Next Article in Journal
Blood Antibody Titers and Adverse Reactions after BNT162b2 mRNA Vaccination
Next Article in Special Issue
A Diagnostic Strategy for Gauging Individual Humoral Ex Vivo Immune Responsiveness Following COVID-19 Vaccination
Previous Article in Journal
Immunoinformatics and Biophysics Approaches to Design a Novel Multi-Epitopes Vaccine Design against Staphylococcus auricularis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Vaccines
Volume 10
Issue 5
10.3390/vaccines10050639
vaccines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Dynamics of Neutralizing Antibody and T-Cell Responses to SARS-CoV-2 and Variants of Concern after Primary Immunization with CoronaVac and Booster with BNT162b2 or ChAdOx1 in Health Care Workers

by
Watsamon Jantarabenjakul
1,2,3,
Pimpayao Sodsai
4,
Napaporn Chantasrisawad
1,2,3,
Anusara Jitsatja
1,
Sasiprapa Ninwattana
1,
Nattakarn Thippamom
1,
Vichaya Ruenjaiman
4,
Chee Wah Tan
5,
Rakchanok Pradit
1,
Jiratchaya Sophonphan
6,
Supaporn Wacharapluesadee
1,
Lin-Fa Wang
5,
Thanyawee Puthanakit
2,3,†,
Nattiya Hirankarn
4,† and
Opass Putcharoen
1,6,7,*,†
1
Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand
2
Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
3
Center of Excellence in Pediatric Infectious Diseases and Vaccine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
4
Center of Excellence in Immunology and Immune-Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
5
Programme in Emerging Infectious Disease, Duke-NUS Medical School, Singapore 169857, Singapore
6
The HIV Netherlands Australia Thailand Research Collaboration (HIV-NAT), Thai Red Cross AIDS Research Centre, Bangkok 10330, Thailand
7
Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Vaccines 2022, 10(5), 639; https://doi.org/10.3390/vaccines10050639
Submission received: 27 March 2022 / Revised: 14 April 2022 / Accepted: 16 April 2022 / Published: 19 April 2022
(This article belongs to the Special Issue Antibody and Humoral Immunity Research on SARS-CoV-2)
Browse Figures
Review Reports Versions Notes

Abstract

:
Inactivated SARS-CoV-2 vaccine (CoronaVac) is commonly used in national immunization programs. However, the immune response significantly declines within a few months. Our study assessed the immune response against SARS-CoV-2 after receiving booster shots of BNT162b2 or ChAdOx1 among health care workers who previously received CoronaVac as their primary immunization. Fifty-six participants who received ChAdOx1 and forty-two participants who received BNT162b2 were enrolled into this study, which evaluated immune responses, including anti-SARS-CoV-2 spike total antibodies (Elecsys®), surrogated viral neutralization test (sVNT) to ancestral strain (cPass™; GenScript), five variants of concern (Alpha, Beta, Gamma, Delta, and Omicron) (Luminex; multiplex sVNT) and the ELISpot with spike (S1 and S2) peptide pool against the ancestral SARS-CoV-2 strain. The samples were analyzed at baseline, 4, and 12 weeks after primary immunization, as well as 4 and 12 weeks after receiving the booster. This study showed a significant increase in anti-SARS-CoV-2 spike total antibodies, sVNT, and T-cell immune response after the booster, including against the Omicron variant. Immune responses rapidly decreased in the booster group at 12 weeks after booster but were still higher than post-primary vaccination. A fourth dose or a second booster should be recommended, particularly in health care workers.

1. Introduction

In 2020, there are many coronavirus diseases 2019 (COVID-19) vaccines that have been used to prevent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and decrease the severity of the diseases. CoronaVac (an inactivated SARS-CoV-2 vaccine, Sinovac Life Science) is widely used as primary vaccination. A waning immunity of the primary vaccination (two doses of CoronaVac) over time is observed after 12 weeks, which indicates that a booster dose is needed [1]. Moreover, the emergence of variants of concern (VOCs) raises concerns about the vaccine’s effectiveness, which depending on the VOC. The five major VOCs recognized by the World Health Organization (WHO) are Alpha, Beta, Delta, Gamma, and Omicron [2]. In several studies, vaccine efficacy was found to be lower against Delta and Omicron variants in particular [3,4,5].
Homologous or heterologous COVID-19 prime-boost vaccinations are introduced and reported to improve the humoral and cellular immune responses [6,7]. A homologous third dose of CoronaVac demonstrated increased immune responses against SARS-CoV-2; however, immunogenicity was lower when compared to a heterologous prime-boost with BNT162b2 [8,9,10,11,12]. Heterologous prime-boost vaccination with an mRNA vaccine or a viral vector vaccine is widely used as a booster dose because it can induce a high immune response and higher antibody levels, which are likely to provide greater protection against infection, as well as to overcome the new VOCs when there is a lack of specific VOC COVID-19 vaccines [11,13]. However, data on the dynamic immune response to each VOC after the ChAdOx1 booster or BNT162b2 booster in a CoronaVac-based regimen are limited.
The neutralizing antibody (Nab) titer against SARS-CoV-2 is a highly predictive indicator of protective immunity after vaccination or infection, and several of surrogate virus neutralization tests (sVNTs) are widely used. The neutralizing antibody levels from sVNTs are well correlated to the conventional live virus-neutralizing test or pseudovirus-mediated viral neutralization test [14]. However, sVNT needs to have a specific target to the viral spike protein receptor-binding domain (RBD) of each VOC; therefore, a multiplex sVNT platform was developed that was reported to be highly correlated with the live virus-neutralizing test. Additionally, the binding antibody against the SARS-CoV-2 can be used to monitor immune response.
In this study, we describe the dynamics of the immune responses (both antibody and T-cell) against SARS-CoV-2 ancestral type and VOCs in healthy adults who had received a primary immunization with inactivated SARS-CoV-2 (CoronaVac) and a booster with either ChAdOx1 or BNT162b2 at 4 and 12 weeks after receiving the booster.

2. Materials and Methods

2.1. Study Design and Participants

A prospective cohort study was conducted among health care workers (HCWs) aged 18 years or older who received CoronaVac (3–4 weeks apart) as their primary vaccination (2 doses of CoronaVac) at a tertiary care center in Bangkok, Thailand, during March-April 2021 and have results of their immune responses at 4 and 12 weeks after primary vaccination [1]. At 3 months after primary vaccination, the participants were non-randomized to voluntarily receive their booster dose of either ChAdOx1 or BNT162b2.
The exclusion criteria at enrollment were ongoing immunosuppressive medication, any vaccination within 1 month, and having received any blood components or intravenous immunoglobulin within 3 months. The termination criteria were either SARS-CoV-2 infection or having received prophylaxis or investigational treatment against COVID-19.

