Cellular Basis of Adjuvant Role of n-3 Polyunsaturated Fatty Acids in Cancer Therapy: Molecular Insights and Therapeutic Potential against Human Melanoma

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

Overall, this is a well-researched and informative review article that makes a compelling case for the potential therapeutic utility of n-3 PUFAs as an adjuvant approach in the management of melanoma and other malignant cancers. The authors have done a commendable job in synthesizing the existing knowledge and highlighting the promising avenues for future research in this field.

 

l  some sections, such as molecular mechanisms of omega-3 fatty acids in cancer, could be slightly more condensed to maintain a balanced focus throughout the article.

l  Discuss the safety, dosage, and side effects of n-3 PUFAs in the context of cancer therapy.

Comments on the Quality of English Language

None

Author Response

Dear reviewer 1,

Please see the attachment. 

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The review of Rojas-Solé and co-authors is about the potential role of n-3 PUFAs supplementation as a novel complementary approach to the treatment of malignant cancer such as melanoma.

The current review is an interesting work and I certainly recommend accepting it for publication in applied sciences.

I have a few questions and suggestions. From the entire review, one thing is clear the fatty acids, such as n-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic and eicosapentaenoic acids, exert various physiological effects on melanoma and have ability to inhibit cancer metastatic. However, the review lacks discussion section and future directions.

Use the MDPI template to facilitated the comments and suggestions 

Moreover, I have noted following typos and omissions

Pag. 2 There are also radiotherapy and chemotherapy (remove and radiotherapy).

Pag. 8 Place continuous section number (3.3.1?)

Pag. 9 Wang et al. [51] remove (Wang, et al. 2019)

Pag. 11 y 12 Change H2O2 for H₂O₂

Pag. 13 Change As2O3 for As₂O₃

 

 

 

Author Response

Dear reviewer 2,

Please see the attachment. 

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

The review presents an interesting theme from a scientific standpoint. The article is well-written, but I believe that some points that would be important for a review article are missing. From my perspective, the review could be accepted with modifications. 

-In the introduction, there is a lack of description of the physicochemical properties of the DHA and AA molecules. The authors should provide spectroscopic information to familiarize readers with the molecules under study.

-The manuscript lacks figures to illustrate results. The authors should seek figures so that readers can visualize some of the results.

-As it is a topic of relevance, it would be interesting for the authors to provide results on molecular interaction mechanisms. The authors should survey the interaction sites of key proteins in the processes and important molecular interactions. This would include theoretical computational work. This inclusion will make the manuscript more comprehensive for readers.

-The authors should include in the supplementary material figures of the molecular structures mentioned in the manuscript and highlight important active sites.

-The pharmacokinetic characteristics of DHA and AA were not mentioned. There are webservers to obtain these properties in silico (AdmetSARS2, SwissAdmet, and PREadmet). The authors should include this fundamental information in the manuscript.

Comments on the Quality of English Language

Minor editing of English language required

Author Response

Dear reviewer 3,

Please see the attachment. 

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

This is a well-written and informative review on the topic of omega-3 polyunsaturated fatty acids (n-3 PUFA) and their potential health benefits, particularly regarding cancer. The review covers a broad range of topics related to n-3 PUFA, including their biological functions, dietary sources, and potential health benefits in cancer. The section on the molecular mechanisms of n-3 PUFAs in cancer is particularly detailed and informative. Overall, the review is well-supported by references to scientific studies.
I would suggest some changes mentioned.
1. As the authors described about PUFA and its role, a small section on Nutrigenomics and how PUFA interacts with the genes would be a good thing to strengthen PUFAs role.
2. There are different pathways associated with cancer, it would be good if author can discuss the crucial pathway where n-3 PUFA has been seen to be actively associated.
3. Try to include clinical trials tables investigating the effects of n-3 PUFA supplementation on patient outcomes. Try analysing PMID 34843864.
4. n-3 PUFA and its role in various melanomas can be presented as a figure.

Comments on the Quality of English Language

not applicable 

Author Response

Dear reviewer 4,

Please see the attachment. 

Author Response File: Author Response.pdf