Flocculating Activity of a Bioflocculant from Bacillus megaterium BMBF in Treatment of Domestic and Coal Mine Wastewater
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Media
2.2. Bacterium, Extraction and Purification
2.3. Chemical Compounds
2.4. Characterisation of the Bioflocculant
2.5. Impact of Dosage Size and Metal Ions
2.6. pH and Thermal Stabilities of the Bioflocculant
2.7. Cytotoxicity
2.8. Reduction of Pollutants in Wastewater
2.9. Statistical Analysis
3. Results and Discussion
3.1. B. megaterium BMBF’s Bioflocculant Yield
3.2. Chemical Components
3.3. Characterisation of the Bioflocculant
3.4. Impact of Dosage and Metal Ions
3.5. pH and Thermal Stabilities of the Bioflocculant
3.6. Cytotoxicity
3.7. Reduction of Pollutants in Wastewater
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Components | Composition (%) (w/w) |
---|---|
Proteins | 12 |
Carbohydrates | 27 |
Uronic acid | 61 |
Elements | Composition (%) (w/w) |
---|---|
Potassium | 0.5 |
Oxygen | 17.5 |
Nitrogen | 1.1 |
Carbon | 61 |
Magnesium | 2.8 |
Chlorine | 0.9 |
Phosphorus | 8.1 |
Sodium | 1.7 |
Calcium | 6.4 |
Dosage (mg/mL) | %FE ± SD | Metal Ions | %FE ± SD | pH | %FE ± SD | Temperature (°C) | %FE ± SD |
---|---|---|---|---|---|---|---|
0.2 | 95 ± 2.0 a | Na+ | 59 ± 2.3 a | 3 | 89 ± 1.3 a | 50 | 98 ± 0.0 a |
0.4 | 93 ± 1.5 a | K+ | 75 ± 3.4 b | 4 | 89 ± 3.1 a | 60 | 98 ± 1.0 a |
0.6 | 93 ± 2.5 a | Li+ | 91 ± 0.6 c | 5 | 89 ± 2.5 a | 70 | 96 ± 1.5 a |
0.8 | 92 ± 1.3 a | Mg2+ | 72 ± 3.4 b | 6 | 90 ± 2.0 a | 80 | 96 ± 2.0 a |
Mn2+ | 69 ± 0.6 b | 7 | 99 ± 0.5 a | 90 | 93 ± 0.2 a | ||
Ba2+ | 95 ± 1.5 c | 8 | 95 ± 1.0 a | 100 | 89 ± 0.5 a | ||
Ca2+ | 96 ± 6.1 c | 9 | 93 ± 0.5 a | ||||
Fe3+ | 82 ± 2.8 b,c | 10 | 92 ± 0.5 a | ||||
Control | 71 ± 1.0 b | 11 | 92 ± 2.1 a |
Sample | Bovine Dermis | Vero |
---|---|---|
Bioflocculant | 59 ± 0.8 | 240 ± 2.0 |
5-fluorouracil | 15 ± 0.5 | 25 ± 4.8 |
Flocculants | Local Coal Mine Wastewater | Erwat Wastewater | ||
---|---|---|---|---|
COD | BOD | COD | BOD | |
Bioflocculant | 97 ± 0.3 a | 99.3 ± 2.0 a | 99.2 ± 3.3 a | 93 ± 2.6 a |
Aluminium chloride | 92 ± 2.3 a | 95 ± 1.8 a | 97.2 ± 0.6 a | 80± 1.3 a |
Ferric chloride | 93 ± 1.0 a | 96 ± 0.3 a | 98 ± 1.3 a | 84 ± 0.4 a |
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Selepe, T.N.; Akanbi, R.; Maliehe, T.S.; Moganedi, K.; Masoko, P. Flocculating Activity of a Bioflocculant from Bacillus megaterium BMBF in Treatment of Domestic and Coal Mine Wastewater. Appl. Sci. 2022, 12, 8312. https://doi.org/10.3390/app12168312
Selepe TN, Akanbi R, Maliehe TS, Moganedi K, Masoko P. Flocculating Activity of a Bioflocculant from Bacillus megaterium BMBF in Treatment of Domestic and Coal Mine Wastewater. Applied Sciences. 2022; 12(16):8312. https://doi.org/10.3390/app12168312
Chicago/Turabian StyleSelepe, Tlou Nelson, Remilekun Akanbi, Tsolanku Sidney Maliehe, Kgabo Moganedi, and Peter Masoko. 2022. "Flocculating Activity of a Bioflocculant from Bacillus megaterium BMBF in Treatment of Domestic and Coal Mine Wastewater" Applied Sciences 12, no. 16: 8312. https://doi.org/10.3390/app12168312