2.2. Study Procedures

2.2.1. Demographics and Clinical Data

Baseline demographics and clinical data, such as medical history, current medications used, history of exposure to COVID-19 patients, and history of SARS-CoV-2 infection were collected. All HCWs were highly aware of their infection risk. Moreover, they had to follow the surveillance protocol, which included completing a daily questionnaire to screen for COVID-19 symptoms and exposure risk. Any HCWs who had any symptoms or risk factors were tested for SARS-CoV-2 infection using the RT-PCR technique.

2.2.2. Sample Collection and Study Protocol

For antibody response assessment, 4 mL of clotted blood was collected at 4 (+/−1 week) and 12 weeks (+/−2 weeks) after primary vaccination, as well as 4 (+/−1 week) and 12 weeks (+/−2 weeks) after the booster dose with either ChAdOx1 or BNT162b2. For T-cell response assessment, peripheral blood mononuclear cells (PBMCs) from whole blood were isolated by density gradient centrifugation using Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) at 1500 rpm for 30 min at room temperature. Isolated PBMCs were washed with RPMI 1640 medium (Gibco, Life Technologies Corporation, NY, USA), which was supplemented with 10% fetal bovine serum (FBS). The cells were then cryopreserved in 10% dimethyl sulfoxide (DMSO) in FBS for further experiments.

2.2.3. Assessment of Antibody Responses against SARS-CoV-2

The antibody titers against SARS-CoV-2 were determined by anti-SARS-CoV-2 spike total antibodies using the ELISA technique (Elecsys®), surrogate neutralizing antibody to ancestral type (cPassTM, GenScript), and Multiplex sVNT (Luminex) against five VOCs (Alpha, Delta, Beta, Gamma, and Omicron).
  • Anti-SARS-CoV-2 spike total antibodies
Anti-SARS-CoV-2 spike total antibodies were detected by Elecsys® Anti-SARS-CoV-2 S using Cobas e411 immunoassay analyzers (Roche Diagnostics, Rotkreuz, Switzerland), which is also used by the US Food and Drug Administration (FDA) under the Emergency Use Authorization (EUA) Act. Elecsys® (Roche Diagnostics, Rotkreuz, Switzerland) is the immunoassay used to detect total SARS-CoV-2 antibodies against the RBD of the S antigen, and the antibody level is reported as U/mL. This assay has a measuring range of 0.40–250 U/mL. A volume of 200 mL of 100-fold diluted sample was used following the manufacturer’s protocol (measuring range, 40–25,000 U/mL). For the participants who had anti-SARS-CoV-2 spike total antibodies over the maximum measuring range (>25,000 U/mL), the samples were further diluted to 1000-fold and re-evaluated using an Elecsys® Diluent universal kit [15]. The assigned U/mL is equivalent to binding antibody units (BAU/mL), as defined by the WHO International Standard for anti-SARS-CoV-2 immunoglobulin (NIBSC code 20/136). Results reported in U/mL do not need to be converted to another unit and can be directly compared to the results of other studies using BAU/mL [16].
2.
SARS-CoV-2 neutralizing antibodies to ancestral type
The cPass™ SARS-CoV-2 neutralization antibody detection kit (GenScript, Piscataway, NJ, USA) measures SARS-CoV-2-neutralizing antibodies that block recombinant SARS-CoV-2 RBD conjugated with horseradish peroxidase (HRP-RBD) from binding to the human ACE2 receptor protein (hACE2). The neutralizing antibody level was detected according to the manufacturer’s instructions. Serum samples and controls were diluted to 1:10 and pre-incubated with HRP-RBD for 30 min at 37 °C, allowing SARS-CoV-2-neutralizing antibodies to bind to HRP-RBD. The mixture was then added to a microplate pre-coated with hACE2, where the unbound HRP-RBD and HRP-RBD-bound non-neutralizing antibodies were captured. HRP-RBD complexed with neutralizing antibodies was subsequently removed during the washing steps. After washing, 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added, followed by the stop solution to terminate the reaction, and the optical density (OD) of the reaction was measured at 450 nm. The antibody level was reported as the percentage inhibition by using the equation from the manufacturer with a cut-off level for SARS-CoV-2-neutralizing antibody detection of ≥30% inhibition. The seroconversion rate was defined as sVNT ≥ 68%, which was adopted from the US FDA’s guidelines for a high titer of COVID-19 convalescent plasma [17].
3.
Multiplex sVNT to ancestral type SARS-CoV-2 and VOCs
The sVNT was adapted using the Luminex platform to perform multiplex sVNT as previously described [18]. His-Avi-Tag, biotin-labeled receptor-binding domain (RBD) from SARS-CoV-2 ancestral strain and 5 VOCs (Alpha, Delta, Beta, Gamma, and Omicron) were coupled to paramagnetic MagPlex-Avidin microspheres from Luminex. Diluted RBD-coated microsphere mixture containing approximately 600 beads per RBD protein was incubated with 1:10 diluted sera in a 1:1 ratio in a 96-well plate for 1 h at 37 °C, under shaking at 800 rpm. Human ACE2 receptor protein phycoerythrin (PE)-conjugated (1 ug/mL, GenScript) was then added to the plate and incubated for 30 min at 37 °C with shaking at 800 rpm. After incubation, the mixture was washed twice with assay buffer (PBS + 1% bovine serum albumin), followed by resuspension in assay buffer. The plate was then read using a Bioplex MAGPIX system (Luminex, Austin, TX, USA) to acquire the data.

2.2.4. Assessment of T-Cell Response against SARS-CoV-2

SARS-CoV-2-specific T-cell responses were investigated by enzyme-linked immunospot assay (ELISpot assay). ELISpot plates (Millipore) were coated with human IFNγ antibody (1-D1K, Mabtech; 5 μg/mL) overnight at 4 °C. Then, 2.5 × 105 PBMCs were stimulated with overlapping spike (S1 and S2) peptide pool (Genscript, Piscataway NJ, USA) of SARS-CoV-2 in AIM-V medium (Gibco) at a final concentration of 2 ug/mL for 16 h. Negative control and positive control, CMV lysates (Meridian Bioscience, Cincinnati, OH, USA), and phytohemagglutinin (Sigma) were also included. The spots were quantified using an ELISpot analyzer (ImmunoSpot, Cleveland, OH, USA). Spot counts of negative control wells were subtracted from the peptide-stimulated wells to generate normalized results. These are reported as spot forming units (SFU) per million PBMCs. The responses higher than mean SFU counts plus 3 standard deviations (SD) of unstimulated wells, which is higher than 19 SFU per million PBMCs, were considered to be positive [19].

