First Report: Senna multijuga Subsp. multijuga (Fabales: Fabaceae) as an Attractant and Bioinsecticide for Oryctes rhinoceros (Coleoptera: Scarabaeidae)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of “Hujan Emas” (Golden Rain) Plants
2.1.1. Morphological Characteristics
2.1.2. Molecular Identification
- DNA Extraction
- DNA Amplification
- Sequencing and Analysis of Results
2.2. The Potential of the “Hujan Emas” (Golden Rain) Plant as an Attractant for O. rhinoceros Beetles
2.3. Laboratory Testing of the Plant Extract’s Ability as a Bioinsecticide against O. rhinoceros
2.3.1. Preparation of the Golden Rain Plant Extract
2.3.2. Experimental Design
2.3.3. Preparation of Test Insects
2.3.4. Application of Plant Extracts
2.3.5. Observations and Data Collection
2.3.6. Data Analysis
3. Results
3.1. Senna multijuga Has the Identity of the “Hujan Emas” (Golden Rain) Plant
3.1.1. Morphological Observations Indicate That the Golden Rain Plant Is Senna multijuga
3.1.2. Molecular Identification Confirmed That the Golden Rain Plant Is Senna multijuga
3.2. Senna multijuga Has the Ability to Attract Adult O. rhinoceros
3.3. Senna multijuga Has Potential as a Bioinsecticide for O. rhinoceros
- Mortality of Oryctes rhinoceros larvae
- Mortality of Oryctes rhinoceros beetle
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence (5′–3′) | Length of the PCR Product (bp) |
---|---|---|
matKsenF | TAAGGYTCTTTCTTTATGAG | 749 |
matKsenR | ATACAAACTCTTTTTTGTTG | |
rps16senF | GCCGTGGATTCTTACATCCA | 825 |
rps16senR | ACGTATAAGAAGTTTTCTCC | |
rpl16senF | CTCCTCGCGAATGAAASGAT | 846 |
rpl16senR | GATATATTGGCGATATTATT |
Gene | Identity | Total Score | Query Coverage | Percentage Identity | Acc. No |
---|---|---|---|---|---|
MaturaseK (MatK) | Senna multijuga | 1341 | 100% | 99.86% | KX538469 |
chloroplast rps16 (rps16) | Senna multijuga var. multijuga | 1517 | 100% | 99.76% | AM086962 |
chloroplast rpl16 (rpl16) | Senna multijuga var. multijuga | 1568 | 100% | 99.77% | AM08676 |
Source | DF | Anova SS | Mean Square | F Value | Pr > F |
---|---|---|---|---|---|
Block | 3 | 468.750000 | 156.250000 | 0.84 | 0.4872 |
Plant part | 3 | 1718.750000 | 572.916667 | 3.08 | 0.0496 |
Solvent | 1 | 78.125000 | 78.125000 | 0.42 | 0.5240 |
Plant part × Solvent | 3 | 78.125000 | 26.041667 | 0.14 | 0.9349 |
Source | DF | Anova SS | Mean Square | F Value | Pr > F |
---|---|---|---|---|---|
Block | 3 | 58.593750 | 19.531250 | 2.33 | 0.1037 |
Plant part | 3 | 1638.843750 | 546.281250 | 66.12 | <0001 |
Solvent | 1 | 175.781250 | 175.781250 | 20.96 | 0.0002 |
Plant part × Solvent | 3 | 176.343750 | 58.781250 | 7.01 | 0.0019 |
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Wandri, R.; Alam, S.; Ayundra, S.D.; Apriansa, A.; Asmono, D.; Subeki, S.; Fitriana, Y.; Hasibuan, R.; Suharjo, R. First Report: Senna multijuga Subsp. multijuga (Fabales: Fabaceae) as an Attractant and Bioinsecticide for Oryctes rhinoceros (Coleoptera: Scarabaeidae). Agriculture 2024, 14, 1477. https://doi.org/10.3390/agriculture14091477
Wandri R, Alam S, Ayundra SD, Apriansa A, Asmono D, Subeki S, Fitriana Y, Hasibuan R, Suharjo R. First Report: Senna multijuga Subsp. multijuga (Fabales: Fabaceae) as an Attractant and Bioinsecticide for Oryctes rhinoceros (Coleoptera: Scarabaeidae). Agriculture. 2024; 14(9):1477. https://doi.org/10.3390/agriculture14091477
Chicago/Turabian StyleWandri, Ruli, Samsu Alam, Shervinia Dwi Ayundra, Azharudin Apriansa, Dwi Asmono, Subeki Subeki, Yuyun Fitriana, Rosma Hasibuan, and Radix Suharjo. 2024. "First Report: Senna multijuga Subsp. multijuga (Fabales: Fabaceae) as an Attractant and Bioinsecticide for Oryctes rhinoceros (Coleoptera: Scarabaeidae)" Agriculture 14, no. 9: 1477. https://doi.org/10.3390/agriculture14091477