Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Gold Nanoparticles (AuNPs) Synthesis
2.2. Characterization of AuNP
2.3. BSA-FITC Conjugation
2.4. Colloidal Stability in Biological Media
2.5. Protein Corona Formation and Quantification
3. Results and Discussion
3.1. Gold Nanoparticle Synthesis and Characterization
3.2. Stability Studies
3.3. Protein Corona Quantification
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Nanoparticle | Ligand | λspr (nm) | Hydrodynamic Diameter (nm) | Zeta Potential (mV) |
---|---|---|---|---|
AuNP 24 nm | citrate | 523.5 | 30.4 | −34.3 |
PEG-COOH 5 kDa | 525 | 31.2 | −30.4 | |
PEG-COOH 20 kDa | 524.5 | 30.9 | −32.3 | |
PEG-OCH3 | 525 | 32.3 | −2.7 | |
PEG-NH2 | 526 | 35.8 | 28.2 | |
AuNP 56 nm | citrate | 534 | 64.2 | −32.4 |
PEG-COOH 5 kDa | 537 | 66.3 | −30.4 | |
PEG-COOH 20 kDa | 536.5 | 66.7 | −31.2 | |
PEG-OCH3 | 537 | 65.8 | 1.3 | |
PEG-NH2 | 539 | 69.2 | 30.2 |
Nanoparticle | PEG Ligand | % Recovery | ||
---|---|---|---|---|
1 ng | 50 ng | 100 ng | ||
AuNP 24 nm | PEG-COOH 5 kDa | 98.1 | 98.1 | 101.1 |
PEG-COOH 20 kDa | 98.2 | 98.2 | 99.2 | |
PEG-OCH3 | 99.5 | 99.5 | 98.5 | |
PEG-NH2 | 97.1 | 97.1 | 98.4 | |
AuNP 56 nm | PEG-COOH 5 kDa | 97.2 | 99.7 | 98.2 |
PEG-COOH 20 kDa | 98.4 | 102.4 | 99.4 | |
PEG-OCH3 | 99.7 | 99.8 | 98.2 | |
PEG-NH2 | 96.4 | 98.6 | 100.3 |
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Nicoară, R.; Ilieș, M.; Uifălean, A.; Iuga, C.A.; Loghin, F. Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry. Appl. Sci. 2019, 9, 4789. https://doi.org/10.3390/app9224789
Nicoară R, Ilieș M, Uifălean A, Iuga CA, Loghin F. Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry. Applied Sciences. 2019; 9(22):4789. https://doi.org/10.3390/app9224789
Chicago/Turabian StyleNicoară, Raul, Maria Ilieș, Alina Uifălean, Cristina Adela Iuga, and Felicia Loghin. 2019. "Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry" Applied Sciences 9, no. 22: 4789. https://doi.org/10.3390/app9224789