Changes in Skeletal Muscle Atrophy over Time in a Rat Model of Adenine-Induced Chronic Kidney Disease

Abstract

This study evaluated changes over time in skeletal muscle atrophy, expressions of skeletal muscle anabolic and catabolic genes, and mitochondrial activity by skeletal muscle type in an adenine-induced chronic kidney disease (CKD) model. A CKD model was successfully established by feeding male Wistar rats a 0.75% adenine diet for 4 weeks starting at 8 weeks of age. Control and CKD groups were sacrificed at 12 and 20 weeks of age. The back muscles were analyzed histologically, and succinate dehydrogenase (SDH) staining was performed to evaluate mitochondrial activity. Gene expressions of myogenic determination gene number 1 and myogenin as indicators of muscle anabolism, atrogin-1 and muscle RING-finger protein-1 (MuRF1) as indicators of muscle catabolism, and peroxisome proliferator-activated receptor-γ coactivator-1-α as a marker of mitochondrial biogenesis were assessed. Type I and type II muscle cross-sectional areas (CSAs) were decreased at 12 weeks, but type I muscle CSA was recovered at 20 weeks. SDH staining was lower in CKD than in control rats at 12 weeks, but no significant difference was observed at 20 weeks. Increased expressions of myogenin, atrogin-1, and MuRF-1 were observed only at 12 weeks, but no differences were observed at 20 weeks. The adenine-induced CKD rat model appears to show changes in muscle atrophy over time.

1. Introduction

Chronic kidney disease (CKD) is widely recognized as a global public health problem [1], with an estimated global prevalence of 13.4%, which is expected to increase as the population ages [2]. In addition to decreased renal function and increased vascular calcification [3], CKD is associated with muscle wasting [4] and decreased physical function, which contributes to lower quality of life and increased mortality in patients with CKD [5]. Therefore, it is important to prevent and treat skeletal muscle atrophy and the decline in physical function associated with CKD. However, there are no established methods for the prevention or treatment of skeletal muscle atrophy or loss of physical function due to CKD.

Several factors are thought to contribute to skeletal muscle atrophy in CKD [6]. Most notably, an altered balance between catabolism and anabolism regulates skeletal muscle homeostasis [4]. Avin et al. reported that muscle catabolism-related genes, muscle RING-finger protein-1 (MuRF-1), and atrogin-1 are increased in CKD rats [7]. In addition, it has recently been reported that mitochondrial dysfunction, which is thought to provide energy for physical function, is one of the factors contributing to the decline in physical function in CKD [8]. However, how skeletal muscle atrophy changes over time in CKD and how factors related to muscle catabolism and anabolism and mitochondrial activity change over time are unclear.

Several preclinical models of CKD have been used to study skeletal muscle atrophy in CKD, including nephrectomy [9], Cy/+ rats with polycystic kidney disease [7,10], diabetic kidney disease [11,12], and adenine-induced CKD [13,14]. Of these models, the adenine-induced CKD model has many advantages for use as a preclinical CKD model: it is minimally invasive, cost-effective, does not require surgery or postoperative care, and has a low mortality rate [15]. In the adenine-induced CKD model, atrophy of the extensor digitorum longus, soleus, and thigh muscles [13,15], decreased grip strength [16], and impaired physical function [17] have been reported. However, how skeletal muscle atrophy occurs over time in the adenine-induced CKD rat model, how the expressions of muscle anabolic and catabolic genes are altered, and how mitochondrial activity is changed are not clear. The elucidation of the temporal changes in these factors in adenine-induced CKD rat models, which are useful as CKD model rats, will be of great importance for future studies of the prevention and treatment of muscle atrophy in CKD. Therefore, the purpose of this study is to evaluate changes over time in skeletal muscle atrophy, expressions of skeletal muscle anabolic and catabolic genes, and mitochondrial activity according to skeletal muscle type in an adenine-induced CKD model.

2. Materials and Methods

2.1. Animal Model and Experimental Design

Eight-week-old male Wistar rats (Charles River Laboratories Inc., Tokyo, Japan) were maintained in a controlled environment (temperature: 23 ± 2 °C, humidity: 40 ± 20%) with a 12 h light–dark cycle. The rats had free access to both water and food. Further details can be found in our previous study [18]. The control group was fed a standard rodent chow (CE-7; Clea Japan, Tokyo, Japan) as their regular diet. The CKD group received a 0.75% adenine diet (Oriental Yeast Co., Ltd., Tokyo, Japan) from 8 to 12 weeks of age, followed by a standard rodent chow diet. The 4-week treatment with the adenine diet was based on a previous study [19]. The results of blood biochemistry tests and renal histological findings in the adenine-induced CKD rat model used in this study were reported in our previously published paper [20]. The serum creatinine, phosphorus, and intact-parathyroid hormone (PTH) levels were elevated, and serum calcium levels were normal, indicating stage IV CKD. There was also an enlargement of the urinary cavities and fibrosis of the renal interstitium in the CKD rats at 20 weeks [20]. Both the control and the CKD groups were sacrificed at 12 and 20 weeks of age (n = 7 rats per group), and the following parameters were evaluated. The animal protocols were approved by our institution’s Animal Care and Use Committee (approval number a-1-3070). Furthermore, all subsequent animal experiments were conducted under the Animal Care and Use Guidelines of our institute, which follow the guidelines for animal research prescribed by the National Research Council’s Guide for the Care and Use of Laboratory Animals.

