Influence of Various Forms of Foliar Application on Root Yield and Technological Quality of Sugar Beet

Abstract

The Green Deal adopted by the European Commission assumes a significant reduction in the use of pesticides and synthetic fertilizers. It is necessary to search for environmentally safe technologies that will prevent a reduction in crop yield. One of such methods, which was examined in the study, is the foliar application of silicon, which can have a positive effect on root yield and its quality. In the period 2017–2019, a field experiment was carried out in which the effectiveness of the application of various forms of silicon (orthosilicic acid, a mixture of orthosilicic and polysilicic acid and calcium silicate) in sugar beet cultivation was assessed. The applied treatments of foliar application increased the root yield by 10.7–11.7%, the biological sugar yield by 8.4–12% and the pure sugar yield by 7.2–11.8% as compared to the control treatment. The differences between the individual treatments in terms of these characteristics were insignificant. Their impacts on the technological quality of roots (content of sugar, α-amino nitrogen, potassium and sodium) were different.

1. Introduction

Sugar beet is the main raw material for sugar production in Europe. In 2019, it covered an area of 1.64 million ha in the European Union (28 countries) [1]. The production of sugar beet is limited by abiotic and biotic stress factors. The “farm to fork” strategy adopted by the European Commission assumes a significant limitation of the use of plant protection products and mineral fertilizers [2]. In order to prevent a reduction in sugar beet production, it is necessary to search for modern and environmentally safe production methods. An innovative method to limit their impact on sugar yields is the foliar application of products containing silicon (Si) [3]. This treatment primarily increases the tolerance of plant to water stress. It contributes to the growth of the root yield and usually does not significantly affect the technological quality of the roots, which in turn increases the biological yield of sugar and pure sugar yield.

It is commonly believed that the best effects are obtained with foliar silicon in the form of stabilized orthosilicic acid [4]. The research results show that good results in sugar beet production can be obtained by using marine calcite [5,6,7,8], a mixture of orthosilicic and polysilicic acids [4,8], potassium silicate [9] or silica nanoparticles [10,11].

The aim of the study is to assess the effectiveness of the foliar application of silicon-containing products in the form of orthosilicic acid, a mixture of orthosilicic and polysilicic acid and calcium silicate on the root yield, technological quality, biological sugar yield and pure sugar yield and indicate which effect is the strongest for the studied traits.

The following research hypothesis was assumed: the foliar application of various forms of silicon in sugar beet cultivation has a diversified impact on the root yield, technological quality as well as the biological and pure yield of sugar.

2. Materials and Methods

The experiment was conducted in 2017–2019 in the south-eastern region of Poland, in Sahryń (50°41′ N, 23°46′ E). The soil type was Calcic Chernozem (Aric, Siltic) (silty clay loam: clay—34%; sand—14%; silt—52%) [12].

The chemical analyses were conducted at District Chemical and Agricultural Stations in Warsaw, Wesoła. The soil’s physico-chemical properties were evaluated using standard methods, i.e., pH_KCl was measured potentiometrically in 1 M KCl [13]; oil organic carbon (SOC) was measured using Tiurin’s method [14]; nitrate nitrogen (N-NO₃) and ammonium nitrogen (N-NH₄) were extracted with 1% potassium sulphate and then measured with the use of the continuous flow analysis method [15]; phosphorus (P) was measured by the Egner–Riehm DL method [16]; potassium (K) was extracted with calcium lactate and measured by the flame photometry method [17]; magnesium (Mg) was extracted with calcium chloride and evaluated by flame atomic absorption spectrometry [18]; boron (B) was measured with the use of the spectrophotometric method [19]; copper (Cu) was extracted using hydrochloric acid and evaluated by flame atomic absorption spectrometry [20]; iron (Fe) was extracted with hydrochloric acid and evaluated by flame atomic absorption spectrometry [21]; manganese (Mn) was extracted with hydrochloric acid and evaluated by flame atomic absorption spectrometry [22]; and zinc (Zn) was extracted with hydrochloric acid and evaluated by flame atomic absorption spectrometry [23].

The soil properties are presented in

Table 1. Soil properties before sugar beet sowing in years 2016–2018.

