The Functional Mechanism of BP9 in Promoting B Cell Differentiation and Inducing Antigen Presentation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Peptides, Antigens, and Cells
2.2. Experimental Animals
2.3. Main Reagents
2.4. Main Instruments and Equipment
2.5. Vaccine Preparation and Animal Immunization Protocol
2.6. Serum-Specific Antibody Level Detection
2.7. MTT Assay/Splenic Lymphocyte Activity Assessment
2.8. FCM
2.9. RNA Sequencing of B-Cells
2.10. HD11 Cell Treatment and Detection
2.11. Sample Preparation and miRNA Sequencing
2.12. Verification of DEGs in HD11 Cells with BP9 Treatment
2.13. Data Statistics and Analysis
3. Results
3.1. BP9 Enhanced the Antibody Production and the Proliferation of Splenic Lymphocytes In Vivo
3.2. BP9 Stimulated the Differentiation of B Cells In Vivo
3.3. BP9 Induced Differential Expressions of Multiple Genes and Transcription Factors
3.3.1. Statistical Assessment of Differentially Expressed Genes
3.3.2. BP9 Enhanced Various Antigen Presentation Pathways
3.3.3. BP9 Activated the Multiple Biological Processes in B Cells
3.3.4. BP9 Activated the Multiple Transcript Factors in B Cells
3.4. BP9 Stimulated the Cytokine Expressions in Chicken Macrophages
3.5. BP9 Promoted Various Signal and Biological Processes in Chicken Macrophages
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | NCBI Reference | Sequence (5′-3′) |
---|---|---|
IL-1β-F | NM_204524.2 | ACCCGCTTCATCTTCTACCG |
IL-1β-R | TCAGCGCCCACTTAGCTTG | |
IL-6-F | NM_204628.2 | AGGACGAGATGTGCAAGAAGTTC |
IL-6-R | TTGGGCAGGTTGAGGTTGTT | |
IL-10-F | NM_001004414.4 | CGCTGTCACCGCTTCTTCA |
IL-10-R | CGTCTCCTTGATCTGCTTGATG | |
iNOS-F | U46504.1 | AGGCCAAACATCCTGGAGGTC |
iNOS-R | TCATAGAGACGCTGCTGCCAG | |
IFN-α-F | GU119896.1 | GGACATGGCTCCCACACTAC |
IFN-α-R | GGCTGCTGAGGATTTTGAAGA | |
β-actin-F | NM_205518.2 | AGACATCAGGGTGTGATGGTTGGT |
β-actin-R | TGGTGACAATACCGTGTTCAATGG |
Primer Name | Sequence (5′-3′) |
---|---|
gga-miR-26a-5p | TCCAGCTGGGTTCAAGTAATCCAGG |
gga-miR-210a-3p | CTGTGCGTGTGACAGCGGCTAA |
gga-miR-218-5p | TTGTGCTTGATCTAACCATGT |
5sR-F | GTCTACGGCCATACCACCCTGAAC |
Name | Q-Value | Up Count | Down Count |
---|---|---|---|
Hematopoietic cell lineage | 5.0588 × 10−9 | 3 | 30 |
Cytokine–cytokine receptor interaction | 1.4634 × 10−8 | 13 | 53 |
ECM–receptor interaction | 6.8286 × 10−6 | 6 | 20 |
Cell adhesion molecules (CAMs) | 3.2955 × 10−5 | 5 | 32 |
Protein digestion and absorption | 0.00014185 | 3 | 23 |
Tryptophan metabolism | 0.00014185 | 3 | 14 |
ABC transporters | 0.00020629 | 1 | 17 |
Malaria | 0.00027825 | 4 | 13 |
Steroid biosynthesis | 0.00059044 | 0 | 10 |
Glycine, serine, and threonine metabolism | 0.00158069 | 3 | 17 |
African trypanosomiasis | 0.00225022 | 3 | 9 |
Nitrogen metabolism | 0.00231943 | 2 | 8 |
Glycerolipid metabolism | 0.0034593 | 2 | 18 |
Osteoclast differentiation | 0.00443348 | 8 | 21 |
Axon guidance | 0.0054491 | 8 | 31 |
Pathways in cancer | 0.005574 | 14 | 92 |
Porphyrin and chlorophyll metabolism | 0.00712266 | 0 | 13 |
Glutathione metabolism | 0.00831065 | 2 | 22 |
Complement and coagulation cascades | 0.00896268 | 7 | 14 |
Ether lipid metabolism | 0.00942156 | 3 | 12 |
Valine, leucine, and isoleucine degradation | 0.01069113 | 3 | 12 |
Alanine, aspartate, and glutamate metabolism | 0.01326763 | 1 | 12 |
Glycerophospholipid metabolism | 0.01326763 | 4 | 19 |
Metabolism of xenobiotics by cytochrome P450 | 0.01326763 | 2 | 19 |
Pancreatic secretion | 0.01326763 | 1 | 24 |
cAMP signaling pathway | 0.02294556 | 6 | 33 |
Fat digestion and absorption | 0.02354943 | 0 | 12 |
MAPK signaling pathway | 0.02362328 | 9 | 44 |
microRNAs in cancer | 0.025189 | 6 | 27 |
Neuroactive ligand–receptor interaction | 0.04037019 | 12 | 39 |
Fluid shear stress and atherosclerosis | 0.04050935 | 7 | 33 |
Relaxin signaling pathway | 0.04050935 | 4 | 22 |
Mineral absorption | 0.04379525 | 0 | 13 |
Bile secretion | 0.04465393 | 0 | 17 |
Vitamin digestion and absorption | 0.04546349 | 2 | 6 |
Glyoxylate and dicarboxylate metabolism | 0.04715224 | 1 | 11 |
Adrenergic signaling in cardiomyocytes | 0.04788709 | 8 | 21 |
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Hu, J.; Zhang, Z.; Cai, J.; Hao, S.; Li, C.; Feng, X. The Functional Mechanism of BP9 in Promoting B Cell Differentiation and Inducing Antigen Presentation. Vaccines 2024, 12, 607. https://doi.org/10.3390/vaccines12060607
Hu J, Zhang Z, Cai J, Hao S, Li C, Feng X. The Functional Mechanism of BP9 in Promoting B Cell Differentiation and Inducing Antigen Presentation. Vaccines. 2024; 12(6):607. https://doi.org/10.3390/vaccines12060607
Chicago/Turabian StyleHu, Jianing, Ze Zhang, Jiaxi Cai, Shanshan Hao, Chenfei Li, and Xiuli Feng. 2024. "The Functional Mechanism of BP9 in Promoting B Cell Differentiation and Inducing Antigen Presentation" Vaccines 12, no. 6: 607. https://doi.org/10.3390/vaccines12060607