Comprehensive Analysis of Age- and Sex-Related Expression of the Chaperone Protein Sigma-1R in the Mouse Brain
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Antibodies and Reagents
2.3. Plasmid Constructs
2.4. Cell Culture and siRNA Assay
2.5. Tissue Homogenization
2.6. Western Blotting (WB)
2.7. Densitometry Analysis
2.8. Perfusion and Cryo-Sectioning
2.9. Immunohistochemistry (IHC) & Antibody Validation
2.10. Confocal Imaging and Image Analyses
2.11. Statistical Analysis
3. Results
3.1. S1R Is Differentially Expressed in Males and Females and Reduces during Aging in the Hippocampus, Cortex, and Striatum
3.2. Histological Analysis of S1R in Brain Tissue and Antibody Validation
3.3. General Observation of S1R Distribution in the Mouse Brain
3.4. S1R Expression and Distribution in the Hippocampus, Amygdala, Hypothalamus, and Thalamus
3.4.1. The Hippocampus
3.4.2. The Amygdala
3.4.3. The Hypothalamus
3.4.4. The Thalamus
3.5. S1R Expression and Distribution in the Cortex and Striatum
3.5.1. The Motor and Cingulate Cortex
3.5.2. The Striatum
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Brain Regions | Female | Male | ||||
---|---|---|---|---|---|---|
Juvenile | Early Adult | Middle-Aged | Juvenile | Early Adult | Middle-Aged | |
Hippocampus | ||||||
CA1 | 73.56 ± 7.13 | 51.59 ± 6.61 | 39.61 ± 7.25 | 100 | 43.72 ± 8.75 | 38.94 ± 5.87 |
CA3 | 103.07 ± 2.74 | 70.91 ± 9.5 | 56.87 ± 3.58 | 98.19 ± 10.02 | 83.83 ± 3.26 | 45.64 ± 6.03 |
DG | 84.91 ± 10.74 | 32.09 ± 2.10 | 31.83 ± 2.93 | 94.54 ± 18.97 | 34.55 ± 10.75 | 39.27 ± 8.02 |
Thalamus | 6.86 ±0.73 | 4.58 ± 0.19 | 2.15 ± 0.25 | 7.22 ± 0.44 | 4.30 ± 0.16 | 2.06 ± 0.11 |
Amygdala | 55.02 ± 13.97 | 21.60 ± 2.56 | 15.34 ± 1.76 | 54.95 ± 14.87 | 21.46 ± 8.29 | 16.83 ± 1.06 |
Hypothalamus | 21.52 ± 4.20 | 6.42 ± 1.05 | 6.92 ± 1.28 | 13.33 ± 0.41 | 7.15 ± 1.81 | 7.17 ± 1.06 |
Cortex | ||||||
Cingulate | 21.61 ± 2.05 | 10.94 ± 2.09 | 5.94 ± 1.84 | 24.42 ± 2.74 | 8.96 ± 1.04 | 4.87 ± 0.35 |
Motor | 22.95 ± 4.84 | 8.40 ± 0.6 | 4.98 ± 0.49 | 20.42 ± 3.83 | 6.95 ± 0.81 | 3.66 ± 0.57 |
Striatum | ||||||
Dorsal | 18.01 ± 1.33 | 9.74 ± 0.86 | 7.78 ± 0.31 | 20.38 ± 5.12 | 10.65 ± 0.84 | 6.69 ± 0.90 |
NAc Shell | 30.22 ± 2.20 | 22.66 ± 2.83 | 18.98 ± 1.00 | 31.27 ± 4.05 | 19.22 ± 1.76 | 17.59 ± 3.89 |
NAc Core | 28.21 ± 2.65 | 20.94 ± 3.11 | 19.05 ± 2.75 | 35.55 ± 2.98 | 25.75 ± 2.97 | 16.80 ± 2.78 |
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Tarmoun, K.; Lachance, V.; Le Corvec, V.; Bélanger, S.-M.; Beaucaire, G.; Kourrich, S. Comprehensive Analysis of Age- and Sex-Related Expression of the Chaperone Protein Sigma-1R in the Mouse Brain. Brain Sci. 2024, 14, 881. https://doi.org/10.3390/brainsci14090881
Tarmoun K, Lachance V, Le Corvec V, Bélanger S-M, Beaucaire G, Kourrich S. Comprehensive Analysis of Age- and Sex-Related Expression of the Chaperone Protein Sigma-1R in the Mouse Brain. Brain Sciences. 2024; 14(9):881. https://doi.org/10.3390/brainsci14090881
Chicago/Turabian StyleTarmoun, Khadija, Véronik Lachance, Victoria Le Corvec, Sara-Maude Bélanger, Guillaume Beaucaire, and Saïd Kourrich. 2024. "Comprehensive Analysis of Age- and Sex-Related Expression of the Chaperone Protein Sigma-1R in the Mouse Brain" Brain Sciences 14, no. 9: 881. https://doi.org/10.3390/brainsci14090881