Location	pHKCl	SOC, %	mg kg−1
			N-NO3	N-NH4	P	K	Mg	B	Cu	Fe	Mn	Zn
2016	7.3	2.11	81.5	5.02	46.2	104	71	2.8	6.4	540	164	6.3
2017	7.5	1.66	36.2	1.58	86.8	62	69	2.2	7.3	490	167	5.9
2018	7.3	2.76	18.4	3.11	90.7	133	99	5.6	8.8	630	157	8.0

.

The amount of precipitation in the period from April to October in 2018 alone was higher than the multi-year average (Figure 1). The highest rainfall deficit in June occurred in 2017, in July in 2019 and in August in 2017–2018. In 2018, there was a higher average temperature in each month compared to the multi-year period; in 2017 it was June, August–October; and in 2019, April, June–August and October.

After the rapeseed harvest, the straw was mixed with the soil with a stubble aggregate. This treatment was repeated twice more to destroy weeds and rape seed. Finally, a third tillage was also aimed for mixing phosphorus–potassium fertilizers with the soil. In autumn, Polifoska 6 fertilizer (6% N in ammonium form, 8.7% P as mono and diammonium phosphate, 24.9% K as potassium chloride and 2.8% S as sulphate) was applied at a dose of 400 kg ha⁻¹ and potassium chloride (49.8% of K) at a dose of 300 kg ha⁻¹. In spring, Saletrzak Standard 27 with boron (13.5% N in the ammonium form and 13.5% N in the nitrate form, 1.4% Ca, 2.4% Mg, 0.2% B) was spread at the dose of 500 kg ha⁻¹ and immediately mixed with the soil using a tilling set. Overall, the following were applied to the soil in the experiment (kg ha⁻¹): N—159; P—34.8; K—249; S—11.2; Ca—7; Mg—12 and B—1. Just before sowing, the soil was tilled shallowly with a cultivating unit.

Due to the varied course of spring weather, the sugar beet cultivar was sown at different times: in 2017, on 31 March; in 2018, on 11 April; and in 2019, on 30 March. The distances in the rows were 18 cm, the row spacing was 45 cm and the sowing depth was 2–2.5 cm. The Toleranza KWS variety was used in the experiment. Toleranza KWS (breeding company: KWS SAAT SE & Co. (Einbeck, Germany)) is a diploid cultivar, normal type (N), which was entered into the Polish National Register in 2015. The cultivar is characterized by high yields of roots and sugar. The technological quality of roots is high; sugar content is slightly above the standard; α-amino nitrogen content is quite low; potassium content is quite high; sodium content is quite low. Resistance to Cercospora beticola Sacc. is quite high. Resistance to beetroot nematode is declared by the breeder [24].

The sugar beet was harvested on 12 October 2017; 6 October 2018; and 26 September 2019.

At the six-leaf stage of sugar beet (Biologische Bundesanstalt, Bundessortenamt und Chemical Industry growth scale—BBCH 16) and 14 days later, foliar nutrition was applied with micronutrient fertilizers containing boron (2 × 300 g ha⁻¹ B). Standard protection against weeds, diseases, and pests was performed in accordance with the recommendations of the Institute of Plant Protection—National Research Institute in Poznań.

Three silicon-containing products were used in the experiment: ZumSil (88.1 g Si L⁻¹ in the form of orthosilicic acid, pH 9), Optysil (Si—94 g L⁻¹; Fe—24 g L⁻¹; a mixture of orthosilicic and polysilicic acid, neutral) and Barrier Si-Ca (calcium silicate; SiO₂—336 g L⁻¹; Ca—207 g L⁻¹; pH 9.5–11.0) (

Table 2. Treatments used in the experiment (2017–2019).

Treatment	Description
0—control	Without foliar application
1	ZumSil 3 × 0.5 L ha−1: at 6 leaves stage (BBCH 16) + 7 days later + 14 days later
2	Optysil 3 × 0.5 L ha−1: at 6 leaves stage (BBCH 16) + 7 days later + 14 days later
3	Barrier Si-Ca 3 × 1 L ha−1: at 6 leaves stage (BBCH 16) + 7 days later + 14 days later

). In each treatment, 250 L of water ha⁻¹ was used. In variant no. 1 and 2, the concentration of the working liquid was 0.2%, and in variant no. 3, it was 0.4%.

The foliar application was performed in 2017 on 27 May, 3 June and 10 June; in 2018 on 22 May, 29 May and 5 June; and in 2019 on 25 May, 31 May and 8 June. Spraying was performed with an Apollo trailed sprayer (Krukowiak).