2.3. Statistical Analysis

Descriptive statistics were used to present demographic data, as well as clinical and laboratory parameters. Continuous variables were presented as the median and interquartile range (IQR). The Wilcoxon rank sum test was used to compare the continuous variables between the two groups. Chi-square test or Fisher exact test was used to compare the proportion between groups. Paired t test was used to compare the change in immunogenicity at week 12 and week 4 (reference) after the vaccine booster dose within group and was reported as geometric mean (GM) and geometric mean ratio (GMR) with 95% confidence interval (CI). Factors associated with anti-SARS-CoV-2 spike total antibody levels after the booster dose were analyzed by random-effects generalized least square regression (log transform). The correlation between anti-SARS-CoV-2 spike total antibodies and other immune responses was determined by the Spearman rank test. Statistical significance was defined as p-value < 0.05. STATA version 15.1 (Stata Corp., College Station, TX, USA) was used for statistical analysis.

3. Results

3.1. Participant Characteristics

From July to August 2021, 56 participants received the ChAdOx1 booster and 42 participants received the BNT162b2 booster. Blood was collected after 4 and 12 weeks post booster. The median age of participants who had CoronaVac was 41 (31–52) years, and most of them were female (Table 1). The median (IQR) interval after the second CoronaVac and ChAdOx1 booster was 88 (74–92) days and 113 (112–115) days for the BNT162b2 booster. Fourteen participants had a history of contact with COVID-19 patients without appropriate personal protective equipment and/or had symptoms suspected to be of COVID-19, and one participant was diagnosed with COVID-19 3 days after receiving the booster; the latter participant was excluded from the analysis. This infected participant had mild severity of the disease, with upper respiratory tract symptoms and no pneumonia.

3.2. Immune Response

3.2.1. Anti-SARS-CoV-2 Spike Total Antibodies

Anti-SARS-CoV-2 spike total antibodies after primary immunization and booster dose are shown in Table 2. Anti-SARS-CoV-2 spike total antibodies at 12 weeks after primary vaccination (2 doses of CoronaVac) were lower than after 4 weeks in both groups. After the participants received the ChAdOx1 booster, the anti-SARS-CoV-2 spike total antibodies at 4 and 12 weeks post booster were significantly lower than in the BNT162b2 booster group, p < 0.001. Anti-SARS-CoV-2 spike total antibodies rapidly decreased in the BNT162b2 booster group 12 weeks after booster (79% in the BNT162b2 group vs. 59% in the ChAdOx1 group) but were still higher than post-primary vaccination.

3.2.2. Surrogate Viral Neutralizing Antibody to Ancestral Type by GenScript and VOCs by Luminex

The sVNT to ancestral type at 12 weeks after primary vaccination (2 doses of CoronaVac) was lower than at 4 weeks in both groups. The median (IQR) of sVNT to ancestral type after booster with ChAdOx1 was significantly lower than the BNT162b2 booster group at 4 weeks after the booster (Table 2 and Figure 1). sVNT to each VOC was different. Overall, the median sVNT to each VOC in the BNT162b2 booster group was higher than the in ChAdOx1 booster group. In addition, the median of sVNT to wild type, Alpha, and Delta had similar patterns, whereas the Beta and Gamma variants had similar patterns. However, sVNT to Omicron was significantly lower than that of the other VOCs (Figure 2 and Supplement Table S1). The median (IQR) of sVNT to Omicron after booster with ChAdOx1 at 12 weeks was 26.6% (12.4–37.7%), which is significantly lower than booster with BNT162b2 (median 50.7 (IQR 34.6–71.9%), p < 0.001. The decline rate of sVNT to Omicron from week 4 to week 12 was 45% among BNT162b2 and 62% among ChAdOx1 participants; however, the decline rate of sVNT to other VOCs was only 6–20%. (Table 2 and Table 3).
According to the US FDA guidelines for a high titer of COVID-19 convalescent plasma, the % inhibition using the cPassTM SARS-CoV-2 Neutralization Antibody Detection Kit should be ≥68% [17]. None of the participants had sVNT > 68% to Omicron after primary CoronaVac immunization; however, the seroconversion rate increased to 83% and 35% after 4 weeks of BNT 162b2 and ChAdOx1 boosters, respectively. The seroconversion rate of other VOCs, except Omicron, was 94–100% in the BNT162b2 group and 80–100% in the ChAdOx1 group.

3.2.3. Assessment of T-Cell Response by ELISpot

The results of IFN-γ secreting T cell by Pool S1 and Pool S2 are shown in Figure 3 and Supplement Table S2. The responses had a similar pattern, which showed that there was a reduction in the immune response 12 weeks after primary immunization, increased immune response 4 weeks after the booster dose, and a reduction in the immune response 12 weeks after the booster. Percent positive responses after the booster at week 4 and week 12 were 100% and 90% with BNT162b2 and 80% and 80% with ChAdOx1, respectively. Although there was no significant difference between the BNT162b2 and ChAdOx1 booster groups, the median SFU after BNT162b2 was higher than after ChAdOx1 4 and 12 weeks post booster.

