Viral Vector-Based Chlamydia trachomatis Vaccines Encoding CTH522 Induce Distinct Immune Responses in C57BL/6J and HLA Transgenic Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Cells
2.3. Plasmid Construction and Recombinant Virus Generation
2.4. Viruses
2.5. PCR Analysis of Recombinant MVAs
2.6. SDS-PAGE and Western Blot
2.7. Immunofluorescence Microscopy and Flow Cytometry
2.8. Immunization and Tissue Collection
2.9. Peptides
2.10. Cellular-Specific Immune Response Analysis
2.11. Intracellular Staining
2.12. Enzyme-Linked Immunosorbent Assay
2.13. Statistical Analysis
3. Results
3.1. Generation and In Vitro Characterization of MVA-CTH522
3.2. Generation and In Vitro Characterization of MVA-spCTH522 and MVA-CTH522:B7
3.3. MVA-spCTH522 and MVA-CTH522:B7 Induced Systemic CD4+ But Not CD8+ T-Cell Responses against CTH522 in C57BL/6J Mice
3.4. MVA-CTH522:B7 But Not MVA-spCTH522 Induced Humoral Responses in C57BL/6J Mice
3.5. MVA-CTH522:B7 Induced T-Cell and Antibody Responses in HLA Transgenic Mice
3.6. MVA-spCTH522 and MVA-CTH522:B7 Induced Multifunctional CD8+ T Cell in HLA Transgenic Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Andreacchio, G.; Longo, Y.; Moreno Mascaraque, S.; Anandasothy, K.; Tofan, S.; Özün, E.; Wilschrey, L.; Ptok, J.; Huynh, D.T.; Luirink, J.; et al. Viral Vector-Based Chlamydia trachomatis Vaccines Encoding CTH522 Induce Distinct Immune Responses in C57BL/6J and HLA Transgenic Mice. Vaccines 2024, 12, 944. https://doi.org/10.3390/vaccines12080944
Andreacchio G, Longo Y, Moreno Mascaraque S, Anandasothy K, Tofan S, Özün E, Wilschrey L, Ptok J, Huynh DT, Luirink J, et al. Viral Vector-Based Chlamydia trachomatis Vaccines Encoding CTH522 Induce Distinct Immune Responses in C57BL/6J and HLA Transgenic Mice. Vaccines. 2024; 12(8):944. https://doi.org/10.3390/vaccines12080944
Chicago/Turabian StyleAndreacchio, Giuseppe, Ylenia Longo, Sara Moreno Mascaraque, Kartikan Anandasothy, Sarah Tofan, Esma Özün, Lena Wilschrey, Johannes Ptok, Dung T. Huynh, Joen Luirink, and et al. 2024. "Viral Vector-Based Chlamydia trachomatis Vaccines Encoding CTH522 Induce Distinct Immune Responses in C57BL/6J and HLA Transgenic Mice" Vaccines 12, no. 8: 944. https://doi.org/10.3390/vaccines12080944