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Applied Sciences
Volume 12
Issue 1
10.3390/app12010216
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Article
Peer-Review Record

Mass Spectrometry Imaging Analysis of Metabolic Changes in Green and Red Tomato Fruits Exposed to Drought Stress

Appl. Sci. 2022, 12(1), 216; https://doi.org/10.3390/app12010216
by Hiroko Asakura 1, Shu Taira 2, Junko Funaki 3, Takashi Yamakawa 4, Keiko Abe 1,5 and Tomiko Asakura 1,*
Reviewer 1: Anonymous
Reviewer 2:
Rafal Ogorek
Appl. Sci. 2022, 12(1), 216; https://doi.org/10.3390/app12010216
Submission received: 12 December 2021 / Revised: 20 December 2021 / Accepted: 21 December 2021 / Published: 27 December 2021
(This article belongs to the Special Issue Modern Molecular Imaging: New Frontiers in Biotechnology)

Round 1

Reviewer 1 Report

This MS reported the localizations of metabolites in fruits 26 by using mass spectrometry imaging. They found that the oleic acid accumulated to high levels in placenta and the biosynthesis of pectin was suppressed under drought stress but increased in seeds. They also proposed that under drought conditions, a shift to phospholipid biosynthesis occurs that protects seeds from dehydration.  Its novelty maybe provide useful information for subsequent research, it is interesting.  In addition, the author writes very seriously, with several minor errors in the reference, please check it.

Author Response

Thank you so much for reviewing our manuscript. According to the reviewer’s comments, we revised manuscript. Prior to describe the revision points, would you explain the submitted manuscript was not final version. After the submitting of first version, assistant manager kindly indicated the error of our references. Therefore, we resubmitted the corrected manuscript. But the manuscript that reviewers read was incorrected manuscript before edition. The manuscript that we submit now is distinctly different from the manuscript to be reviewed by reviewers. We appreciate your understanding. 

Author Response File: Author Response.docx

Reviewer 2 Report

In my opinion, the scope of research and the quality of the preparation of this manuscript are at a good level.That is why I like the work and I have only a few very small comments before it is officially approved for print:

1) all Latin names must be written in curse

2) Fig. 1 – please write the value of the scale bar in the description and remove the value from fig.

Author Response

Thank you so much for reviewing our manuscript. According to the reviewer’s comments, we revised manuscript.

a) All Latin names were written in Italics

b) The value was eliminated from picture and moved to legend of figure 1 

Author Response File: Author Response.docx

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