4. Discussion

This prospective observational cohort study reported the dynamics of antibodies against ancestral type and five VOCs, as well as T-cell responses in participants who received a primary immunization with CoronaVac and a booster with BNT162b2 or ChAdOx1. The results showed that there was a decrease in the immune response 12 weeks after primary immunization. Since November 2021, the variant has changed to Omicron, and a booster dose is recommended. At 4 weeks post booster, both BNT162b2 and ChAdOx1 resulted in significant boost of anti-SARS-CoV-2 spike total antibodies. The boost achieved by BNT162b is approximately 4 times higher than achieved by ChAdOx1, which correlates with higher sVNT against the Omicron strain (median 80.9% versus 54.5%). At 12 weeks post booster, anti-RBD rapidly declined which may reduce the effectiveness of the vaccines in preventing infection. Cell-mediated immune response measured by ELISpot assay showed that there was a similar T-cell immune response after booster in the ChAdOx1 group and BNT162b2 booster group. Cell-mediated immunity has been reported as an immune response that reduces the severity of disease [20].
Among the ChAdOx1 booster group, the anti-SARS-CoV-2 spike total antibodies to ancestral type SARS-CoV-2 and sVNT to ancestral type, Alpha, Beta, and Delta at 4 weeks post booster in this study were similar to the results of other studies [13,21]. However, the median of sVNT to omicron was 61.2% at 4 weeks post booster but declined to 26.6% at 12 weeks post booster, which indicates that one booster may not be able to prevent infection and, hence, an additional booster is needed. Among the BNT162b2 booster group, the immune response by neutralizing antibody to ancestral-type SARS-CoV-2, Delta, and Omicron at 4 weeks post booster was similar to that reported in other studies [10,22,23]. However, there was a significant decline in sVNT to omicron at 12 weeks after booster (median (IQR) sVNT to Omicron, 50.7% (IQR 34.6–71.9%)). The immune response in the BNT162b2 booster group was higher than that in the ChAdOx1 booster group 4 weeks post booster, which is in line with data from a previous study conducted in Brazil [11]. The mRNA vaccines have demonstrated a high neutralizing antibody response when compared to other vaccine platforms, including viral vector vaccines or inactivated virus vaccines used in primary immunization [24].
Interestingly, at weeks 12 after booster, the level of sVNT to ancestral type (cPassTM) was not as drastically decreased as the level of anti-SARS-CoV-2 spike total antibodies. This might be because the anti-SARS-CoV-2 spike total antibodies include non-neutralizing antibodies, which might have a different rate of decay. In this study, we used a commercial sVNT kit, which included test samples and control sera tested using a fixed dilution of 1:10. It is clear that all sera after booster have a high level of NAbs, reaching the saturation level (>95%) of NAbs dateable by this kit, as shown in our previous studies [25]. However, the sVNT of VOCs by multiplex sVNT (Luminex) detected a decrease in sVNT from week 4 to week 12.
T-cell responses to the spike protein increased substantially after a booster dose with either ChAdOx1 or BNT162b2. The response rate and the magnitude of T-cell response seem to be higher in the BNT162b2 booster group compared to the ChAdOx1 group, which supports the findings of a previous study [26]. It should be noted that there were some positive T-cell responses at baseline similar to a previous report [27]. However, after the primary vaccination (two doses of CoronaVac), the magnitude of T-cell responses was higher in every case, supporting the need for immunization. Although we did not measure T-cell responses to pool peptides derived from variant strains, the T-cell responses to Omicron should be reserved, as previously shown. [28,29] There was a correlation between specific T-cells and antibody responses to the spike protein (Supplement correlation graph), which is similar to the immune response seen in patients with primary infection [30].
The difference in the humoral immune response to each VOC occurred because the vaccines were designed to recognize the ancestral-type spike protein of SARS-CoV-2. Delta has 9 mutations, and Omicron has at least 46 highly prevalent mutations, which is more than any previous variant belonging to the S protein; therefore, it is not surprising to see a decrease in the humoral immune response toward other variants [4,31]. Whereas neutralizing antibodies target the spike protein and block cellular entry, the cytotoxic T cell plays an important role in eliminating virus-infected cells. The heterologous prime-boost strategy with different platforms has been shown to effectively improve immunogenicity [32]. The mRNA vaccine seems to induce a stronger immune response than the viral vector vaccine; however, the immune response to Omicron rapidly declines, which indicates that an additional booster dose is needed, first for health care workers, who are on the frontline in this pandemic, and then for all populations. A second booster should be an mRNA vaccine or a protein-based vaccine with adjuvant. For example, a protein-based vaccine with an adjuvant from Novavax shows promising results; it has high anti-RBD at the primary vaccination or after a booster [33,34].
This study has several strengths. First, it is a longitudinal cohort study. Second, this study includes antibody and T-cell response data from baseline to primary immunization, as well as post booster. Moreover, this study includes immune response data to all VOCs, especially the Delta and Omicron variants, which were predominantly circulating around the world at the time when the study was conducted. There are some limitations in this study. First, the study was non-randomized; therefore, a difference in age was reported between groups. However, we analyzed factors associated with anti-SARS-CoV-2 spike total antibodies (Supplement Table S3) and reported that the type of booster vaccine is significantly associated with this level (adjusted ratio 3.61 (95%CI 1.11–11.76, p 0.03)), whereas age and anti-SARS-CoV-2 spike antibodies prior to booster were not associated with this level. (Adjusted ratio 0.99 (95%CI 0.98–1.01, p 0.364, adjusted ratio 1.01 (95%CI 0.98–1.04, p 0.487)). Second, the sample size was small, so there was not enough power to study the clinical efficacy of the vaccine regimens. Lastly, the T-cell responses were only performed in a subset of the sample, and in this study, we did not test the T-cell responses to the spikes from different variants. However, a recent study showed that T-cell responses to the spikes of various variants were still detectable among vaccinated individuals [28,29,35].

5. Conclusions

The heterologous prime-boost regimen with CoronaVac followed by a booster with BNT162b2 or ChAdOx1 induced a significant increase in anti-SARS-CoV-2 spike total antibodies, neutralizing antibodies, and T cell responses. The BNT162b2 group had higher neutralizing antibodies against the Omicron strain, which is the predominant strain in the year 2022. The humoral immune response tends to decrease rapidly after 12 weeks post booster; therefore, health care workers, who are at high risk of virus acquisition, may need an additional booster dose (i.e., second booster) 3 months after the first booster dose. Future studies should assess the long-term immune response to determine the appropriate time to administer the second booster.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/vaccines10050639/s1, Figure S1: Correlation between total anti-SARS-CoV-2 and sVNT to ancestral type, Omicron, and ELISpot (Pool S1, S2); Table S1: Comparison of SARS-CoV-2 surrogate viral neutralizing antibody (%inhibition) and seroconversion rate of sVNT ≥ 68% to ancestral type and VOC by multiplex sVNT in participants who received primary immunization with CoronaVac and booster with ChAdOx1 or BNT 162b2.; Table S2: Comparison of T-cell response by ELISpot to Pool S1, and S2 in participants who received primary immunization with CoronaVac and booster with ChAdOx1 or BNT 162b2. Table S3: Factors associated with anti-SARS-CoV-2 spike total antibody level after the booster dose

Author Contributions

Conceptualization: T.P., S.W., N.H., L.-F.W. and O.P.; methodology: W.J. and N.C.; formal analysis: J.S.; investigation: P.S., A.J., S.N., N.T., V.R., C.W.T. and R.P.; data curation: W.J., N.C. and R.P.; writing—original draft preparation, W.J. and P.S.; writing—review and editing: N.C., A.J, S.N., N.T., V.R., R.P., J.S., S.W., T.P., N.H., L.-F.W. and O.P. All of the authors approved the final version submitted for publication and take responsibility for the statements made in the published article. All authors have read and agreed to the published version of the manuscript.

