Cell Stress Reduction by a Novel Perfusion-Culture System Using Commercial Culture Dish
, Atsuhito Okonogi 2, Yohei Anzai 2, Tadataka Kamiyama 2, Keiji Katano 2, Nobuhiko Hoshi 1, Katsuo Mogi 3,* and Tohru Natsume 3,*
Round 1
Reviewer 1 Report
Dear Authors
The research article ´Cell stress reduction by a novel perfusion-culture system using commercial culture dish´ is very innovative and interesting. I find the research details very well explained. However, it is good if this MS includes more study on the different type of cell culture and elaborate more on cell behaviour and proliferation durations if it is also affected.
In various cell culture applications cells are usually cultured in multiple dishes at the same time. The author did mention the one type of cell culture but it also need to be elaborated that how it could be used in multiple culture well requirements. How it will meet the volume requirement of the culture flasks. The current version of the CD-Adapter is not been explained and should be added these details. There are some CD based sensing techniques been reported and I believe that the claim of CD-Adapter is compatible for cell culture is possible. But cell stress defining on basis of marker DDIT3 is not enough it should be more detailed on morphological changes and stress of the cell cultures. The imaging at various stage can be good evidence support for the claim.Overall I find it useful for the readers. Minor proofreading is needed.
Thank you
Author Response
Response to Reviewer 1 Comments
December 3rd, 2019
From: Reiko Yasuda
Graduate School of Agricultural Science, Kobe University
1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan
Tel: +81-78-803-5928
Dear Sir,
We thank you for fruitful suggestions. Your valuable comments helped me very much to improve the quality as well as the readability of the paper. On the basis of your comments, I added new description. I hope the revision could meet the conditions to be considered as the publication.
Author Response File: 
Author Response.docx
Reviewer 2 Report
See attached document
Comments for author File: 
Comments.pdf
Author Response
Response to Reviewer 2 Comments
December 3rd, 2019
From: Reiko Yasuda
Graduate School of Agricultural Science, Kobe University
1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan
Tel: +81-78-803-5928
Dear Sir,
We thank you for fruitful suggestions, especially for suggesting the better figure and sentences. Your valuable comments helped me very much to improve the quality as well as the readability of the paper. On the basis of your comments, I added new description. I hope the revision could meet the conditions to be considered as the publication.
Author Response File: Author Response.docx
Round 2
Reviewer 2 Report
Review report attached
Comments for author File: Comments.pdf
Author Response
Response to Reviewer 2 Comments
December 12th, 2019
From: Reiko Yasuda
Graduate School of Agricultural Science, Kobe University
1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan
Tel: +81-78-803-5928
Dear Sir,
We thank you for fruitful suggestions, especially for suggesting the better figure and sentences. Your valuable comments helped me very much to improve the quality as well as the readability of the paper. On the basis of your comments, I added new description. I hope the revision could meet the conditions to be considered as the publication.
Author Response File: Author Response.docx
