acidobacterium,
Granulicella,
Streptomyces, and
Nocardia increased with CO
2, while
Rhizobium and
Pseudomonas declined. Fungal responses were mixed:
Thelebolus and
Cladosporium increased, whereas
Mortierella and
Cryptococcus decreased. Overall, elevated soil CO
2 reduced microbial richness and reorganized communities, while CH
4 shaped local niches. These findings provide key natural analog insights for assessing ecological risks of CO
2 leakage from geological storage.
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