Snapshot-Based Visible-Near Infrared Multispectral Imaging for Early Screening of Heat Injury during Growth of Chinese Cabbage
Round 1
Reviewer 1 Report
This manuscript describes a snapshot-based visible-near infrared multispectral imaging system detecting the early stage of heat injury during growth of Chinese cabbage.
This is a relevant topic making this a valuable manuscript. However, I still have some comments before the manuscript can be accepted.
Q1. Section 3.2 Growth parameter should be moved in Section 2 Materials and Methods to provide sufficient information about the experiment details. In addition, photosynthetic pigment (e.g., leaf chlorophyll content) also should be added as growth parameter since it response to heat stresses.
Q2. The number of measured Chinese cabbage samples should be defined in Section 2.
Q3. More information should be added in Section 4 to make the discussion deepened and to discuss your findings. I don’t understand why the subtitles of 4.2 and 4.3 are same.
Author Response
Dear journal editor and reviewers,
We are very grateful to the editor for your appropriate and constructive suggestions and for proposed correction to improve the paper quality. We also thank for the effort and time put into the review of the manuscript. The editor and reviewers have brought up some good points and we appreciate the opportunity to clarify our work objective. We tried our best to respond the concerns. The major corrections are listed below point by point. The revised texts are highlighted in red color in the revised manuscript.
- Reviewer 1
This manuscript describes a snapshot-based visible-near infrared multispectral imaging system detecting the early stage of heat injury during growth of Chinese cabbage. This is a relevant topic making this a valuable manuscript. However, I still have some comments before the manuscript can be accepted.
- Section 3.2 Growth parameter should be moved in Section 2 Materials and Methods to provide sufficient information about the experiment details. In addition, photosynthetic pigment (e.g., leaf chlorophyll content) also should be added as growth parameter since it response to heat stresses.
Answer: We much appreciate the reviewer’s suggestion. The section 3.2 has been moved into the Materials and Methods section (Line 142 to 160). In this study, we have measured the values of the maximum Rubisco carboxylation efficiency, electron transport for the given light intensity, and maximum rate of trios phosphate to analyze the photosynthetic characteristics during heat stress. Therefore, we have inserted in Section 2 Growth parameters (Line 142 to 160) and the discussion about the photosynthetic characteristics has been inserted in Section 4 (please see Q3) as follow;
“To analyze the heat stress effects, the parameters and photosynthetic parameters obtained from Chinese cabbages grown at different temperature levels were shown in Table 1 and 2. The growth parameters are the average values of each sample group. And, the photosynthetic parameters of Chinese cabbage samples during heat stress are maximum Rubisco carboxylation efficiency, electron transport for the given light intensity, and maximum rate of trios phosphate use. The Bonferroni correction test was conducted to identify the differences of growth parameters among each temperature group.”
Table 1. Average values of major growth parameters of Chinese cabbage grown at 20 °C, 28 °C, and 36 °C for eight days.
|
Temp. (°C) |
Fresh weight (g/Plant) |
Dry weight (g/Plant) |
Number of leaves (/Plant) |
Leaf area (cm2/Plant) |
Leaf length (cm/Plant) |
Leaf width (cm/Plant) |
|
20/16 |
334.2NS |
14.8 NS |
47.0a |
2974 NS |
32.9 NS |
19.0 NS |
|
28/24 |
317.7 NS |
14.7 NS |
42.3ab |
2925 NS |
32.8 NS |
19.3 NS |
|
36/32 |
300.5 NS |
12.7 NS |
40.7b |
2857 NS |
32.6 NS |
18.0 NS |
- NS) Not statistically significant (not distinguished each other)
a-c) Letters showing statistically significant differences among different groups (Bonferroni correction test)
|
Temp. (°C) |
Vcmax (mol/m2/s) |
Jmax (mol/m2/s) |
TPU (mol/m2/s) |
|
20/16 |
181.5a |
176.0a |
87.8b |
|
28/24 |
221.5a |
211.5a |
155.9b |
|
36/32 |
15.4a |
14.7a |
11.3b |
Table 2. Photosynthetic characteristics of Chinese cabbage grown at 20 °C, 28 °C, and 36 °C for eight days
a-c) Letters showing statistically significant differences among different groups (Bonferroni correction test)
Vcmax, maximum Rubisco carboxylation efficiency; Jmax, electron transport for the given light intensity; TPU, maximum rate of trios phosphate use “
- The number of measured Chinese cabbage samples should be defined in Section 2.
Answer: Thank you for comment. As suggested by reviewer, we have inserted into the section 2 (Line 132 to 134) as follow.
“The 24 Chinese cabbage samples were used for each temperature level (20 °C, 28 °C, and 36 °C), respectively; the total number of measured Chinese cabbage samples was 72.”