2.2. Tissue Preparation

The back muscles were collected and immediately stored in liquid nitrogen for the measurement of cross-sectional area (CSA) and succinate dehydrogenase (SDH) staining. Right tibialis anterior (TA) muscles were preserved in RNAlater solution (Qiagen, Hilden, Germany) at −80 °C for subsequent real-time polymerase chain reaction (PCR) testing.

2.3. Histological Analysis of Muscle and Kidney

The back muscles in the four groups were analyzed histologically. Samples were cut into 10 µm thick transverse serial sections at the thickest part of the muscle belly, with the cryostat maintained at −18 °C. The sections were subjected to histochemical staining with adenosine triphosphatase (ATP) after preincubation at pH 10.6. When stained at this pH, type I muscle fibers stain lightly, and type II muscle fibers stain darkly. To quantify the CSA of muscle fibers, five randomly selected fields were assessed, with fifty fibers measured per muscle, and the mean CSA for one muscle fiber was calculated, as previously described [18]. In addition, to assess tubulointerstitial disorder, three-micrometer-thick sections were stained with Elastica–Masson stains. The CSA and kidney were assessed using an all-in-one fluorescence microscope (BZ-X800, KEYENCE, Osaka, Japan).

2.4. Mitochondrial Activity

In addition, succinate dehydrogenase (SDH) staining was performed to evaluate mitochondrial activity. The sections were incubated for 30 min with SDH (0.4 g sodium succinate, 0.04 g nitro-blue tetrazolium (NBT), 0.001 mg phenazine methosulfate) in 0.1 M Tris buffer at 37 °C, then extracted with 30–90% acetone and rinsed with distilled H₂O, as previously reported [7]. The staining density was set to be divided into 256 levels (0–255), and the average intensity was calculated using the luminance measurement function of an all-in-one fluorescence microscope (BZ-X800, KEYENCE). For SDH staining intensity of muscle fibers, five randomly selected fields per muscle were evaluated, and the average staining intensity was calculated. Furthermore, the number of SDH-positive fibers in each region was counted and expressed as a percentage by dividing by the total number of fibers in the entire visual field.

2.5. Gene Expression Analysis of Skeletal Muscle

The gene expressions of myogenic determination gene number 1 (MyoD) and myogenin as indicators of muscle anabolism; atrogin-1 and MuRF1 as indicators of muscle catabolism; and peroxisome proliferator-activated receptor-γ coactivator-1-α (PGC-1α) as a marker of mitochondrial biogenesis were evaluated [20]. Tissue disruption was conducted using a homogenizer (MS-100R; Tomy, Tokyo, Japan). Total RNA was isolated from these tissues with a TRIzol reagent (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions. The RNA concentration was measured using a NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA). First-strand complementary DNA (cDNA) synthesis was conducted using a First-Strand cDNA Synthesis Kit (GE Healthcare, Milwaukee, WI, USA). Quantitative reverse-transcription PCR was performed using a Light Cycler 480 system (Roche, West Sussex, United Kingdom) according to the manufacturer’s instructions, with TaqMan probes specific for rat MyoD (TaqMan probe ID: Rn01457527_g1), myogenin (TaqMan probe ID: Rn01490689_g1), atrogin-1 (TaqMan probe ID: Rn00591730_m1), MuRF1 (TaqMan probe ID: Rn00590197_m1), and PGC-1α (TaqMan probe ID: Rn00580241_m1). Glyceraldehyde-3-phosphate dehydrogenase amplification was used as an internal control for sample normalization (TaqMan probe ID: Rn01775763_g1). The cycle number at which the amplification plot intersected with the threshold (CT) was determined, and the ΔΔCT method was used to analyze the relative changes in gene expression.

2.6. Statistical Analyses

All data are presented as mean ± standard deviation (SD) values. Given that the gene expression results were not normally distributed, the nonparametric gene expression data underwent analysis via the Kruskal–Wallis test, with the Steel-Dwass method for post hoc comparisons. The Mann–Whitney U-test was employed to evaluate variations in CSA and SDH staining across different groups. All statistical analyses were conducted using EZR, a graphical user interface for R (The R Foundation for Statistical Computing, Vienna, Austria). It is noteworthy that EZR is an enhanced version of R Commander that has been designed to integrate common biostatistical functions [21]. A p-value of less than 0.05 was considered statistically significant.

