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Antioxidants
Volume 12
Issue 7
10.3390/antiox12071329
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Article

The Cactus (Opuntia ficus-indica) Cladodes and Callus Extracts: A Study Combined with LC-MS Metabolic Profiling, In-Silico, and In-Vitro Analyses

by
Dong-Geon Nam
1,
Hee-Sun Yang
1,
Ui-Jin Bae
1,
Eunmi Park
2,
Ae-Jin Choi
1 and
Jeong-Sook Choe
1,*
1
Division of Functional Food & Nutrition, Department of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun 55365, Republic of Korea
2
Department of Food and Nutrition, Hannam University, Daejeon 306-791, Republic of Korea
*
Author to whom correspondence should be addressed.
Antioxidants 2023, 12(7), 1329; https://doi.org/10.3390/antiox12071329
Submission received: 9 May 2023 / Revised: 17 June 2023 / Accepted: 20 June 2023 / Published: 23 June 2023
(This article belongs to the Topic Biological Activity of Plant Extracts)
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Abstract

Opuntia ficus-indica (OF) phytochemicals have received considerable attention because of their health benefits. However, the structure-activity relationship between saponin and flavonoid antioxidant compounds among secondary metabolites has rarely been reported. In a molecular docking study, selected compounds from both Opuntia ficus-indica callus (OFC) and OF ethanol extract were found to be involved in Toll-like receptor 4 and mitogen-activated protein kinase (MAPK) signaling pathways. High affinity was specific for MAPK, and it was proposed to inhibit the oxidative and inflammatory responses with poricoic acid H (−8.3 Kcal/mol) and rutin (−9.0 Kcal/mol). The pro-inflammatory cytokine factors at a concentration of 200 μg/mL were LPS-stimulated TNF-α (OFC 72.33 ng/mL, OF 66.78 ng/mL) and IL-1β (OFC 49.10 pg/mL, OF 34.45 pg/mL), both of which significantly decreased OF (p < 0.01, p < 0.001). Taken together, increased NO, PGE2, and pro-inflammatory cytokines were significantly decreased in a dose-dependent manner in cells pretreated with OFC and the OF extract (p < 0.05). These findings suggest that OFC and OF have important potential as natural antioxidant, anti-inflammatory agents in health-promoting foods and medicine.
Keywords: Opuntia ficus-indica cladodes; callus; ethanolic extract; phytochemical; LC-MS; molecular docking; antioxidant; anti-inflammatory Opuntia ficus-indica cladodes; callus; ethanolic extract; phytochemical; LC-MS; molecular docking; antioxidant; anti-inflammatory
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MDPI and ACS Style

Nam, D.-G.; Yang, H.-S.; Bae, U.-J.; Park, E.; Choi, A.-J.; Choe, J.-S. The Cactus (Opuntia ficus-indica) Cladodes and Callus Extracts: A Study Combined with LC-MS Metabolic Profiling, In-Silico, and In-Vitro Analyses. Antioxidants 2023, 12, 1329. https://doi.org/10.3390/antiox12071329

AMA Style

Nam D-G, Yang H-S, Bae U-J, Park E, Choi A-J, Choe J-S. The Cactus (Opuntia ficus-indica) Cladodes and Callus Extracts: A Study Combined with LC-MS Metabolic Profiling, In-Silico, and In-Vitro Analyses. Antioxidants. 2023; 12(7):1329. https://doi.org/10.3390/antiox12071329

Chicago/Turabian Style

Nam, Dong-Geon, Hee-Sun Yang, Ui-Jin Bae, Eunmi Park, Ae-Jin Choi, and Jeong-Sook Choe. 2023. "The Cactus (Opuntia ficus-indica) Cladodes and Callus Extracts: A Study Combined with LC-MS Metabolic Profiling, In-Silico, and In-Vitro Analyses" Antioxidants 12, no. 7: 1329. https://doi.org/10.3390/antiox12071329

APA Style

Nam, D.-G., Yang, H.-S., Bae, U.-J., Park, E., Choi, A.-J., & Choe, J.-S. (2023). The Cactus (Opuntia ficus-indica) Cladodes and Callus Extracts: A Study Combined with LC-MS Metabolic Profiling, In-Silico, and In-Vitro Analyses. Antioxidants, 12(7), 1329. https://doi.org/10.3390/antiox12071329

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