Effects of Hyperbaric (Non-Thermal) Sanitization and the Method of Extracting Pomegranate Juice on Its Antioxidant and Antihypertensive Properties
Abstract
:1. Introduction
2. Materials and Methods
2.1. Extraction Process
2.1.1. Extraction of Aril Juice Using a Manual Method
2.1.2. Cold Pressing Extraction of Juice from Arils
2.2. Physicochemical Characterization
2.2.1. Bromatological Analysis
2.2.2. pH
2.2.3. Color Determination
2.2.4. Fourier Transform Infrared Spectroscopy (FT-IR)
2.2.5. Microbiological Analysis
2.2.6. Total Phenols Determination
2.2.7. Flavonoids Determination
2.2.8. Determination of Total Monomeric Anthocyanin Content Using the pH Differential Method for Total Anthocyanins (AT)
- Mw (molecular weight) = 449.2 g/mol for cyanidin-3-glucoside (cyd-3-glu).
- DF = dilution factor established in D.
- 1 = pathlength in cm.
- ε = 26,900 molar extinction coefficient, in L mol−1 cm−1, for cyd-3-glu.
- The factor for conversion from g into mg was 1000.
- A = absorbance.
- Mw = molecular weight.
- DF = dilution factor.
2.2.9. Determination of ABTS Radical Scavenging
2.2.10. Determination of DPPH Radical Scavenging
2.3. Inhibition of Angiotensin Converting Enzyme (ACE) Activity by Pomegranate Juice
- StdSlope = slope of the standard curve subtracted from the blank (RFU/nmol);
- DF = dilution factor (if the sample was not diluted, the DF value was 1);
- SlopeSample = slope of the sample curve subtracted from the blank (RFU/min).
2.4. Statistical Analysis
3. Results and Discussions
3.1. Bromatological Analysis
3.2. pH Value
3.3. Color Determination
3.4. Fourier Transform Infrared Spectroscopy (FT-IR)
3.5. Microbiological Analysis
3.6. Phenols, Flavonoids, and Anthocyanins Content in Juice from Arils
3.6.1. Phenols
3.6.2. Flavonoids
3.6.3. Anthocyanins
3.7. Determination of Scavenging of ABTS and DPPH Radicals
3.8. Antihypertensive Analysis
Analysis In Vivo
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Samples | Moisture (%) | Total Solids (%) | Fat (%) | Ash (%) | Protein (%) | Carbohydrates (%) |
---|---|---|---|---|---|---|
Man-P | ||||||
Mech-Hyp |
Samples | L | a* | b* |
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Man-P | |||
Mech-Hyp |
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Campos-Lozada, G.; Hernández-Miranda, J.; del Valle-Mondragón, L.; Ortiz-Polo, A.; Betanzos-Cabrera, G.; Aguirre-Álvarez, G. Effects of Hyperbaric (Non-Thermal) Sanitization and the Method of Extracting Pomegranate Juice on Its Antioxidant and Antihypertensive Properties. Antioxidants 2024, 13, 1009. https://doi.org/10.3390/antiox13081009
Campos-Lozada G, Hernández-Miranda J, del Valle-Mondragón L, Ortiz-Polo A, Betanzos-Cabrera G, Aguirre-Álvarez G. Effects of Hyperbaric (Non-Thermal) Sanitization and the Method of Extracting Pomegranate Juice on Its Antioxidant and Antihypertensive Properties. Antioxidants. 2024; 13(8):1009. https://doi.org/10.3390/antiox13081009
Chicago/Turabian StyleCampos-Lozada, Gieraldin, Jonathan Hernández-Miranda, Leonardo del Valle-Mondragón, Araceli Ortiz-Polo, Gabriel Betanzos-Cabrera, and Gabriel Aguirre-Álvarez. 2024. "Effects of Hyperbaric (Non-Thermal) Sanitization and the Method of Extracting Pomegranate Juice on Its Antioxidant and Antihypertensive Properties" Antioxidants 13, no. 8: 1009. https://doi.org/10.3390/antiox13081009