Involvement of KV3.4 Channel in Parkinson’s Disease: A Key Player in the Control of Midbrain and Striatum Differential Vulnerability during Disease Progression?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Primary Astrocytes from A53T-α-Syn Mice
2.3. Western Blot Analysis
2.4. Apoptosis-Related Protein Analysis
2.5. Intracellular ROS Release Measurement by Cytofluorimetry
2.6. Electrophysiology
2.7. Ca2+i Measurement
2.8. Data and Statistical Analysis
3. Results
3.1. Time Dependent Correlation among KV3.4 Channel and p27kip1, XIAP, and Smac/DIABLO in A53T Midbrain and Striatum
3.2. Different Time-Dependent Proteome Profiler Displayed by A53T Midbrain and Striatum
3.3. Different Modulation of ROS-Dependent KV3.4 Channel in Primary A53T Midbrain and Striatum Astrocytes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Magliocca, G.; Esposito, E.; Tufano, M.; Piccialli, I.; Rubino, V.; Tedeschi, V.; Sisalli, M.J.; Carriero, F.; Ruggiero, G.; Secondo, A.; et al. Involvement of KV3.4 Channel in Parkinson’s Disease: A Key Player in the Control of Midbrain and Striatum Differential Vulnerability during Disease Progression? Antioxidants 2024, 13, 999. https://doi.org/10.3390/antiox13080999
Magliocca G, Esposito E, Tufano M, Piccialli I, Rubino V, Tedeschi V, Sisalli MJ, Carriero F, Ruggiero G, Secondo A, et al. Involvement of KV3.4 Channel in Parkinson’s Disease: A Key Player in the Control of Midbrain and Striatum Differential Vulnerability during Disease Progression? Antioxidants. 2024; 13(8):999. https://doi.org/10.3390/antiox13080999
Chicago/Turabian StyleMagliocca, Giorgia, Emilia Esposito, Michele Tufano, Ilaria Piccialli, Valentina Rubino, Valentina Tedeschi, Maria Jose Sisalli, Flavia Carriero, Giuseppina Ruggiero, Agnese Secondo, and et al. 2024. "Involvement of KV3.4 Channel in Parkinson’s Disease: A Key Player in the Control of Midbrain and Striatum Differential Vulnerability during Disease Progression?" Antioxidants 13, no. 8: 999. https://doi.org/10.3390/antiox13080999