Unveiling the Influence of Carbon Nanotube Diameter and Surface Modification on the Anchorage of L-Asparaginase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials, Enzyme and Chemicals
2.2. Characterization Techniques
2.3. Determination of ASNase Isoelectric Point
2.4. MWCNTs Functionalization
2.5. ASNase Immobilization over MWCNT
2.6. ASNase Activity Measurement
2.7. Operational Stability of Immobilized ASNase
2.8. Enzymatic Kinetic Parameters
3. Results and Discussion
3.1. Characterization of MWCNTs
3.2. Determination of ASNase Isoelectric Point
3.3. Effect of pH on ASNase Immobilization onto MWCNTs
3.4. Effect of Contact Time on ASNase Immobilization onto MWCNTs
3.5. Effect of Enzyme Concentration on ASNase Immobilization onto MWCNTs
3.6. Operational Stability
3.7. Kinetic Parameters Determination
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Base Material | Diameter Range (nm) | Length (µm) | Purity (%) | Ash (wt %) | Specific Surface Area Range (m2/g) |
---|---|---|---|---|---|
MWCNT-10 | <10 | >5 | >97 | <3 | 250–500 |
MWCNT-1020 | 10–20 | >5 | >97 | <3 | 100–160 |
MWCNT-2040 | 20–40 | >5 | >97 | <3 | 80–140 |
MWCNT-60100 | 60–100 | >5 | >97 | <3 | 40–70 |
SBET (±5 m2/g) | ||
---|---|---|
MWCNT | F-MWCNT | |
MWCNT-10 | 350 | 408 |
MWCNT-1020 | 104 | 123 |
MWCNT-2040 | 67 | 85 |
MWCNT-60100 | 30 | 33 |
Organism Source | Immobilization Support | Immobilization Method | IY | RRA | Ref |
---|---|---|---|---|---|
Aspergillus versicolor | f-MWCNTs | Physical adsorption | 54% | 100% | [46] |
E. coli | chitosan-tripolyphosphate nanoparticles | Entrapment | - | 72% | [47] |
E. coli | aluminum oxide pellets | Covalent linkage | 85% | - | [48] |
E. coli | silica nanoparticles | Cross-linkage | 95% | - | [49] |
E. coli | f-MWCNTs | Physical adsorption | 99% | 100% | This work |
MWCNT | f-MWCNT | |||
---|---|---|---|---|
Free ASNase (0.3 mg/mL) | Immobilized ASNase | Free ASNase (0.4 mg/mL) | Immobilized ASNase | |
vmax (mM min−1) | 0.044 | 0.017 | 0.043 | 0.010 |
S50 (mM) | 258.0 | 113.6 | 288.3 | 73.8 |
h | 1.7 | 1.6 | 1.6 | 2.1 |
kcat/S50 (mM−1 min−1) | 4.65 | 1.83 | 3.40 | 0.81 |
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Cristóvão, R.O.; Barros, R.A.M.; Pinho, J.G.; Teixeira, L.S.; Neves, M.C.; Freire, M.G.; Faria, J.L.; Santos-Ebinuma, V.C.; Tavares, A.P.M.; Silva, C.G. Unveiling the Influence of Carbon Nanotube Diameter and Surface Modification on the Anchorage of L-Asparaginase. Appl. Sci. 2022, 12, 8924. https://doi.org/10.3390/app12178924
Cristóvão RO, Barros RAM, Pinho JG, Teixeira LS, Neves MC, Freire MG, Faria JL, Santos-Ebinuma VC, Tavares APM, Silva CG. Unveiling the Influence of Carbon Nanotube Diameter and Surface Modification on the Anchorage of L-Asparaginase. Applied Sciences. 2022; 12(17):8924. https://doi.org/10.3390/app12178924
Chicago/Turabian StyleCristóvão, Raquel O., Rita A. M. Barros, João G. Pinho, Lília S. Teixeira, Márcia C. Neves, Mara G. Freire, Joaquim L. Faria, Valéria C. Santos-Ebinuma, Ana P. M. Tavares, and Cláudia G. Silva. 2022. "Unveiling the Influence of Carbon Nanotube Diameter and Surface Modification on the Anchorage of L-Asparaginase" Applied Sciences 12, no. 17: 8924. https://doi.org/10.3390/app12178924