Impact of Oil-in-Water Adjuvanted β-Glucan on Innate Immune Memory in Piglets
Abstract
:1. Introduction
2. Material and Methods
2.1. In Vitro Assessment of the Immunomodulatory Effects of Various β-Glucans
2.2. Ethics and Animal Experimental Conditions
2.3. Treatment Groups
2.4. Blood Sample Preparation
2.5. Cytokine Measurements
2.6. Antibody Measurements
2.7. Bulk-RNA Sequencing and Analysis
2.8. Statistical Analysis
3. Results
3.1. In Vitro Selection of Immunostimulatory β-Glucans
3.2. Safety Parameters
3.3. Cytokine and Antibody Profiles
3.4. Transcriptomic Profile of Leukocytes Post-Stimulation
3.5. Transcriptomic Profile of Leukocytes Post-Vaccination
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Score | Description | |
---|---|---|
0 | Normal | no more than a visible injection site less than about 0.5 diameter zone of redness surrounding injection site |
1 | Mild | 0.5–2 cm diameter discoloration no distinct palpable swelling may be irritation (occasional rubbing at injection site) |
2 | Moderate | 2–5 cm diameter discoloration (or) palpable swelling may be irritation (persistent rubbing at injection site) |
3 | Sever | >5 cm diameter discoloration (and) visible and palpable swelling irritation and pain (persistent rubbing at injection site, withdrawal and vocalization upon palpation may be abscess, exudate |
Treatment | PBS | Adj | Adj_BG | OBG |
---|---|---|---|---|
Post-stimulation | ||||
D1_PS | 978 | 1045 | 1376 | 2617 |
D3_PS | 703 | 6034 | 3771 | 3263 |
D7_PS | 5939 | 5057 | 4149 | 4153 |
D14_PS | 5008 | 5412 | 3891 | 4001 |
Post-vaccination | ||||
D1_PV | 1471 | 5119 | 4377 | 5238 |
D7_PV | 3934 | 3972 | 3650 | 4222 |
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Ardali, R.; Garcia-Nicolas, O.; Ollagnier, C.; Sánchez Carvajal, J.M.; Levy, M.; Yvernault, P.; de Aboim Borges Fialho de Brito, F.; Summerfield, A. Impact of Oil-in-Water Adjuvanted β-Glucan on Innate Immune Memory in Piglets. Vaccines 2024, 12, 982. https://doi.org/10.3390/vaccines12090982
Ardali R, Garcia-Nicolas O, Ollagnier C, Sánchez Carvajal JM, Levy M, Yvernault P, de Aboim Borges Fialho de Brito F, Summerfield A. Impact of Oil-in-Water Adjuvanted β-Glucan on Innate Immune Memory in Piglets. Vaccines. 2024; 12(9):982. https://doi.org/10.3390/vaccines12090982
Chicago/Turabian StyleArdali, Razieh, Obdulio Garcia-Nicolas, Catherine Ollagnier, José María Sánchez Carvajal, Maria Levy, Pauline Yvernault, Francisco de Aboim Borges Fialho de Brito, and Artur Summerfield. 2024. "Impact of Oil-in-Water Adjuvanted β-Glucan on Innate Immune Memory in Piglets" Vaccines 12, no. 9: 982. https://doi.org/10.3390/vaccines12090982