Antiproliferative Activity of Aminobenzylnaphthols Deriving from the Betti Reaction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures
2.2. Preparation of 1, 2, and Cisplatin Solutions
2.3. Study Design
2.4. In Vitro Cytotoxicity Test
2.4.1. Short-Term Studies: MTT Assay and Trypan Blue Exclusion Assay
2.4.2. Trypan Blue Assay
2.4.3. Long-Term Studies: Clonogenic Assay
2.5. Statistic Treatments
3. Results and Discussion
3.1. Cytotoxicity of 1 and 2 in Human Caco-2 Adenocarcinoma and SH-SY5Y Neuroblastoma Cells
3.2. Mitochondrial Function
3.3. Membrane Integrity
3.4. Colony-Forming Inhibition of Molecules 1 and 2 in Caco-2 Adenocarcinoma and SH-SY5Y Neuroblastoma Cells
3.5. Mechanistic Considerations
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Cell Line | Cisplatin | 1 | 2 |
---|---|---|---|
Caco-2—24 h | 97 | 125 | 94 |
Caco-2—72 h | 88 | 80 | 83 |
SH-SY5Y—24 h | 88 | 80 | 182 |
SH-SY5Y—72 h | 105 | 113 | 93 |
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Mallamaci, R.; Capozzi, M.A.M.; Cardellicchio, C. Antiproliferative Activity of Aminobenzylnaphthols Deriving from the Betti Reaction. Appl. Sci. 2022, 12, 7779. https://doi.org/10.3390/app12157779
Mallamaci R, Capozzi MAM, Cardellicchio C. Antiproliferative Activity of Aminobenzylnaphthols Deriving from the Betti Reaction. Applied Sciences. 2022; 12(15):7779. https://doi.org/10.3390/app12157779
Chicago/Turabian StyleMallamaci, Rosanna, Maria Annunziata M. Capozzi, and Cosimo Cardellicchio. 2022. "Antiproliferative Activity of Aminobenzylnaphthols Deriving from the Betti Reaction" Applied Sciences 12, no. 15: 7779. https://doi.org/10.3390/app12157779