Funding

This study received funding from the Rachadaphiseksomphot Fund (RA(PO)002/64) of the Faculty of Medicine, Chulalongkorn University; the King Chulalongkorn Memorial Hospital Fund for research (HA-64-3300-21-024 and EC-65-C7-030); the National Research Council of Thailand (award nos. N35A640037 and N35A640452); NIH/NIAID/CREID (award 07-049-7012-52338); and the Thai Red Cross Fund. N.H. was supported by the Ratchadaphiseksomphot Matching Fund of the Faculty of Medicine, Chulalongkorn University. V.R. was supported by Postdoctoral Fellowship Scholarships, Ratchadapisek Somphot Fund, Chulalongkorn University. Part of this work was supported by the Biobank, Faculty of Medicine, Chulalongkorn University. Work carried at Duke-NUS was funded in part by grants from the National Medical Research Council (STPRG-FY19-001, COVID19RF-003, and OFLCG19May-0034).

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki and approved by the Research Ethics Review Committee, Faculty of Medicine, Chulalongkorn University (IRB No. 170/64), and was registered in the Thai Clinical Trial (TCTR20210325003).

Informed Consent Statement

Informed consent was obtained from all participants involved in the study.

Data Availability Statement

The supporting data for the findings of this study are available from the corresponding author upon reasonable request.

Acknowledgments

We would like to thank all of the participants at King Chulalongkorn Memorial Hospital for participating in this study, as well as the study team, especially Saithip Khumpiwdum, Phattharasuda Yodbutdee, and Thitima Maneepornpol, for their work on this study.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study.

