Effects of Coriandrum sativum L. in Association with Physical Exercise in Alloxan-Induced Type 1 Diabetes Mellitus in Rats

Round 1

Reviewer 1 Report

This is an interesting manuscript which requires further refinement to the way data is presented.

Please provide hypothesis in your abstract and the introduction. Further information is required how ( mechanism if known ) coriander might improve type 1 diabetes. Justification for the chosen tests is required.

If the data for non diabetic rats is available this should be provided as control non-diabetic rats 

Author Response

Response to Reviewer 1 Comments

 

Point 1: Please provide hypothesis in your abstract and the introduction. Further information is required how (mechanism if known) coriander might improve type 1 diabetes. Justification for the chosen tests is required.

 

Response 1: Dear reviewer, we follow your consideration and in a new version we elucidate this question about how coriander might improve the type 1 diabetes.

 

Point 2: If the data for non diabetic rats is available this should be provided as control non-diabetic rats 

 

Response 2: We previously published a study where evaluating the same parameters used on this work, although, in healthy animals. We refer this work in the present investigation to show that the previous outcomes have evidenced the expect effects about the concomitant treatment.

Author Response File: Author Response.docx

Reviewer 2 Report

The study of Cardoso et al. evaluates the effect of concomitant treatment with coriander extract and whole body vibration exercise on several physiological parameters in Wistar rats with type 1 diabetes mellitus induced with alloxan.

The aim of the present study is very interesting, due to the lack of information on effects of an hypoglycemic medicinal plant such as coriander and low-intensity physical exercise on T1DM.

In my opinion, the general text could be improved and the work should be revised to address different points.

- The authors have to explain the selected coriander concentration (250 mg/ml) administrated to rats. Furthermore, in section 2.4 of experimental procedures, the authors referred to their previous work (ref. 24) in which they used 8 mg/ml of coriander instead of 250 mg/ml. Could you explain the reason of this difference?
- Also in the discussion the authors referred to their previous work (ref. 24) stating the use of "identical experimental design". It might be true for all the parameters evaluated, but the concentration of the tested compound seems to be different, and this is not a small issue in my opinion. 
- Considering that it is a concentration administrated, it would be better to convert the dose in mg/kg
- In the discussion the authors stated that the used DM model, obtained trough the alloxan drug, could explain the unexpected results. The authors need to elucidate better this hypothesis, also explaining the choice of alloxan instead of streptozotocin.
- Lack of the proper reference "Das S, Chaware S, Narkar N, Tilak AV, Raveendran S, Rane P. Antidiabetic activity of Coriandrum sativum in streptozotocin induced diabetic rats. Int J Basic Clin Pharmacol 2019;8:925-9" maybe cited in the discussion section

 

 

Author Response

Response to Reviewer 2 Comments

 

Point 1: The authors have to explain the selected coriander concentration (250 mg/ml) administrated to rats. Furthermore, in section 2.4 of experimental procedures, the authors referred to their previous work (ref. 24) in which they used 8 mg/ml of coriander instead of 250 mg/ml. Could you explain the reason of this difference?

 

Response 1: Dear reviewer, we made a mistake about the concentration described. In fact, the concentration used on the experiment was 8 mg/ml. The value of 250 mg/ml belongs to another work that we are drafting. The present manuscript is a continuation of a doctoral project, which we have published evaluating the same parameters used on this work, although, in healthy animals [ref 24] in the sent version. We, authors, sincerely apologize for this fact.

 

Point 2: Also, in the discussion the authors referred to their previous work (ref. 24) stating the use of "identical experimental design". It might be true for all the parameters evaluated, but the concentration of the tested compound seems to be different, and this is not a small issue in my opinion.

 

Response 2: As answered on Point 1, the parameters of the present study are the same used on the previous work (ref 24).

 

Point 3: In the discussion the authors stated that the used DM model, obtained through the alloxan drug, could explain the unexpected results. The authors need to elucidate better this hypothesis, also explaining the choice of alloxan instead of streptozotocin. Lack of the proper reference "Das S, Chaware S, Narkar N, Tilak AV, Raveendran S, Rane P. Antidiabetic activity of Coriandrum sativum in streptozotocin induced diabetic rats. Int J Basic Clin Pharmacol 2019;8:925-9" maybe cited in the discussion section.

 

Response 3: We follow your consideration and we elucidate this question about the why use Alloxan. In fact, Alloxan s a diabetogenic agent, used to model the type 1 diabetes (T1DM) on in vivo studies due to a severe insulin deficiency produced, as well as marked hyperglycemic, ketoacidotic and glycosuric in experimental models. This was one of the reasons why we decided for this drug, the second was for the lack of information on new therapeutic solutions for treat T1DM.

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

Thank you for amending this manuscript. This is a negative study and therefore is not making a great discovery. In future using historical control group would not be excepted. The data should  always be analysed in parallel.

Please correct English Grammar