1. Introduction
First introduced to the market in 1993, mineral trioxide aggregate (MTA) is developed for root repair and can be used for both non-surgical and surgical interventions, such as root-end filling, vital pulp therapy, pulp capping, root, and furcation perforation repair, and apexification. The composition of MTA materials includes tricalcium silicate, dicalcium silicate, calcium sulfate dihydrate, calcium aluminoferrite, and tricalcium after mixing raw materials of MTA [
1]. MTA is derived from Portland cement, and the main difference is the presence of bismuth oxide and lack of potassium in MTA [
2]. Although similar in some characteristics, MTA and Portland cement are unlike as MTA products have comparatively smaller particle sizes and include fewer heavy metals, with implications of lesser inflammatory and allergic reactions [
3]. However, a previous in vitro study on the biocompatibility of MTA and Portland cement indicated similar cytotoxic effects on cells and failed to detect DNA damage after exposure to the tested treatment conditions [
4,
5]. Minimal inflammatory response and bone healing were demonstrated in vivo, with a similar response and good toleration towards MTA and Portland cement [
6]. Considering the successful results of several investigations, Portland cement can be rendered as a reasonable alternative for MTA in permanent teeth with the advantage of its low cost [
7,
8].
With the aim of overcoming the disadvantages of MTA, a wide range of bioactive cements with variations in composition are introduced to the market, offering similar properties to MTA with fewer drawbacks [
1]. Among MTA-based materials, ProRoot MTA was the first available MTA product, which was followed by MTA Angelus with a reduced setting time due to its decreased calcium sulfate concentration [
9]. Nowadays, various MTA materials are offered in the market with different qualities regarding their setting time, color properties, or ease of handling to enhance their clinical properties. Medcem MTA (Medcem GmbH, Weinfelden, Switzerland) is a second-generation MTA that comprises Pure Portland Cement (Medcem GmbH, Weinfelden, Switzerland) with a color-stable X-ray contrast media, zirconium oxide, according to the product manual. Pure Portland Cement is similar in content except for an additional X-ray opaque ingredient, with a slightly higher hardness according to the manufacturer. Well-Root ST (Vericom, Chuncheon-si, Gangwon-Do, Republic of Korea) is a novel bioceramic root canal sealer presented in the form of a premixed injectable bioactive paste with ease of use. Recently, it was reported that Well-Root ST is an appropriate alternative to ProRoot MTA and Biodentine due to its superior angiogenesis potential as indicated by better vascularization [
10]. There are still limited data available on its properties and its interactions in the scientific literature. BIOfactor MTA (Imicryl Dental, Konya, Turkey) is another bioactive material consisting of tricalcium silicate without bismuth but includes ytterbium oxide as a radiopacifier.
The wide range of choices poses a challenge to clinicians as it is difficult to recognize the differences between these products. Hence, extensive investigations into their specific characteristics and behaviors may improve the understanding of the biological properties of these materials to give some insight to the clinician when making a valid clinical decision. To the best of our knowledge, there is no study available comparing the aforementioned bioactive MTA-based materials in terms of their biocompatibility and osteo/odontogenic differentiation potential. Therefore, the main aim of the present study was to evaluate the cell viability and osteo/odontogenic responses of MSCs derived from tooth germs when exposed to various MTA-based materials. Two null hypotheses were tested: (1) MTA-based materials would not interfere with the viability of stem cells and (2) all tested bioactive materials would support osteo/odontogenic differentiation of stem cells similarly.
2. Materials and Methods
Impacted third molars were obtained from porcine sources after ethical clearance (HDK-2016/39) of the Animal Ethics Committee of Acibadem University. Isolation of MSCs from germ tissues was performed as described previously [
11]. Established MSCs were incubated in the growth medium which comprised MEMα (Gibco, Grand Island, NY, USA), 10% FBS (Gibco, Grand Island, NY, USA), and 100 u/mL Penicillin-Streptomycin-Amphotericin (PSA) solution (Pan Biotech, Aidenbach, Germany) at 37 °C, in a 90% humidified atmosphere of 5% CO
2. Cells were sub-cultured until 3rd passage to be used in the experiments and the medium was changed every other day. Flow cytometry was used to analyze the surface antigen profiles of the cells and data were reported previously [
12].