References

  1. Jantarabenjakul, W.; Chantasrisawad, N.; Puthanakit, T.; Wacharapluesadee, S.; Hirankarn, N.; Ruenjaiman, V.; Paitoonpong, L.; Suwanpimolkul, G.; Torvorapanit, P.; Pradit, R.; et al. Short-term immune response after inactivated SARS-CoV-2 (CoronaVac(R), Sinovac) and ChAdOx1 nCoV-19 (Vaxzevria(R), Oxford-AstraZeneca) vaccinations in health care workers. Asian Pac. J. Allergy Immunol. 2021. [Google Scholar] [CrossRef]
  2. WHO. Available online: https://www.who.int/en/activities/tracking-SARS-CoV-2-variants/ (accessed on 5 March 2022).
  3. Krause, P.R.; Fleming, T.R.; Longini, I.M.; Peto, R.; Briand, S.; Heymann, D.L.; Beral, V.; Snape, M.D.; Rees, H.; Ropero, A.M.; et al. SARS-CoV-2 Variants and Vaccines. N. Engl. J. Med. 2021, 385, 179–186. [Google Scholar] [CrossRef] [PubMed]
  4. Garcia-Beltran, W.F.; Denis, K.J.S.; Hoelzemer, A.; Lam, E.C.; Nitido, A.D.; Sheehan, M.L.; Berrios, C.; Ofoman, O.; Chang, C.C.; Hauser, B.M.; et al. mRNA-based COVID-19 vaccine boosters induce neutralizing immunity against SARS-CoV-2 Omicron variant. Cell 2022, 185, 457–466. [Google Scholar] [CrossRef] [PubMed]
  5. Cele, S.; Jackson, L.; Khoury, D.S.; Khan, K.; Moyo-Gwete, T.; Tegally, H.; San, J.E.; Cromer, D.; Scheepers, C.; Amoako, D.G.; et al. Omicron extensively but incompletely escapes Pfizer BNT162b2 neutralization. Nature 2022, 602, 654–656. [Google Scholar] [CrossRef] [PubMed]
  6. Liu, X.; Shaw, R.H.; Stuart, A.S.V.; Greenland, M.; Aley, P.K.; Andrews, N.J.; Cameron, J.C.; Charlton, S.; Clutterbuck, E.A.; Collins, A.M.; et al. Safety and immunogenicity of heterologous versus homologous prime-boost schedules with an adenoviral vectored and mRNA COVID-19 vaccine (Com-COV): A single-blind, randomised, non-inferiority trial. Lancet 2021, 398, 856–869. [Google Scholar] [CrossRef]
  7. Nguyen, T.T.; Quach, T.H.T.; Tran, T.M.; Phuoc, H.N.; Nguyen, H.T.; Vo, T.K.; Van Vo, G. Reactogenicity and immunogenicity of heterologous prime-boost immunization with COVID-19 vaccine. Biomed. Pharmacother. 2022, 147, 112650. [Google Scholar] [CrossRef] [PubMed]
  8. Zeng, G.; Wu, Q.; Pan, H.; Li, M.; Yang, J.; Wang, L.; Wu, Z.; Jiang, D.; Deng, X.; Chu, K.; et al. Immunogenicity and safety of a third dose of CoronaVac, and immune persistence of a two-dose schedule, in healthy adults: Interim results from two single-centre, double-blind, randomised, placebo-controlled phase 2 clinical trials. Lancet Infect. Dis. 2021, 22, 483–495. [Google Scholar] [CrossRef]
  9. Liu, Y.; Zeng, Q.; Deng, C.; Li, M.; Li, L.; Liu, D.; Liu, M.; Ruan, X.; Mei, J.; Mo, R.; et al. Robust induction of B cell and T cell responses by a third dose of inactivated SARS-CoV-2 vaccine. Cell Discov. 2022, 8, 10. [Google Scholar] [CrossRef] [PubMed]
  10. Keskin, A.U.; Bolukcu, S.; Ciragil, P.; Topkaya, A.E. SARS-CoV-2 specific antibody responses after third CoronaVac or BNT162b2 vaccine following two-dose CoronaVac vaccine regimen. J. Med. Virol. 2022, 94, 39–41. [Google Scholar] [CrossRef]
  11. Costa Clemens, S.A.; Weckx, L.; Clemens, R.; Almeida Mendes, A.V.; Ramos Souza, A.; Silveira, M.B.; da Guarda, S.N.F.; de Nobrega, M.M.; de Moraes Pinto, M.I.; Gonzalez, I.G.; et al. Heterologous versus homologous COVID-19 booster vaccination in previous recipients of two doses of CoronaVac COVID-19 vaccine in Brazil (RHH-001): A phase 4, non-inferiority, single blind, randomised study. Lancet 2022, 399, 521–529. [Google Scholar] [CrossRef]
  12. Çağlayan, D.; Süner, A.F.; Şiyve, N.; Güzel, I.; Irmak, Ç.; Işik, E.; Appak, Ö.; Çelik, M.; Öztürk, G.; Çavuş, S.A.; et al. An analysis of antibody response following the second dose of CoronaVac and humoral response after booster dose with BNT162b2 or CoronaVac among healthcare workers in Turkey. J. Med. Virol. 2022, 94, 2212–2221. [Google Scholar] [CrossRef] [PubMed]
  13. Yorsaeng, R.; Suntronwong, N.; Phowatthanasathian, H.; Assawakosri, S.; Kanokudom, S.; Thongmee, T.; Vichaiwattana, P.; Auphimai, C.; Wongsrisang, L.; Srimuan, D.; et al. Immunogenicity of a third dose viral-vectored COVID-19 vaccine after receiving two-dose inactivated vaccines in healthy adults. Vaccine 2021, 40, 524–530. [Google Scholar] [CrossRef] [PubMed]
  14. Tan, C.W.; Chia, W.N.; Qin, X.; Liu, P.; Chen, M.I.C.; Tiu, C.; Hu, Z.; Chen, V.C.W.; Young, B.E.; Sia, W.R.; et al. A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction. Nat. Biotechnol. 2020, 38, 1073–1078. [Google Scholar] [CrossRef] [PubMed]
  15. Elecsys Anti-SARS-CoV-2. Available online: https://www.fda.gov/media/137605/download (accessed on 14 March 2021).
  16. Jochum, S.; Kirste, I.; Hortsch, S.; Grunert, V.P.; Legault, H.; Eichenlaub, U.; Kashlan, B.; Pajon, R. Clinical Utility of Elecsys Anti-SARS-CoV-2 S Assay in COVID-19 Vaccination: An Exploratory Analysis of the mRNA-1273 Phase 1 Trial. Front. Immunol. 2021, 12, 798117. [Google Scholar] [CrossRef] [PubMed]
  17. De Santis, G.C.; Mendrone, A.; Langhi, D., Jr.; Covas, D.T.; Fabron, A., Jr.; Cortez, A.J.P.; Dinardo, C.L.; Ubiali, E.M.A.; Marques, J.F.C., Jr.; Bordin, J.O.; et al. Suggested guidelines for convalescent plasma therapy for the treatment of COVID-19. Hematol. Transfus. Cell Ther. 2021, 43, 212–213. [Google Scholar] [CrossRef]
  18. Bossart, K.N.; McEachern, J.A.; Hickey, A.C.; Choudhry, V.; Dimitrov, D.S.; Eaton, B.T.; Wang, L.-F. Neutralization assays for differential henipavirus serology using Bio-Plex Protein Array Systems. J. Virol. Methods 2007, 142, 29–40. [Google Scholar] [CrossRef]
  19. Lehmann, A.A.; Kirchenbaum, G.A.; Zhang, T.; Reche, P.A.; Lehmann, P.V. Deconvoluting the T Cell Response to SARS-CoV-2: Specificity Versus Chance and Cognate Cross-Reactivity. Front. Immunol. 2021, 12, 635942. [Google Scholar] [CrossRef]
  20. Kudlay, D.; Kofiadi, I.; Khaitov, M. Peculiarities of the T Cell Immune Response in COVID-19. Vaccines 2022, 10, 242. [Google Scholar] [CrossRef]
  21. Prasithsirikul, W.; Pongpirul, K.; Nopsopon, T.; Phutrakool, P.; Pongpirul, W.; Samuthpongtorn, C.; Suwanwattana, P.; Jongkaewwattana, A. Immunogenicity of ChAdOx1 nCoV-19 Booster Vaccination Following Two CoronaVac Shots in Healthcare Workers. Vaccines 2022, 10, 217. [Google Scholar] [CrossRef]
  22. Pérez-Then, E.; Lucas, C.; Monteiro, V.S.; Miric, M.; Brache, V.; Cochon, L.; Vogels, C.B.; Malik, A.A.; De la Cruz, E.; Jorge, A.; et al. Neutralizing antibodies against the SARS-CoV-2 Delta and Omicron variants following heterologous CoronaVac plus BNT162b2 booster vaccination. Nat. Med. 2022, 28, 481–485. [Google Scholar] [CrossRef]
  23. Pérez-Then, E.; Lucas, C.; Monteiro, V.S.; Miric, M.; Brache, V.; Cochon, L.; Vogels, C.B.; De la Cruz, E.; Jorge, A.; De los Santos, M.; et al. Immunogenicity of heterologous BNT162b2 booster in fully vaccinated individuals with CoronaVac against SARS-CoV-2 variants Delta and Omicron: The Dominican Republic Experience. medRxiv 2021. [Google Scholar] [CrossRef]
  24. Sadarangani, M.; Marchant, A.; Kollmann, T.R. Immunological mechanisms of vaccine-induced protection against COVID-19 in humans. Nat. Rev. Immunol. 2021, 21, 475–484. [Google Scholar] [CrossRef] [PubMed]
  25. Zhu, F.; Althaus, T.; Tan, C.W.; Costantini, A.; Chia, W.N.; Chau, N.V.V.; Mattiuzzo, G.; Rose, N.J.; Voiglio, E.; Wang, L.F. WHO international standard for SARS-CoV-2 antibodies to determine markers of protection. Lancet Microbe 2022, 3, e81–e82. [Google Scholar] [CrossRef]
  26. Kanokudom, S.; Assawakosri, S.; Suntronwong, N.; Auphimai, C.; Nilyanimit, P.; Vichaiwattana, P.; Thongmee, T.; Yorsaeng, R.; Srimuan, D.; Thatsanatorn, T.; et al. Safety and Immunogenicity of the Third Booster Dose with Inactivated, Viral Vector, and mRNA COVID-19 Vaccines in Fully Immunized Healthy Adults with Inactivated Vaccine. Vaccines 2022, 10, 86. [Google Scholar] [CrossRef] [PubMed]
  27. Braun, J.; Loyal, L.; Frentsch, M.; Wendisch, D.; Georg, P.; Kurth, F.; Hippenstiel, S.; Dingeldey, M.; Kruse, B.; Fauchere, F.; et al. SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19. Nature 2020, 587, 270–274. [Google Scholar] [CrossRef] [PubMed]