Four replications for each treatment were conducted, i.e., 16 plots in each season (4 plots × 4 treatments). Each individual plot had area of 43.2 m² (16 m of length and 2.7 m of width) consisting of six rows of plants. Three middle rows were used for the harvesting and evaluation of yield. During harvest, the plants were topped by hand on the three middle rows, and the leaves were weighed. The roots were then counted, dug up and weighed. At harvest, each plot was treated in accordance with the Polish Standard [25]. The technological quality of the roots was evaluated on the automatic Venema technological line in the Kutno Sugar Beet Breeding Company in Straszków Poland [26]: the sugar content polarimetrically [27], the K and Na by photoelectric flame photometry [27] and the α-amino nitrogen by fluorometric methods [28].

The measurements performed in the experiments were as follows: plant density at harvest (thousand plants ha^–1); root yield (t ha^–1); yield of leaves (t ha^–1); yield of fresh biomass (t ha^–1) as a sum of the root yield (t ha^–1) and yield of leaves (t ha^–1); harvest index (HI) as a ratio of root yield to fresh biomass; foliage coefficient as a ratio of yield of leaves to root yield; fresh biomass of root (kg) as a ratio of root yield (kg) and number of plants per plot at harvest; fresh biomass of leaves per plant (kg) as a ratio of (kg) and number of plants per plot at harvest; plant fresh weight (kg) as the sum of fresh root mass (kg) and leaves of a single plant (kg); content of sucrose in roots (%); content of α-amino nitrogen in the roots (mmol kg^–1); content of potassium (K) in the roots (mmol kg^–1); content of sodium (Na) in the roots (mmol kg^–1); biological yield of sugar (t ha^–1) = product of root yield (t ha^–1) and content of sugar in roots (%); pure sugar yield (t ha^–1) = root yield (t ha^–1) × [content of sugar (%)—sugar yield losses (%)] [29]; sugar yield losses (%) = standard molasses losses (%) + 0.6 (%) [29]; standard molasses losses (%) = 0.012 × (K + Na) + 0.024 (α-amino nitrogen) + 0.48, where the content of K, Na and α-amino nitrogen are given in mmol kg^–1 of pulp [29]; refined sugar content (%) = sucrose content (%)—sugar yield losses (%); sugar productivity (%) = refined sugar content (%)/sugar content (%) × 100; and alkalinity coefficient W_A = (content of K (mmol kg^–1) + content of Na (mmol kg^–1))/content of α-amino nitrogen (mmol kg^–1) [30].

Analysis of variance was applied for the evaluation of the effects of the studied factors and their interaction. Multiple comparisons of means using Tukey’s HSD procedure were performed for separation of the homogenous groups of the means. The analyses were conducted using Statistica 13 program (TIBCO Software Inc.) at significance level 0.05. Basic statistical parameters, i.e., range, standard deviation (SD) and coefficient of variation (CV), were calculated.

3. Results

Results of the analysis of variance proved a significant effect of the year for almost all studied traits (

Table 3. Results based on analysis of variance (ANOVA) presenting effects (p-values) of the factors and their interaction on the yield, the technological quality of the roots and traits of sugar beet plants (2017–2019).

Trait	Effect (p-Value)
	Year	Treatmant	Year × Treatment
Plant density at harvest, thousand plants ha−1	0.001	0.639	<0.001
Yield of roots, t ha−1	<0.001	0.036	0.697
Yield of leaves, t ha−1	<0.001	0.050	0.023
Yield of roots and leaves, t ha−1	0.084	0.029	0.439
Biological yield of sugar, t ha−1	<0.001	0.031	0.447
Pure sugar yield, t ha−1	<0.001	0.076	0.429
Harvest Index	<0.001	0.799	0.024
Foliage coefficient	<0.001	0.727	0.028
Content of sucrose in roots, %	<0.001	<0.001	0.001
The content of α-amino nitrogen in the roots, mmol kg−1	<0.001	0.015	0.023
Potassium content in the roots, mmol kg−1	0.026	0.011	0.006
Sodium content in the roots, mmol kg−1	0.015	0.045	0.014
Standard molasses losses, %	<0.001	0.015	0.017
Sugar yield losses, %	<0.001	0.015	0.017
Refined sugar content, %	<0.001	<0.001	0.001
Sugar productivity, %	<0.001	0.001	0.002
Alkalinity coefficient	<0.001	<0.001	0.003
Fresh root mass, kg	<0.001	0.045	0.001
Fresh mass of the leaves of the plant, kg	<0.001	0.292	0.935
Fresh plant biomass, kg	0.683	0.038	0.084