3. Results

3.1. Histological Findings and Cross-Sectional Area of Back Muscle

An ATP-stained histological section of back muscle is shown in Figure 1A. In the CKD rats, the muscle fibers of both type I and type II at 12 weeks, and of only type II at 20 weeks, were smaller than in the controls. At 12 weeks, the CSAs of the back muscle fibers of both type I (Figure 1B) and type II (Figure 1C) were significantly smaller in the CKD group than in the control group (p < 0.01). At 20 weeks, the CSA of only type II (Figure 1E) was significantly smaller in the CKD group than in the control group (p < 0.01), but there was no significant difference in type I (Figure 1D).

3.2. Mitochondrial Activity Evaluated with SDH Staining

The SDH-stained histological sections showed greater decreased SDH intensity in the CKD rats than in the control rats at 12 weeks, but not at 20 weeks (Figure 2A). Although SDH staining intensity was significantly lower in the CKD group than in the control group at 12 weeks (p < 0.05) (Figure 2B), there was no significant difference in staining intensity between the control and CKD groups at 20 weeks (Figure 2C). The percentage of SDH-positive fibers was significantly lower in the CKD rats at 12 weeks (Figure 2D). There was no significant difference at 20 weeks (Figure 2E).

3.3. Histological Sections of the Kidney

The histological section of the kidney in the control group shows less fibrosis of the renal interstitium. On the other hand, in the CKD group, the extent of fibrosis is increased at both 12 weeks and 20 weeks (Figure 3).

3.4. Gene Expression of Skeletal Muscle

In the TA muscle, MyoD gene expression levels showed no significant differences between the control and CKD groups at both 12 and 20 weeks (Figure 4A). At 12 weeks, muscle anabolic-related gene expression (myogenin) was significantly more elevated in the CKD group than in the control group, but this difference was not observed at 20 weeks (Figure 4B). The mRNA levels of genes associated with muscle catabolism, atrogin-1 and MuRF-1, were markedly higher in the CKD group at 12 weeks (both p < 0.01), with no significant differences at 20 weeks (Figure 4C,D). The significant increases in myogenin, atrogin-1, and MuRF-1 expression observed in the CKD group at 12 weeks were notably decreased by 20 weeks (all p < 0.01) (Figure 4B–D). The expression of the PGC-1α mRNA indicated a potential increase in the CKD group at 12 weeks, although it did not reach statistical significance between the groups (Figure 4E).

4. Discussion

In the present study, changes over time in skeletal muscle atrophy were examined in a CKD model of male Wistar rats that were fed a 0.75% adenine diet for 4 weeks, starting at 8 weeks of age. At 20 weeks of age, only the type II muscle CSA was decreased. SDH staining, which reflects mitochondrial activity, was lower in the CKD group than in the control group at 12 weeks of age, but there was no significant difference at 20 weeks of age. Regarding the expression of genes related to muscle differentiation, increased expressions of myogenin, a myogenic regulatory factor, and of MuRF-1 and atrogin-1, muscle catabolic markers, were found only at 12 weeks of age, early after the establishment of CKD, but at 20 weeks of age, there were no differences in their expressions.

In the present study, skeletal muscle atrophy in adenine-induced CKD model rats was found to change over time in different types of muscle fibers. Several previous studies involving CKD model rats have reported that skeletal muscle atrophy and changes in the molecular cellular mechanisms associated with muscle atrophy were different in adenine-induced CKD and 5/6 nephrectomy [7,15]. However, no studies have focused on these changes over time. The present study showed differences in the type of skeletal muscle that atrophies at 12 and 20 weeks of age, differences in gene expression, and differences in mitochondrial activity. These differences in gene expression and mitochondrial activity may account for the differences in the characteristics of muscle atrophy.

In the adenine-induced CKD rat model, the CSAs of both type I and type II skeletal muscles were significantly reduced compared to the controls at 12 weeks of age, but only the CSA of type II skeletal muscle was significantly lower at 20 weeks of age, with no significant difference observed in type I skeletal muscle. In general, type I muscle fibers are more susceptible to atrophy caused by inactivity and denervation, whereas type II muscle fibers are more susceptible to aging, diabetes mellitus, chronic heart failure, and cancer [22,23]. It has been reported that fast-twitch muscle fibers tend to be decreased in CKD patients and CKD model rats [24]. Type II muscle fibers have a poorly distributed capillary network, which is thought to be due to the decreased hemoglobin concentration caused by worsening renal function due to CKD, resulting in tissue hypoxia [25]. Furthermore, it has been reported that a fast-to-slow, fiber-type shift is observed with muscle atrophy, such as aging [23]. These changes in muscle atrophy over time may indicate CKD-induced skeletal muscle atrophy, and compensatory improvement in the CSA of slow type I muscle fibers.

In the adenine-induced CKD model rats, the mRNA expression levels of myogenin were significantly higher only at 12 weeks of age. However, the MyoD levels were not significantly different between the groups. MyoD and myogenin are important factors in muscle regeneration [26], and impaired skeletal muscle regeneration is involved in muscle atrophy. When a skeletal muscle is injured, satellite cells initiate proliferation and differentiation, and the expressions of MyoD and myogenin increase [27]. MyoD expression is associated with satellite cell activation and proliferation, whereas myogenin reflects myoblast differentiation; a study examining skeletal muscle from Cy/+ rats reported that both MyoD and myogenin were upregulated [7], a result different from the present study. The increased expression of myogenin observed in the present study may reflect the process of muscle regeneration in an adenine-induced CKD rat model. Myogenin is involved in myoblast differentiation and acts to regulate myofiber maturation and size [28]. Among its functions, it has been suggested that it induces the fusion of skeletal muscle fibers, mainly affecting the formation of fast-twitch muscle fibers and muscle hypertrophy [29]. In the present study, myogenin expression was increased in 12-week-old CKD model rats. This may be a compensatory mechanism for the muscle wasting that occurs in the CKD rat model. In contrast, no significant difference was observed for MyoD, a myogenic marker.

It has been suggested that uremic toxin (UT) is also involved in muscle atrophy and weakness in CKD [30,31]. One in vitro study reported that different doses of uremic substances affect the myogenic process differently. Low doses of UT impair normal myogenic differentiation by promoting fibrotic and adipogenic differentiation of myoblasts, whereas high doses of UT impair myoblasts by inducing cell cycle arrest, disrupting their proliferation, and causing apoptosis [32]. MyoD is expressed at the muscle differentiation stage when satellite cells are activated before myoblast differentiation. Therefore, the difference in the expression of myogenic markers in the present study suggests that the model may have been exposed to high concentrations of uremic substances.

In the adenine-induced CKD rat model, SDH staining intensity was decreased only at 12 weeks. Furthermore, the percentage of SDH-positive fibers was significantly lower only in CKD rats at 12 weeks. This result suggests that mitochondrial activity was reduced early after the model was created. It has been reported that mitochondrial dysfunction is associated with muscle weakness in CKD [33]. It has also been reported that CKD is preceded by a decrease in muscle strength and muscle mass, and is associated with a decrease in muscle mitochondrial function [34]. In addition, type I muscle fibers that showed reduced CSA in this study were rich in mitochondria [35], and the reduction in type I muscle CSA early after model creation may be related to reduced mitochondrial activity.

The present study examined the changes in skeletal muscle atrophy over time in a rat model of adenine-induced CKD. This study has several limitations. First, it was not possible to confirm serum creatinine levels and other data in each group; thus, the relationship between blood data and muscle atrophy could not be evaluated. However, since the method used to create the model was the same as that in our previous study, which confirmed that creatinine levels worsened over time, we assumed that the early and late stages of CKD onset were compared in the present study. Second, nutritional status was not assessed. There is a trend toward weight loss when adenine is administered [36]. In our previous report of adenine-induced CKD rat models generated using the same protocol as in the present study [37], body weight was also decreased, suggesting that adenine administration may have contributed to the weight loss and associated deterioration in nutritional status. Third, the evaluation was conducted on the back muscles and the TA muscles, and a comprehensive evaluation of the entire model is lacking. To evaluate muscle change in this model, it is necessary to consider muscles in other regions, such as the soleus muscle or quadriceps muscle. The investigation of muscle-related gene expression in the soleus muscle, which contains much more type I (slow) muscle fiber, similar to the back muscle, or the evaluation of CSA and mitochondrial activity with SDH-stained sections in the quadriceps muscle, which has much more type II (fast) muscle fiber, could provide a more comprehensive picture of muscle atrophy by CKD. Furthermore, additional observations of ultrastructural changes in muscle fibers by transmission electron microscopy may enhance our comprehension of the alterations in muscle atrophy within this model, in addition to the histological evaluation conducted in this study.

5. Conclusions

The adenine-induced CKD rat model shows that type I and type II muscle CSAs were decreased at 12 weeks of age, early after adenine administration, but type I muscle CSA was recovered at 20 weeks of age. In addition, increased expression of muscle catabolic markers and myogenin, and decreased SDH staining intensity, was observed at 12 weeks. These phenomena may be related to changes in muscle atrophy over time.