  28. Keeton, R.; Tincho, M.B.; Ngomti, A.; Baguma, R.; Benede, N.; Suzuki, A.; Khan, K.; Cele, S.; Bernstein, M.; Karim, F.; et al. T cell responses to SARS-CoV-2 spike cross-recognize Omicron. Nature 2022, 603, 488–492. [Google Scholar] [CrossRef]
  29. Liu, J.; Chandrashekar, A.; Sellers, D.; Barrett, J.; Jacob-Dolan, C.; Lifton, M.; McMahan, K.; Sciacca, M.; VanWyk, H.; Wu, C.; et al. Vaccines elicit highly conserved cellular immunity to SARS-CoV-2 Omicron. Nature 2022, 603, 493–496. [Google Scholar] [CrossRef]
  30. Zuo, J.; Dowell, A.C.; Pearce, H.; Verma, K.; Long, H.M.; Begum, J.; Aiano, F.; Amin-Chowdhury, Z.; Hoschler, K.; Brooks, T.; et al. Robust SARS-CoV-2-specific T cell immunity is maintained at 6 months following primary infection. Nat. Immunol. 2021, 22, 620–626. [Google Scholar] [CrossRef]
  31. Kannan, S.R.; Spratt, A.N.; Sharma, K.; Chand, H.S.; Byrareddy, S.N.; Singh, K. Omicron SARS-CoV-2 variant: Unique features and their impact on pre-existing antibodies. J. Autoimmun. 2022, 126, 102779. [Google Scholar] [CrossRef]
  32. He, Q.; Mao, Q.; An, C.; Zhang, J.; Gao, F.; Bian, L.; Li, C.; Liang, Z.; Xu, M.; Wang, J. Heterologous prime-boost: Breaking the protective immune response bottleneck of COVID-19 vaccine candidates. Emerg. Microbes Infect. 2021, 10, 629–637. [Google Scholar] [CrossRef]
  33. Heath, P.T.; Galiza, E.P.; Baxter, D.N.; Boffito, M.; Browne, D.; Burns, F.; Chadwick, D.R.; Clark, R.; Cosgrove, C.; Galloway, J.; et al. Safety and Efficacy of NVX-CoV2373 Covid-19 Vaccine. N. Engl. J. Med. 2021, 385, 1172–1183. [Google Scholar] [CrossRef] [PubMed]
  34. Munro, A.P.S.; Janani, L.; Cornelius, V.; Aley, P.K.; Babbage, G.; Baxter, D.; Bula, M.; Cathie, K.; Chatterjee, K.; Dodd, K.; et al. Safety and immunogenicity of seven COVID-19 vaccines as a third dose (booster) following two doses of ChAdOx1 nCov-19 or BNT162b2 in the UK (COV-BOOST): A blinded, multicentre, randomised, controlled, phase 2 trial. Lancet 2021, 398, 2258–2276. [Google Scholar] [CrossRef]
  35. Gallagher, K.M.; Leick, M.B.; Larson, R.C.; Berger, T.R.; Katsis, K.; Yam, J.Y.; Brini, G.; Grauwet, K.; MGH COVID-19 Collection & Processing Team; Maus, M.V. SARS-CoV-2 T-cell immunity to variants of concern following vaccination. bioRxiv 2021. [Google Scholar] [CrossRef]
Vaccines 10 00639 g001 550
Figure 1. Dynamic of immune response of participants who received primary CoronaVac vaccination and were boosted with BNT162b2 or ChAdOx1 at week 12 for (A) anti-SARS-CoV-2 spike total antibodies (U/mL) by Elecsys and (B) surrogate neutralizing antibody to ancestral type (% inhibition) by GenScript at 4 and 12 weeks post booster.
Figure 1. Dynamic of immune response of participants who received primary CoronaVac vaccination and were boosted with BNT162b2 or ChAdOx1 at week 12 for (A) anti-SARS-CoV-2 spike total antibodies (U/mL) by Elecsys and (B) surrogate neutralizing antibody to ancestral type (% inhibition) by GenScript at 4 and 12 weeks post booster.
Vaccines 10 00639 g001
Vaccines 10 00639 g002 550
Figure 2. Dynamics of immune response surrogate neutralizing antibody (sVNT) to ancestral type, Delta, Omicron, Alpha, Beta, and Gamma in participants who received primary CoronaVac vaccination and were boosted with BNT162b2 or ChAdOx1 by multiplex sVNT.
Figure 2. Dynamics of immune response surrogate neutralizing antibody (sVNT) to ancestral type, Delta, Omicron, Alpha, Beta, and Gamma in participants who received primary CoronaVac vaccination and were boosted with BNT162b2 or ChAdOx1 by multiplex sVNT.
Vaccines 10 00639 g002
Vaccines 10 00639 g003 550
Figure 3. Dynamics of T-cell response by ELISpot (A) Pool S1, and (B) Pool S2 in participants who received primary CoronaVac vaccination and were boosted with BNT162b2 or ChAdOx1.
Figure 3. Dynamics of T-cell response by ELISpot (A) Pool S1, and (B) Pool S2 in participants who received primary CoronaVac vaccination and were boosted with BNT162b2 or ChAdOx1.
Vaccines 10 00639 g003
Table
Table 1. Baseline characteristics of the participants who received primary immunization with CoronaVac and booster with ChAdOx1 or BNT162b2.
Table 1. Baseline characteristics of the participants who received primary immunization with CoronaVac and booster with ChAdOx1 or BNT162b2.
CharacteristicsChAdOx1 BoosterBNT 162b2 Boosterp-Value
N = 56N = 42
Age, years, median (IQR)47
(34–53)		32
(30–45)		0.001
Age group, n(%) 0.01
20–30 years7 (12.5)15 (35.7)
31–40 years12 (21.4)14 (33.3)
41–50 years13 (23.2)5 (11.9)
51–60 years24 (42.9)8 (19.1)
Female, n(%)44 (78.6)34 (81)0.005
BMI (kg/m2),
median (IQR)		22.6
(20.3–25.9)		21.5
(20–25.8)		0.45
IQR, interquartile range; BMI, body mass index.
Table
Table 2. Comparison of anti-SARS-CoV-2 spike total antibodies (U/mL) by Elecsys and surrogate viral neutralizing antibody to ancestral strain by GenScript in participants who received primary immunization with CoronaVac and booster with ChAdOx1 or BNT 162b2.
Table 2. Comparison of anti-SARS-CoV-2 spike total antibodies (U/mL) by Elecsys and surrogate viral neutralizing antibody to ancestral strain by GenScript in participants who received primary immunization with CoronaVac and booster with ChAdOx1 or BNT 162b2.
Median
(IQR)		Anti-SARS-CoV-2 Spike Total Antibodies
(U/mL)		Surrogate Viral Neutralizing Antibody to Ancestral Type (%Inhibition) by GenScript
Primary immunization(N = 56)(N = 42)p-value(N = 56)(N = 42)p-value
Week 4179
(92–301)		242
(163–462)		0.0466.8
(47.4–86.1)		83.4
(66.8–91.0)		0.04
Week 1256
(41–126)		117
(61–198)		0.00139.4
(18.6–57.8)		41.3
(31.7–59.2)		0.26
BoosterChAdOx1BNT162b2p-valueChAdOx1BNT162b2p-value
Week 47768
(5349–11,142)		25,129
(17,531–39,434)		<0.00198.1
(97.9–98.2)		98.5
(98.5–98.6)		<0.001
Week 123139
(2185–4660)		6558
(3836–8885)		<0.00197.9
(95.8–98.1)		97.9
(97.8–98.2)		0.03
Table
Table 3. Comparison of the changes in anti-SARS-CoV-2 spike total antibodies and surrogate viral neutralizing antibody (sVNT) by multiplex sVNT between week 4 and week 12 after the booster dose.
Table 3. Comparison of the changes in anti-SARS-CoV-2 spike total antibodies and surrogate viral neutralizing antibody (sVNT) by multiplex sVNT between week 4 and week 12 after the booster dose.
Post BoosterPrimary Immunization with CoronaVac and Booster with ChAdOx1Primary Immunization with CoronaVac and Booster with BNT162b2
GMGMR%
Decrease		GMGMR%
Decrease
Anti-SARS-CoV-2 spike total antibodies
week 47292.9 (6049.7–8791.6)ref 29,268.9 (23,921.4–35,812.1)ref
week 122960.5 (2421.0–3620.2)0.41 (0.37–0.45)59%6200.0 (5043.5–7621.8)0.21 (0.18–0.25)79%
Surrogate viral neutralizing antibody(sVNT)
Ancestral type
week 492.8 (90.3–95.3)Ref 99.5 (99.4–99.7)ref
week 1279.2 (74.1–84.6)0.85 (0.81–0.89)15%93.8 (91.7–96.0)0.94 (0.92–0.96)6%
Alpha
week 490.3 (87.0–93.8)ref 90.3 (87.0–93.8)ref
week 1274.8 (69.4–80.6)0.83 (0.79–0.87)17%74.8 (69.4–80.6)0.83 (0.79–0.87)17%
Beta
week 479.7 (75.0–84.8)ref 96.4 (95.7–97.2)ref
week 1258.1 (52.3–64.4)0.73 (0.68–0.78)27%85.2 (81.5–89.0)0.88 (0.85–0.92)12%
Gamma
week 481.9 (77.4–86.7)ref 97.1 (96.4–97.8)ref
week 1259.8 (53.8–66.4)0.73 (0.68–0.78)27%86.7 (83.3–90.3)0.89 (0.86–0.93)11%
Delta
week 489.6 (86.3–93.1)ref 99.1 (98.9–99.3)ref
week 1271.4 (65.6–77.7)0.8 (0.75–0.84)20%91.1 (88.3–94)0.92 (0.89–0.95)8%
Omicron
week 454.5 (48.6–61.3)ref 80.9 (75.1–87.1)ref
week 1220.8 (15.3–28.2)0.38 (0.29–0.49)62%44.4 (35.3–55.7)0.55 (0.43–0.69)45%
GM, geometric mean; GMR, geometric mean ratio.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Jantarabenjakul, W.; Sodsai, P.; Chantasrisawad, N.; Jitsatja, A.; Ninwattana, S.; Thippamom, N.; Ruenjaiman, V.; Tan, C.W.; Pradit, R.; Sophonphan, J.; et al. Dynamics of Neutralizing Antibody and T-Cell Responses to SARS-CoV-2 and Variants of Concern after Primary Immunization with CoronaVac and Booster with BNT162b2 or ChAdOx1 in Health Care Workers. Vaccines 2022, 10, 639. https://doi.org/10.3390/vaccines10050639