). The only exception was fresh plant biomass. A significant effect of the treatment was observed for most of the studied traits (exceptions: harvest index, foliage coefficient and fresh mass of the leaves of the plant). A significant interaction (year × treatment) was observed for most of the traits, which means that the effect of the treatment was dependent on the year. The exceptions were the yield of roots, yield of roots and leaves, biological yield of sugar, pure sugar yield, fresh mass of the leaves of the plant and fresh plant biomass. Plant density during harvest was not significantly different between particular treatments and ranged from 88.8 to 92.0 thousand plants ha⁻¹ (

Table 4. The influence of various forms of silicon on the yield, the technological quality of the roots and the traits of sugar beet plants (2017–2019).

Trait	Treatment
	0	1	2	3
Plant density at harvest, thousand plants ha−1	89.12 a 1	92.01 a	90.39 a	88.77 a
Yield of roots, t ha−1	73.09 a	81.14 b	80.94 b	81.64 b
Yield of leaves, t ha−1	45.05 a	49.66 ab	52.43 b	49.38 ab
Yield of roots and leaves, t ha−1	118.14 a	130.80 b	133.37 b	131.02 b
Biological yield of sugar, t ha−1	13.06 a	14.16 ab	14.65 b	14.58 b
Pure sugar yield, t ha−1	11.57 a	12.40 ab	12.94 b	12.88 b
Harvest Index	0.616 a	0.621 a	0.607 a	0.621 a
Foliage coefficient	0.656 a	0.653 a	0.684 a	0.628 a
Content of sucrose in roots, %	17.65 b	17.28 a	17.96 c	17.58 b
The content of α-amino nitrogen in the roots, mmol kg−1	24.65 a	27.90 b	25.68 ab	23.92 a
Potassium content in the roots, mmol kg−1	29.45 a	32.10 b	30.07 a	32.08 b
Sodium content in the roots, mmol kg−1	2.72 a	3.48 b	2.63 a	2.83 ab
Standard molasses losses, %	1.46 a	1.58 b	1.49 a	1.47 a
Sugar yield losses, %	2.06 a	2.18 b	2.09 a	2.07 a
Refined sugar content, %	15.59 bc	15.10 a	15.87 c	15.51 b
Sugar productivity, %	88.23 b	87.35 a	88.33 b	88.16 b
Alkalinity coefficient	1.32 a	1.30 a	1.33 a	1.50 b
Fresh root mass, kg	0.820 a	0.895 ab	0.897 ab	0.923 b
Fresh mass of the leaves of the plant, kg	0.512 a	0.539 a	0.580 a	0.571 a
Fresh plant biomass, kg	1.332 a	1.434 b	1.477 b	1.494 b

¹ The same letters within rows indicate a lack of significant differences between means at α = 0.05.

). All treatments with silicon foliar application contributed to a significant increase in the root yield (10.7–11.7%) compared to the control object, but their effect was not significantly different. A significant increase in the yield of leaves was observed only in treatment no. 2. A significant increase in the biological yield of sugar was found in treatments no. 2 (by 12.2%) and 3 (by 11.6%), and a slightly smaller, but statistically insignificant increase, in treatment no. 1 (by 8.4%) was observed. It was not significantly different in the case of pure sugar yield, where the gains amounted to 11.8%, 11.3% and 7.2%, respectively.

There were no significant differences in the values of the Harvest Index and foliage coefficient.

The impact of the assessed foliar applications on the sucrose content in sugar beet roots was varied. Treatment no. 1 significantly increased it, and treatment no. 2 significantly decreased it compared to the control object. On the other hand, treatment no. 3 had no significant effect.

The effect of the tested treatments on the content of molasses-forming components in the roots was also diversified.

The content of α-amino nitrogen in relation to treatment no. 0 was significantly increased only by treatment no. 1, potassium content for treatment no. 1 and 3 and sodium for treatment no. 1.

Treatment no. 1 significantly increased the standard of molasses losses and sugar yield losses and decreased refined sugar content and sugar productivity. On the other hand, treatment no. 2 significantly increased refined sugar content, and treatment no. 3 increased alkalinity coefficient.