- More information should be added in Section 4 to make the discussion deepened and to discuss your findings. I don’t understand why the subtitles of 4.2 and 4.3 are same.
Answer: Thank you for pointing this out. We agree with this comment. Therefore, we have discussed and inserted about the photosynthetic characteristics of the Chinese cabbage samples into Section 4 (Line 400 to 404) as follow;
“In table 2, the values of Vcmax, Jmax, and TPU gradually decreased during the heat stress. These values describes the photosynthetic characteristics of the Chinese cabbage samples. Moreover, based on the Bonferroni correction test, they could be distinguished into different groups each other. This indicates that the photosynthetic efficiency of Chinese cabbage decreased by heat stress.”
In addition, the section 4.2 and 4.3 have been merged into the section 4.2.
Author Response File: 
Author Response.docx
Reviewer 2 Report
1. There are no references to information sources [16-19].
2. What kind of seedling lighting was used when growing cabbage?
3. A white Teflon plate (line 251) has a constant reflection coefficient of 99% over the entire operating range of the spectrum? Link [21] does not provide information on this issue.
4. Figure 3: A more generally accepted interval along the abscissa axis should be selected. For example, 450-900 nm or at least 460-880 nm.
5. How do the authors explain the sharp increase in the reflection coefficient in the range of 630-650 nm? (Figure 3)
6. Why are sections 4.2 and 4.3 named exactly the same?
Author Response
Dear journal editor and reviewers,
We are very grateful to the editor for your appropriate and constructive suggestions and for proposed correction to improve the paper quality. We also thank for the effort and time put into the review of the manuscript. The editor and reviewers have brought up some good points and we appreciate the opportunity to clarify our work objective. We tried our best to respond the concerns. The major corrections are listed below point by point. The revised texts are highlighted in red color in the revised manuscript.
- Reviewer 2
- There are no references to information sources [16-19].
Answer: Thank you for comments. Therefore, the references [16-19] have been removed.
- What kind of seedling lighting was used when growing cabbage?
Answer: Thank you for comments. The Chinese cabbage used in this study was grown in the open field. Then, we moved to an extreme-weather growth chamber to apply heat stress. Metal halide lamps and halogen lamps were used as light sources in the chamber.
A white Teflon plate (line 251) has a constant reflection coefficient of 99% over the entire operating range of the spectrum? Link [21] does not provide information on this issue.
Answer: As suggested by reviewer, the order of references has been reorganized. The reference [21] has been changed into [49] (Line 275).
- Figure 3: A more generally accepted interval along the abscissa axis should be selected. For example, 450-900 nm or at least 460-880 nm.
Answer: Thank you for your kind comment. As suggested by reviewer, the range (462 to 862 nm) has been changed into 460 to 880 nm (Line 324).
- How do the authors explain the sharp increase in the reflection coefficient in the range of 630-650 nm? (Figure 3)
Answer: We much appreciate the reviewer’s comment. The multispectral camera used in this experiment consists of two cameras. One camera can measure a wavelength range of 462nm-623nm, and the other camera can measure a wavelength range of 631nm-870nm. Therefore, there is no continuity in the difference in reflectance between the different spectral values obtained from the two cameras. Therefore, to clarify this point, the following sentence has been inserted into the manuscript as follows.
" The SMI camera consists of two cameras, one camera can measure a wavelength range of 462nm-623nm, and the other camera can measure a wavelength range of 631nm-870nm. “. (Line 167 to 169).
- Why are sections 4.2 and 4.3 named exactly the same?
Answer: As suggested by reviewer, the section 4.2 and 4.3 have been merged into the section 4.2.
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
Some small revisions should be needed before publication.
1. Did the data in Table 2 measued synchronously with data in Table 1?
2. I cann't find the Section 4.3. The number of subtitles in Section 4 should be rearranged.
Author Response
Dear journal editor and reviewers,
We are very grateful to the editor for your appropriate and constructive suggestions and for proposed correction to improve the paper quality. We also thank for the effort and time put into the review of the manuscript. The editor and reviewers have brought up some good points and we appreciate the opportunity to clarify our work objective. We tried our best to respond the concerns. The major corrections are listed below point by point. The revised texts are highlighted in red color in the revised manuscript.
- Reviewer 1
Some small revisions should be needed before publication.
- Did the data in Table 2 measured synchronously with data in Table 1?
Answer: Yes. The data in Table 1 and 2 has been acquired on the same day. After the hyperspectral images of Chinese cabbages were obtained, and then their growth and photosynthetic parameters have been measured, respectively.
- I can't find the Section 4.3. The number of subtitles in Section 4 should be rearranged.
Answer: Thank you for kind comment. The number of subtitles in Section 4 has been reorganized (Line 429 and 444)
Author Response File: Author Response.docx
