Application of the Drosophila melanogaster Research Model to Evaluate the Toxicity Levels between Lead and Copper
, Stroia Ciprian 4, Morna Anamaria Aurelia 5, Vicas Laura-Gratiela 6 and Ganea Mariana 6Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThe manuscript ``Application of the Drosophila melanogaster research model to evaluate the toxicity levels between lead and copper. `` by Sarac Ioan et al., is adequately organized in a logical and thoughtfully manner. The work may be published after incorporating these few comments/suggestions:
- Have you done experiments with the combined effect of lead and copper? If you did only the separate influence of these two metals then in the title the word ``between`` should be changed.
- Please pay attention to the numbering of the mentioned references in the text. After number 19 you go to 25 (Line 72), then from 26 to 31 (75), then first 65 before 63 and 64 (line 134) and so on.
- Are all 98 references necessary? Please shorten your reference list.
- If it is not prescribed by the journal, I think it is simpler to name the Figures and Tables in order, for example Figure 1 instead of Figure 2.1, Figure 2 not a Figure 2.2. Same for the Tables, Table 1, Table 2 instead of Table 3.1.; Table 3.2. and so on.
- What is the difference between larvae a2 and a3 in the Figure 2.2?
- Please check the equation at Line 211. Should it be in parentheses (100- number experimental group/number of control group)?
- In Tables 3.1; 3.2 and 3.3, f you have the data, what was the value of F critical?
- Also, the mark `` *`` in the Tables acts as a multiplication sign and not a footnote *p<0.05
- In Line 302 and 303, w and vg write in italics if it is written in italics everywhere. Please check the text for the uniformity of writing abbreviations and others.
- In Line 453, 6. Patent is this redundant or the text in manuscript was missing.
Comments on the Quality of English LanguageMinor editing of English language required.
Author Response
Please see attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for Authors1. Avoid punctuation at the end of headings and subheadings unless absolutely necessary. "title.", "1. Introduction.", "5. Conclusions."
2. The author in the manuscript and the information produced on the system do not match, please confirm.
3. In the manuscript, the ranking of authors with equivalent contributions is unusual compared to common paper manuscripts, please confirm.
4. Start with a strong, clear opening statement about the importance of studying metal toxicity. It highlights the impact of heavy metals on environmental and human health and provides specific quantitative data to set a compelling context for the study.
5. Expand on a brief overview of the toxicity mechanisms of lead and copper, citing their key studies demonstrating their biological effects in various models.
6. Explain why Drosophila melanogaster is an ideal model for studying metal toxicity. Discuss the genetic similarity to humans, the rapid life cycle, and the ease of genetic manipulation.
7. Clarify the unique academic value that your research provides. It involves a specific comparison of genetic responses to metal exposure, which has not been thoroughly explored in previous studies.
8. Punctuation throughout the manuscript is extremely casual, with the author's unit ending in addition to the titles and subtitles already mentioned. Please strictly check the writing details of the manuscript!
9. The biggest problem of this manuscript is the lack of mechanism exploration, and the research content and results are phenomena. Then in the discussion section, we need to briefly explain the mechanism behind it and draw a diagram.
Comments on the Quality of English Language1. Simplification of complex sentences so that they are accessible to readers outside the immediate field of genetic toxicology.
2. Organise your content into well-structured paragraphs. Each paragraph should make one main point, starting with a topic sentence followed by sentences that develop that point.
3. Use transitional phrases to ensure a logical flow between sentences and paragraphs.
Author Response
Please see attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Comments and Suggestions for AuthorsThere are still more writing problems throughout the text, e.g., in line 74, Drosophila melanogaster has been abbreviated to D.m., but the full name still appears several times later in the text. Please be sure to check the text again to eliminate this situation.
Author Response
Please see attachment.
Author Response File: Author Response.pdf