2.1. Preparation of Material Extracts
Five bioactive materials, including Medcem MTA, MTA Angelus, BIOfactor MTA, Well-Root ST, and Pure Portland Cement were tested in the present study (
Table 1). MTA materials and Pure Portland Cement were prepared by mixing powder and liquid according to manufacturers’ instructions while Well-Root ST did not require any preparation as it was pre-mixed supplied in syringes. Samples were prepared as discs 5 mm in diameter and 3 mm thick in pre-sterilized Teflon
® molds in a laminar flow under aseptic conditions using the manufacturer’s recommendations. Material extracts were prepared as previously described [
13]. All prepared samples were incubated at 37 °C for 6 h. Samples were exposed to UV light at room temperature for 1 h. After sterilization, sample discs were submerged into 1 mL of growth medium and kept in a 90% humidified atmosphere of 5% CO
2 for a day. The next day, each collected extract was filtered through sterile filters with 0.22 µm pores. The extract containing media was diluted 1 to 10 with growth medium for each experiment.
2.2. Cell Viability Assay
Cells were seeded at 10,000 cells/well for 96-well plates in 200 µL of growth medium. Plates were incubated in an incubator at 37 °C with 5% CO2 overnight for cell adherence. The next day, MSCs were exposed to 200 µL of extract solutions diluted in growth medium, and a control group was included with cells incubated only in growth medium. An osteogenic group was determined for the positive control of osteo/odontogenic differentiation in which the cells were cultured in osteogenic medium (DMEM with low glucose containing 10% FBS, 1% PSA, 50 μM ascorbic acid, 10 nM dexamethasone, and 10 nM ß-glycerophosphate). CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS, Promega, Madison, WI, USA) was used to measure cell viability according to the manufacturer’s instructions on days 1, 3, and 7 of cell culture. Absorbance was determined with an ELISA Plate Reader (Varioskan LUX, Thermo Fisher Scientific, Waltham, MA, USA) at 490 nm. The reading of the control group was adjusted as 100% to calculate the viability in test groups accordingly. Each group was worked in triplicates.
2.3. Live/Dead Assay
The LIVE/DEAD® Viability/Cytotoxicity Kit (Thermo Fisher Scientific, USA) for mammalian cells was used to assess the viability of cells. This assay determines cell functions of live and dead cells by staining intracellular esterase activity with green fluorescent calcein-AM and loss of plasma membrane integrity with red-fluorescent ethidium homodimer-1. MSCs were seeded at 20,000/well in 24-well plates in growth medium and incubated overnight. The next day, cells were treated with extract solutions, growth medium, and osteogenic medium as controls. All media was refreshed twice a week. Viability was assessed on days 1, 3, and 7 of cell culture with prepared reagent solutions according to the manufacturer’s instructions. Stained cells were observed under the fluorescence microscope (Nikon, Eclipse TC100, Tokyo, Japan).
2.4. Alkaline Phosphatase Activity
Cells were seeded at 20,000 cells/well in 24-well plates in growth medium and incubated overnight to allow cell adhesion. After 24 h, cells were incubated with extract solutions, and control groups were also determined with osteogenic and growth medium. All media was refreshed twice a week. ALP activity was measured with SigmaFast p-nitrophenyl phosphate tablets (Sigma-Aldrich, St. Louis, MO, USA) on days 1, 3, and 7 of incubation. After washing of cells, the active solution was added to the wells, and the plates were incubated at room temperature in the dark for 1 h. Absorbance was measured with an ELISA Plate Reader (Varioskan LUX, Thermo Fisher Scientific, USA) and readings were converted to moles with a calibration curve set with known moles of ALP.
2.5. Quantitative Real-Time PCR
Total RNA from cells treated with each material was isolated by using GeneJet RNA Purification Kit (Thermo Scientific, Vilnius, Lithuania) on days 1, 3, and 7 of cell culture. Following RNA measurement, cDNA was synthesized with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) following the manufacturer’s instructions. Relative gene expression levels for Runt-related transcription factor 2 (RUNX2), Dentin matrix protein-1 (DMP-1), and Dentin sialophosphoprotein (DSPP) were determined according to the Maxima SYBR Green Master Mix (Thermo Scientific, Waltham, MA, USA) protocol. Β-actin was used as the reference housekeeping gene to normalize data.
Table 2 presents the used primer sequences. Every sample was worked in triplicates. Real-time PCR experiments were performed by using CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ΔCT was determined as the difference between the CT of each marker and ß-actin. ΔΔCT was calculated as the difference of the ΔCT value of the calibrator sample and the ΔCT value of the test sample. The resultant data are given as the relative mRNA expression level, calculated as the fold change (2
−ΔΔCT).
2.6. Statistical Analysis
Statistical significance was analyzed using one-way analysis of variance (ANOVA) with Tukey’s pairwise comparison tests. Statistical analysis was performed with statistical software (GraphPad Prism version 5.0; La Jolla, CA, USA). The p-value less than 0.05 was considered statistically significant for all statistical analyses.
4. Discussion
The biocompatibility of bioactive materials used in vital pulp therapies or repair of dentinal tissue in perforation sealing and retrograde filling is a determining factor in the success of the treatment outcome as the irritation to surrounding tissues can delay wound healing. As well as its biocompatibility, the biomaterial should have osteo/odontogenic induction properties to support differentiation of peripheral potent cells and initiate mineralization. Therefore, in the current study, MSCs were treated with different bioactive MTA-based material extracts diluted in the growth medium and were compared against osteo/odontogenic-induced and non-induced MSCs.
Dental stem cells have been extensively investigated in terms of multiple lineage differentiation and demonstrated great potential in the hard tissue engineering applications of the oral region, such as the healing of bone defects or supporting cell-based pulp-dentin complex regeneration [
14]. Isolated from the tooth germs of third molars, which can be considered a more primitive tissue present during young adulthood compared to more developed pulp and apical papilla tissues, tooth germ-derived MSCs are multipotent stem cells that can benefit from interactions between ectoderm and mesoderm as they are derived from epithelial cells and the underlying mesenchyme [
15]. Recent studies reported a high osteo/odontogenic potential for tooth germ-derived MSCs both in vivo and in vitro, alone or in combination with scaffolds [
11,
13,
16]. Hence, the present study used tooth germ-derived MSCs to demonstrate if MTA-based biomaterials can support the induction of odontogenesis and maintain viability. This investigation was able to demonstrate that tooth germ-derived MSCs can be induced with MTA-based biomaterials with comparable results to osteo/odontogenic medium-induced cells, as indicated by the elevated ALP activity and osteo/odontogenic marker genes with varying degrees.
All test materials exhibited significant cell viability as indicated by comparable results in test groups with the controls via MTS and live/dead assay. Immediate exposure to extracts did not cause cytotoxicity to MSCs, but Medcem MTA demonstrated less viability initially which was compensated over the 7-day cell culture as the cells were able to proliferate. As the decrease in cell viability was not more than 30%, none of the materials were considered cytotoxic according to ISO-10993-5. Medcem MTA is composed of Pure Portland Cement with zirconium oxide added as the opacifier. Interestingly, zirconium oxide is reported to have a better biological response than bismuth oxide, which has been involved in the cytotoxicity shown by some MTA materials [
17]. In the present study, Angelus MTA contains bismuth oxide as the opacifier, which demonstrated no inhibitory effects on the cell viability of MSCs.
Various MTA materials were tested on bone marrow-derived MSCs and were demonstrated to be non-toxic, but they could affect tooth-derived stem cells in a concentration and time-dependent manner [
18]. Studies indicate that MTA materials can promote the proliferation and survival of MSCs derived from dental tissues including apical papilla, exfoliated deciduous teeth, and dental pulp [
19]. Despite minor differences, some behavioral tendencies must be acknowledged among MTA materials. MTA Angelus has been studied for a longer period and has established high biocompatibility with similar results to control medium with different types of cells including dental pulp stem cells (DPSCs), periodontal ligament fibroblasts, and osteoblasts [
20,
21]. Another study reported that undiluted exposition to MTA Angelus increased cell proliferation [
22], in accordance with our results which indicated higher proliferation on day 7 with the MTA Angelus group compared to that of the control group. The difference between studies can be attributed to the dilution of the MTA extract or the growth rate of the cell type, which may be comparably higher in stem cells than in fibroblasts [
20]. Cell viability is reported to be enhanced two- to three-fold with Pure Portland Cement in DPSCs, at days 3 and 7 compared to cells cultured in control media, which confirms the finding of the present study [
23]. When MSCs were previously exposed to Well-Root ST, cell viability was found to be similar on days 1 and 7 but significantly increased compared to the control group on day 3, which is in accordance with our findings [
10]. Culturing human periodontal ligament stem cells on Well-Root ST indicated favorable cell attachment and proliferation potentially due to both chemical composition and the smooth surface of the material [
24]. There were no recent reports on stem cell viability when treated with BIOfactor MTA and Medcem MTA. However, it should be noted that osteogenically induced MSCs performed better over the 7-day incubation with a consistent increase in proliferation and were significantly promoted on day 7 in comparison to all tested materials, suggesting cell viability may be more prone to change over a longer course of exposure with the MTA-based materials. Additionally, several factors that may cause disparities between studies need to be considered while interpreting cellular response to MTA-based materials, such as the type of tested cells, cytotoxicity assessment methods, concentration, and exposure type of the tested materials. Despite minor variances between tested materials, the null hypothesis that all tested MTA-based materials would not interfere with the viability of the stem cells was accepted.
The early phase of osteoblast differentiation involves an increase in ALP activity which reflects the number of osteogenically committed progenitor cells. A high correlation between ALP induction during in vitro osteogenic differentiation and in vivo bone-forming capacity is demonstrated both in enzyme activity and in mRNA expression [
25]. A material with a faster osteogenic induction during early exposure may be more favorable for regenerative pulp therapies as it would immediately stimulate a mineralization barrier. ALP activity results indicated no difference among the tested materials on the immediate exposure measured on day 1. However, Pure Portland Cement exhibited elevated ALP activity on day 3 which continued until the end of cell culture with a significant increase compared to the control group, which may indicate its potential for maintaining osteo/odontogenic activity.
The capacity of the tested materials to induce differentiation was further evaluated with the expression of osteogenic and odontogenic gene markers, RUNX2, DMP-1, and DSPP. RUNX2, a major transcription factor in mineralization, is highly expressed through all stages of craniofacial bone development in osteogenic mesenchyme [
26]. RUNX2 directs MSCs to differentiate towards osteoblast lineage and supplies immature osteoblasts in forming immature bone, while downregulation is necessary for mature osteoblasts in their terminal differentiation [
27]. RUNX2 was significantly upregulated in MSCs cultured in osteogenic media as expected on both day 3 and day 7, which was used as a positive control of the osteogenic differentiation capacity of used cells. Only Medcem MTA indicated significant upregulation of RUNX2 starting on day 3; however, all other tested materials also promoted RUNX2 expression after 7 days in accordance with previous studies [
18], indicating their commitment towards osteogenic differentiation. Interestingly, Pure Portland Cement-exposed MSCs demonstrated a nearly six-fold increase in RUNX2 expression, reaching the highest level among all groups. Pure Portland Cement was reported to express higher levels of RUNX2 and elevated ALP activity in DPSCs compared to that of MTA and the control group, which also confirms the findings of the present study to suggest Pure Portland Cement may have an enhanced osteogenic capacity [
23]. On the other hand, RUNX2 is not specific to osteogenic differentiation, as the protein is detectable in the nuclei of pre- and immature odontoblasts during the early stages of tooth development and regulates both tooth and bone-related expressions [
27].
The most commonly evaluated phenotypic markers for odontogenic differentiation are DMP-1 and DSPP. DMP-1 acts as an inducer in the odontogenic differentiation of pulp cells whereas DSPP is a non-collagenous protein highly expressed in the dentin matrix and regarded as the terminal differentiation marker of odontoblasts [
28]. Overexpression of RUNX2 in odontoblasts inhibits their terminal differentiation and reduces DSPP expression, which indicates RUNX2 is able to induce transdifferentiation of odontoblasts into osteoblasts and needs to be inhibited during odontoblast differentiation to promote normal cell maturation and dentinogenesis [
29]. Although these interactions overlap and cannot be singled out as a specific marker of osteogenesis or odontogenesis, a predisposition in MSCs towards osteogenic or odontogenic differentiation with the tested materials can be noted. In the present study, RUNX2 and DSPP expression levels were different between tested materials and osteogenic medium. MTA Angelus showed upregulation of DSPP after 7 days, unlike osteogenic medium, whereas RUNX2 was highly expressed in both groups, which confirms the findings of a similar study with DPSCs [
30]. Among the tested biomaterials, Medcem MTA demonstrated significant upregulation of DMP-1 on both time points while BIOfactor MTA also indicated a significant increase on day 7. Regarding DSPP expression, MTA Angelus responded with a significant increase early in cell culture whereas Medcem MTA demonstrated a dramatic increase on day 7. In a previous report when MSCs were seeded on MTA-coated cell plates to assess odontogenic differentiation, ALP activity was decreased but relative DSPP gene expression was upregulated [
31]. The low ALP activity may be associated with decreased cell viability, possibly due to the direct exposure of cells to MTA; nevertheless, the cells were able to demonstrate odontogenic differentiation capabilities. In the present study, the cells treated with Well-Root ST and Pure Portland Cement responded with a lesser increase in DSPP expression. Tested MTA materials resulted in upregulation of odontogenic gene markers DMP-1 and DSPP whereas Pure Portland Cement and the osteogenic differentiation control group indicated higher ALP levels in addition to upregulation on RUNX2 expression. Although it is not conclusive, this may suggest a predisposition of MTA materials to direct cells towards odontogenic differentiation while the osteogenic medium may promote cells towards osteogenic lineage. Minor differences in gene expression and cell viability between different formulations of tested MTA materials provide little evidence that one material is superior than the other. Therefore, the second null hypothesis that all tested bioactive materials would support osteo/odontogenic differentiation of stem cells similarly was partially accepted.
As with all in vitro studies, the major limitation lies in the lack of mimicking host reactions to environmental stimuli compared to animal and human studies. Although the biological properties and potential of bioactive cements, like the ones tested in the present study, have been investigated in numerous studies, there are new materials introduced to the market in accordance with high demand. Updated biological profiling of these materials is necessary before their vast clinical use. Hence, it is crucial to test biocompatibility and bioactivity before moving to in vivo investigations and clinical trials to elucidate underlying mechanisms and cell behaviors. The use of standardized procedures such as cytotoxicity assessment according to ISO-10993-5 guidelines and relative gene expression profiles calculated with 2−ΔΔCT ensure reporting reliable results. However, an important limitation of the present study may be due to the lack of a long-term incubation time to demonstrate mineralization and also to determine if the cells can maintain their reported properties in the presence of the tested materials. Nevertheless, the demonstrated properties of the tested materials in the present study may serve as a preliminary evaluation of their potential use, which can further be developed as a biomaterial–cell combination, a potential strategy for many applications in regenerative pulp therapies and root repair.