The differences in the root yield, biological sugar yield and pure sugar yield between the treatments with foliar application and the control treatment in individual years were insignificant (Figure 2). The sucrose content in the roots in 2017 for treatment no. 3 was lower than for treatment no. 0 (similarly to treatment no. 1 in 2019). In 2018, treatment no. 2 and 3 showed a significantly higher sucrose content compared to the control object.

Among the examined traits, the highest variability was found in the foliage coefficient (CV = 37.4%), and the lowest variability was found in the sugar productivity (CV = 1.38%) (

Table 5. Descriptive statistics for all experiments with sugar beet (2017–2019).

Trait	Mean	Minimum	Maximum	Standard Deviation (SD)	Coefficient of Variation (CV), %
Plant density at harvest, thousand plants ha−1	90.08	69.44	105.56	9.64	10.70
Yield of roots, t ha−1	79.21	52.22	103.47	14.45	18.25
Yield of leaves, t ha−1	49.13	29.65	79.86	11.77	23.95
Yield of roots and leaves, t ha−1	128.34	98,92	157,78	14,37	11,20
Biological yield of sugar, t ha−1	14.11	8.14	18.85	3.30	23.40
Pure sugar yield, t ha−1	12.45	7.00	16.76	2.99	24.03
Harvest Index	0.616	0.471	0.742	0.084	13.71
Foliage coefficient	0.655	0.348	1.123	0.245	37.42
Content of sucrose in roots, %	17.62	15.42	19.23	1.27	7.21
The content of α-amino nitrogen in the roots, mmol kg−1	25.54	17.70	39.00	4.91	19.23
Potassium content in the roots, mmol kg−1	30.93	24.40	36.20	2.97	9.62
Sodium content in the roots, mmol kg−1	2.92	0.40	4.80	0.98	33.69
Standard molasses losses, %	1.50	1.30	1.84	0.14	9.07
Sugar yield losses, %	2.10	1.90	2.44	0.14	6.48
Refined sugar content, %	15.52	13.27	17.31	1.29	8.32
Sugar productivity, %	88.02	86.03	89.99	1.21	1.38
Alkalinity coefficient	1.36	0.91	2.01	0.24	17.46
Fresh root mass, kg	0.884	0.559	1.227	0.159	17.96
The fresh mass of the leaves of the plant, kg	0.551	0.314	0.892	0.142	25.83
Fresh plant biomass, kg	1.435	1.074	1.803	0.173	12.04

).

4. Discussion

The weather conditions have a strong influence on the sugar beet yield [31,32,33,34,35]. In our research, the highest yields of roots and sugar were obtained in 2017 and 2019. This was definitely lower in 2018, which was characterized by a higher temperature each month during the vegetation period in comparison to the multi-year average.

The length of the growing season is determined by the sowing and harvesting dates. The sowing date depends on the course of weather conditions in early spring. In our research, the length of the growing season was 195 days in 2017, 178 days in 2018 and 180 days in 2019. The variation in the harvest date was caused by the weather conditions in this period.

The final trait in sugar beet production is pure sugar yield. It depends primarily on the root yield, and the effect of the sucrose content in the roots (positive effect) and molasses-forming components (negative effect) is much smaller. With a similar plant density during harvesting, the root yield and, consequently, the pure sugar yield are determined by the fresh weight of the root [35,36]. Our research confirmed the beneficial effect of foliar application of the tested silicon forms on fresh root mass and root yield.

In the available literature, there are only a few publications on the effects of the foliar application of silicon in the cultivation of sugar beet. The increases in the yield of sugar beet roots obtained in our research as a result of the foliar application of silicon-containing products were lower than those obtained in previous studies (

Table 6. Results of studies on foliar application of various forms of silicon in the cultivation of sugar beet.

