An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays

Shi, Danxia; Xu, Wenliang; Wong, Marie; Popovich, David G.

doi:10.3390/app13085067

Open AccessCommunication

An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays

by

Danxia Shi

^1,2,

Wenliang Xu

¹,

Marie Wong

³ and

David G. Popovich

^1,*

¹

School of Food and Advanced Technology, College of Sciences, Massey University, Palmerston North 4442, New Zealand

²

Alpha-Massey Natural Nutraceutical Research Centre, Massey University, Palmerston North 4442, New Zealand

³

School of Food and Advanced Technology, Massey University, Auckland 0745, New Zealand

^*

Author to whom correspondence should be addressed.

Appl. Sci. 2023, 13(8), 5067; https://doi.org/10.3390/app13085067

Submission received: 21 February 2023 / Revised: 14 April 2023 / Accepted: 16 April 2023 / Published: 18 April 2023

(This article belongs to the Special Issue Recent Advances in Applied Microbiology and Food Sciences, Volume II)

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Abstract

The MTT antibacterial assay is an important method in natural antibacterial component discovery. Researchers can use the MTT antibacterial assay to quickly and efficiently evaluate the antibacterial activity of natural compounds. The aim of this research was to find a method to reduce background colour and bacterial cell interference when using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to study the antibacterial effect of phytochemicals. This study used NZ ‘Hass’ ripe avocado seed extract as an example to develop a silica gel column chromatography method that could combine with the MTT assay to remove the background colour and bacterial cells before the formazan measuring stage. This method is particularly suitable for MTT-based antibacterial inhibition studies when the tested phytochemical extracts have an interfering colour.

Keywords: background colour; bacterial cell; MTT; silica gel column chromatography method; formazan

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MDPI and ACS Style

Shi, D.; Xu, W.; Wong, M.; Popovich, D.G. An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays. Appl. Sci. 2023, 13, 5067. https://doi.org/10.3390/app13085067

AMA Style

Shi D, Xu W, Wong M, Popovich DG. An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays. Applied Sciences. 2023; 13(8):5067. https://doi.org/10.3390/app13085067

Chicago/Turabian Style

Shi, Danxia, Wenliang Xu, Marie Wong, and David G. Popovich. 2023. "An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays" Applied Sciences 13, no. 8: 5067. https://doi.org/10.3390/app13085067

APA Style

Shi, D., Xu, W., Wong, M., & Popovich, D. G. (2023). An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays. Applied Sciences, 13(8), 5067. https://doi.org/10.3390/app13085067

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays

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