AMA Style

Jantarabenjakul W, Sodsai P, Chantasrisawad N, Jitsatja A, Ninwattana S, Thippamom N, Ruenjaiman V, Tan CW, Pradit R, Sophonphan J, et al. Dynamics of Neutralizing Antibody and T-Cell Responses to SARS-CoV-2 and Variants of Concern after Primary Immunization with CoronaVac and Booster with BNT162b2 or ChAdOx1 in Health Care Workers. Vaccines. 2022; 10(5):639. https://doi.org/10.3390/vaccines10050639

Chicago/Turabian Style

Jantarabenjakul, Watsamon, Pimpayao Sodsai, Napaporn Chantasrisawad, Anusara Jitsatja, Sasiprapa Ninwattana, Nattakarn Thippamom, Vichaya Ruenjaiman, Chee Wah Tan, Rakchanok Pradit, Jiratchaya Sophonphan, and et al. 2022. "Dynamics of Neutralizing Antibody and T-Cell Responses to SARS-CoV-2 and Variants of Concern after Primary Immunization with CoronaVac and Booster with BNT162b2 or ChAdOx1 in Health Care Workers" Vaccines 10, no. 5: 639. https://doi.org/10.3390/vaccines10050639

APA Style

Jantarabenjakul, W., Sodsai, P., Chantasrisawad, N., Jitsatja, A., Ninwattana, S., Thippamom, N., Ruenjaiman, V., Tan, C. W., Pradit, R., Sophonphan, J., Wacharapluesadee, S., Wang, L. -F., Puthanakit, T., Hirankarn, N., & Putcharoen, O. (2022). Dynamics of Neutralizing Antibody and T-Cell Responses to SARS-CoV-2 and Variants of Concern after Primary Immunization with CoronaVac and Booster with BNT162b2 or ChAdOx1 in Health Care Workers. Vaccines, 10(5), 639. https://doi.org/10.3390/vaccines10050639

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop