Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens
Abstract
:Featured Application
Abstract
1. Introduction
2. Materials and Methods
2.1. Isolation of Ligninolytic and Peroxidase-Producing Bacteria
2.2. Exoperoxidase Production under Submerged Fermentation (SMF)
2.3. Determination of Peroxidase Activity
2.4. Determination of Process Parameters for Optimal Exoperoxidase Production
2.5. Exoperoxidase Production Over a Time Course
2.6. Utilization of Agricultural Residues for Exoperoxidase Production
2.7. Polymerase Chain Reaction (PCR) Detection of Peroxidase Gene
2.8. Bioinformatics Analysis
2.9. Data Analysis
2.10. Accession Number
3. Results and Discussion
3.1. Determination of Process Parameters for Optimal Exoperoxidase Production
3.2. Exoperoxidase Production Over a Time Course
3.3. Assessment of Agricultural Residues for Exoperoxidase Production
3.4. PCR Detection of Peroxidase Gene
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AL | alkali lignin |
ALM | alkali lignin medium |
ANOVA | analysis of variance |
BLAST | basic local alignment search tool |
FA | ferulic acid |
GA | guaiacol |
LMEs | lignin modifying enzymes |
MEGA | molecular evolutionary genetics analysis |
NAD | not detected |
NCBI | National Center for Biotechnology Information |
OD | optical density |
SMF | submerged fermentation |
SSF | solid state fermentation |
UV | ultraviolet |
VALC | veratryl alcohol |
VAN | vanillin |
VA | vanillic acid |
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Agricultural Residue | Protein Concentration (mg mL−1) | Enzyme Assay (With H2O2) | Enzyme Assay (Without H2O2) | ||
---|---|---|---|---|---|
Peroxidase Production (U mL−1) | Specific Productivity (U mg−1) | Probable Laccase Production (U mL−1) | Specific Productivity (U mg−1) | ||
Sawdust | 0.028 ± 0.0 a | 1.05 ± 0.00 a | 37.50 ± 0.00 a | NAD * | NAD * |
Wheat Straw | 1.023 ± 0.116 b | 5.37 ± 0.00 b | 5.25 ± 0.00 b | NAD * | NAD * |
Corn Stover | 1.366 ± 0.021 b | 5.13 ± 0.00 b | 3.76 ± 0.00 c | 1.93 ± 0.53 | 1.23 ± 0.21 |
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Falade, A.; Jaouani, A.; Mabinya, L.; Okoh, A.; Nwodo, U. Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens. Appl. Sci. 2019, 9, 3121. https://doi.org/10.3390/app9153121
Falade A, Jaouani A, Mabinya L, Okoh A, Nwodo U. Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens. Applied Sciences. 2019; 9(15):3121. https://doi.org/10.3390/app9153121
Chicago/Turabian StyleFalade, Ayodeji, Atef Jaouani, Leonard Mabinya, Anthony Okoh, and Uchechukwu Nwodo. 2019. "Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens" Applied Sciences 9, no. 15: 3121. https://doi.org/10.3390/app9153121