Form of Silicon Applied as a Foliar Application	Maximum Difference in Control Ratio (No Foliar Application)				References
	%			p.p.
	Yield of Roots	Biological Yield of Sugar	Pure Sugar Yield	Content of Sucrose
Marine calcite	0.0	+2.7	+4.9	+0.5	[7]
	+12.0	+9.7	+8.6	−0.6	[4]
	+14.6	+21.8	+17.8	+0.6	[5]
	+16.2	+18.1	+17.7	−0.6	[8]
	+21.8	+24.8	+25.2	+0.2	[6]
Orthosilicic acid stabilized with choline with the addition of calcium	+25.1	+23.2	+22.2	−0.7	[8]
Mixture of orthosilicic and polysilicic acids with the addition of iron	+15.9	+15.7	+15.6	+0.2	[8]
	+18.9	+16.4	+14.1	−0.4	[4]
Potassium silicate	+20.4	+21.9	+23.3	+0.5	[9]
Silica nanoparticles	+6.5	+6.2	+6.8	−0.1	[11]
	+12.8	+19.6	+19.6	+1.1	[10]

). Only some studies on production fields did not increase the root yield after the foliar application of marine calcite [7]. The increase in the yield after the application of calcium silicate, obtained in the authors’ research, contradicts some opinions that silicates applied on leaves only reduce infections and have no influence on the yield [37]. In the case of the biological yield of sugar and pure sugar yield, the observed gains were lower than in previous studies with some exceptions. The beneficial effect of nano-Si on the increase drought tolerance of sugar beet depends on the selection of an appropriate concentration, as too high a concentration may be toxic for plants subjected to severe drought stress [38]. Previous studies have shown that the foliar application of various forms of silicon was profitable for sugar beet producers [39,40].

A crop with a production technology similar to that of sugar beet is potato. In the case of potato, most studies proved a positive effect of the foliar application of various forms of silicon on the tuber yield, and the dry matter content in tubers was observed (

Table 7. Results of studies on foliar application of various forms of silicon in the cultivation of potato.

Form of Silicon Applied as a Foliar Application	Maximum Difference in Control Ratio (No Foliar Application)			References
	%		p.p.
	Yield of Tubers	Commercial Yield of Tubers	Content of Dry Matter
Marine calcite	+9.0	+11.0	No data	[41]
Orthosilicic acid stabilized with choline with the addition of calcium	−13.6	No data	−0.7	[42]
Orthosilicic acid + microelements (Cu, Zn, B, Mo)	+10.8	No data	No data	[43]
Orthosilicic acid + microelements (Cu, Zn, B, Mo)	+12.9	No data	+0.5	[44]
Orthosilicic acid	+21.0	+18.3	+0.1	[45]
Oligomeric silicic acid and boric acid	+6.5	No data	No data	[46]
Silicic acid (type of SA is not mentioned)	+12.8	No data	No data	[47]
Orthosilicic and disilicic acid	similar to well-watered potato plants (water-stress)	No data	No data	[48]

).

The main feature of the technological quality of sugar beet roots is the sucrose content. In our research, this increased after the foliar application of orthosilicic acid, decreased after using a mixture of orthosilicic and polysilicic acids and did not change significantly as a result of the use of calcium silicate. This is confirmed by previous research results, in which the effect of the foliar application of silicon-containing products was also varied (Table 6).

Among the molasses-forming components, the most harmful is α-amino nitrogen, which limits the extraction of sugar from the roots the most and increases its losses. In our research, only the foliar application of orthosilicic acid resulted in a significant increase in the content of this component. In previous studies, the effect of the foliar application of silicon, regardless of its form and the content of other components in foliar fertilizers and stimulators, caused slight changes in the content of α-amino nitrogen in sugar beet roots.

The varied influence of the foliar application of silicon could result from the different course of weather conditions during the growing season in individual years of the study. In general, the less favourable they were to plant growth and development, the more pronounced the effects were.

The results of this study allowed us to reject the research hypothesis that various forms of silicon applied in foliar application have a different effect on the yield of roots, their technological quality and biological sugar yield and pure sugar yield.

5. Conclusions

Practical Implications of this Study

The conclusion is that for agricultural practice, the use of all three forms of silicon tested in the experiments can be recommended. It is impossible to indicate which of the tested forms of silicon has the most beneficial effect. This is useful for farmers as it guarantees a wide range of products that can be used for application.

The presented research results prove that the foliar application of silicon, regardless of its form, has a beneficial effect on the root yield, biological sugar yield and pure sugar yield. The effect of the application of silicon depends on weather conditions in years of the study as well on the interaction between weather conditions and the studied treatments.

Further research should focus on determining the optimal date of foliar application of the products with silicon depending on the weather conditions. Unfortunately, such research is difficult to perform in field conditions; it can be performed under controlled laboratory conditions, but the results thus obtained are often not confirmed in field conditions.